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All Journal Journal of Food and Pharmaceutical Science JURNAL TEKNOLOGI LINGKUNGAN Indonesian Biodiversity Journal Balobe: Jurnal Pengabdian Masyarakat
Manurung, Brian Saputra
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Public Health Social Sciences Veterinary Other

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Enzymes Involving in Chocolate Processing Kiyat, Warsono El; Monica, Audrey; Qomariyah, Noor; Manurung, Brian Saputra
Journal of Food and Pharmaceutical Sciences Vol 6, No 1 (2018): J. Food Pharm. Sci (January-April)
Publisher : Fakultas Farmasi, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (369.988 KB) | DOI: 10.14499/jfps

Abstract

Indonesia is one of the largest cocoa-producing countries in the world, but the productivity of Indonesian cocoa is relatively low compared to other cocoa-producing countries. Many cocoa beans are exported in raw form (raw cocoa beans) so they do not have high economic value. In addition, cocoa beans also can not be consumed directly and must be processed first. The processing of cocoa beans includes curing stage, breaking the fruit, fermentation, soaking and washing, drying, sorting, and storage to producing cacao almonds. The fermenting process of cocoa beans involves the role of the polyphenol oxidase to produce flavor precursor compounds. Furthermore, to improve the quality of the resulting chocolate, protease and carboxypeptidase can be used in the processing of cacao almonds to enhance flavor precursor compounds forming to produce chocolate with stronger flavor characteristics. Processed chocolates can be developed into confectionery with a soft or liquid filler by utilizing invertase to obtain a solid filler during processing that may turn into liquid during storage. Keywords : cocoa, chocolate, enzyme, processing.
Efektivitas Pengolahan Air yang Mengandung Amonia Konsentrasi Tinggi Menggunakan Konsorsium Probiotik Komersial dan Bakteri Sedimen Kolam Lele Ambarsari, Hanies; Syah, Iman; Nugroho, Rudi; Manurung, Brian Saputra; Suciati, Fuzi
Jurnal Teknologi Lingkungan Vol. 21 No. 1 (2020)
Publisher : Center for Environmental Technology - Agency for Assessment and Application of Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1065.099 KB) | DOI: 10.29122/jtl.v21i1.3478

Abstract

ABSTRACTNitrification is one of the most widely used methods to reduce ammonia concentration in wastewater.The purpose of this study was to determine the effectiveness of high-concentrated ammonia nitrification by the consortium between commercial probiotics and bacteria from the sediment of the catfish pond. The study was conducted on batch system bioreactors with a working volume of 1 liter containing 100 mg/L ammonia solution and 50 grams sediment of catfish pond. This study used a treatment variation of the concentration of commercial probiotics and the bacterial isolate from the catfish pond sediment using glucose as the carbon source. The variations of commercial probiotics added to the bioreactor were 5 ml/L, 10 ml/L and 15 ml/L. The variations of glucose concentration were 0 g/L and 3,9 g/L. Analysis of ammonia concentration was carried out by spectrophotometry using the phenate method. The highest removal efficiency of ammonia was 92.35% in the bioreactor with a mixture of 15 ml/L commercial probiotics and 3.9 g/L glucose with the fastest ammonia rate was on the third day of the experimental period. The addition of glucose in the bioreactors could increase ammonia removal by 57.39%. The result of statistical analysis indicated that variations in the concentration of commercial probiotic indicated no statistically significant difference in ammonia removal (P> 0.05), while variations in glucose concentration showed a statistically significant difference in ammonia removal (P <0.05). Three isolates were successfully isolated on specific media for nitrifying bacteria. The result of bacterial identification showed that three isolated bacteria were Bacillus sp., Aeromonas salmonicida, and Burkholderia cepacia.Keywords: ammonia, nitrification, sediment of catfish pond, commercial probioticABSTRAKNitrifikasi merupakan salah satu metode yang paling banyak digunakan untuk mengurangi konsentrasi amonia pada limbah cair. Penelitian ini bertujuan untuk mengetahui efektivitas penambahan sedimen kolam lele dan probiotik komersial dalam nitrifikasi amonia konsentrasi tinggi. Penelitian menggunakan bioreaktor sistem batch dengan volume kerja 1 liter yang berisi larutan amonia 100 mg/L dan 50 gram sedimen kolam lele. Perlakuan pada penelitian ini terdiri dari pemberian variasi konsentrasi probiotik komersial dan sedimen kolam lele dengan sumber karbon glukosa. Variasi probiotik komersial yang ditambahkan pada bioreaktor adalah 5 ml/L, 10 ml/L dan 15 ml/L sedangkan variasi konsentrasi glukosa adalah 0 g/L dan 3,9 g/L. Analisis konsentrasi amonia dilakukan secara spektrofotometri menggunakan Metode Fenat. Hasil penurunan rata-rata amonia tertinggi adalah sebesar 92,35% pada bioreaktor dengan campuran probiotik komersial 15 ml/L dan glukosa 3,9 g/L dengan laju penurunan ammonia tercepat pada hari ke-3 periode eksperimen. Penambahan glukosa pada bioreaktor mampu meningkatkan penurunan amonia sebesar 57,39%. Analisis statistik menunjukkan bahwa variasi konsentrasi probiotik komersial tidak menunjukkan perbedaan signifikan pada penurunan amonia (p>0,05) sedangkan variasi konsentrasi glukosa menunjukkan perbedaan yang signifikan terhadap penurunan amonia (p<0,05). Tiga isolat berhasil diisolasi menggunakan media spesifik bakteri nitrifikasi. Hasil identifikasi dari tiga bakteri menunjukkan bahwa isolat tersebut adalah Bacillus sp., Aeromonas salmonicida dan Burkholderia cepacia.Kata kunci: amonia, nitrifikasi, sedimen kolam lele, probiotik komersial
MENGENAL SISI LAIN POHON MAYANG BAGI KEHIDUPAN MANUSIA Kaihena, Martha; Nusaly, Windy Natalia; Patty, Kristi Lenci; Salamena, Fuadiska; Manurung, Brian Saputra
Balobe: Jurnal Pengabdian Masyarakat Vol 3 No 2 (2024): Balobe: Jurnal Pengabdian Masyarakat
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/balobe.3.2.113-117

Abstract

The community service activity with the title "Knowing the Other Side of Mayang Tree for Human Life" is a form of concern for academics in the field of Biology for the spread of society and the development of home industry products that have an impact on improving the health and economy of the community. The transfer of information in the form of processing techniques, ways of utilisation, manufacturing procedures, and marketing techniques is carried out to educate the community in order to increase knowledge about the products and its biological processes. Scientifically, in the procedure of utilisation and processing of palm fruit (mayang), there are many science studies found in everyday life. In addition, indirectly, community students as recipients of information can apply it and become home industry producers. This is very helpful for the community in the utilisation of local wisdom in the area considering kolang kaling is a by-product of palm fruit (mayang) processing.
Analysis Of The Potential Of Natural Sterol Compounds From Tin (Ficus carica) Leaves as Anti-Hypercholesterolemia with In Silico Tests Taihuttu, Monalisa Pertiwi Jeriska; Manurung, Brian Saputra; Simal, Rufiati; Watung, Fernando A.
Indonesian Biodiversity Journal Vol. 6 No. 1 (2025): April 2025 (In Progress)
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v6i1.11961

Abstract

This study aims to determine the potential of natural sterol compounds from Tin (Ficus carica) leaves as anticholesterolemic using in silico test. This study employed an in silico approach using molecular docking methods to evaluate the potential of sterol compounds as anti-hypercholesterolemic agents, in comparison to other references compounds. Data processing and interpretation were conducted using molecular databases for both ligands and target proteins. The analysis utilized several computational tools, including PyRx 0.8, PyMOL, LigPlus, and Discovery Studio 2016 Client. The compounds used in this research  were sterol, fluvastatin and simvastatin (as control), with HMG CoA reductase as the target protein. The results showed that the highest binding affinity value was fluvastatin which is -8.3 kcal/mol. Sterol compounds are compounds with lower binding affinity which is -7.7 kcal/mol. From the visualization results, it is known that the binding distance between sterol compounds with target proteins (HMG CoA reductase) is between 2.69 to 5.49 Å, and the binding distance between simvastatin compounds with target proteins (HMG CoA reductase) is between 2.76 to 5.36 Å, and the binding distance between fluvastatin compounds with target proteins (HMG CoA reductase) is between 1.95 to 5.26 Armstrong. While based on the comparison of the binding side of strerol, simvastatin and fluvastatin, it is known that the three compounds have the same site because they bind to the same amino acid residues, namely ARG (B: 515), TYR (A: 533), TYR (B: 533), TYR (B: 517), PRO (A: 511). The results of this research indicate that the natural sterol compounds found in Tin (Ficus carica) leaves have potential as anti-hypercholesterolemic agents, based on reverse docking analysis. These sterol compounds bind to the same active site as the control drugs, simvastatin and fluvastatin, and exhibit comparable binding affinity values
Co-Authors Hanies Ambarsari Kiyat, Warsono El Martha Kaihena Monica, Audrey Nusaly, Windy Natalia Patty, Kristi Lenci Qomariyah, Noor Rudi Nugroho Salamena, Fuadiska Simal, Rufiati Suciati, Fuzi Syah, Iman Taihuttu, Monalisa Pertiwi Jeriska Watung, Fernando A.