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All Journal Science and Technology Indonesia Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Jurnal Pengabdian Pada Masyarakat Indonesia (JPPMI) Jurnal Pengabdian Masyarakat Panacea Jurnal Pengabdian Fakultas Pertanian Universitas Lampung
WAWAN ABDULLAH SETIAWAN, WAWAN ABDULLAH
Department of Biology, Faculty of Mathematics and Natural Sciences, Lampung University, Jalan Prof. Soemantri Brojonegoro No. 1 Bandar Lampung 35145, Indonesia
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Activity of Mangrove-Derived Fusarium equiseti 20CB07RF Extract Against Clinical, Antibacterial-Resistant Pseudomonas aeruginosa Bahri, Syaiful; Setiawan, Wawan Abdullah; Setiawan, Fendi; Lutfiah, Rosyidatul; Juliasih, Ni Luh Gede Ratna; Ambarwati, Yuli; Ahmadi, Peni; Arai, Masayoshi; Hendri, John; Hadi, Sutopo; Setiawan, Andi
Science and Technology Indonesia Vol. 9 No. 3 (2024): July
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2024.9.3.594-604

Abstract

Endophytic fungi originating from mangroves are potential sources of secondary metabolites with varying bioactivities. This research explores the bioactive metabolites produced by endophytes derived from mangrove plants. Endophytic fungi were collected from various parts of several mangrove plants (roots, stems, and leaves, as well as the surrounding mud). A total of 17 endophytics fungi were obtained. The isolates were derived from the leaves (1 isolate), stems (8 isolates), roots (5 isolates), and surrounding mud (3 isolates). A single fungal colony was cultured using solid-state fermentation for 14 days. The fermented fungal biomass was extracted using ethyl acetate (EtOAc) and evaluated for its antibacterial activity against clinical pathogenic bacteria. In the preliminary screening, the EtOAc extract of the CB07RF1 isolate exhibited notable growth-inhibitory effects against Pseudomonas aeruginosa. The isolate was verified by molecular identification using a study of the rDNA internal transcribed spacer (ITS) sequence, revealed that isolate CB07RF1 was very similar to Fusarium equiseti (99% similarity). Isolate 20CB07RF1, obtained by solid-state fermentation using a rice medium indicated as peptide compound group, and featured active components that exhibited potent growth inhibitory activity against Pseudomonas aeruginosa at a concentration of 12.5 mg/mL. This study demonstrates, for the first time, that Fusarium equiseti extracts grown in a rice medium contain antimicrobial compounds that can inhibit the growth of P. aeruginosa, an important clinical pathogen known for its antibacterial resistance. These findings accent mangrove endophytic fungi as important sources of bioactive compounds and will advance related research in the fields of biotechnology, pharmacology, and life sciences.
Novel Micrococcus unila to Produce Glucosamine by Solid-state Fermentation of Shrimp Shell Waste Setiawan, Wawan Abdullah; Setiawan, Andi; Salsabila, Nafila Khansa; Widyastuti, Widyastuti; Laila, Aspita; Juliasih, Ni Luh Gede Ratna; Irawan, Bambang; Ahmadi, Peni; Apriliana, Ety; Arai, Masayoshi; Hendri, John
Science and Technology Indonesia Vol. 9 No. 4 (2024): October
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2024.9.4.779-789

Abstract

This study aimed to assess glucosamine production through enzymatic activity, utilizing actinomycetes sourced from shrimp shell waste (SSW) in a solid-state fermentation (SSF) process. A total of 16 actinomycetes underwent chitinase activity screening, and the strain exhibiting the highest chitinolytic index was chosen for subsequent morphological and phylogenetic analyses. High Performance Liquid Chromatography (HPLC) was employed to analyze glucosamine produced from the bioconversion of SSW via SSF. Optimal conditions for glucosamine production were determined by varying time, pH, and temperature. Isolate 18D36-A2 showed the highest chitinolytic index of 1.02 in the 32-mm clean zone. Phylogenetic analysis revealed 97% similarity to the genus Micrococcus, identifying it as a novel Micrococcus unila strain 18D36-A2 and deposited in GenBank. This isolate effectively converted shrimp shells. The findings showcase the bioconversion of SSW to glucosamine through SSF using the Micrococcus unila 18D36-A2. Furthermore, this study establishes a foundation for future research on environmentally friendly and sustainable designs for glucosamine production.
Peningkatkan Pendapatan Kelompok Hutan Kemasyarakatan Kecamatan Pagar Dewa Lampung Barat Melalui Pemberian Stup Lebah Trigona Setiawan, Wawan Abdullah; Handayani, Kusuma; Hendri, John; Setiawan, Andi
Jurnal Pengabdian Masyarakat Panacea Vol 2, No 1 (2024): Jurnal Pengabdian Masyarakat Panacea
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jpmp.v2i1.12002

Abstract

Hutan Kemasyarakatan (HKm) termasuk hutan negara. Pemanfaatannya adalah untuk pemberdayaan masyarakat sekitar hutan. Untuk itu dalam pengelolaannya masyarakat harus dilibatkan agar tujuan dari pemberdayaan itu dapat tercapai. Peningkatan pendapatan menjadi tolak ukur untuk melihat kesejahteraan masyarakat, diantaranya adalah dengan pemberian stup (kotak) lebah Trigona (Apis trigona). Pemberian stup lebah ini bertujuan untuk membantu petani lebah di Hutan Kemasyarakatan Kecamatan Pagar Dewa Lampung Barat guna menambah pendapatannya. Hal itu disebabkan karena kurangnya stup lebah yang tersedia serta kurangnya sumber pakan menjadi hambatan dalam meningkatkan hasil panen madu oleh petani. Disini, artikel ini memberikan gambaran dan penjelasan tentang pelaksanaan program kerja Hutan Kemasyarakatan melalui pemberian stup lebah Trigona kepada Kelompok Tani Hutan (KTH) Wonorejo di Pekon Basungan, Lampung Barat. Pemberian stup lebah dilaksanakan menggunakan pendekatan kualitatif dimana modelnya adalah model naratif. Hasilnya, Kelompok Tani Hutan Wonorejo mendapatkan tambahan stup lebah dan tambahan sumber pakan untuk meningkatkan hasil panen madu. Artikel ini lebih lanjut diharapkan dapat menambah wawasan dalam bidang pertanian dan kehutanan khususnya petani untuk mengembangkan hasil hutan bukan kayu berupa pembudidayaan lebah madu. Kata Kunci: Hutan Kemasyarakatan, Peningkatan Pendapatan, Lebah Trigona, Hasil Hutan Bukan Kayu  Community Forests (Hutan Kemasyarakatan/HKm) are state forests. The use is for empowering communities around the forest. For this reason, in its management, the community must be involved so that the goals of empowerment can be achieved. Increasing income is a benchmark for assessing community welfare, including providing trigona bee stup. The aim of providing bee stup is to help bee farmers in the Pagar Dewa District Community Forest, West Lampung, to increase their income. This is because farmers are unable to increase their honey output due to a shortage of feed sources and bee stup. Here, this article provides an overview and explanation of the implementation of the Community Forest work program by providing Trigona bee stup to the Wonorejo Forest Farmers Group (Kelompok Tani Hutan/KTH) in Pekon Basungan, West Lampung. The provision of bee stup is carried out using a qualitative approach where the model is a narrative model. As a result, the Wonorejo Forest Farmers Group received additional bee stup and additional food sources to increase honey yields. It is intended that this essay will deepen readers' understanding of the domains of forestry and agriculture, particularly helping farmers create forest-based products that are not timber by raising honey bees.
Upaya Peningkatan Ketrampilan Ibu-ibu Dasawisma dengan Memanfaatkan Limbah Minyak Jelantah Handayani, Kusuma -; Setiawan, Wawan Abdullah; -, Mohamad Kanedi; Mumtazah, Dzul Fithria; Pratami, Gina Dania
Jurnal Pengabdian Pada Masyarakat Indonesia Vol 2 No 6 (2023): Desember : Jurnal Pengabdian Pada Masyarakat Indonesia
Publisher : Universitas Gajah Putih, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55542/jppmi.v2i6.821

Abstract

Minyak jelantah adalah minyak yang telah digunakan beberapa kali dalam proses pemanasan. Penggunaan minyak jelantah dalam waktu tertentu berdampak pada kesehatan akibat deposisi sel lemak yang terjadi di usus halus, pembuluh darah, jantung, dan hepar, apabila dibuang ke lingkungan akan menyebabkan pencemaran air tanah. Kegiatan Pengabdian ini bertujuan untuk melihat fenomena kepedulian masyarakat terhadap lingkungan dan dampak kesehatan jangka panjang sebagai implikasi dari pemanfaatan limbah minyak jelantah yang merupakan limbah anorganik rumah tangga. Sudah barang tentu setiap rumah tangga menghasilkan minyak jelantah karena penggunaan minyak goreng merupakan salah satu kebutuhan primer masyarakat. Dengan adanya perlakuan melalui pemanfaatan limbah minyak jelantah menjadi sabun, masyarakat memiliki peran penting terhadap pengendalian dari limbah tersebut. Hasil kegiatan ini menunjukan antusias yang cukup baik dari peserta, dilihat dari evaluasi selama proses kegiatan yang mengalami kenaikan hingga 87% dari sekian pertanyaan yang diajukan selama evaluasi proses.
Isolation and Identification of Fungi from Bactrocera dorsalis as Candidate Entomopathogenic Fungi Damayanti, Rizka Dwi; Emantis Rosa; Setiawan, Wawan Abdullah; Handayani, Tundjung Tripeni
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 11 No. 1 (2024)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v11i1.359

Abstract

Cultivation of horticultural crops especially fruits, has long been practiced in Indonesia. Citrus plants are one of the main fruit commodities developed in Indonesia. Increased citrus production is inseparable from challenges, especially in controlling harmful pest attacks. Bactrocera fruit fly has attracted worldwide attention because of its ability to attack more than a hundred horticultural crops and cause crop failure. Various ways have been done to control Bactrocera, such as protecting the fruit with wrapping and even the use of synthetic insecticides that cause many losses. To overcome this, innovations are needed using biological control. Entomopathogenic fungi are potential candidates for bioinsecticides. This study aims to isolate and identify entomopathogenic fungi from Bactrocera dorsalis from citrus plants. The entomopathogenic fungus isolation method uses the moist chamber method. Then the fungal isolates will be identified macroscopically and microscopically. The isolation results show that five types of fungi are identified from the body of Bactrocera dorsalis. The five fungi can be used as candidates for entomopathogenic fungi.
The Effectiveness of Nigrospora sp. and Penicillium sp. As Entomopathogenic Fungi Against in Bactrocera sp. Oktariana, Putri; Rosa, Emantis; Setiawan, Wawan Abdullah; Nurcahyani, Nuning
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 11 No. 1 (2024)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v11i1.361

Abstract

Pest attacks caused by fruit flies (Bactrocera sp.) can cause damage to fruit quality. Damage can become more severe due to secondary infection by bacteria or fungi which can cause fruit to rot. This research was conducted to determine the effectiveness and morphological changes caused by the fungus Nigrospora sp. and Penicillium sp. on the mortality of Bactrocera sp. This research was conducted using a Randomized Block Design (RBD) with two factors: the type of entomopathogenic fungi and the density of spores. Entomopathogenic fungi isolates were obtained from the isolation of entomopathogenic fungi from fruit flies (Bactrocera sp.) from previous studies. The spore density used for each fungus was 105, 106, and 107 spores/mL. Each treatment unit used 5 Bactrocera sp. adults with 3 repetitions. In the isolates of Nigrospora sp. they have resulted in mortality at a spore density of 105 spores/mL of 86%, a density of 106 spores/mL of 93%, and a density of 107 spores/mL of 100%. In the isolates of Penicillium sp. all treatments resulted in 100% mortality.
PENERAPAN PEMUPUKAN BERIMBANG UNTUK PENINGKATAN PRODUKSI KENTANG DI DESA TAMBAK JAYA, KECAMATAN WAY TENONG LAMPUNG BARAT Ardian, Ardian; Setiawan, Kukuh; Kamal, Muhammad; Hadi, Muhammad Syamsul; Yuliadi, Erwin; Yelli, Fitri; Sanjaya, Purba; Setiawan, Wawan Abdullah
Jurnal Pengabdian Fakultas Pertanian Universitas Lampung Vol. 2 No. 1 (2023): Jurnal Pengabdian Fakultas Pertanian Universitas Lampung Vol 2 No 1, Maret 2023
Publisher : Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jpfp.v2i1.7106

Abstract

Kentang merupakan salah satu tanaman utama yang ditanam oleh petani di Lampung Barat.  Hingga saat ini masalah utama adalah peningkatan kuaktas dan produksi kentang.  Oleh karena itu tujuan pengabdian ini adalah untuk mengedukasi petani mitra dalam meningkatkan produktivitas dan kualitas kentang per hektar melalui pemupukan berimbang sehingga pendapatan petani mitra dapat meningkat.  Sasaran penyuluhan berupa demonstrasi dan edukasi adalah petani Desa Tambak Jaya, Kecamatan Way Tenong, Kabupaten Lampung Barat. Ada tiga masalah yang menjadi fokus, yaitu ukuran ubi kentang yang tidak homogen, adanya pecah kulit ubi, serta ketahanan periode simpan yang tidak lama.  Target yang akan dicapai adalah peningkatan produksi dan kualitas kentang melalui sistem pemupukan berimbang. Produksi kentang yang dihasilkan dari kecamatan ini rata-rata mencapai 18-20 ton/ha. Produktivitas yang dihasilkan masih tergolong relatif rendah dibandingkan potensi hasil dari bibit yang ditanam, yaitu sekitar 40-50 ton/ha.  Salah satu penyebab rendahnya produktivitas kentang di wilayah ini karena petani tidak menerapkan pemupukan berimbang untuk produksi tinggi dan munculnya retak umbi dan rentannya pengelupasan kulit umbi. Kondisi ini menyebabkan produksi rendah dan kualitas umbi yang kurang.  Manfaat kegiatan pengabdian kepada masyarakat yaitu, petani binaan akan teredukasi dan memahami pentingnya pemupukan berimbang untuk peningkatan produksi dengan mutu yang prima, sehingga pendapatan petani meningkat dan adanya hubungan yang saling menguntungkan antara petani dan pengumpul/mitra usaha karena produk yang berkualitas. Hasil kegiatan pengabdian tim pengabdian dari Universitas Lampung membuat petani kentang di Tambak Jaya sangat tertarik dengan metode pemupukan berimbang dan sesuai dengan kebutuhan tanaman, sehingga produktivitas kentang mencapai 40 ton/ha dengan mutu umbi kentang premium
Co-Authors -, Mohamad Kanedi Ahmadi, Peni Ambarwati, Yuli Andi Setiawan Arai, Masayoshi Ardian Ardian Aspita Laila Damayanti, Rizka Dwi Emantis Rosa Erwin Yuliadi Ety Apriliana Fitri Yelli Gina Dania Pratami, Gina Dania Handayani, Kusuma - John Hendri Juliasih, Ni Luh Gede Ratna Kukuh Setiawan Kusuma Handayani, Kusuma Lutfiah, Rosyidatul Muhammad Kamal Muhammad Syamsul Hadi, Muhammad Syamsul Mumtazah, Dzul Fithria Nuning Nurcahyani Oktariana, Putri Rosa, Emantis Salsabila, Nafila Khansa Sanjaya, Purba Setiawan, Fendi Sutopo Hadi SYAIFUL BAHRI Tengku Riza Zarzani N Tundjung Tripeni Handayani, Tundjung Tripeni Widyastuti Widyastuti