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Karakteristik, Warna, Dan Ukuran Mikroplastik Yang Ditemukan Pada Air Dan Kerang Hijau Di Pulau Pasaran, Lampung Romaskila, Ulfanida; Widiastuti, Endang Linirin; Susanto, Gregorius Nugroho; Damai, Abdullah Aman; Juliasih, Ni Luh Gede Ratna
Journal of Tropical Marine Science Vol 6 No 2 (2023): Journal of Tropical Marine Science
Publisher : Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/jour.trop.mar.sci.v6i2.4236

Plastic waste will be degraded into microplastic which has a size of ≤ 5 mm. These microplastic particles change in size and color due to various natural processes that occur in the environment. The purpose of this study was to determine the shape, color and size characteristics of microplastics found around Pasaran Island. Sampling was carried out by taking microplastic samples in water and green mussels around Pasaran Island. Based on the research results, microplastic fragments, films, fibers, granules and foam were found. Microplastic colors consist of black, blue, green, yellow, red, clear, and white. Microplastic fragments, films and fibers were found in the <50µm to 1000 - 5000 µm size group. Granular microplastic types were found in the <50 µm to 100 - 500 µm size group and foam types were found in the <50 µm to 500 - 1000 µm size group.

Activity of Mangrove-Derived Fusarium equiseti 20CB07RF Extract Against Clinical, Antibacterial-Resistant Pseudomonas aeruginosa Bahri, Syaiful; Setiawan, Wawan Abdullah; Setiawan, Fendi; Lutfiah, Rosyidatul; Juliasih, Ni Luh Gede Ratna; Ambarwati, Yuli; Ahmadi, Peni; Arai, Masayoshi; Hendri, John; Hadi, Sutopo; Setiawan, Andi
Science and Technology Indonesia Vol. 9 No. 3 (2024): July
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2024.9.3.594-604

Endophytic fungi originating from mangroves are potential sources of secondary metabolites with varying bioactivities. This research explores the bioactive metabolites produced by endophytes derived from mangrove plants. Endophytic fungi were collected from various parts of several mangrove plants (roots, stems, and leaves, as well as the surrounding mud). A total of 17 endophytics fungi were obtained. The isolates were derived from the leaves (1 isolate), stems (8 isolates), roots (5 isolates), and surrounding mud (3 isolates). A single fungal colony was cultured using solid-state fermentation for 14 days. The fermented fungal biomass was extracted using ethyl acetate (EtOAc) and evaluated for its antibacterial activity against clinical pathogenic bacteria. In the preliminary screening, the EtOAc extract of the CB07RF1 isolate exhibited notable growth-inhibitory effects against Pseudomonas aeruginosa. The isolate was verified by molecular identification using a study of the rDNA internal transcribed spacer (ITS) sequence, revealed that isolate CB07RF1 was very similar to Fusarium equiseti (99% similarity). Isolate 20CB07RF1, obtained by solid-state fermentation using a rice medium indicated as peptide compound group, and featured active components that exhibited potent growth inhibitory activity against Pseudomonas aeruginosa at a concentration of 12.5 mg/mL. This study demonstrates, for the first time, that Fusarium equiseti extracts grown in a rice medium contain antimicrobial compounds that can inhibit the growth of P. aeruginosa, an important clinical pathogen known for its antibacterial resistance. These findings accent mangrove endophytic fungi as important sources of bioactive compounds and will advance related research in the fields of biotechnology, pharmacology, and life sciences.

New Encapsulation of Fucoxanthin Isolated from Cyclotella striata by Nano Chitosan–Pectin using Ionic Gelation Method Nahrowi, Ridho; Solehati, Siti; Widyastuti, Widyastuti; Juliasih, Ni Luh Gede Ratna; Pandiangan, Kamisah Delilawati; Setiawan, Andi; Hendri, John
Science and Technology Indonesia Vol. 9 No. 3 (2024): July
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2024.9.3.517-528

Fucoxanthin is an anticancer, antioxidant, antimicrobial, and anti-inflammatory bioactive compound. Unfortunately, the conjugated double bonds of the fucoxanthin structure make it unstable, posing issues for product development, particularly with regard to shelf life. This research study aims to synthesize nano chitosan–pectin and encapsulate isolated fucoxanthin by nano chitosan–pectin using an ionic gelation method. Fucoxanthin was obtained through isolation of microalgae species Cyclotella striata. The best result of nanoparticle size using a particle size analyzer was chitosan:pectin 1 : 2 of 172 nm. Fourier transform infrared analysis showed that there was an interaction between chitosan–pectin and fucoxanthin, which was characterized by a shift in the C O absorption fucoxanthin from 1736 to 1632 cm-1. The result of morphological analysis of nano chitosan–pectin–fucoxanthin using a scanning electron microscopeshows a spherical morphology with a size between 140 and 265 nm. The result of encapsulation efficiency was 75.18%, whereas encapsulation stability increased fucoxanthin oxidation half-life 4.7 times longer than that of unencapsulated fucoxanthin. The nano chitosan pectin could be utilized as a matrix conjugate to increase the stability of fucoxanthin significantly by encapsulation. This information is expected to be useful in developing encapsulation applications for unstable compounds.

Novel Micrococcus unila to Produce Glucosamine by Solid-state Fermentation of Shrimp Shell Waste Setiawan, Wawan Abdullah; Setiawan, Andi; Salsabila, Nafila Khansa; Widyastuti, Widyastuti; Laila, Aspita; Juliasih, Ni Luh Gede Ratna; Irawan, Bambang; Ahmadi, Peni; Apriliana, Ety; Arai, Masayoshi; Hendri, John
Science and Technology Indonesia Vol. 9 No. 4 (2024): October
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2024.9.4.779-789

This study aimed to assess glucosamine production through enzymatic activity, utilizing actinomycetes sourced from shrimp shell waste (SSW) in a solid-state fermentation (SSF) process. A total of 16 actinomycetes underwent chitinase activity screening, and the strain exhibiting the highest chitinolytic index was chosen for subsequent morphological and phylogenetic analyses. High Performance Liquid Chromatography (HPLC) was employed to analyze glucosamine produced from the bioconversion of SSW via SSF. Optimal conditions for glucosamine production were determined by varying time, pH, and temperature. Isolate 18D36-A2 showed the highest chitinolytic index of 1.02 in the 32-mm clean zone. Phylogenetic analysis revealed 97% similarity to the genus Micrococcus, identifying it as a novel Micrococcus unila strain 18D36-A2 and deposited in GenBank. This isolate effectively converted shrimp shells. The findings showcase the bioconversion of SSW to glucosamine through SSF using the Micrococcus unila 18D36-A2. Furthermore, this study establishes a foundation for future research on environmentally friendly and sustainable designs for glucosamine production.

Pendampingan Pembuatan Produk SANTRI (Sabun Cair Antibakteri) Berbasis Ekstrak Daun Sirih pada Kelompok PKK Rinawati, Rinawati; Akmal, Jamiatul; Juliasih, Ni Luh Gede Ratna; Ning Tyas, Marsanda Nur Wahyu; Kiswandono, Agung Abadi
Jurnal Pengabdian kepada Masyarakat (ABDIKEMAS) Vol 6 No 2 (2024): Jurnal Pengabdian kepada Masyarakat (ABDIKEMAS)
Publisher : Pusat Penelitian dan Pengabdian kepada Masyarakat, Politeknik Kesehatatan Kemenkes Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36086/j.abdikemas.v6i2.2512

The mentoring activity for making dishwashing soap with betel leaf extract in Gebang Village aims to increase the independence and welfare of the local community, especially for Micro, Small, and Medium Enterprises (MSMEs). This program is implemented as an effort to optimize local natural potential, especially betel leaf plants which are known to have antibacterial and natural cleaning properties. This activity involves several stages, starting from education about the benefits of betel leaves, technical training in making soap, to mentoring in aspects of product packaging and marketing.The method used includes a participatory approach, where the community is invited to be actively involved in every process, so that a sustainable transfer of knowledge and skills is created. The results of this activity show that the community is able to produce effective, environmentally friendly dishwashing soap that is competitive in the local market. In addition, participants are also given training on simple business management, including bookkeeping, determining selling prices, and marketing strategies.This mentoring has a positive impact on increasing the capacity of individuals and the village economy, especially in creating local superior products with high selling value. It is hoped that with these new skills, the community can improve their standard of living and make making dishwashing soap one of the sustainable businesses in the village.

Pelatihan Pembuatan Detergen Cair Ramah Lingkungan Berbasis Eco Enzyme Pada Kelompok PKK Desa Rejomulyo Husin, Nurhasanah; Ginting, Simparmin; Juliasih, Ni Luh Gede Ratna; Kiswandono, Agung Abadi; Bella, Adelia Citra; Rezamita Hapsari, Roro; Adelia, Riska; Izzati, Alya Nurul; Sari, Dian Nopita; Al Madya, Vitha
LOSARI: Jurnal Pengabdian Kepada Masyarakat Vol. 6 No. 2 (2024): Vol. 6 No. 2 (2024) : Desember 2024
Publisher : LOSARI DIGITAL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53860/losari.v6i2.406

Eco enzyme merupakan produk pengolahan limbah rumah tangga yang berasal dari sisa sayuran atau buah-buahan. Eco enzyme dapat dijadikan sebagai bahan aktif untuk membuat produk kebersihan rumah tangga diantaranya detergen cair ramah lingkungan. Pembuatan detergen cair berbasis eco enzym memiliki peluang untuk dijadikan sebagai kegiatan home industry. Tujuan kegiatan ini adalah memberikan pelatihan kepada kelompok PKK Desa Rejomulyo dalam pembuatan detergen cair ramah lingkungan dengan bahan aktif eco enzyme. Tahapan pelaksanaan kegiatan terdiri dari ceramah, tanya jawab serta pelatihan pembuatan detergen cair. Hasil kegiatan diperoleh produk detergen cair ramah lingkungan yang dapat dikonsumsi oleh masyarakat di Desa Rejomulyo. Detergen cair ramah lungkungan yang dihasilkan dari kegiatan ini diharapkan menjadi nilai tambah bagi anggota PKK untuk mengembangkan produk usaha ekonomi kreatif berbasis home industry. Hasil akhir dari pengabdian ini adalah terwujudnya usaha industri rumah tangga berbasis eco enzyme dan diperolehnya variasi produk kebersihan rumah tangga dari PKK Desa Rejomulyo.

Potensi Antikanker Pada Ekstrak Etanol Makroalga Caulerpa racemosa Menggunakan Metode Brine Shrimp Lethality Test (BSLT) Khasana, Devi Ardiyati; Widiastuti, Endang Linirin; Nurcahyani, Nuning; Setyawan, Agus; Juliasih, Ni Luh Gede Ratna
Journal of Tropical Marine Science Vol 8 No 1 (2025): Journal of Tropical Marine Science (on going)
Publisher : Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/jour.trop.mar.sci.v8i1.5759

Caulerpa racemosa is one of the green macroalgae that is widely distributed in the coastal areas of Lampung Province and needs to be explored as a phytopharmaceutical ingredient because it is thought to have bioactive compounds. In this study, we also used taurine, which is known to prevent cell and tissue damage due to oxidative processes, to explore its potential as an anticancer. The initial stage of this research is phytochemical screening of 96% ethanol extract of Caulerpa racemosa and contains phenolic compounds . alkaloids, saponins, flavonoids, tannins, steroids and terpenoids. The toxicity test of Caulerpa racemosa ethanol extract and taurine used the BSLT method (Brine Shrimp Lethality Test). In this study, 7 concentrations were used, namely 0 ppm (control); 62.5; 125; 250; 500; 1000; and 2000 for both Caulerpa racemosa and taurine ethanol extracts with 5 repetitions. Meanwhile, the toxicity test results showed that the LC50 value of Caulerpa racemosa ethanol extract was 194.14 ppm and 170.69 ppm for taurine, this values are between 100 ppm - 1000 ppm of Caulerpa racemosa ethanol and taurine indicate that both material have potential anticancer activity with low toxicity.

Potensi Antikanker Pada Ekstrak Etanol Makroalga Caulerpa racemosa Menggunakan Metode Brine Shrimp Lethality Test (BSLT) Khasana, Devi Ardiyati; Widiastuti, Endang Linirin; Nurcahyani, Nuning; Setyawan, Agus; Juliasih, Ni Luh Gede Ratna
Journal of Tropical Marine Science Vol 8 No 1 (2025): Journal of Tropical Marine Science (on going)
Publisher : Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/jour.trop.mar.sci.v8i1.5759

Caulerpa racemosa is one of the green macroalgae that is widely distributed in the coastal areas of Lampung Province and needs to be explored as a phytopharmaceutical ingredient because it is thought to have bioactive compounds. In this study, we also used taurine, which is known to prevent cell and tissue damage due to oxidative processes, to explore its potential as an anticancer. The initial stage of this research is phytochemical screening of 96% ethanol extract of Caulerpa racemosa and contains phenolic compounds . alkaloids, saponins, flavonoids, tannins, steroids and terpenoids. The toxicity test of Caulerpa racemosa ethanol extract and taurine used the BSLT method (Brine Shrimp Lethality Test). In this study, 7 concentrations were used, namely 0 ppm (control); 62.5; 125; 250; 500; 1000; and 2000 for both Caulerpa racemosa and taurine ethanol extracts with 5 repetitions. Meanwhile, the toxicity test results showed that the LC50 value of Caulerpa racemosa ethanol extract was 194.14 ppm and 170.69 ppm for taurine, this values are between 100 ppm - 1000 ppm of Caulerpa racemosa ethanol and taurine indicate that both material have potential anticancer activity with low toxicity.

Karakterisasi Bakteri Patogen Vibrio Parahaemolyticus Pada Bawal Bintang (Trachinotus blochii) Dari Perairan Teluk Hurun, Lampung Saputra, Rakhmat Hadi; Setiawan, Agus; Sumardi, Sumardi; Adiputra, Yudha Trinoegraha; Juliasih, Ni Luh Gede Ratna; setiawan, agus setiawan
Journal of Tropical Marine Science Vol 8 No 2 (2025): Journal of Tropical Marine Science
Publisher : Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/jour.trop.mar.sci.v18i2.6949

Industri perikanan budidaya laut menghadapi tantangan besar dalam pengendalian penyakit yang disebabkan oleh bakteri patogen. Salah satu patogen yang sering menyerang ikan budidaya adalah Vibrio parahaemolyticus, yang dapat menyebabkan vibriosis pada ikan laut. Penelitian ini bertujuan untuk mengetahui karakter bakteri patogen Vibrio parahaemolyticus yang ditemukan pada ikan Bawal Bintang (Trachinotus blochii) di perairan Teluk Hurun, Lampung, serta menguji potensi antibakterinya. Karakterisasi bakteri V. parahaemolyticus meliputi uji media selektif CHROMagar Vibrio (CV), konfirmasi gen toxR dengan metode PCR, aktivitas hemolitik pada medium blood agar plate (BAP) uji biokimia dengan metode API 20E, uji patogenisitas secara in vio pada bawal bintang, dan uji sensitivitas terhadap antibiotik dan bahan antimikrobia. Hasil penelitian menunjukkan bakteri V. parahaemolyticus tumbuh pada media CAV dengan warna biru kemerahan, teramplifikasi gen toxR, hemolitik positif, morfologi koloni berwarna krem dengan tepi bergelombang, mampu memfermentasi beberapa karbohidrat, termasuk glukosa, mannitol, dan sukrosa. Uji patogenisitas in vivo pada ikan bawal bintang menunjukkan gejala klinis berupa lesu, peradangan pada sirip, dan perdarahan, yang menandakan bahwa Vibrio parahaemolyticus merupakan patogen penyebab vibriosis pada ikan. Uji antibakteri menunjukkan bahwa enrofloxacin adalah antibiotik yang paling efektif, diikuti oleh tetrasiklin dan oksitetrasiklin. Ekstrak jintan hitam menunjukkan hasil yang lebih bervariasi, meskipun tetap menunjukkan potensi antibakteri alami. Penelitian ini diharapkan dapat memberikan dasar yang lebih kuat untuk pengendalian vibriosis di perikanan budidaya serta mendorong pengembangan alternatif antibakteri alami.

Pemanfaatan Limbah Biomassa Pertanian Menjadi Kompos Melalui Keterlibatan Masyarakat di Desa Pakuan Ratu, Way Kanan Buhani, Buhani; Kiswandono, Agung Abadi; Rinawati, Rinawati; Suharso, Suharso; Nurhasanah, Nurhasanah; Juliasih, Ni Luh Gede Ratna
Jurnal Pengabdian Kepada Masyarakat (JPKM) TABIKPUN Vol. 5 No. 1 (2024)
Publisher : Faculty of Mathematics and Natural Sciences - Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jpkmt.v5i1.122

Rendahnya keterlibatan masyarakat dalam pengelolaan sampah/limbah biomassa pertanian dan perkebunan menunjukkan perlunya upaya yang intensif dan berkelanjutan untuk meningkatkan partisipasi masyarakat dalam pengelolaan sampah di lingkungannya secara mandiri. Kegiatan Pengabdian kepada Masyarakat Unggulan (PKMU) telah dilaksanakan di Desa Pakuan Ratu, Kabupaten Way Kanan, bertujuan untuk mengubah dan meningkatkan paradigma masyarakat terhadap pengelolaan menjadi pupuk/kompos secara mandiri berbasis partisipasi aktif masyarakat. Potensialnya limbah biomassa pertanian dan perkebunan seperti cangkang buah kakao, cangkang buah kopi, dan serat kelapa untuk dijadikan pupuk organik yang berguna dalam meningkatkan kesuburan dan memperbaiki struktur tanah. Evaluasi menunjukkan bahwa program PKMU berhasil meningkatkan pemahaman masyarakat dalam mengenali, menyadari, dan mengimplementasikan pengolahan limbah biomassa.

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