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All Journal HAYATI Journal of Biosciences Jurnal Penelitian dan Pengembangan Pendidikan Bioedukasi: Jurnal Pendidikan Biologi Jurnal Terapan Abdimas Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Bioedukatika Biosfer: Jurnal Pendidikan Biologi Diklabio: Jurnal Pendidikan dan Pembelajaran Biologi Journal of Educational Research and Evaluation WIDYA LAKSANA JURNAL BIOEDUCATION Best Journal (Biology Education, Sains and Technology) Jurnal Kependidikan: Jurnal Hasil Penelitian dan Kajian Kepustakaan di Bidang Pendidikan, Pengajaran dan Pembelajaran Bioscientist : Jurnal Ilmiah Biologi BIOMA : Jurnal Biologi dan Pembelajarannya Biodik: Jurnal Ilmiah Pendidikan Biologi
Sunandar, Ari
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Physics Public Health Social Sciences Other

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Journal : HAYATI Journal of Biosciences

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Variation of Musa spp. in West Kalimantan, Indonesia, Based on rbcL Chloroplast DNA Sunandar, Ari; Fajri, Hayatul; Qurbaniah, Mahwar
HAYATI Journal of Biosciences Vol. 31 No. 5 (2024): September 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.5.942-950

Abstract

West Kalimantan is home to Musa spp. conservation and genetic assessment of wild banana relatives are important for future breeding purposes. The present study aims to evaluate the genetic relationship of Musa spp. in West Kalimantan by analyzing the rbcL chloroplast DNA using phylogenetic analysis. The methods in this study were sampling, DNA extraction, PCR of rbcL fragment, and data analysis. The specific primer was used to amplify the rbcL chloroplast DNA of ten accessions of Musa spp. in West Kalimantan. The results showed that the area of the rbcL region of Musa spp. in this study was estimated at 587-591 bp. It showed high variability with a conservation level A+T content of 56.95%. The rbcL sequences of Musa spp. have polymorphic sites on 13 numbers of nucleotides. The phylogenetic analysis with an ML algorithm of 35 Musa spp. from West Kalimantan and GenBank data was successfully divided into 4 main clades, and the bootstrap value was 80-81%. This study is expected beneficial for taxonomic, conservation, and banana breeding efforts.
Anti-glucosidase Activity and Antioxidant Capacity of Premna serratifolia from Sintang, Kapuas Hulu, and Sambas District, West Kalimantan, Indonesia Hadiarti, Dini; Sunandar, Ari; Cipta, Indra; Kurniawan, Rizmahardian Ashari
HAYATI Journal of Biosciences Vol. 33 No. 1 (2026): January 2026
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.33.1.153-166

Abstract

The secondary metabolites in plants such as Premna serratifolia (P. serratifolia) are influenced by their growing locations, which in turn affects their bioactivity. This study aimed to compare the antioxidant activity and α-glucosidase inhibition of P. serratifolia leaf extracts from the Sintang, Kapuas Hulu, and Sambas districts in West Kalimantan. Antioxidant capacity was evaluated using the DPPH method, while the reduction of α-glucosidase activity was assessed using the PNPG method. Functional groups and active compounds were identified using FTIR and UHPLC-Q-Orbitrap HRMS. The findings indicated that the extract from Kapuas Hulu, obtained through maceration (KHM), exhibited the highest antioxidant activity (IC₅₀ 18.39) and contained the most total phenolic content (TPC). The best α-glucosidase inhibition activity (IC₅₀ 4194.14) was found in the extract from Sambas obtained through Soxhlet extraction (SSI), which had the highest total flavonoid content (TFC). Principal Component Analysis (PCA) revealed that the functional groups and active compounds of P. serratifolia from Kapuas Hulu and Sintang were similar, as both regions are located in eastern West Kalimantan. Additionally, Partial Least Squares (PLS) analysis revealed that the C-H alkane, O-H, and isoferulic acid were the most influential compounds in determining antioxidant activity. At the same time, the C=O group and NP-000308 predominantly influenced α-glucosidase inhibition.
Co-Authors Adriana, Adriani Agustini Agustini, Agustini Airlangga, Liyu Vani Aji Anandita Eka Setiadi Anisyah Yuniarti Apriani, Resti Cipta, Indra Dini Hadiarti Fajri, Hayatul Fiqih, Febriana Nur Hidayah, Wulan Hardianti Kabani, Rana Karnina, Wulan Krisdayanti, Krisdayanti lena, Serly Marsa Mariska, Sonia Nengsih, Nengsih Ningsih, Sri Hadi Prawati, Lisa Qurbaniah, Mahwar Rahayu, Hanum Mukti Rahmah, Adnin RINI RINI Ririn, Asma Rizmahardian Ashari Kurniawan Rusiana, Maya Sabilla, Sabilla Narulita Sari, Wita Kusuma Setiandi, Anandita Eka Sofianti, Mery Sumarni, Elis Tania, Rahmayana Dwi WOLLY CANDRAMILA Wulandari, Selvi