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All Journal Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Bioeksperimen: Jurnal Penelitian Biologi Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan) BIOPROSPEK Jurnal Riset Akuakultur Aceh Journal of Animal Science
Aryani, Retno
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Physics Veterinary

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Hubungan Panjang Berat Dan Faktor Kondisi Relatif Lima Spesies Ikan di Sungai Suwi Muara Ancalong, Kutai Timur Nugroho, Rudy Agung; Florentino, Apri Pauci; Lariman, Lariman; Aryani, Retno; Rudianto, Rudianto; Kusneti, Monica
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 6, No 2 (2021): June 2021
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v6i2.3524

Abstract

The length-weight relationship of fish and the condition factor provides an overview of the fish growth conditions in the river. The purpose of this study was to determine the length-weight relationship and factor condition of five fish species in the Suwi River wetlands. The method of catching fish used gill nets, ceiling nets, fishing rods, traps, and other nets. The research was conducted for to months in the Suwi river wetlands. The results the length-weight relationship of five fish were varied, Ompok bimaculatus had b value 2,93, Helostoma temminckii (b  value 2.15), Mystus nemurus  (b value 2,62), and Channa) micropeltes (b 2.61) which indicated a negative allometric growth pattern. Meanwhile Leptobarbus hoevenii (b 3.12) was a positive allometric growth pattern. Negative allometric indicates growth in length faster than weight growth, thus the physical shape looks flattened, while positive allometric indicates growth in fish length slower than growth in fish weight. Based on the relative weight (Wr) of Ompok bimaculatus 101.28 ± 19.13, Helostoma temminckii 101.05 ± 15.51, Mystus nemurus 100.95 ± 14.84, Channa micropeltes 101.09 ± 14.14, Leptobarbus hoevenii 103.71 ± 34.57. Condition factor above 100, indicates sufficient food availability or low predator density
Plant Chromosome and Karyotype Analysis Training for High School/MA/Equivalent Teachers in Samarinda and its Surrounding Areas Oktavianingsih, Linda; Aryani, Retno; Hariani, Nova; Rosadi, Imam; Pratiwi, Anggita Endar; Arif, Muhammad Fauzi
Jurnal Pengabdian kepada Masyarakat (Indonesian Journal of Community Engagement) Vol 10, No 3 (2024): September
Publisher : Direktorat Pengabdian kepada Masyarakat Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpkm.88892

Abstract

Increasing biology teachers’ comprehension of the principles of genetics is attainable through the implementation of specialized training sessions focused on chromosome and karyotype analysis. In this training, 21 high school biology teachers from Samarinda and Nears participated in a structured training program encompassing three stages. In the initial phase, participants were provided with materials on the fundamental aspects of genetics. Subsequently, in the second phase, hands-on training was imparted, focusing on acquiring skills in plant chromosome preparation techniques. Lastly, the third stage concentrated on refining skills to construct chromosome karyotyping. Completing all stages was required for participants, followed by submitting evaluation forms for assessment by the committee. The evaluation outcomes revealed that 84.30% of participants regarded the presentation and assistance provided by principal lecturer and assistants as highly commendable. At the same time, the remaining respondents expressed satisfaction with the qualityand adequacy of the training. This comprehensive training effectively equipped participants with specialized chromosome and karyotype analysis skills, and the participants notably absorbed the training materials. The anticipated outcome of enhanced teacher understanding, specifically in the knowledge of inheritance and chromosome biology, was to stimulate heightened levels of student engagement and learning interest within the biology classroom.
The EFFECT OF COMBRETUM INDICUM LEAVES ON LIPID PROFILE AND HEMATOLOGY OF MICE INDUCED WITH ALLOXAN Rahayu, Sekar; Aryani, Retno; Rosadi, Imam; Nugroho, Rudy Agung
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol. 11 No. 1 (2024): Biolink Agustus
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v11i1.11441

Abstract

Diabetes has become one of the main causes of death throughout the world recent years. Diabetes symptoms include high blood glucose and hyperlipidemia. An rise in blood glucose can produce an increase in the amount of leukocytes and erythrocytes in the hematology profile. Consequently, this study was to ascertain how extract Combretum indicum leaves affected the lipid profile and hematological of mice given alloxan. The study sample included 36 male mice divided into six groups: normal control (KN) without treatment, negative control (K-) with alloxan treatment, positive control (K+) with alloxan and glibenclamide treatment, P1 with alloxan treatment and Combretum indicum extract dose 100 mg/kgBW, P2 with alloxan treatment and Combretum indicum extract dose 200 mg/kgBW, and P3 with alloxan treatment and Combretum indicum extract dose 400 mg/kgBW. After the mice hyperglycemia on day 3rd, the therapy was continued.  The treatment lasted for 20 days. On days 10 and 20, hematological and lipid profile measures were performed. Combretum indicum leaves have the ability to lower total cholesterol, LDL and triglyceride with ascalate HDL levels on 20 days of treatment. In addition, it markedly raised the percentage of lymphocytes and neutrophils with escalate the quantities of lymphocytes and neutrophils in alloxan induced mice. So, in aloxan-induced mice, this extract can reduce the effects of diabetes.
An IN VIVO EVALUATION OF COMBRETUM INDICUM FLOWER METHANOLIC EXTRACT AS OINTMENT FOR BURN WOUND ON MALE MICE Haroh, Istik; Aryani, Retno; Rosadi, Imam; Nugroho, Rudy Agung
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol. 11 No. 1 (2024): Biolink Agustus
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v11i1.11749

Abstract

Burns that are not treated properly can be dangerous, even can lead to death. Combretum indicum is reported to have anti-inflammatory, antibacterial, and high antioxidant activities, making it effective in healing dermal wounds. This study aimed to evaluate the wound healing effect of C. indicum flower methanolic extract on burn wound model in male mice. A total of 36 adult male mice were randomly assigned to 6 groups namely Negative control (I) without treatment; Positive Control I (II) with Burnazin treatment; Positive control II (III) with Vaseline treatment; Treatment group I (IV) with 25% concentration of C. indicum flower methanolic extract ointment; Treatment group II (V) with 50% concentration of C. indicum flower methanolic extract ointment; Treatment group III (VI) with 75% concentration of C. indicum flower methanolic extract ointment. Mice were treated daily for 20 days, and wound areas were observed every 2 days. Hydroxyproline level and total DNA content were tested on day-11. Skin tissue was analysed histologically. The results indicate that C. indicum flower extract accelerate wound healing process and repair the damaged skin tissue by accelerate the Proliferative phase.
Pengaruh Ekstrak Etanol Jahe Merah (Zingiber officinale) Terhadap Alanin Aminotransferase dan Aspartat Aminotransferase pada Serum Darah Mencit (Mus musculus) yang Diinduksi Asap Rokok Al Hasbi, Ezra; Aryani, Retno; Rosadi, Imam
BIOPROSPEK: Jurnal Ilmiah Biologi Vol 17 No 1 (2025): BIOPROSPEK: Jurnal Ilmiah Biologi
Publisher : Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/bp.v17i1.1234

Abstract

Asap rokok mengandung bahan kimia berbahaya bernama Reactive Oxygen Species (ROS), zat tersebut ialah agen radikal bebas yang menyebabkan darah kekurangan oksigen sehingga menimbulkan hipoksiadan menyebabkan nekrosis, sehingga melepaskan enzim transaminase ke peredaran darah. Tanaman obat yang umumnya digunakan oleh masyarakat Kalimantan adalah jahe merah (Zingiber officinale). Kandungan biokimia yang ditemukan pada jahe merah adalah Zingerone sebesar 25,8%, kemudian Zingiberene sebesar 15,9%. Tujuan dari penelitian ini ialah untuk mengetahui pengaruh pemberian ekstrak jahe merah terhadap kadar Alanin Aminotransferase (ALT) dan Aspartat Aminotransferase (AST) pada serum darah mencit (Mus musculus) serta berat badan mencit yang diinduksi asap rokok tersebut. Hasil penelitian yang didapatkan yaitu pemberian ekstrak etanol jahe merah tidak berpengaruh pada penurunan kadar ALT, tetapi berpengaruh signifikan pada penurunan kadar AST. Perubahan berat badan juga  berkorelasi dengan kadar ALT dan AST. Ekstrak etanol jahe merah dengan dosis 400mg/kgBB merupakan formulasi ekstrak yang paling efektif untuk menurunkan kadar AST dengan nilai sebesar 96 IU/l. Penelitian ini menunjukkan jika kadar berlebih pada suatu zat yang menyehatkan sekalipun dapat beresiko menimbulkan penyakit lain atau kegagalan dalam penyembuhan.
Effect of Water Hyacinth Leaf Flour (Eichhornia crassipes) Fermented by Aspergillus niger on the Growth, Survival Rate and Blood Profile of Sangkuriang Catfish (Clarias gariepinus) Ariyanto, Febri; Nugroho, Rudy Agung; Aryani, Retno; Manurung, Hetty; Rudianto, Rudianto
Aceh Journal of Animal Science Vol 8, No 2 (2023): June 2023
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/ajas.8.2.31390

Abstract

Catfish sangkuriang(Clarias gariepinus)is one of the aquaculture commodities with great potential to be developed in Indonesia to support the demand for feed. Efforts to reduce dependence on imported feed raw materials for fish feed is continued. Water hyacinth(Eichhornia crasspies)leaf which contains phytochemical compounds such as flavonoids, tannins,steroids and saponins is an alternatif raw material for fish pellet component. This study aimed to determine the addition of fermented water hyacinthleaf meal in fish feed on the growth, survival rate, and blood profile of sangkuriang catfish. In total 150 fish (Average initial weight 5.45 0.02 g)were randomly divided into triplicates of five groups, containing 10 fishes per group. Group one is a control group, P1 (10% of non fermented water hyacinth leaf meal), while P2, P3 and P4were treatment groups of fish fed with 10 , 15, and 20% fermented water hyacinth leaf meal byAspergillus niger. At the end of the 42 days of treatment, growth, survival rate, and blood profile of all fish were determined. The results showed that the addition of 10% water hyacinth leaf meal fermented byAspergillus nigerhad an effect on the growth of final weight, body weight gain and spesific growth rate (p 0.05) in comparison to all groups. However, the addition of any concentration of water hyacinth leaf meal did not affect on the feed conversion ratio and survival rate value. Further, the addition of water hyacinth leaf meal had a significant effect (p 0.05) on the erythrocytes, leukocytes and hemoglobin of fish, but did not show a significant effect on the values of hematocrit and platelets.
DIETARY CHITOSAN AND NANO-CHITOSAN OF BLACK SOLDIER FLY LARVAE FOR IMPROVING GROWTH AND PHYSIOLOGICAL INDICES OF Clarias gariepinus Mardianto, Nawwar; Aryani, Retno; Rudianto, Rudianto; Manurung, Hetty; Nugroho, Rudy Agung
Jurnal Riset Akuakultur Vol 20, No 2 (2025): Juni (2025)
Publisher : Politeknik Kelautan dan Perikanan Jembrana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jra.20.2.2025.103-119

Abstract

This research examined the effect of nutritional supplementation of 5% chitosan and nano-chitosan sourced from black soldier fly larvae exuviae on the growth and physiological profile of Clarias gariepinus (initial weight: 2.054 ± 0.02 g) over a period of 30 days. The feed treatments consisted of chitosan-supplemented, nano-chitosan-supplemented, and control feed, arranged in triplicate. In each trial unit, 30 fish were reared in plastic tanks (60 L capacity, filled with 50 L of freshwater). Growth, hematological profiles, and antioxidant activities were assessed after 30 days. The findings indicated that nano-chitosan markedly improved growth performance, as shown by increased final weight, body weight gain (BWG), and feed efficiency (FE), in comparison to chitosan and control diets. Specifically, nano-chitosan-fed fish exhibited a final weight of 2.878 ± 0.16 g ind-1 and a feed conversion ratio (FCR) of 1.376 ± 0.15, outperforming the chitosan group (2.660 ± 0.12 g ind-1; 1.267 ± 0.12) and the control group (2.344 ± 0.04 g ind-1; 1.857 ± 0.05). Additionally, nano-chitosan significantly increased activities of antioxidant enzymes, including those of superoxide dismutase (SOD) and catalase (CAT), while reducing malondialdehyde (MDA) levels, indicating reduced oxidative stress. The hematologicalprofile remained stable across the groups, confirming the safety of these feed additives. This research emphasizes nano-chitosan as a viable sustainable food additive for improving growth rate and oxidative resistance in C. gariepinus.  Penelitian ini mengevaluasi pengaruh suplementasi diet dengan 5% kitosan dan nano-kitosan dari cangkang pupa lalat tentara hitam terhadap pertumbuhan serta indeks fisiologis Clarias gariepinus (berat awal: 2,054 ± 0,02 g) selama 30 hari. Perlakuan pakan terdiri atas pakan yang disuplementasi nano-kitosan, disuplementasi kitosan, dan kontrol yang diulang tiga kali. Pada masing-masing unit percobaan, 30 ekor ikan dipelihara di wadah plastik (kapasitas 60 L, diisi dengan air sebanyak 50 L). Setelah 30 hari pemeliharaan, pertumbuhan, profil hematologi, dan aktivitas antioksidan dievaluasi. Hasil penelitian menunjukkan bahwa nano-kitosan meningkatkan kinerja pertumbuhan, yang ditunjukkan dengan peningkatan berat akhir, pertambahan bobot tubuh (PBT), dan efisiensi pakan (EP), dibanding dengan pakan kitosan dan kontrol. Secara khusus, ikan yang diberi pakan nano-kitosan menunjukkan bobot akhir 2,878 ± 0,16 g ekor-1 dan rasio konversi pakan (RKP) 1,376 ± 0,15, lebih tinggi dari kelompok kitosan (2,660 ± 0,12 g ekor-1; 1,267 ± 0,12) dan kelompok kontrol (2,344 ± 0,04 g ekor-1; 1,857 ± 0,05). Selain itu, nano-kitosan secara signifikan meningkatkan aktivitas enzim antioksidan, termasuk superoksida dismutase (SOD) dan katalase (CAT), sekaligus menurunkan kadar malondialdehida (MDA), yang mengindikasikan berkurangnya stres oksidatif. Parameter hematologi tetap stabil di seluruh kelompok, yang menegaskan keamanan bahan tambahan pakan ini. Penelitian ini menyoroti nano-kitosan sebagai suplemen pakan berkelanjutan yang menjanjikan untuk meningkatkan laju pertumbuhan dan ketahanan oksidatif pada C. gariepinus. 
EFFECT OF COMBRETUM INDICUM LEAF EXTRACT ON BLOOD SUGAR LEVELS AND PANCREAS HISTOLOGY OF ALLOXAN INDUCED MICE Pertiwi, Anggita Endar; Aryani, Retno; Rosadi, Imam; Nugroho, Rudy Agung
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol. 10 No. 2 (2024): February
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v10i2.11159

Abstract

Diabetes is a health problem that affects more than half a billion people in the world. Several plants are known to have anti-diabetic activity, one of which is Dutch jasmine (Combretum indicum). This research was conducted to determine the effect of Combretum indicum leaf extract on blood sugar levels and histology of islets of Langerhans in mice. A total of 36 male mice aged 6-8 weeks were divided into 6 treatment groups, namely normal control (KN) without treatment; negative control (K) with alloxan treatment; positive control (K+) with alloxan and glibenclamide treatment; P1 treated with alloxan and Combretum indicum leaf extract at a dose of 100 mg/kgBW; P2 with treatment with alloxan and Combretum indicum leaf extract at a dose of 200 mg/kgBW; P3 with alloxan treatment and Combretum indicum leaf extract at a dose of 200 mg/kgBW. Mice that have been induced by alloxan will be treated with leaf extract and glibenclamide for 20 days. Blood sugar levels 3 days after alloxan induction were checked and checked again on the 10th and 20th days of treatment by taking blood through the blood vessels in the mice's tails and then measuring them using a glucometer. The pancreas was dissected on the 20th day and pancreatic histology preparations were made for histological analysis. The results of this study indicate that Combretum indicum leaf extract has antihyperglycemic effects by protecting islets of Langerhans cells from alloxan-induced oxidative stress.
Nutritional Benefits of Black Soldier Fly Larvae Oil in Animal Feed Nugroho, Rudy Agung; Aryani, Retno; Hardi, Esti Handayani; Manurung, Hetty; Rudianto, Rudianto
Bioeksperimen: Jurnal Penelitian Biologi Vol 10, No 1: March 2024
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v10i1.23015

Abstract

Black Soldier Fly Larvae (BSFL) oil is currently an insect oil that potentially replaces fish oils in animal feed. However, there are limited information on its benefits and nutritional comparison of BSLF oil to fish oil. Present study was to evaluate the benefits of BSFL oil in animal feed and compare the BSFL to Fish oil. Collected data of BSFL oil in the animal feed and its physiological effects was obtained from several articles of  reputable journal and compiled into a table. Meanwhile, BSFL was reared and extract their oil to determine the fatty acid composition and compare to fish oil. The current study indicated that BSFL oil can be used in various animal feeds, such as aquafeed, broiler feed, and pets. The BSFL oil which is included in the feed showed an improvement in growth and other physiological responses of animals. Meanwhile, in comparison to fish oil, BSFL oil has a similar composition of fatty acids such as Omega 3 and Omega 6. Eicosapentaenoic acid (EPA; 20:5n3) is detected in the BSFL oil, though the quantity is much lower than in fish oil.  Further, the BSFL oil also contains lauric acid. In conclusion, BSFL oil can be an alternative feed ingredient to substitute fish oil and has beneficial to the growth and physiological responses of the animal.
PENGARUH EKSTRAK DAUN RAMBUTAN (Nephelium lappaceum L.) SEBAGAI BAHAN SALEP TERHADAP PERCEPATAN PENUTUPAN LUKA SAYAT, KADAR HIDROKSIPROLIN DAN TOTAL DNA MENCIT (Mus musculus L.) Naimah, Wa; Aryani, Retno; Nugroho, Rudy Agung
BIOPROSPEK: Jurnal Ilmiah Biologi Vol 17 No 2 (2025): BIOPROSPEK: Jurnal Ilmiah Biologi
Publisher : Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/c2br7242

Abstract

Daun Rambutan (Nephelium lappaceum L.) mengandung metabolit sekunder seperti flavonoid, tanin, dan saponin. Kandungan metabolit sekunder tersebut dapat membantu pembentukan kolagen sehingga mempercepat penyembuhan luka. Tujuan dalam penelitian ini yaitu untuk mengetahui pengaruh ekstrak metanol daun rambutan sebagai bahan salep dengan parameter terhadap persentase penutupan luka sayat, kadar hidroksiprolin, dan kandungan total DNA pada mencit. Penelitian ini menggunakan rancangan acak lengkap (RAL) dengan 2 kelompok, yang terdiri dari 2 kelompok kontrol (K+: Povidone Iodine 10% dan K-: Tanpa Perlakuan) dan 3 kelompok perlakuan (P1: Salep ekstrak daun rambutan 5%, P2: Salep ekstrak daun rambutan 10%, dan P3: Salep ekstrak daun rambutan 15%) dengan 5 kali pengulangan, menggunakan sebanyak 25 ekor mencit jantan. Mencit diberikan luka sayat pada punggungnya dan diberi perlakuan sesuai kelompok selama 16 hari. Pengukuran panjang luka pada mencit dilakukan setiap 2 hari sekali. Pengambilan jaringan kulit mencit dilakukan pada hari ke-11 setelah luka, kemudian jaringan kulit mencit diukur kadar hidroksiprolin dan total DNA-nya. Hasil yang didapatkan menunjukkan bahwa salep dengan konsentrasi 15% lebih cepat dalam penyembuhan luka dilihat dari persentase penutupan luka.
Co-Authors Abd. Rasyid Syamsuri Al Hasbi, Ezra Ariyanto, Febri Esti Handayani Hardi Florentino, Apri Pauci Haroh, Istik Hetty Manurung, Hetty Imam Rosadi Kusneti, Monica Lariman, Lariman Linda Oktavianingsih Mardianto, Nawwar Muhammad Fauzi Arif Naimah, Wa Nova Hariani Pertiwi, Anggita Endar Pratiwi, Anggita Endar Rahayu, Sekar Rudianto Rudianto Rudy Agung Nugroho