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Journal : Kultivasi

The effect of tryptone and tomato juice addition on the growth performance of strawberry (Fragaria ananassa) explant under in vitro condition Ulinnuha, Zulfa; Tini, Etik Wukir; Farid, Noor; Shalatin, Dhianing
Kultivasi Vol 23, No 1 (2024): Jurnal Kultivasi
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/kultivasi.v23i1.50082

Abstract

Strawberries are a fruit commodity that has high economic value. The obstacle in strawberry production is the lack of high-quality seed availability with disease-free properties. Tissue culture offers a promising solution to increase both the quantity and quality of strawberry seeds. This study aims to assess the effect of different concentrations of tomato juice and tryptone on the growth of strawberry explants under in vitro growing condition. The research was conducted at the Plant Breeding Laboratory, Faculty of Agriculture, Universitas Jenderal Soedirman, from December 2022 to May 2023. The study employed a Completely Randomized Design (CRD) with two factors. The first factor was tomato juice (Z), consisting of Z0 = 0 mL/L, Z1 = 50 mL/L, Z2 = 100 mL/L. The second factor was tryptone (T), consisting of T0 = 0 g/L, T1 = 1 g/L, T2 = 2 g/L, T3 = 3 g/L. The addition of 50 mL/L tomato juice solely (T0Z1) resulted in the highest plant height, number of leaves, number of branches, and leaf width. Meanwhile, the addition of2 g/L tryptone solely showed the highest germination rate.
Agronomic biofortification of Calcium in Pak choy (Brassica rapa subsp. chinensis) via hydroponic nutrient film technique Zahro, Regina Septiani; Faozi, Khavid; Ulinnuha, Zulfa; Sarjito, Agus
Kultivasi Vol 23, No 3 (2024): Jurnal Kultivasi
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/kultivasi.v23i3.56760

Abstract

Pak choy is rich in calcium, magnesium, phosphorus, iron, and vitamin K, all essential for health. Pak choy requires a proper balance of nutrients for optimal growth. In a hydroponic system, any imbalance in nutrient levels can negatively impact plant growth and reduce the nutritional value stored in the tissues.  Selecting suitable varieties and providing appropriate calcium treatment can help increase calcium content and enhance productivity. This study aims to determine the effect of calcium concentration on growth, yield, and Ca content and the most responsive varieties to increased calcium concentration. The research design used was a Split Plot Design with two factors, namely calcium nitrate concentration (k) as the main plot consisting of k0 = without addition of Ca(NO3)2, k1 = addition of 300 mg/L Ca(NO3)2, k2 = addition of 600 mg/L Ca(NO3)2, k3 = addition of 900 mg/L Ca(NO3)2, and k4 = addition of 1200 mg/L Ca(NO3)2. Varieties (v) as subplots consist of v1 = Masbro, v2 = Nauli F1, and v3 = Flamingo. The results showed that the Nauli F1 variety had the best effect on the number of leaves, growth rate index, and fresh weight of shoots. Adding 900 mg/L of calcium nitrate had the best impact on plant growth, while adding 1200 mg/L of calcium nitrate caused plant poisoning. The Masbro variety was responsive to Ca biofortification.
Phenotypic evaluation of F10 soybean generations from Grobogan x Slamet cross for large seed size selection Hidayat, Ponendi; Nurtiati, Nurtiati; Ulinnuha, Zulfa; Adellansyah, Adellansyah
Kultivasi Vol 24, No 2 (2025): Jurnal Kultivasi
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/kultivasi.v24i2.62772

Abstract

Soybeans are an important crop with high nutritional value and diverse uses. Current consumer and industry preference is for soybean varieties with large seeds and high productivity. To meet these demands, one effective approach is through hybridization. This study aims to evaluate the phenotype of F10 soybean line from a cross between Grobogan and Slamet for large-seeded and high-yield. The study was conducted in the screenhouse and Plant Breeding and Biotechnology Laboratory, Faculty of Agriculture, Jenderal Soedirman University. The experiment used a Randomized Complete Block Design (RCBD) with three replications, testing genotypes from Slamet x Grobogan crosses and three check varieties: Slamet, Grobogan, and Wilis. Observational data were analyzed using analysis of variance (ANOVA) with a 5% error rate and continued with LSD to select lines with high-performing genotype. The results of the ANOVA showed that the tested lines affected growth parameters and plant yield components. LSD analysis showed that the highest leaf length and number of leaves were in GS 7. In seed weight per plant, all lines were below Grobogan, followed by GS 7 and GS 47. In 100 seed weight, all lines were below Grobogan, but there were lines with large seed categories (> 14 g/100 seeds), namely GS7, GS 12, GS 36, and GS 39. Correlation analysis showed that the number of pods and 100 seed weight were positively correlated with seed weight per plant. Therefore, these traits can be selection indicators to identify high-yielding soybean genotype.