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Mouthwash Potential of Ethanol Extract of Ajwa Date Seeds (Phoenix dactylifera L.) against Porphyromonas gingivalis Bacteria Mauriska Nada Silmi Almisky; Etny Dyah Harniati; Steffi Triany Arnov; Ariyani Faizah
Journal of Indonesian Dental Association Vol. 9 No. 1 (2026): April
Publisher : Indonesian Dental Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32793/jida.v9i1.1535

Abstract

Introduction: Periodontal disease is a major oral health problem worldwide, with Porphyromonas gingivalis recognized as one of the primary causative bacteria. Ajwa date seeds (Phoenix dactylifera L.) have potential for development as a natural mouthwash due to their antibaterial properties, which are attributed to various bioactive compounds, including flavonoids, tannins, and saponins. Objective: To evaluate the antibacterial effect of a mouthwash containing ethanol extract of ajwa date seeds (Phoenix dactylifera L.) against the growth of Porphyromonas gingivalis. Methods: This laboratory experimental study employed a post-test-only control group design. The samples were divided into five groups: a positive control, a negative control, and three treatment groups receiving mouthwash containing ethanol extract of ajwa date seeds at concentrations of 3.25%, 6.5%, and 12.5%. Antibacterial activity was evaluated using the disc diffusion method on Mueller–Hinton Agar (MHA), with six replicates for each group. Data were analyzed using one-way ANOVA followed by Tukey's Honestly Significant Difference (HSD) post hoc test. Results: Mouthwash containing ethanol extract of ajwa date seeds inhibited the growth of Porphyromonas gingivalis, as demonstrated by the formation of inhibition zones around the discs. A concentration-dependent increase in the diameter of the inhibition zone was observed. The mean inhibition zone diameters were 10.175 mm, 12.300 mm, and 14.442 mm for the 3.25%, 6.5%, and 12.5% extract concentrations. Conclusion: Mouthwash containing ethanol extract of ajwa date seeds (Phoenix dactylifera L.) significantly inhibited the growth of Porphyromonas gingivalis. The antibacterial activity in 12.5% concentration demonstrating the greatest inhibitory effect.  
Comparative Analysis of Patient Safety Culture Among Trained and Untrained Staff at the Educational Dental and Oral Hospital in Semarang: Cross-Sectional Study Retno Kusniati; Etny Dyah Harniati; Yasinia Annisa Purbomurti
Media Publikasi Promosi Kesehatan Indonesia Vol. 8 No. 7 (2025)
Publisher : Fakultas Kesehatan Masyarakat, Universitas Muhammadiyah Palu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56338/mppki.v8i7.7246

Abstract

Introduction: Patient safety (PS) is crucial to healthcare services, including in dental practice. A good patient safety culture can reduce adverse events and improve the quality of care. Patient safety training is one strategy to improve safety culture. However, there are still gaps in the implementation of patient safety culture (PSC). This study aims to analyze the differences in patient safety culture between staff who have received patient safety training and those who have not. Methods: This study was cross-sectional using the Hospital Survey on Patient Safety Culture (HSOPSC) questionnaire. Participants were staff at the Dental and Oral Hospital of the University of Muhammadiyah Semarang who met the inclusion criteria. Data were collected for two weeks, and statistical analysis was performed using the Mann-Whitney test to compare differences in patient safety culture between groups that had received training and those that had not. Results: Of the 12 dimensions of patient safety culture, teamwork within the unit (89.6 %) and organizational learning–continuous improvement (85.8%) had the highest positive response rates. In contrast, the dimensions of staffing (35.4 % ) and openness of communication (36.2%) recorded the lowest positive responses. Staff who had attended patient safety training showed a higher positive response than those who had not, but the results of the difference in mean scores with 95% confidence intervals (CI) proved to be statistically insignificant (p = 0.563). Conclusion: Patient safety training increased the perception of safety culture, although there was no significant difference in scores between staff who had and had not attended training. A supportive work environment and good teamwork are essential in shaping a patient safety culture. Hospitals should consider strategies to increase staffing so that they are not working in “crisis mode” to maintain patient safety and to support the Indonesian Ministry of Health and WHO Patient Safety Action Plan.
Antibacterial Potential of Ajwa Seed (Phoenix dactylifera L.) Ethanol Extract Mouthwash Preparation Against Porphyromonas gingivalis Etny Dyah Harniati; Retno Kusniati; Siti Aminah; Yasinia Annisa Prubomurti; Amelia Dwi Rustanti
Jurnal Kesehatan Gigi Vol. 13 No. 1 (2026): June 2026
Publisher : Jurusan Kesehatan Gigi, Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jkg.v13i1.13043

Abstract

Chronic periodontitis, primarily caused by Porphyromonas gingivalis, often requires adjunctive mouthwash therapy. While Chlorhexidine is the gold standard, its long-term use leads to various side effects. Date seeds, containing active compounds like polyphenols, flavonoids, and tannins, offer potential as a natural, environmentally friendly antibacterial alternative against Porphyromonas gingivalis. Research Objectives: This study aimed to develop and evaluate a date seed extract-based mouthwash for its effectiveness against Porphyromonas gingivalis growth. The experimental design tested three extract concentrations (5%, 7.5%, and 10%) for antibacterial activity. Phytochemical screening identified active compounds, and antibacterial testing was conducted using the agar diffusion method. showed date seed extract contains secondary metabolites such as alkaloids, flavonoids, tannins, and steroids, indicating antibacterial potential. The formulated mouthwash had a liquid, brownish texture with a characteristic aroma, though it produced undissolved precipitates. Importantly, antibacterial testing demonstrated significant inhibitory capability against Porphyromonas gingivalis, evidenced by clear inhibition zones, with a significant difference (p < 0.001) in diameter across concentrations. In conclusion, Ajwa date seeds effectively inhibit Porphyromonas gingivalis growth. A higher concentration of date seed extract in the mouthwash formula results in greater inhibitory power