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Phenotype of the First Gynogenesis Generation of Koi Alimuddin, ,; Sumantadinata, K.; Hadiroseyani, Yani; Irawan, D.
Jurnal Akuakultur Indonesia Vol. 1 No. 2 (2002): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACTThis experiment was conducted to study phenotype of F1 koi that obtained from gynogenesis at the Laboratory of Fish Genetic and Breeding, Department of Aquaculture, Faculty of Fisheries and Marine Sciences, Bogor Agricultural University (IPB). Females koi used for this experiment were kohaku (white-red), hi-utsuri (red-black), and shiro-bekko (white-black); whereas males used kohaku, hi-utsuri, and shiro-bekko. Analysis on body coloration of fish was carried out at three months old. Results showed that gynogenesis from kohaku produced three types of koi, those were white koi, red koi and kohaku, and hi-utsuri produced red koi, black koi and hi-utsuri. Meanwhile, shiro-bekko by gynogenetic technique produce seven types of koi; those were white, red, black, kohaku, shiro-bekko, hi-utsuri and sanke (white-red-black koi). Survival rate of gynogenetic koi was lower then normal might be due to inbreeding stress.Key words : Gynogenesis, phenotype, koi fish (Cyprinus carpio). ABSTRAKStudi tentang fenotip keturunan pertama ikan koi hasil ginogenesis telah dilakukan di Laboratorium Pengembangbiakan dan Genetika Ikan, Jurusan Budidaya Perairan, Fakultas Perikanan dan Ilmu Kelautan IPB. Ikan koi betina yang dipakai adalah kohaku (putih-merah), hi-utsuri (merah-hitam) dan shiro-bekko (putih-hitam), sedangkan jantannya adalah kohaku, hi-utsuri, dan shiro-bekko. Analisis warna pada ikan dilakukan setelah ikan berumur tiga bulan. Hasil penelitian menunjukkan bahwa ginogenesis pada ikan kohaku menghasilkan tiga jenis ikan koi, yaitu koi putih, koi merah dan kohaku; pada ikan hi-utsuri dihasilkan ikan koi merah, koi hitam dan hi-utsuri. Sementara itu, teknik ginogenesis untuk ikan koi putih-hitam dihasilkan tujuh macam jenis ikan koi, yaitu koi putih, koi merah, koi hitam, kohaku, hi-utsuri, siro-bekko dan sanke (putih-merah-hitam). Tingkat kelangsungan hidup ikan ginogenetik lebih rendah daripada kontrol normalnya.Kata kunci : Ginogenesis, fenotip, ikan koi (Cyprinus carpio)

Sex Reversal on Congo Tetra Fish (Micraleptus intterruptus ) Larvae Arfah, Harton; Alimuddin, ,; Sumantadinata, K.; Ekasari, Julie
Jurnal Akuakultur Indonesia Vol. 1 No. 2 (2002): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACTExperiment was performed to assess the effect of 17a-methyltestosterone (MT) treatment on Congo tetra fish larvae. To evaluate the optimal pattern of MT treatment, three different treatments were administrated. Three months old larvae were submerged in three different doses of MT; 1, 2 and 4 mg/l. These studies showed that the highest percentage of male fish was obtained by 4 mg/l MT treatment, 87,17%. The 2 mg/l and 1 mg/l MT treatments obtained 77,53% and 69,86% male respectively, two times higher than control, 38,96%. On the other hand, the 4 mg/l MT treatment also resulted the highest percentage of hermaphrodite fishes, 17,58%. The highest survival rate was shown by 1 mg/l MT treatment, 62,77% and the lowest was shown by the 4 mg/l MT treatment, 47,20%. The highest rate of fish length and weight was shown by the 4 mg/l MT treatment, 4,4 cm and 1,65 gram respectively. These findings suggest that MT treatment offers an advantage in growth of tetra Congo larvae. Key word : Sex reversal, methyltestosterone, Congo tetra fish, Micraleptus intterruptus. ABSTRAKPenelitian ini bertujuan untuk mengetahui pengaruh perendaman larva di dalam larutan hormon 17a-metiltestosteron pada dosis 1, 2 dan 4 mg/l larutan. Persentase tertinggi ikan jantan dihasilkan oleh perlakuan 4 mg/l, yaitu 87,17%. Perlakuan 2 mg/l dan 1 mg/l masing-masing menghasilkan 77,53% dan 69,86% sedangkan kontrol menghasilkan 38,96% jantan. Efek lain dari perlakuan MT ini adalah hermafroditisme. Perlakuan 4 mg/l menghasilkan persentase hermafrodit tertinggi yaitu 17,58%, sedangkan pada kontrol kelangsungan hidup tertinggi diperoleh pada perlakuan 1 mg/l (62,77%) dan terendah pada perlakuan 4 mg/l (47,20%). Hasil tersebut menunjukkan adanya pengaruh dosis hormon terhadap kelangsungan hidup ikan. Pengukuran bobot dan panjang ikan pada setiap perlakuan menunjukkan nilai tertinggi dihasilkan oleh perlakuan 4 mg/l yaitu 1,65 gram dan 4,40 cm. Hal ini diduga bahwa hormon MT merangsang pula pertumbuhan ikan.Kata kunci : Pergantian kelamin, metiltestosteron, ikan tetra Kongo, Micraleptus intterruptus.

Growth and survival of giant gourami juvenile immersed in different doses of recombinant growth hormone Alimuddin, ,; Etoh, Sumie; Pramana Putra, Handika Gilang; Carman, Odang
Jurnal Akuakultur Indonesia Vol. 10 No. 2 (2011): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRAKPenelitian ini dilakukan untuk meningkatkan pertumbuhan benih ikan gurami (Osphronemus goramy) melalui imersi dengan hormon pertumbuhan rekombinan ikan gurami (r-OgGH). Imersi dengan larutan mengandung badan inklusi dilakukan dengan dosis r-OgGH berbeda, yakni 0 mg/L (kontrol), 10 mg/L, 20 mg/L, dan 30 mg/L, sekali seminggu selama tiga minggu. Ikan dipelihara selama lima minggu dalam akuarium dengan kepadatan 5 ekor/L, dan ikan diberi pakan naupli Artemia dan cacing sutera dua kali sehari secara ad libitum. Hasil penelitian menunjukkan bahwa rerata bobot tubuh ikan perlakuan 30 mg/L (0,34 g) adalah sekitar 75% lebih tinggi (p<0,05) daripada perlakuan 10 mg/L (0,24 g) dan kontrol (0,19 g). Kelangsungan hidup tertinggi diperoleh pada perlakuan 30 mg/L (100%), diikuti oleh perlakuan 20 mg/L (96%), perlakuan 10 mg/L (94%), dan kontrol (94%). Dengan demikian, imersi menggunakan hormon pertumbuhan rekombinan dapat digunakan untuk meningkatkan pertumbuhan dan kelangsungan hidup benih ikan gurami.Kata kunci: protein rekombinan, hormon pertumbuhan, imersi, Osphronemus goramy ABSTRACTThis study was aimed at enhancing the growth of giant gourami Osphronemus goramy fry by immersion with recombinant giant gourami growth hormone (r-OgGH). Immersion with solution containing inclusion bodies with different doses of r-OgGH, 0 mg/L (control), 10 mg/L, 20 mg/L, and 30 mg/L was performed on weekly basis for the first three weeks of experimental period. Fish were reared for five weeks in glass aquaria at a stocking density of 7 fish/L, and fed with Artemia nauplii and blood worm twice a day ad libitum. The results showed that the average body weight of 30 mg/L r-OgGH treated fish (0.34 g) was 75% higher (p<0.05) than those of 10 mg/L (0.24 g) and control (0.19 g). The highest survival was obtained at 30 mg/L r-OgGH immersed fish (100%), followed by 20 mg/L (96%) and 10 mg/L treated fish (94%), and control (94%). The results indicate that immersion with recombinant growth hormone could be applied to enhance the growth and survival of giant gourami juvenile.Keywords: recombinant protein, growth hormone, immersion, Osphronemus goramy

Growth of white shrimp post-larvae immersed in recombinant fish growth hormone Laksana, Dita Puji; Subaidah, Siti; Junior, Muhammad Zairin; Alimuddin, ,; Carman, Odang
Jurnal Akuakultur Indonesia Vol. 12 No. 2 (2013): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

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ABSTRACT This research was conducted to determine the optimum immersion time of recombinant giant grouper growth hormone (Ephinephelus lanceolatus; rElGH) at a dose of 15 mg/L that generated highest growth of white shrimp post-larvae (PL). PL was bath-immersed for one, two, and three hours. Two types of control was provided, namely it was without any treatment (control), and immersion in water containing 0.01% bovine serum albumin (BSA) and total protein of Escherichia coli without rElGH (pCold control). All treatments and controls were consisted of three replications. A total of 1,500 PL-2 shrimp were bath-immersed in a plastic packing containing 1 L of sea water, 15 mg/L rElGH, and 0.01% BSA. PL was further reared for 21 days in the 60 L glass aquarium, and fed nauplii Artemia two times and flake commercial diet five times daily, at satiation. The results showed that the highest of biomass (36.29±1.46 g), specific growth rate (29.81±0.87%/day), and body length (20.08±0.42 mm) were obtained in three hours immersion treatment (P<0.05). Biomass of PL in three hours immersion treatment was approximately 66.0% higher compared to the control (21.87±2.53 g). Survival of shrimp in all treatment and control were similar (P>0.05). Thus, growth of white shrimp PL could be improved by bath immersion for three hours in rElGH solution of 15 mg/L water. Keywords: recombinant growth hormone, different immersion time, Pacific white shrimp, biomass ABSTRAK Penelitian ini dilakukan untuk menentukan lama waktu perendaman hormon pertumbuhan rekombinan ikan kerapu kertang (Ephinephelus lanceolatus; rElGH) dosis 15 mg/L yang menghasilkan pertumbuhan tertinggi pada pascalarva (PL) udang vaname. Perendaman PL dilakukan satu, dua, dan tiga jam. Dua jenis kontrol dibuat yakni udang vaname PL-2 tidak diberi perlakuan (kontrol), dan direndam dalam air mengandung serum albumin sapi (BSA) 0,01% dan protein Escherichia coli tanpa rElGH (kontrol pCold). Setiap perlakuan dan kontrol diberi tiga ulangan. Sebanyak 1.500 ekor PL-2 direndam dalam kantong plastik kemasan berisi 1 L air laut mengandung rElGH 15 mg/L, dan BSA 0,01%. Selanjutnya, udang dipelihara selama 21 hari di dalam akuarium volume 60 L, dan diberi pakan naupli Artemia sebanyak dua kali dan pakan komersial berbentuk flake sebanyak lima kali sehari hingga kenyang. Hasil penelitian menunjukkan bahwa biomassa (36,29±1,46 g), pertumbuhan bobot spesifik (29,81±0,87%/hari), dan panjang tubuh (20,08±0,42 mm) tertinggi diperoleh pada perlakuan perendaman selama tiga jam (P<0,05). Biomassa udang perlakuan perendaman selama tiga jam lebih tinggi 66% dibandingkan dengan kontrol (21,87±2,53 g). Kelangsungan hidup udang yang direndam dengan rElGH, kontrol dan kontrol pCold tidak berbeda nyata (P<0,05). Dengan demikian, pertumbuhan PL udang vaname dapat ditingkatkan melalui perendaman selama tiga jam dalam larutan rElGH 15 mg/L air. Kata kunci: hormon pertumbuhan rekombinan, lama perendaman, pascalarva udang vaname, biomassa

Testicular cell transplantation of neon tetra Paracheirodon innesi into common carp fry Alimuddin, ,; Carman, Odang; Wulandari, Sri Setyo
Jurnal Akuakultur Indonesia Vol. 12 No. 2 (2013): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT Neon tetra Paracheirodon innesi is an ornamental fish that have high export value. However, production is still relatively low due to low fecundity (approximately 180 eggs/female). Technology of testicular cell transplantation of neon tetra as donor to common carp as recipient fish which have high fecundity provides a promising way to overcome problem of neon tetra production. This research was performed to determine the optimum age of common carp fry that is able to receive donor cells and allow high success of transplantation. In this research, the testes of neon tetra fish were dissociated by 0.5% trypsin solution. The testicular cells were labeled with PKH-26 fluorescent dye, and then transplanted into the peritoneal cavity of seven, ten, and 14 days post hatching common carp fry. The results showed that the survival of seven day-old transplanted fry (31.25%) was lower than that of ten day-old (37.75%) and 14 day-old transplanted fry (56.25%). Percentage of fish colonized testicular cells donor at 21 days post-transplantation on seven days old and ten days old fry were similar (80%), while on 14 day-old fry was 60%. Based on the cumulative transplantation success rate (survival and colonization rates), transplantation on 14 days old fry (33.75%) showed higher result compared to transplantation on seven days old fry (25.00%) and ten day-old fry (30.00%). It can be concluded that transplantation of neon tetra testicular cells to common carp fry have been successfully carried out, and the optimum age of common carp fry to transplantation was 14 days after hatching. Keywords: transplantation, colonization, testicular cells, common carp, neon tetra ABSTRAK Ikan neon tetra Paracheirodon innesi merupakan ikan hias yang memiliki nilai ekspor yang tinggi. Namun demikian, tingkat produksinya masih relatif rendah karena fekunditas ikan neon tetra yang sedikit (sekitar 180 telur/induk). Teknologi transplantasi sel testikular ikan neon tetra (ikan donor) ke ikan mas yang memiliki fekunditas telur yang banyak dan diharapkan mampu mengatasi ketersediaan benih ikan neon tetra. Penelitian ini bertujuan untuk menentukan umur optimum benih ikan mas (calon ikan semang) yang mampu menerima sel donor dengan baik dan memiliki keberhasilan kolonisasi yang tinggi. Testis ikan neon tetra didisosiasi menggunakan larutan tripsin 0,5%. Sel testikular diwarnai dengan PKH-26, kemudian ditransplantasikan ke rongga peritoneal benih ikan mas umur tujuh, sepuluh, dan 14 hari setelah menetas. Hasil penelitian menunjukkan bahwa tingkat kelangsungan hidup ikan mas perlakuan transplantasi umur tujuh hari (31,25%) lebih rendah dibandingkan dengan perlakuan transplantasi umur sepuluh hari (37,50%) dan 14 hari (56,25%). Persentase ikan terkolonisasi sel donor pada hari ke-21 pascatransplantasi pada benih umur tujuh dan sepuluh hari adalah sama (80%), sedangkan transplantasi benih umur 14 hari sebesar 60%. Berdasarkan keberhasilan transplantasi secara kumulatif (tingkat kelangsungan hidup dan kolonisasi), transplantasi pada benih umur 14 hari (33,75%) menunjukkan hasil lebih tinggi dibandingkan dengan perlakuan transplantasi pada benih umur tujuh hari (25,00%) dan benih umur sepuluh hari (30,00%). Transplantasi sel testikular ikan neon tetra pada benih ikan mas telah berhasil dilakukan, dan umur optimum benih ikan mas adalah 14 hari setelah menetas. Kata kunci: transplantasi, kolonisasi, sel testikular, ikan mas, ikan neon tetra

Frequency and persistency of DNA vaccine encoding GP25 by oral on common carp Nuryati, Sri; Yuliyanti, ,; Alimuddin, ,
Jurnal Akuakultur Indonesia Vol. 12 No. 2 (2013): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT Koi herpesvirus (KHV) is a major viral pathogen that infects common carp and koi. KHV disease outbreak is happened in almost all centre of common carp culture in Indonesia and caused mass mortality. Deoxyribonucleic acid (DNA) vaccination method is one of ways to cope with KHV infection. Vaccines were commonly given by injection. The aim of this research was to get frequency and persistency of DNA vaccine encoding GP25 given by oral delivery method in common carp. This research would like to determine dose, frequency of vaccination, persistency of DNA vaccine and culture medium for the bacterial host. DNA vaccine persistency test was done by using polymerase chain reaction (PCR) method with the specific primer for GP25 gene. The results showed that level of DNA vaccine that could be detected in feed was 7.56 ng (equal to 1.598×1010 copies). Efficient culture medium for Escherichia coli DH5α carrying DNA vaccine was LB triptone. Feeding fish with diet supplemented with 1 mL E. coli DH5α containing DNA vaccine for each fish and two times a week allowed persistence of DNA vaccine in kindney and spleen. Keywords: common carp, KHV, DNA vaccine, GP25, persistance ABSTRAK Koi herpesvirus (KHV) adalah virus patogen utama yang menginfeksi ikan mas dan ikan koi. Wabah penyakit KHV terjadi di hampir semua sentra budidaya ikan mas di Indonesia dan menyebabkan kematian massal ikan. Metode vaksinasi DNA merupakan salah satu cara yang dapat dilakukan untuk menanggulangi serangan KHV. Pemberian vaksin umumnya dilakukan dengan cara injeksi. Tujuan penelitian ini adalah untuk menguji frekuensi dan persistensi vaksin DNA GP25 antivirus KHV yang diberikan melalui oral pada ikan mas. Pada penelitian ini dilakukan uji dosis, frekuensi pemberian vaksin, persistensi vaksin DNA, dan media kultur bakteri inang. Persistensi vaksin DNA dianalisis menggunakan metode PCR dengan primer spesifik gen GP25. Hasil penelitian menunjukkan bahwa dosis vaksin DNA yang dapat terdeteksi dalam pakan adalah 7,56 ng (setara dengan 1,598×1010 copy). Media kultur yang efisien bagi bakteri Escherichia coli DH5α pembawa vaksin DNA adalah LB tripton. Pemberian pakan bervaksin berupa bakteri konsentrasi 1 mL/ekor ikan dengan frekuensi dua kali seminggu menghasilkan persistensi DNA GP25 di ginjal dan limpa. Kata kunci: ikan mas, KHV, vaksin DNA, GP25, persistensi

Electroporation and GFP-labelled transplantation of testicular cells in Nile tilapia Barades, Epro; Alimuddin, ,; Sudrajat, Agus Oman
Jurnal Akuakultur Indonesia Vol. 12 No. 2 (2013): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT Transplantation technology can be applied to generate fish surrogate broodstock. A donor germinal cells that was used in transplantation are labeled to distinguish it with endogenous cells. Donor cells are generally derived from transgenic fish carrying a marker or cells labeled by PKH-26. This study was performed to obtain an alternative method of cell labelling using electroporation. Testicular cells were taken from 4-months old Nile tilapia as a model. Electroporation was performed with testicular cell density of 104 cells/µL, pJfKer-GFP concentration of 50 ng/µL, and a pulse length of 20 ms at 0, 100, 200, and 300 volts. At amount of 5x103 cells/0.5µL electroporated testicular cells were then injected into the intraperitoneal cavity of 3-day-old Nile tilapia larva. The results showed that survival of the electroporated cells of 100 and 200 volt-treatments was similar (P>0.05), and higher than 300 volt (P<0.05). Number of fluorescent cells was not significantly different among treatments. The highest cell colonization in transplanted fish was obtained in 200-volt treatment (66.67%). As conclusion, 200-volt electroporation with was a suitable tool to label testicular cells for transplantation. Keyword: electroporation, GFP, label, Nile tilapia, transplantation ABSTRAK Teknologi transplantasi merupakan suatu teknologi yang dapat menghasilkan induk pengganti. Sel donor berupa sel germinal yang akan digunakan dalam transplantasi diberi label agar dapat dibedakan dengan sel resipien. Umumnya sel donor diperoleh dari ikan transgenik yang membawa marka atau diwarnai dengan PKH-26. Penelitian ini bertujuan untuk mencari metode alternatif dalam pemberian label pada sel dengan elektroporasi. Sel testikular diperoleh dari ikan nila berumur empat bulan sebagai model. Elektroporasi dilakukan dengan kepadatan sel 104 sel/µL, konsentrasi pJfKer-GFP 50 ng/µL, dan panjang kejut 20 ms pada 0, 100, 200, dan 300 volt. Sebanyak 5x103 sel dalam 0,5 µL larutan hasil elektroporasi disuntikkan ke dalam rongga intraperitoneal larva berumur tiga hari setelah menetas. Hasil penelitian menunjukkan bahwa kelangsungan hidup sel pada kejut 100 dan 200 volt tidak signifikan (P<0,05), akan tetapi lebih tinggi bila dibandingkan dengan perlakuan 300 volt (P<0,05). Jumlah sel yang berpendar tidak berbeda antara perlakuan. Persentase kolonisasi sel pada ikan hasil transplan tertinggi pada perlakuan 200 volt (66,67%). Kesimpulan yang diperoleh dari hasil penelitian adalah perlakuan elektroporasi dengan kejut listrik 200 volt dapat digunakan untuk memberi label pada sel testikular yang akan ditransplantasikan. Kata kunci : elektroporasi, GFP, label, ikan nila, transplantasi

Survival of common carp carrying Cyca-DAB1*05 post-challenged with Aeromonas hydrophila Arsal, Laode Muhammad; Yuhana, Munti; Nuryati, Sri; Alimuddin, ,
Jurnal Akuakultur Indonesia Vol. 13 No. 2 (2014): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT Blood parameters are considered as important indicators to diagnose fish health status. This study was performed to observe blood profiles including total erythrocytes, hemoglobin concentration, hematocrite, total leukocytes and differential leukocytes, and survival of common carp Cyprinus carpio infected by Aeromonas hydrophila. Fish were divided into two groups: the 3rd generation of common carp carrying fish carrying Cyca-DAB1*05 of major histocompatibility complex II molecular marker, and fish without the marker as control treatment. histocompatibility complex II molecular marker, and fish without the marker as control treatment. Common carp 3rd generation was produced by crossing among second generation of fish carrying the Cyca-DAB1*05 marker. Each fish was injected intramuscularly by 0.1 mL of 108 cfu/mL A. hydrophila. Challenge test was conducted for 14 days and blood was collected at day-0, three, seven, and 14. The results of this study showed that erythrocytes, hemoglobin and hematocrite concentrations of common carp carrying the molecular marker at post challenge with A. hydrophila were higher (P<0.05) compared to control fish. The blood profiles were highly correlated to survival of fish. Survival of fish that carrying the molekuler marker was about two point six fold higher than those of control fish. Keywords: Cyprinus carpio, Aeromonas hydrophila, Cyca-DAB1*05, molecular marker ABSTRAK Gambaran darah merupakan indikator penting untuk mendiagnosa penyakit ikan. Penelitian ini dilakukan untuk meguji gambaran darah ikan mas Cyprinus carpio setelah diinfeksi dengan bakteri Aeromonas hydrophila yang meliputi total sel darah merah, konsentrasi hemoglobin, hematokrit, total sel darah putih, dan diferensial leukosit, serta sintasannya. Ikan mas yang digunakan terdiri atas ikan mas generasi ketiga yang membawa marka molekuler Cyca-DAB1*05 dari kelompok major histocompatibility complex II dan ikan mas tanpa marka sebagai kontrol. Ikan mas generasi ketiga merupakan keturunan persilangan antarikan mas generasi kedua yang mempunyai marka Cyca-DAB1*05. Masing-masing ikan diinfeksikan A. hydrophila secara intramuskuler pada dosis 0,1 mL, kepadatan 108 cfu/mL. Uji tantang dilakukan selama 14 hari, dan sampel darah ikan diambil pada hari ke-0, tiga, tujuh dan 14. Hasil uji tantang menunjukkan bahwa total sel darah merah, konsentrasi hemoglobin dan hematokrit pada ikan mas yang membawa marka molekuler lebih tinggi dibandingkan ikan mas tanpa marka (P<0,05). Hasil uji gambaran darah berkorelasi tinggi dengan tingkat kelangsungan hidup ikan mas. Tingkat kelangsungan hidup ikan mas yang membawa marka molekuler Cyca-DAB1*05 lebih tinggi hingga dua koma enam kali daripada ikan mas kontrol. Kata kunci: Cyprinus carpio, Aeromonas hydrophila, Cyca-DAB1*05, marka molekuler

Induced maturation of eel Anguilla bicolor using different hormone combination Sudrajat, Agus Oman; Sugati, Antharest; Alimuddin, ,
Jurnal Akuakultur Indonesia Vol. 13 No. 2 (2014): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT Artificial reproduction of eel Anguilla bicolor is not yet well-established because of insufficient broodstock number. In this research, induction of Indonesian eel gonad maturation was performed by hormonal with a combination of pregnant mare serum gonadotropin (PMSG), human chorionic gonadotropin (HCG) antidopamin and recombinant growth hormone (rGH). This research consisted of five treatments namely: control (NaCl 0,9%), PMSG 20 IU/ kg, PMSG 20 IU/kg + antidopamin 10 ppm/kg, PMSG 20 IU/kg + antidopamin 10 ppm/kg + rGH 10 μg/kg dan PMSG 20 IU/kg + HCG 10 IU/kg. Each treatment contained 10 fishes. Hormonal induction was conducted by intramuscular injections, as much as five times at intervals of seven days. Furthermore observations on gonadal development were performed after injection for 21 days. The results showed that the treatment generated pregnancy level of 100%, while control was 0%. The best treatment was PMSG 20 IU/kg + antidopamin 10 ppm/kg+ rGH 10 μg/kg, seen from a more mature phase of the gametes, spermatocytes in male and oocytes with perinukleolar phase in female fish. Eel at the body weight of 120.4 to 207.8 g and at the body length of 40.9 to 43.1 cm was male, at the body weight of 274.8 g and at the body length of 47 cm was in intersexual phase, and at the body weight of 323.4 g and at the body length of 53 cm was female. Keywords: Anguilla bicolor, antidopamin, hormones, PMSG, rGH, HCG ABSTRAK Pemijahan ikan sidat secara buatan belum dapat dilakukan karena keterbatasan induk matang gonad. Penelitian ini dilakukan untuk mengetahui pengaruh pemberian hormon terhadap percepatan proses perkembangan gonad ikan sidat (Anguilla bicolor). Hormon yang digunakan adalah kombinasi dari pregnant mare serum gonadotropin (PMSG), human chorionic gonadotropin (HCG), antidopamin dan recombinant growth hormone (rGH). Induksi hormonal untuk mempercepat perkembangan gonad ikan sidat dilakukan melalui lima perlakuan yaitu yaitu kontrol (NaCl 0,9%), PMSG 20 IU/kg, PMSG 20 IU/kg+antidopamin 100 ppm/kg, PMSG 20 IU/kg+antidopamin 100 ppm/ kg+rGH 10 μg/kg dan PMSG 20 IU/kg+HCG 10 IU/kg. Setiap perlakuan dilakukan pada sepuluh ekor ikan sidat. Aplikasi induksi hormonal dilakukan melalui penyuntikan secara intramuskular sebanyak lima kali dengan interval tujuh hari sekali, selanjutnya dilakukan pengamatan terhadap perkembangan gonad selama 21 hari dengan interval tujuh hari sekali setelah penyuntikan. Hasil penelitian menunjukkan bahwa perlakuan hormonal menyebabkan tingkat kebuntingan sebanyak 100% pada ikan perlakuan, sedangkan kontrol sebanyak 0%. Kombinasi terbaik adalah PMSG+antidopamin+rGH, terlihat dari fase gamet yang lebih matang yaitu mencapai fase spermatosit pada ikan jantan dan oosit dengan fase perinukleolar pada ikan betina. Berdasarkan hasil penelitian, ikan sidat dengan bobot 120,4−207,8 g dan panjang 40,9−43,1 cm masih berjenis kelamin jantan. Ikan dengan bobot 274,8 g dan panjang 47 cm masih berada pada fase peralihan kelamin, sedangkan pada bobot 323,4 dan panjang 53 cm sudah berjenis kelamin betina. Kata kunci: Anguilla bicolor, antidopamin, hormon, PMSG, rGH, HCG

Potential transmission test of GP25 vaccine in normal flora bacteria of common carp culture media Nuryati, Sri; Alimuddin, ,; Juliadiningtyas, Ayu Dhita
Jurnal Akuakultur Indonesia Vol. 14 No. 1 (2015): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT Koi herpesvirus (KHV) is a virus that infects common carp and koi Cyprinus carpio. KHV attacks most stadia of common carp and koi and causes mortality up to 80–95% of the population. One way to prevent the spread of KHV is by applying DNA vaccine. This research was conducted to test potential transmission of DNA vaccine encoding glycoprotein 25 (vaccine GP25) toward normal flora bacteria of media cultivation of common carp. Bacteria was isolated from pond water of common carp and tested for their sensitivity to ampicillin. Research was performed by adding vaccine GP25 to ampicillin-sensitive bacteria at a dose of 12.5 µg/100 µL and incubated at 28 °C for 30, 60, 180, and 300 minutes then plated on media containing ampicillin. The grown bacteria cells were tested for the existence of plasmid bearing gen GP25 through bacteria colony cracking. The results of this research showed that there was no bacteria contained plasmid bearing gen GP25. Keywords: DNA vaccines, common carp, vaccine safety ABSTRAK Koi herpesvirus (KHV) merupakan virus yang menginfeksi ikan mas dan koi Cyprinus carpio. KHV menyerang hampir semua stadia ikan mas dan koi dan menyebabkan kematian hingga 80–95% dari populasi. Salah satu cara penanggulangan penyebaran KHV adalah dengan penggunaan vaksin DNA. Penelitian ini dilakukan untuk menguji potensi transmisi vaksin DNA glikoprotein 25 (vaksin GP25) pada bakteri flora normal pada media budidaya ikan mas. Bakteri diisolasi dari kolam budidaya ikan mas dan diuji sensitivitasnya terhadap antibiotik ampisilin. Penelitian dilakukan dengan menambahkan vaksin GP25 ke larutan bakteri sensitif ampisilin dengan dosis 12,5 µg/100 µL dan diinkubasi pada suhu 28 °C selama 30, 60, 180, dan 300 menit kemudian disebar pada media mengandung antibiotik ampisilin. Sel bakteri yang tumbuh diuji untuk mendeteksi keberadaan plasmid pembawa gen GP25 dengan menggunakan metode seleksi koloni bakteri (colony cracking). Hasil penelitian ini menunjukkan bahwa tidak ada bakteri yang mengandung plasmid pembawa gen GP25. Kata kunci: keamanan vaksin, ikan mas, vaksin DNA

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