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Immunoglobulin-Y Effect On Protein Of Streptococcus Mutans Isolated From Caries And Caries-Free Subjects Aditiya Irwandi, Rizky; Winiati Bachtiar, Endang; Yuniastuti, Mindya
Insisiva Dental Journal Vol 1, No 1 (2012)
Publisher : Insisiva Dental Journal

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The main microbial culprit in dental caries is Streptococcus mutans (S.mutans),virulence of which can be observed by its differential protein expression betweencaries and caries-free subjects.The success of Immunoglobulin-Y (IgY) antiS.mutans as a passive immunization agent in eliminating S.mutans has beenreported. The aim of this study is to analyze the effect of IgY anti S.mutans on theprotein expression of S.mutans isolated from caries and caries-free subjects. Eachdental plaque was collected by swabbing the buccal surface of the first lowerpermanent molar of caries and caries-free subjects. The plaques were thencultured on agar medium TYS20B. After 72 hours, the colonies from each of themwere cultured in liquid medium TYS Broth for 72 hour. Each collected bacteria(whether from caries or caries-free subjects) were grouped into control andexposure group. In exposure group, S.mutans was exposed by pre-incubated (forone hour at 37Â°C) IgY anti S.mutans for one hour at 37Â°C. Protein expression ofS.mutans was analyzed with SDS PAGE after the preparation of its antigen andBradford protein assay. Our result shows that S.mutans 41.3 kilodalton proteinexpression of caries subjects, are up-regulated in comparison to the control group.Meanwhile, the S.mutans 41.3 kilodalton protein expression of caries-freesubjects, are down-regulated in comparison to the control group. This studysuggests that IgY anti S.mutans up-regulates 41.3 kilodalton protein expression ofS.mutans in the caries subjects. However IgY anti S.mutans down-regulates 4.13kilodalton protein expression of S.mutans in the caries-free subjects.

PENGGUNAAN RADIOGRAF GIGI UNTUK KEPENTINGAN IDENTIFIKASI FORENSIK Sita Rose Nandiasa; Bramma Kiswanjaya; Mindya Yuniastuti
ODONTO : Dental Journal Vol 3, No 1 (2016): July 2016
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.3.1.74-77

Background: Tooth has a unique characteristic. Teeth often used for forensic identification especially when the other parts of the body could not gave a difference feature used for identification.Discussion: Tooth is part of the body known for its resistant against external influence making it as an important primary identifier. Dental radiographic can act as a helpful tool in identification process to compare antemortem and postmortem data. Radiograph can give a detail features from the teeth and their surroundings, especially digital radiograph so it can ease the identification process.Conclusion : forensic identification using radiograph can be trusted.

Oral Clinical Findings in Cadaver with Tuberculosis: A Case Report Azizah, Anna; Wahyu, Nadilla I.; Rahmadhani, Ayu; Soedarsono, Nurtami; Yuniastuti, Mindya; Suhartono, Antonius W.; Syarifudin, Mohammad A.; Fonataba, Anthon G.
e-GiGi Vol. 13 No. 1 (2025): e-GiGi
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/eg.v13i1.54414

Abstract: Tuberculosis (TB) is a long-lasting infectious disease that usually affects the lungs (pulmonary TB), but can also affect other organs and systems (extra-pulmonary TB). Oral TB is uncommon, particularly primary oral tuberculosis which predominantly affects young adults, and the tongue is the site most frequently affected. This case report aimed to discuss the manifestation of TB in the hard palate, focusing on observation from a cadaver and providing a thorough analysis of the oral clinical symptoms. We reported a case of unidentified 42-year-old female body that was found dead and brought to Dr. Cipto Mangunkusumo Hospital for an autopsy. Poor oral hygiene was found. In the palate, there was bone defect on palatum durum regio teeth 21 22 near to the incisive foramen. Pink teeth was discovered in the anterior part of the lower jaw. Based on the autopsy report, the cause of death was tuberculosis. In conclusion, although tuberculosis of the palate is infrequent, it should be included as a potential cause of palatal perforation during diagnostic evaluations. Additionally, it is crucial to conduct a comprehensive investigation into the primary site of tuberculosis in cases involving palatal tuberculosis. Keywords: oral tuberculosis; palatal perforation; pink teeth phenomenon

Effectiveness of the Al Qahtani method in estimating gestational age in the context of an alleged illegal abortion : a case report Rijaldi, Ferdy; Yudy, Yudy; Prastyo, Eko; Soedarsono, Nurtami; Yuniastuti, Mindya; Satriyo, Juanda; Auerkari, Elza Ibrahim
Padjadjaran Journal of Dentistry Vol 37, No 1 (2025): April 2025 (Supplements 1)
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol37no1.58832

Introduction: Age estimation is crucial in forensic and dental sciences, particularly for determining gestational age in suspected illegal abortion cases. This study highlights the significance of the Al Qahtani method, which focuses on dental development, as a reliable approach for estimating gestational age. Gestational age is commonly assessed through body length, weight, and the maturation of skeletal and dental structures, with radiographs serving as essential tools for evaluating dental development. The aim of this case report is to demonstrate the effectiveness of the Al Qahtani method in estimating gestational age in the context of an alleged illegal abortion. Case report: The body of an unidentified infant was found on the side of the road, wrapped in a piece of white cloth and a brown sarung. It was transported to the hospital’s forensic department for identification. Periapical radiographs were taken of the anterior areas of the teeth, and the Al Qahtani method was employed to determine the age of the deceased infant. The radiographs provided a clear overview of dental maturation, which was essential for accurate age estimation. Conclusion: The Al Qahtani method is recommended as a standard practice in forensic odontology for estimating gestational age in abortion cases. This method contributes significantly to medical treatment, criminal investigations, and legal proceedings, enhancing the accuracy of age determination and supporting both medical and legal contexts.

Trismus Analysis Due to Head Trauma in Forensic Cases: Case Report Langit, Ken Sekar; Kaurow, Farah Primadani; Yuniastuti, Mindya; Aeurkari, Elza Ibrahim
Odonto : Dental Journal Vol 12, No 1 (2025): April 2025
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.12.1.97-102

Head trauma frequently happens in cases of physical violence, particularly in cases of abuse. A common sign of head trauma is trismus, characterized by difficulties in mouth opening, which can diminish an individual's quality of life and disrupt daily activities. The prognosis of trismus is contingent upon various factors, necessitating appropriate management in its therapy. The age factor and the severity of the etiology of trismus must be evaluated to determine the appropriate treatment. This condition also influences the decision-making over the classification of the wound documented in the Visum et Repertum for judicial purposes.

Trismus Analysis Due to Head Trauma in Forensic Cases: Case Report Langit, Ken Sekar; Kaurow, Farah Primadani; Yuniastuti, Mindya; Aeurkari, Elza Ibrahim
Odonto : Dental Journal Vol 12, No 1 (2025): April 2025
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.12.1.97-102

Head trauma frequently happens in cases of physical violence, particularly in cases of abuse. A common sign of head trauma is trismus, characterized by difficulties in mouth opening, which can diminish an individual's quality of life and disrupt daily activities. The prognosis of trismus is contingent upon various factors, necessitating appropriate management in its therapy. The age factor and the severity of the etiology of trismus must be evaluated to determine the appropriate treatment. This condition also influences the decision-making over the classification of the wound documented in the Visum et Repertum for judicial purposes.

The role of forensic odontology in the identification of a mutilation victim: A case report Yatma, Debby; Auerkari, Elza Ibrahim; Yuniastuti, Mindya; Soedarsono, Nurtami; Suhartono, Antonius Winoto; Kaurow, Farah P.; Pasaribu, Roben Suhadi; Auerkari, Pertti
Dental Journal (Majalah Kedokteran Gigi) Vol. 58 No. 4 (2025): December
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v58.i4.p409-414

Background: Forensic odontology is an invaluable resource for human identification. An individual’s bones and teeth may be the only remnants of their identity in certain situations where soft tissue has been lost, carbonized, or destroyed for any other reason. The body’s hardest and best-protected structures are found in teeth, and these have individual characteristics and are resistant to factors such as temperature and chemistry. When antemortem dental records are not available for comparison, dental profiling is performed. Purpose: This case study highlights the importance of teeth in the identification process and their usefulness in estimating biological profile factors such as sex, age, and population affinity. Case: The case of an unidentified woman who was found in a house in a severely decomposed, skeletonized state is presented. The unidentified body was sent to the Forensic Medicine Installation of Bhayangkara Level I Hospital to determine the cause of death and for dental identification. Case management: The body was suspected to be a victim of mutilation. Shovel shapes observed on the lingual surface of the lateral upper incisor and tori palatini are the most outstanding diagnostic features in Mongoloid populations. The specific mesiodistal crown width and mandibular canine index revealed female traits. Using the Lamendin method, the age was estimated to be 52–58 years old. Conclusion: A forensic odontologist can identify individuals by comparing antemortem and postmortem dental data. They can also provide age estimation, sex, and population affinity determination by analyzing teeth.

Teknik reseksi mandibula pada autopsi Tanjung, Rizki; Soedarsono, Nurtami; Yuniastuti, Mindya; Ibrahim Auerkari, Elza
Jurnal Kedokteran Gigi Terpadu Vol. 5 No. 2 (2023): Jurnal Kedokteran Gigi Terpadu
Publisher : Fakultas Kedokteran Gigi Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25105/jkgt.v5i2.18854

Background: Jaw resection in the forensic autopsy has a different purpose rather than the usual goals such as removal of the tumor, fracture repair or reducing osteomyelitis. However, the purpose of forensic autopsies is actually to identify the remains of victims who cannot be identified with fingerprints or DNA conventionally or by means of identifying unidentified victims. Objective: This study aims to review jaw resection techniques at autopsy. Method: Scoping review is carried out by analyzing secondary data in the form of journals published from 2008-2021. Results: There were five journals used in this study. The five journals show that the jaw resection procedure starts from an incision first and then a cut is made to the maxilla or mandible. Conclusion: Jaw resection at autopsy is needed for sufficient visibility. It is usually reserved for cases of severely rotting or burned body parts.

Three-dimensional analysis of nasolabial fold depth as a support for facial reconstruction methods for corpse identification: A literature review Apriani, Regina Carsa Bagin; Puspitawati, Ria; Gultom, Ferry Pergamus; Soedarsono, Nurtamy; Yuniastuti, Mindya
Science Midwifery Vol 12 No 2 (2024): June: Health Sciences and related fields
Publisher : Institute of Computer Science (IOCS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35335/midwifery.v12i2.1533

Identification of human remains is one of the most important stages in forensic science among the various methods commonly used in the process of identifying unknown human remains. Identification of remains is an issue in both criminal and civil cases. Determining the correct identity of the body is very important in the investigation as a mistake could have fatal consequences. Facial reconstruction is a method that is developing rapidly. The aim of this review is to acknowledge the importance of the nasolabial fold as one of the parameters required for identification using facial reconstruction methods. The depth of soft tissue is important in facial reconstruction. Aging of the face is associated with loss of soft tissue support in the nasolabial sulcus. The nasolabial sulcus is made up of both soft and hard tissue. The depth of the nasolabial sulcus can affect facial expression. 3D computerized facial reconstruction has been developed for a long time, but still requires continuous development using approaches to reconstruct the face. The nasolabial sulcus influences facial expression in facial reconstruction. Technological advances improve or facilitate the work of forensic odontologists, anthropologists, and dentists compared to traditional methods.

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