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All Journal Meditory : The Journal of Medical Laboratory Journal of Tropical Crop Science Biopendix: Jurnal Biologi, Pendidikan dan Terapan Rumphius Pattimura Biological Journal Balobe: Jurnal Pengabdian Masyarakat PENGAMATAN: Jurnal Pengabdian Masyarakat untuk Ilmu MIPA dan Terapannya
Mose, Windi
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Mathematics Social Sciences Other

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Journal : Rumphius Pattimura Biological Journal

 1 
Potential of Hotong Flour (Setaria italica (L.) Beauv) from Buru Island in Reducing Blood Cholesterol Levels in Wistar Rats with Diabetes Mellitus Mose, Windi; Wael, Syahran; Salmawati, Mimi; Wahyudi, Didik; Astuti, Pudji
RUMPHIUS Vol 6 No 1 (2024): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv6i1p001-005

Abstract

Hotong flour (Setaria italica) can be used as a functional food as therapy in the treatment of diabetes mellitus by reduce cholesterol levels in the blood. Hotong is a rice-like food crop that is usually consumed by the people of Buru Island. Hotong seeds have quite high nutritional content, namely containing 11.18% protein, 2.36% fat, 73.36% carbohydrates, 11.78% water, and 1.32% ash. The energy produced per 100 grams of hotong seeds is 359 calories. Hotong plants can be used as an alternative commodity in a carbohydrate-producing food diversification program. This study aims to determine the potential dose of hotong flour in treating diabetes mellitus by reducing cholesterol levels in the blood of rat. This research used 24 wistar rats animals which were divided into 6 groups which were given hotong flour for 21 days and the cholesterol levels were evaluated on the 7th, 14th, and 21st day. Determination of rat blood cholesterol levels using the enzymatic method. Hotong flour can reduce blood sugar levels at doses of 1 g/kg bw and 1.5 g/kg bw in rat that have been made diabetic by examination on days 7, 14 and 21 days.
OPTIMIZATION OF PROTOPLAST ISOLATION FROM DIFFERENT TYPES OF SAMPLES Mose, Windi; Wael, Syahran
RUMPHIUS Vol 6 No 2 (2024): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv6i2p056-061

Abstract

Isolation of protoplasts in several plant species still requires optimization to produce protoplasts that are viable and can be used for further analysis. This research aims to optimize protoplast isolation protocols in several plants: Orchid Dendrobium macrocarpus, potato Solanum tuberosum, and microalgae Phaeodactylum tricornutum, Botryococcus braunii, and Spirulina sp. The results showed that in vitro explant selection had higher sample uniformity, and a combination of enzyme solutions could be used to increase the effectiveness of protoplast isolation on D. macrocarpus, S. tuberosum, and Spirulina sp. This study provides information about protoplast isolation techniques and testing their viability as an attempt for plant breeding through micropropagation.
IDENTIFICATION AND DENSITY OF MOSQUITO SPECIES THAT CAUSE FILARIASIS DISEASE IN TANIWEL WEST SERAM DISTRICT Senen, Yan Berlin; Mose, Windi; Rehena, Johanis Fritzgal; Wael, Syahran
RUMPHIUS Vol 5 No 1 (2023): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv5i1p001-006

Abstract

The purpose of this study was to look at the types and density of mosquitoes in the Taniwel district. Research time is August 2021. This research is descriptive in nature. where only see the type and density using the existing formula. In this study, Mansonia and Anopheles mosquitoes were found. The results showed that mosquitoes found in East Taniwel sub-district, Sohuwe Village, Lumahlatal, and Maloang found 2 genera of mosquitoes namely Mansonia and Anopheles with mansonia density in Sohuwe village 3.29%, Lumahlatan 2.83% and Maloang 2.66. while the density of Anopheles in Sohuwe village was 3.62%, Lumahlatan 3.45% and Maloang 2.66%.
ANALYSIS OF THE HEAVY METAL CHROMIUM (Cr) IN MULLETS (Mugil dussumieri) SPECIES IN THE WAI RUHU GALALA RIVER AMBON CITY Wurlianty, Benjamina; Mose, Windi; Wael, Syahran
RUMPHIUS Vol 5 No 1 (2023): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv5i1p012-017

Abstract

The heavy metal chromium (Cr) is one of the heavy metals which is toxic and h armful if it accumulates in the body of organisms at high concentrations. Heavy metals can accumulate through the food chain, namely the higher the level of the food chain occupied by an organism, the accumulation of heavy metals in the body also increases. The aim of the research was to determine the liver contamination of mullet exposed to chromanum metal. The method used in this study was the purposive sampling method. Sediment and mullet at the Basic Kimia FKIP Laboratory while to measure the content of heavy metal Cr in sediment and mullet at the Karpan Health Laboratory, Ambon 11 Desember 2022 - 18 Januari 2023 using the AAS (Atomic Absorption Spectrophotometer). The Cr content in the sediment and mullet obtained at the estuary of the Wai Ruhu Galala river, Ambon City is still below a predetermined threshold. The research results obtained from the content of heavy metal cr in the sediment which is equal to 9,2888 Ppb while in mullet it is equal to 5,8185 Ppb. The presence of heavy metal cr is caused by contaminants which may originate from community activities around this location in the form of housedold waste coated with cr metal discharged directly into the water body such as household appliaces coated with cr metal and cans paint.
Measurement of Nitrate Reductase Activity in Vivo Using Sepctrophotometry Method Mose, Windi; Dorlandt, Mauritius; Riupassa, Pieter Agusthinus; Wael, Syahran
RUMPHIUS Vol 7 No 1 (2025): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv7i1p025-030

Abstract

Spectrophotometry is a quantitative measurement technique of a compound based on the measurement of absorbance or light transmission passing through the compound. The spectrophotometric method can be used to analyze the content of compounds. One of the enzymes whose activity can be measured in vivo is the nitrate reductase enzyme. The nitrate reductase enzyme is an enzyme that plays a role in the synthesis of ammonium and amino acids. The results of measuring the activity of the nitrite reductase enzyme with spectrophotometry showed that the highest NRA value was found in the leaves of red chili plants and the lowest was in the sugarcane leaf sample. NRA can be used as a selection parameter to predict the results of a plant, and has the potential to be applied to seed plants.
Co-Authors Apituley, Edwin T. Baszary, Chomsa Dintasari Umi Didik Wahyudi Dorlandt, Mauritius Fredy Leiwakabessy Iskandar, Hayati Minarsih LAURY CHARA HUWAE Manurung, Brian Saputra Mimi Salmawati Nusaly, Windy Natalia Pambudi, Jembar Patty, Kristi Lenci Pieter Agusthinus Riupassa PUDJI ASTUTI PUTRANTO, Riza Arief Putri Usman, Dwi Anggara Rehena, Johanis Fritzgal Salamena, Fuadiska Salmawati, Mimi Senen, Yan Berlin Suhandono, Sonny Sustiprijatno Sustiprijatno, Sustiprijatno Syahran Wael TATI KRISTIANTI Theopilus W Watuguly Wurlianty, Benjamina