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All Journal Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Mahasiswa Jurusan Teknik Pengairan Jurnal Aisyah : Jurnal Ilmu Kesehatan SCIENCE : Jurnal Inovasi Pendidikan Matematika dan IPA Pharmaceutical Sciences and Research (PSR) Elementary School: Jurnal Pendidikan dan Pembelajaran ke-SD-an JOURNAL OF CHEMICAL NATURAL RESOURCES (JCNaR) Jurnal Karya Abdi Masyarakat Jurnal Ilmiah Ilmu Terapan Universitas Jambi Journal of Pharmaceutical and Sciences.
Utami, Diah Tri
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Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Economics, Econometrics & Finance Education Engineering Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Veterinary

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EFFECTIVENESS OF ANTIOXIDANTS AS BROWNING INHIBITORS AGAINST SHOOT Cyrtostachys renda AS CALLUS CULTURE EXPLANT Syamsurizal, Syamsurizal; Utami, Diah Tri; Elisma, Elisma; Lizawati, Lizawati; Sasongko, Aries Bagus; Prabowo, Beni Hendro
Jurnal Ilmiah Ilmu Terapan Universitas Jambi Vol. 9 No. 1 (2025): Volume 9, Nomor 1, March 2025
Publisher : LPPM Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/jiituj.v9i1.36002

Abstract

Cyrtostachys renda has various pharmacological activities. C. renda roots contain alkaloids and flavonoids as secondary metabolites' main components responsible for anti-inflammatory and anticancer activities. To produce secondary metabolites from C. renda, tissue culture can be used through callus and cell suspension culture techniques. Browning is one of the main challenges in plant tissue culture, which inhibits the success of callus formation, especially in plants with a high content of phenolic compounds such as C. renda. This study tested the effect of soaking in polyvinylpyrrolidone (PVP) and ascorbic acid (AA) in inhibiting the browning process in C. renda explants. Explant colour change testing was done in vitro by soaking the explants in five concentrations of PVP and AA (50, 100, 150, 200 and 250 ppm). Changes in explant colour were then analyzed using the Royal Horticultural Society (RHS) colour chart. The results showed that the PVP antioxidant with a concentration of 200 ppm could suppress explant browning until the fourth day. In comparison, explants treated with AA antioxidants with a concentration of 200 ppm showed browning symptoms on the second day. It can be concluded that antioxidants can reduce the browning level in C. renda shoot explants, and the antioxidant PVP can reduce the browning level better than AA. The mechanism of PVP as an anti-browning agent through inhibition of phenolic oxidation. This study's novelty is obtaining the type of anti-browning PVP with an optimum concentration of 200 ppm to inhibit the browning of C. renda shoot explants.
Accelerated Fermentation of Pineapple Peel-Based Eco-Enzyme with Ganoderma lucidum: A Novel Bioconversion Strategy for Antidermatophytic Maritsa, Hasna Ul; Destiana, Anggari Linda; Husnuddin, Uni Baroroh; Mulyani, Putri Dwi; Utami, Diah Tri; Rasid, Aris Abdul
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 11, No 1 (2026): February 2026
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v11i1.12743

Abstract

Pineapple peel waste, rich in lignocellulosic compounds and bioactive metabolites, remains underutilized despite its potential. Traditional eco-enzyme production from pineapple peels, molasses, and water (3:1:10 ratio) requires 90 days of spontaneous fermentation, limiting efficiency. This study investigates Ganoderma lucidum, a medicinal white-rot fungus, as a biocatalyst to accelerate fermentation and enhance antidermatophytic activity against Trichophyton rubrum. Fermentation substrates were prepared with or without G. lucidum mycelial plugs and monitored at 15 and 45 days for pH, aroma, color, and organoleptic changes. Eco-enzyme efficacy was assessed via agar well diffusion assays at concentrations of 20%, 40%, 60%, 80%, and 100%, measuring T. rubrum colony diameters.Results showed G. lucidum significantly hastened organic decomposition and secondary metabolite production. By day 45, inoculated samples reached pH 2.85 (vs. 3.2 in controls), exhibited faster aroma stabilization and darker coloration, and achieved complete inhibition (0.0 mm colony diameter) across all concentrations. At day 15, inhibition was already strong (0.7 mm vs. 90 mm in untreated controls). This approach reduces fermentation time by over 50%, yielding a potent antifungal agent from waste. It offers a sustainable bioconversion strategy for eco-enzyme production with therapeutic potential.
Co-Authors ., Elisma Anggari Linda Destiana Aries Bagus Sasongko, Aries Bagus Efrini, Emi Elisma Elisma Fitrianingsih Fitrianingsih Halim, Rd Hasnaul Maritsa Husnuddin, Uni Baroroh Isa Ansori Jumardi, Muhammad Ikhsan Lizawati Lizawati Maharini, Indri Oka Lesmana, Oka Prabowo, Beni Hendro Puspa Dwi Pratiwi Putri Dwi Mulyani Rasid, Aris Abdul Sumiadi, Sumiadi Sutrisno, Sutrisno Syamsurizal - - Very Dermawan Yuliawati