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Elderly nutritional status effection salivary anticandidal capacity against Candida albicans Ria Puspitawati; Nurtami Soedarsono; Elisabeth A Putri; Anissha D Putri; Boy M Bachtiar
Dental Journal (Majalah Kedokteran Gigi) Vol. 44 No. 2 (2011): June 2011
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (349.566 KB) | DOI: 10.20473/j.djmkg.v44.i2.p72-76

Abstract

Background: Elderly often suffer malnutrition and oral candidiasis. Candida albicans (C. albicans) which is the most prominent cause of oral candidiasis, is one of commensal oral micro-flora. Nutritional status affect the characteristic of saliva. Saliva is the regulator in the development of C. albicans from comensal into pathogen. Purpose: The purpose of this study was to determining the correlation between elderly nutritional status with salivary total protein and its activity in inhibiting C. albicans growth and biofilm formation. Methods: Using mini nutritional assessment 30 elderly were classified into normal and malnutrition groups. Total protein of unstimulated saliva was measured using Bradford protein assay. The colony forming unit (CFU) of C. albicans was counted on 72 hours on SDA cultures without (control) or with 2 hour saliva exposure. Biofilm formation was analyzed from the optical density of 10–5 C. albicans suspension without saliva exposure (control) or with exposure of 10.000 μg/ml saliva and incubated in 37° C for 2 days. The suspension was put into 96 well plates, stained with crystal-violet dye, and analyzed using microplate reader. Differences between groups were analyzed using independent t-test or Kruskall-Wallis. Correlation between variables was analyzed using Spearman test. Results: Salivary total protein of normal elderly (1.113.5 ± 1.1143.3) was higher than those of malnutrition (613.6 ± 253.6) but not statistically significant (p > 0.05). The CFU of C. albicans exposed to saliva of normal samples (2.060 cfu/ml) was significantly lower than control (24.100 cfu/ml) and those exposed to malnutrition saliva (5.513.3 cfu/ml). C. albicans biofilm formation is highest in controls (0.177), lower in those exposed to malnourished saliva (0.151) and lowest in those exposed to saliva of good nourished elderly (0.133). Conclusion: Although does not cause significant decrease of salivary total protein, malnutrition in elderly results in lower capacity of saliva in inhibiting the growth and declining the virulence of C. albicans.Latar belakang: Lansia sering menderita malnutrisi dan kandidiasis oral. Candida albicans yang merupakan penyebab utama terjadinya candidiasis, adalah salah satu mikroflora rongga mulut yang bersifat konvensional. Malnutrisi memengaruhi karakteristik saliva. Saliva merupakan regulator utama perkembangan Candida albicans (C. albicans) dari sifat konvensional menjadi bersifat patogen.Tujuan: Tujuan dari penelitian ini adalah untuk menentukan korelasi antara status gizi lansia dengan total protein dan aktivitas saliva dalam menghambat pertumbuhan dan pembentukan biofilm C. albicans. Metode: Menggunakan mini nutritional assessment, 30 lansia diklasifikasikan menjadi kelompok gizi baik dan gizi buruk. Total protein unstimulated saliva diukur dengan metode Bradford protein assay. Colony forming unit (CFU) dihitung pada kultur C. albicans pada saburaud dextrose agar (SDA) berusia 72 jam yang sebelumnya telah dipaparkan saliva selama 2 jam. Kontrol adalah kultur C. albicans tanpa paparan saliva. Pembentukan biofilm adalah pengukuran optical density suspensi 10–5 C. albicans tanpa paparan saliva (kontrol) atau dengan paparan saliva 10.000 μg/ml dan diinkubasi pada suhu 37° C selama 2 hari. Suspensi tersebut kemudian dimasukkan ke dalam 96 well plates, diberi pewarna crystal violet, dan diukur menggunakan microplate reader. Analisis data menggunakan uji beda t Independen atau Kruskall-Wallis, dan uji korelasi Spearman. Hasil: Total protein saliva lansia gizi baik (1.113,5 ± 1.1143,3) lebih tinggi dari lansia gizi buruk (613,6 ± 253,6) tetapi tidak bermakna secara statistik (p > 0,05). Pembentukan koloni C. albicans yang terpapar saliva lansia gizi baik (2.060 cfu/ml) secara signifikan lebih rendah dari kontrol (24.100 cfu/ml) dan daripada yang terpapar saliva lansia gizi buruk (5.513,3 cfu/ml). Pembentukan biofilm C. albicans tetinggi pada kontrol (0,177), lebih rendah pada yang terpapar saliva gizi buruk (0,151) dan terrendah pada yang terpapar saliva gizi baik (0,133). Kesimpulan: Meskipun malnutrisi tidak menyebabkan penurunan total protein saliva lansia, tetapi menurunkan kapasitasnya dalam menghambat pertumbuhan dan mengurangi virulensi C. albicans.
Kolumna Vertebral sebagai Parameter Estimasi Usia dan Determinasi Jenis Kelamin Roben S. Pasaribu; Ria Puspitawati; Ayu Rahmadhani
Medical Scope Journal Vol. 5 No. 2 (2023): Medical Scope Journal
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/msj.v5i2.49892

Abstract

Abstract: Age estimation and sex determination using bone parameters have high accuracy. Vertebral column is a group of five types of bones; each type has specific characteristics, shape, and properties. The unique and specific characteristics of each type of vertebra differ between sexes and change with age. This phenomenon makes the vertebral column as a potential parameter for age estimation and sex determination. This was a literature review study aimed to discuss about using vertebral column as the parameter of age estimation and sex determination. The results showed that age estimation using vertebral column parameters by observing osteophyte formation had a correlation of r=0.7 and p<0.01. Maturation of the vertebral column and age showed a correlation of r=0.695 and p<0.001. Age estimation by observing the vertebral column maturation in the age group 15-22 years showed 95% accuracy. Sex determination using vertebral column parameters showed high accuracy, reaching 93% in the lumbar vertebrae and 86-89.7% in the cervical vertebrae. In conclusion, vertebral column has great potential in the forensic investigation since it can be used as a parameter for age estimation and sex determination. Keywords: vertebral column; age; sex; bone maturation; osteophyte; forensic identification   Abstrak: Estimasi usia dan determinasi jenis kelamin dengan menggunakan parameter tulang memiliki akurasi yang baik. Kolumna vertebral tersusun dari lima kelompok jenis tulang yang memiliki ciri, bentuk, dan karakteristik yang spesifik. Karakteristik unik dan spesifik dari masing-masing jenis tulang vertebra dapat menunjukkan perbedaan antar jenis kelamin dan perubahan seiring bertambahnya usia. Fenomena demikian menjadikan kolumna vertebral berpotensi sebagai parameter dalam estimasi usia dan determinasi jenis kelamin. Studi ini merupakan suatu literature review yang bertujuan untuk mengetahui penggunaan  kolumna vertebral sebagai parameter estimasi usia dan determinasi jenis kelamin. Hasil studi mendapatkan estimasi usia menggunakan parameter kolumna vertebral dengan melihat pembentukan osteofit menunjukkan korelasi r=0,7 dan signifikansi p<0,01. Maturasi kolumna vertebral dan usia menunjukkan korelasi r=0,695 dan p<0,001. Estimasi usia dengan melihat maturasi kolumna vertebral pada rentang usia 15-22 tahun memberikan akurasi mencapai 95%. Determinasi jenis kelamin menggunakan parameter kolumna vertebral menunjukkan akurasi tinggi mencapai 93% pada kolumna vetebral bagian lumbar dan 86-89,7% pada bagian servikal. Simpulan studi ini ialah kolumna vertebral merupakan salah satu jenis tulang yang memiliki potensi besar dalam dunia forensik untuk dijadikan sebagai parameter estimasi usia dan determinasi jenis kelamin. Kata kunci: kolumna vertebral; usia; jenis kelamin; maturasi tulang; osteofit; identifikasi forensik
Dental Profiling and Findings of Multiple Jaw Fractures in Traffic Accident Victim: A Case Report Yunus, Aditia D.; Gultom, Ferry P.; Puspitawati, Ria; Sari, Fitri A.; Prastyo, Eko; Rijaldi, Ferdy; Suripto, Suripto
e-GiGi Vol. 12 No. 2 (2024): e-GiGi
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/eg.v12i2.53455

Abstract

Abstract: Motorcycle riders have a high prevalence of accident risks. These accidents can result in severe injuries and can even lead to death. The death prevalence among motorcyclists is three times higher than that of car passengers, six times higher than that of pedestrians, and almost 50 times higher than that of bus occupants. Traffic accidents can cause blunt trauma. High-speed crashes are common in polytraumatized motorcycle crashes and often display facial injuries. In this case report, the authors present a case of an accident with findings of facial trauma to the forehead, nose, cheeks, and multiple fractures of the maxilla and mandible due to a hard object impact. Dental examination revealed post-mortem tooth loss, fractures of the teeth, attrition, root remains, and visible impaction. In conclusion, craniofacial blunt trauma presents with many clinical features. The authors recommend that the finding of such cases be performed further examinations such as X-rays to gain an idea of the possible location of the fracture and to do an autopsy. Keywords: motorcycle accident; blunt trauma; maxillary fracture; mandibular fracture
Age Estimation via Orocraniofacial Based on Direct Observation and Radiography Asdiana, Andi Eka; Puspitawati, Ria; Soedarsono, Nurtami
Journal La Medihealtico Vol. 5 No. 1 (2024): Journal La Medihealtico
Publisher : Newinera Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37899/journallamedihealtico.v5i1.1036

Abstract

Determining the estimated age in forensics is very important because it involves legal accountability. The method of determining age estimation can be done by direct measurement of the orocraniofacial bone or by using radiography. This article examines theseveral studies as well as parameters for determining age estimation using orocraniofacial. Search for published articles on Elsevier, Pubmed, Google Scholar, Research Gate, and NCBIon range2007-2021 years. The results show that it is more advisable to determine the age estimate by direct measurement compared to using radiography because it is prone to distortion.
Morphological and Morphometric Analysis of Orocraniofacial Bones for Estimative Identification Gender Sari, Wulan Purnama; Soedarsono, Nurtami; Puspitawati, Ria
Journal La Lifesci Vol. 5 No. 1 (2024): Journal La Lifesci
Publisher : Newinera Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37899/journallalifesci.v5i1.1172

Abstract

Identification of sex estimation is the first step in the identification process and is one of the priority parameters in the forensic identification process. Identification is made when the condition of the body is unrecognizable due to natural disasters, transport accidents, bomb explosions, ethnic studies and crime investigations, where only a few parts of the body are already incomplete and limited information are available. In forensic odontology, morphological and morphometric analysis of the orocraniofacial bone is an important component that can help in the process of identifying sex estimation because it has different shapes and sizes in each individual and population. This article will discuss morphological and morphometric analysis of several parameters of the orocraniofacial bone, as well as the advantages and limitations of both methods.
Characteristics of Patchouli Plant (Pogostemon cablin Benth.) as An Antimicrobial in The Development of Dental Materials Deviyanti Pratiwi; Decky Joesiana Indrani; Sunarso Sunarso; Ria Puspitawati
Interdental Jurnal Kedokteran Gigi (IJKG) Vol. 21 No. 1 (2025): Interdental Jurnal Kedokteran Gigi (IJKG)
Publisher : Fakultas Kedokteran Gigi, Universitas Mahasaraswati Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46862/interdental.v21i1.10572

Abstract

Introduction: Various natural ingredients have been developed in health science to avoid side effects that arise due to the use of chemicals. Pogostemon cablin Benth, or the patchouli plant, is a natural ingredient that has been researched and has potential as an antimicrobial and antioxidant in health problems. The development of natural materials in dentistry is influenced by their physical, chemical, and biological properties. This review aims to determine the various characteristic tests that need to be carried out to support the potential development of Pogostemon cablin Benth as an antimicrobial material in dentistry. Review: All parts of the patchouli plant can be used in the form of extracts and essential oils that contain secondary metabolites and other active ingredients, Patchouli Alcohol and its derivatives, which have an effect on antimicrobial activity. Another form of utilization of Pogostemon cablin Benth., such as emulsions, nanohydrogels, and encapsulations, aimed at increasing their activity. Various tests related to physical, chemical, and biological characteristics can be carried out to optimize the content of Pogostemon cablin Benth. so that it can be used appropriately, especially regarding health problems. Conclusion: The results of this search have provided an overview of Pogostemon cablin Benth, which has physical, chemical, and biological properties that have the potential to become antimicrobial materials for dentistry.
Three-dimensional analysis of nasolabial fold depth as a support for facial reconstruction methods for corpse identification: A literature review Apriani, Regina Carsa Bagin; Puspitawati, Ria; Gultom, Ferry Pergamus; Soedarsono, Nurtamy; Yuniastuti, Mindya
Science Midwifery Vol 12 No 2 (2024): June: Health Sciences and related fields
Publisher : Institute of Computer Science (IOCS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35335/midwifery.v12i2.1533

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Identification of human remains is one of the most important stages in forensic science among the various methods commonly used in the process of identifying unknown human remains. Identification of remains is an issue in both criminal and civil cases. Determining the correct identity of the body is very important in the investigation as a mistake could have fatal consequences. Facial reconstruction is a method that is developing rapidly. The aim of this review is to acknowledge the importance of the nasolabial fold as one of the parameters required for identification using facial reconstruction methods. The depth of soft tissue is important in facial reconstruction. Aging of the face is associated with loss of soft tissue support in the nasolabial sulcus. The nasolabial sulcus is made up of both soft and hard tissue. The depth of the nasolabial sulcus can affect facial expression. 3D computerized facial reconstruction has been developed for a long time, but still requires continuous development using approaches to reconstruct the face. The nasolabial sulcus influences facial expression in facial reconstruction. Technological advances improve or facilitate the work of forensic odontologists, anthropologists, and dentists compared to traditional methods.
DENTAL AGE ESTIMATION FROM TOOTH DEVELOPMENT: A LITERATURE REVIEW Nur Azizah, Anna; Dedek Yunus, Aditia; Puspitawati, Ria; Soedarsono, Nurtami; Winoto Suhartono, A.
Journal of Health and Dental Sciences Vol. 3 No. 3 (2024): Journal of Health and Dental Sciences
Publisher : Fakultas Kedokteran Gigi Unjani

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Age estimation is among several standard methods for identifying unknown individuals in disasters, accidents, crime victims, and identity forgery. Age estimation can be done through observation of the growth and development of dentition. Similar to fingerprints, teeth are unique and specific for each individual. This article discusses the methods of age estimation based on observation of dentition's growth and development process. Demirjian, Gustafson, Kvaal, Gleiser and Hunt, Schour and Massler, and Cameriere are the most commonly used age estimation methods. Each method has its challenges and limitations. Therefore, applying more than one method or conducting a repetition of measurement and calculation could improve the accuracy of the estimation. However, the influence of factors like heredity, ethnicity, and genetic tendencies in a particular population should also be considered. DOI : 10.54052/jhds.v3n3.p249-262
ANALISIS FRAMING KOMPAS.COM ATAS TREN BELI EMAS SEBAGAI DAMPAK KEBIJAKAN EKONOMI DONALD TRUMP Ria Puspitawati; Nik Haryanti; Endah Siswati
Jurnal Media Akademik (JMA) Vol. 3 No. 9 (2025): JURNAL MEDIA AKADEMIK Edisi September
Publisher : PT. Media Akademik Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62281/7bmpdg42

Abstract

Perkembangan ekonomi global yang penuh ketidakpastian, salah satunya akibat kebijakan ekonomi Presiden Amerika Serikat Donald Trump, mendorong masyarakat beralih pada emas sebagai investasi aman (save haven). Penelitian ini bertujuan untuk mengetahui bagaimana Kompas.com membingkai pemberitaan tren beli emas sebagai respons terhadap kondisi global. Penelitian menggunakan pendekatan kualitatif deskriptif dengan metode analisis framing Robert N. Entman, yang meliputi empat perangkat : Pendefisian masalah, diagnosis penyebab, penilaian moral, dan rekomendasi solusi. Hasil penelitian menunjukkan bahwa Kompas.com membingkai kebijakan Donald Trump sebagai pemicu ketidakstabilan ekonomi global yang beujung pada lonjakan harga emas. Emas diposisikan sebagai investasi aman, sementara fenomena Fear of Missing Out (FOMO) masyarakat Indonesia dibingkai secara positif sebagai bentuk kewaspadaan finansial. Selain itu, berdasarkan teori efek media Steven M. Chaffee, framing berita Kompas.com berpengaruh terhadap khalayak pada tiga ranah : (1) Kognitif : menambah pemahaman tentang hubungan kebijakan global dengan harga emas, (2) Afektif : membangun keyakinan positif terhadap emas sebagai investasi, dan (3) Behavioral : mendorong tindakan nyata berupa pembelian emas secara rasional Dengan demikian, Kompas.com tidak hanya berperan sebagai penyampai informasi, tetapi juga sebagai agen edukasi yang mendorong masyarakat untuk adaptif, rasional dan bertanggungjawab dalam pengambilan keputusan investasi.