Garuda - Garba Rujukan Digital

Aromatase gene expression and masculinization of Nile tilapia immersed in water 36 °C containing 17α-methyltestosterone Fauzan, Agung Luthfi; Soelistyowati, Dinar Tri; Junior, Muhammad Zairin; Hardiantho, Dian; Setiawati, Mia; Alimuddin, ,
Jurnal Akuakultur Indonesia Vol. 16 No. 1 (2017): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT Immersion of undifferentiated larval tilapia in high temperature and 17α-methyltestosterone (MT) cab increase the male ratio. However, the effectiveness of immersion in high temperature of water containing MT remains to be evaluated. The purposes of this study were: 1) evaluate the male ratio, growth, and survival of tilapia, and 2) analyze the aromatase brain-type gene expression level in tilapia after immersing in high temperature (36 °C) containing MT at 2 mg/L for four hour with single and double immersion. Aromatase gene expression was analyzed by semi-quantitative RT-PCR (sqRT-PCR) method. The result showed that higher monosex male ratio was obtained by single immersion of MT at 36 °C at room temperature. Gene expression level of aromatase brain-type was lower on single immersion and increased significantly at second immersion compared to control (immersion at room temperature without MT). Immersion using MT and high temperature had no significant effect on fish survival. However the specific growth rate and fish biomass were higher than control. Thus, monosex male tilapia can be produced by single immersion of undifferentiated larvae at 36 °C temperature containing MT. Keywords: male ratio, aromatase, Oreochromis niloticus, temperature, 17α-methyltestosterone ABSTRAK Perendaman larva ikan nila yang belum terdeferensiasi kelaminnya dengan suhu tinggi dan hormon 17α-metiltestosteron (MT) dapat meningkatkan nisbah kelamin jantan. Tetapi, efektivitas perendaman menggunakan MT pada suhu tinggi belum diteliti. Tujuan dari penelitian ini adalah 1) mengevaluasi nisbah kelamin jantan, pertumbuhan, dan kelangsungan hidup ikan nila, dan 2) menganalisis ekspresi gen aromatase tipe-otak pada ikan direndam menggunakan MT dengan dosis 2 mg/L selama empat jam sebanyak satu dan dua kali perendaman pada suhu 36 °C. Ekspresi gen aromatase dianalisis menggunakan metode RT-PCR semi-kuantitatif (sqRT-PCR). Hasil penelitian menunjukkan bahwa kombinasi perendaman MT satu kali pada suhu 36 °C lebih tinggi menghasilkan ikan nila jantan monoseks dibandingkan perendaman MT satu kali pada suhu ruang. Tingkat ekspresi gen aromatase tipe otak pada perendaman satu kali lebih rendah, dan meningkat secara signifikan pada perendaman kedua dibandingkan dengan kontrol (perendaman pada suhu ruang tanpa MT). Perendaman larva menggunakan MT dan suhu 36 °C tidak berpengaruh nyata terhadap kelangsungan hidup, tetapi laju pertumbuhan spesifik dan biomassa ikan perlakuan tersebut lebih tinggi daripada kontrol. Dengan demikian, ikan nila jantan monoseks dapat diproduksi dengan perendaman satu kali pada larva yang belum terdeferensiasi jenis kelaminnya menggunakan MT pada suhu 36 °C. Kata kunci: rasio jantan, aromatase, Oreochromis niloticus, suhu, 17α-metiltestosteron

Financial Viability Assessment of Koi (Cyprinus Rubrofuscus) Culture Through Selective Breeding Programs : A Case Study of Indonesian Commercial Koi Farms Fauzan, Agung Luthfi; Ramadhana, Ahnadia Wulan; Dewi, Nina Nurmalia
Jurnal Media Akuakultur Indonesia Vol 5 No 2 (2025): Indonesian Journal of Aquaculture Medium
Publisher : Program Studi Budidaya Perairan Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/mediaakuakultur.v5i2.7332

This study aims to evaluate the financial performance of the koi fish farming industry (Cyprinus rubrofuscus) in Indonesia through a selective breeding program. Data collected between 2021 and 2024, covering business development, operational costs, investment costs, and capital estimates, was obtained from 24 koi fish farming businesses in the Java region. Businesses using genetic screening aim to reduce financial disparities, which is different from traditional aquaculture methods. The analysis's results indicate that the implementation of the selective breeding program in a scientific manner yielded a higher profit margin of 34.2% than the traditional method, which only reached 18.7%. Although the initial investment is more substantial (about Rp28,5 million per hectare), the time frame required to pay for the investment is more efficient, i.e., between 18 and 24 months. The price of koi fish is higher than the market price, possibly reaching 2.8% of the price of koi fish, with the selling price per tail ranging from Rp450,000 to Rp1,200,000. In addition, there was a 15% increase in feed efficiency and a 3% decrease in mortality, which means a 7.8% reduction. Thus, this selective breeding program can improve koi farming production and sales performance by applying genetic technology, which can help overcome the financial crisis in the aquaculture sector in developing countries.

SISTEM EVALUASI DAN MANAJEMEN PRODUKSI BENIH IKAN KOI (Cyprinus carpio) DI OMAH KOI FARM INDONESIA Fauzan, Agung Luthfi; Effendi, Irzal
OCTOPUS: JURNAL ILMU PERIKANAN Vol. 12 No. 2 (2023): OCTOPUS
Publisher : Universitas Muhammadiyah Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26618/o.v12i2.14875

Ikan koi (Cyprinus carpio) merupakan salah satu komoditas ikan hias air tawar yang memiliki potensi ekonomis penting, baik secara nasional maupun international. Pembenihan merupakan suatu kegiatan dalam budidaya untuk menghasilkan benih yang sangat menentukan pada tahapan kegiatan budidaya selanjutnya. Tujuan dari penelitian ini adalah mengevaluasi sistem dan pengelolaan produksi benih ikan koi di Omah Koi Farm Indonesia. Parameter uji yang diukur yaitu fekunditas, fertilization rate (FR), hatching rate (HR), kelangsungan hidup benih, kualitas benih, dan kualitas air. Proses pembenihan meliputi persiapan kolam, seleksi induk, pemijahan, penetasan telur, pemeliharaan larva, pemanenan larva, penebaran larva, pemberian pakan, pemberian pakan benih, pemanenan benih, seleksi benih, dan pengelolaan kualitas air. Hasil penghitungan fekunditas Kohaku dan Showa masing-masing 30.000 butir dan 35.000 butir. Nilai FR yang didapatkan yaitu 90,71% dan HR sebesar 82,93%. Rata-rata kelangsungan hidup benih koi umur 45 hari 91,75% dengan rata-rata benih grade high quality (HQ) 150 ekor, grade A sebanyak 450 ekor, dan grade B sebanyak 450 ekor. Kisaran suhu pada kolam pemeliharaan larva 25-27 0C, pH air berkisar 7,9-8,5, DO berkisar 5,0-6,0, dan amonia sebesar 0,01.

Evaluation and Management System for Koi Fish (Cyprinus carpio) Fingerlings Production at Omah Koi Farm Indonesia Fauzan, Agung Luthfi; Effendi, Irzal
Jurnal Media Akuakultur Indonesia Vol 3 No 4 (2023): Indonesian Journal of Aquaculture Medium
Publisher : Program Studi Budidaya Perairan Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/mediaakuakultur.v3i4.3636

Koi fish (Cyprinus carpio) is a freshwater ornamental fish commodity that has important economic potential, both nationally and internationally. Breeding is an activity in cultivation to produce seeds which are very decisive at the next stage of cultivation activities. The purpose of this study was to evaluate the system and management for koi fish seed production at Omah Koi Farm Indonesia. The test parameters measured were fecundity, fertilization rate (FR), hatching rate (HR), seed survival, seed quality, and water quality. The hatchery process includes pond preparation, parent selection, spawning, hatching eggs, rearing larvae, harvesting larvae, stocking larvae, feeding, feeding seeds, harvesting seeds, selecting seeds, and managing water quality. The results of the Kohaku and Showa fecundity calculations were 30,000 and 35,000 items respectively. The FR value obtained is 90,71% and HR is 82,93%. The average survival of koi seeds aged 45 days was 91,75% with an average of 150 high quality (HQ) seeds, 450 grade A seeds, and 450 grade B seeds. The temperature range in the larval rearing ponds was 25-27 0C, the water pH ranged from 7.9-8.5, DO ranged from 5.0-6.0, and ammonia was 0.01.

Financial Viability Assessment of Koi (Cyprinus Rubrofuscus) Culture Through Selective Breeding Programs : A Case Study of Indonesian Commercial Koi Farms Fauzan, Agung Luthfi; Ramadhana, Ahnadia Wulan; Dewi, Nina Nurmalia
Jurnal Media Akuakultur Indonesia Vol 5 No 2 (2025): Indonesian Journal of Aquaculture Medium
Publisher : Program Studi Budidaya Perairan Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/mediaakuakultur.v5i2.7332

This study aims to evaluate the financial performance of the koi fish farming industry (Cyprinus rubrofuscus) in Indonesia through a selective breeding program. Data collected between 2021 and 2024, covering business development, operational costs, investment costs, and capital estimates, was obtained from 24 koi fish farming businesses in the Java region. Businesses using genetic screening aim to reduce financial disparities, which is different from traditional aquaculture methods. The analysis's results indicate that the implementation of the selective breeding program in a scientific manner yielded a higher profit margin of 34.2% than the traditional method, which only reached 18.7%. Although the initial investment is more substantial (about Rp28,5 million per hectare), the time frame required to pay for the investment is more efficient, i.e., between 18 and 24 months. The price of koi fish is higher than the market price, possibly reaching 2.8% of the price of koi fish, with the selling price per tail ranging from Rp450,000 to Rp1,200,000. In addition, there was a 15% increase in feed efficiency and a 3% decrease in mortality, which means a 7.8% reduction. Thus, this selective breeding program can improve koi farming production and sales performance by applying genetic technology, which can help overcome the financial crisis in the aquaculture sector in developing countries.

Analisis Kualitas Perairan Berdasarkan Tingkat Kesuburan dan Status Pencemaran Air Pesisir Bomo, Banyuwangi, Indonesia: Analysis of Water Quality Based on Trophic Status and Pollution Status in Bomo Coastal Waters, Banyuwangi, Indonesia Dewi, Nina Nurmalia; Nindarwi, Daruti Dinda; Sari, Putri Desi Wulan; Fauzan, Agung Luthfi
JFMR (Journal of Fisheries and Marine Research) Vol. 9 No. 3 (2025): JFMR on November
Publisher : Faculty of Fisheries and Marine Science, Brawijaya University, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jfmr.2025.009.03.4

Perairan pesisir Bomo Banyuwangi merupakan wilayah pesisir yang potensial yang menjadi habitat bagi berbagai organisme akuatik. Adanya pengaruh antropogenik dapat berpotensi mempengaruhi kondisi perairan. Penelitian ini bertujuan untuk mengetahui kondisi lingkungan perairan berdasarkan penentuan tingkat kesuburan dan status pencemaran air pesisir Bomo. Penelitian dilaksanakan di wilayah pesisir Bomo dengan pengambilan sampel di tiga stasiun yang diambil berdasarkan jarak perairan dari daratan. Setiap stasiun diambil sampel sebanyak 3 kali ulangan yang diambil selama 2 bulan pengamatan. Pengambilan sampel meliputi parameter kualitas air yang terdiri dari parameter fisika, kimia, dan biologi yang dianalisis secara insitu maupun exsitu. Data kualitas air dianalisis tingkat kesuburannya menggunakan pendekatan Trophic State Index (TSI) dan Trophic Level Index (TLI). Status pencemaran air dianalisis berdasarkan indeks storet dan indeks pencemaran (pollution index). Berdasarkan hasil penelitian, didapatkan bahwa tingkat kesuburan perairan berada pada kategori eutrofik dengan nilai TSI 74,31-76,58 dan super-eutrofik dengan nilai TLI 6,39-6,84, sedangkan status pencemaran air dipesisir Bomo tergolong memiliki pencemaran yang tinggi dengan nilai indeks storet -37 dan -41 pada bulan Juli dan September dan berdasarkan indeks pencemaran (IP) memiliki nilai 9,10 dan 5,77 untuk masing-masing bulan Juli dan September. Hal ini menandakan bahwa adanya masukan limbah ke perairan yang mempengaruhi kondisi perairan pesisir Bomo. Bomo Coastal Water Banyuwangi are a potential coastal area that is a habitat for various aquatic organisms. The presence of anthropogenic activity can potentially affect the condition of the waters. This study aims to determine the condition of the aquatic environment based on determining the level of trophic status and pollution status of the Bomo coastal waters. The study was conducted in the coastal area of Bomo by taking samples at three stations taken based on the distance of the waters from the mainland. Each station was sampled 3 times during 2 months of observation. Sampling included water quality parameters consisting of physical, chemical, and biological parameters which were analyzed in situ and ex situ. Water quality data were analyzed for their trophic status using the Trophic State Index (TSI) and Trophic Level Index (TLI) approaches. Pollution status was analyzed based on the storet index and pollution index. Based on the results of the study, it was found that the level of water trophic status was in the eutrophic category with a TSI value of 74.31-76.58 and super-eutrophic with a TLI value of 6.39-6.84, while the status of water pollution on the Bomo coastal waters was classified as having high pollution with a Storet Index value of -37 and -41 in July and September and based on the Pollution Index had a value of 9.10 and 5.77 for July and September respectively. This indicates that there is waste input into the waters that affects the condition of the Bomo coastal waters.

Title

Found 6 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 6 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 6 Documents
Search