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All Journal HAYATI Journal of Biosciences Jurnal Hortikultura Indonesia (JHI) AL KAUNIYAH Jurnal Penelitian Tanaman Industri Indonesian Journal of Biotechnology Jurnal Sumberdaya Hayati JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Bioteknologi & Biosains Indonesia (JBBI) ANNALES BOGORIENSES Journal of Microbial Systematics and Biotechnology Jurnal Penelitian Tanaman Industri (Littri) Berkala Penelitian Hayati Makara Journal of Science Annales Bogorienses
Aris Tjahjoleksono
Departemen Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor. Jl. Agathis, Kampus IPB Darmaga, Bogor 16680, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology

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Journal : Makara Journal of Science

 1 
Identification of Drought-tolerant Local Cowpea Varieties of Southwest Maluku (Indonesia) Karuwal, Ritha Lusian; Suharsono, Suharsono; Tjahjoleksono, Aris; Hanif, Novriyandi
Makara Journal of Science Vol. 22, No. 4
Publisher : UI Scholars Hub

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Abstract

Cowpeas grown in Southwest Maluku have local potential due to their diversity and ability to adapt to drought stress conditions that otherwise cause low productivity. The purpose of this study was to identify of local cowpea varieties of Southwest Maluku that show tolerance to drought. The experimental samples were seven local varieties and threereference cultivars. The drought stress treatment was altered watering periods, i.e., every two (P0) and ten days (P1) from growth to harvest. The results of this research showed that a ten-day watering period significantly decreased plant height, number of leaves, number of root nodules, root and shoot dry weight, photosynthesis rate, stomatal conductance, transpiration rate, relative water content, media water content, chlorophyll content, and number and weight of seeds perplant. Drought stress treatment increased proline content and root length. Correlation analysis showed a significant correlation between plant height with all variables, except for root length, proline content, and weight of seedsper plant. The correlation and cluster analyses showed that the KM7 variety is a drought tolerant genotype among the local cowpea varieties from Southwest Maluku. Therefore, the KM7 variety can be used as plant material in future breeding programs.
Transformation of Inhibitor of Meristem Activity (IMA) Gene into Jatropha curcas L. Paserang, Asri Pirade; Tjahjoleksono, Aris; Widyastuti, Utut; Suharsono,
Makara Journal of Science Vol. 19, No. 3
Publisher : UI Scholars Hub

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Jatropha is one of the many biodiesel plants developed in tropical countries. Efforts to increase its productivity can be done using various methods of breeding. One of the breeding methods is the introduction of genes into the Jatropha plant. The aim of this study is to assess the success of genetic transformation using the Inhibitor of Meristem Activity (IMA) gene in Jatropha curcas. The research procedures included inoculation of explants with Agrobacterium tumefaciens, callus induction, screening test of selection media, regeneration, and gene expression analysis using Polymerase Chain Reaction (PCR). IMA is one of the genes that controls flowering genes and ovule development. It was first isolated from tomato plants and has been successfully overexpressed in these plants using the Cauliflower Mosaic Virus (CaMV) 35S promoter. In this experiment, plant transformation was performed on J. curcas as the target. Explant callus formation in both the control and treated samples was good, but shoot formation decreased dramatically in the treated explants. PCR analysis indicated that IMA genes can be inserted into J. curcas with the size of the IMA gene is 500 bp.
Co-Authors . SUHARSONO Aas Ratnasari Ai Nurhasanah Husnul Izzati Antonius Suwanto Aris Tri Wahyudi Asri Pirade Paserang Diah Ratnadewi Dwinita Wikan Utami Edi Husen Fatimah, Anggi Siti Febrina Ariyanti Ismail Fitri Damayanti Gustania, Leolita Hamim Hamim HIASINTA F. J. MOTULO Idha Susanti Idha Susanti Ika Mariska Ismi Isti'anah Karuwal, Ritha Lusian Mahfudhah, Farah MEITY S. SINAGA Miftahudin Miftahudin . Miftahudin Miftahudin Miftahudin, M Miftahudin, M. Muhammad Faiz Amri Musawira Musawira Musawira MUTTAQIN, MAFRIKHUL N Sri Hartati NISA RACHMANIA MUBARIK Novriyandi Hanif Nurhasanah, Eka Ovi Prasetya Winandari Pasmawati Pasmawati Ritha Lusian Karuwal ROHANI CINTA BADIA GINTING SIENTJE MANDANG Suaidi, Malik Nuris Suharsono Suharsono Suharsono Suharsono Suharsono Suharsono Suharsono Suharsono Suharsono Suharsono Suharsono, Suharsono, Suharsono Theowidavitya , Brian Ulfah Juniarti Siregar Utut Widyastuti Utut Widyastuti UTUT WIDYASTUTI Utut Widyastuti Windarti Wahyuningtyas