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All Journal JURNAL SAINS PERTANIAN EQUATOR Perkebunan dan Lahan Tropika AGRIVITA, Journal of Agricultural Science Indonesian Journal of Pure and Applied Chemistry Jurnal Entomologi Indonesia
Kukuh Hernowo
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Economics, Econometrics & Finance Environmental Science

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Effect of Castanopsis megacarpa Extract on Mortality, Longevity Development and Feeding of Crocidolomia pavonana Larvae Edy Syahputra; Kukuh Hernowo; Riko Riko
AGRIVITA, Journal of Agricultural Science Vol 41, No 3 (2019)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v41i3.2331

Abstract

As several reports of impacts arise due to the use of synthetic insecticides, the investigation for safer alternative insecticides should be continued. Using plant material that has several bioactivities is a promising way out to control insect pest. The objective of this study was to evaluate the effect of leaf and seed extract of Castanopsis megacarpa on mortality, longevity development and feeding preference of Crocidolomia pavonana larvae. Extraction conducted by infusion method. The bioassay assesment was carried out using the residual method. The concentration-mortality relationship was analyzed by probit. The antifeedant test was tested using choice and no-choice methods. Both extracts showed the same strong insecticidal activity, LC50 of 0.18% (leaf) and 0.12% (seed). Leaf and seed extracts at concentrations equivalent to LC50 and LC75 in both methods used showed consistent antifeedant properties. The antifeedant activity of extracts in the no-choice method at both concentrations showed a range of activities of 40.1%-53.7% (leaf) and 74.4%-82.7% (seed) extracts. The leaf and seed extracts of C. megacarpa did not significantly extend the larval development period. Plant material that has more than one bioactivity will be better for suppress the insect pest populations in the field.
ISOLASI ENTOMOPATOGEN LAHAN GAMBUT DI KALIMANTAN BARAT DAN DETERMINASI VIRULENSINYA SEBAGAI MATERIAL BIOINSEKTISIDA Tris Haris Ramadhan; Kukuh Hernowo
Perkebunan dan Lahan Tropika Vol 2, No 2 (2012)
Publisher : Fakultas Pertanian Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (86.347 KB) | DOI: 10.26418/plt.v2i2.3515

Abstract

Entomopatogen merupakan salah satu unsur penting dalam pengendalian hayati dan akan semakin baik jika dikembangkan secara khusus melalui ekplorasi dan pengujian di skala laboratorium. Diharapkan dari penelitian didapatkan entomopatogen lahan gambut yang potensial. Entomopatogen diisolasi dengan cara mengambil cuplikan tanah dan dipanaskan selama 60 menit pada suhu 85 oC. Sedangkan jamur patogen diisolasi dengan teknik mengencerkan cuplikan tanah lalu diinkubasi dalam media agar. Pengujian terhadap serangga di laboratorium dengan metode celup pakan pada media yang mengandung entomopatogen. Kematian serangga akibat perlakuan diamati sampai ulat uji menjadi imago. Hasil pengujian didapatkan tingkat kematian ulat yang diperlakukan dengan bakteri entomopatogen sebesar 33-40 persen sedangkan dengan perlakuan jamur entomopatogen kematian ulat 20-33,3 persen. Kematian pada perlakuan dengan bakteri terjadi pada fase larva-pupa sedangkan perlakuan jamur pada fase larva. Kematian serangga uji terjadi setelah perlakuan 6-18 hari. Isolat bakteri dan jamur entomopatogen dari lahan gambut memberikan harapan untuk dikembangkan sebagai bioinsektisida Kata kunci: Entomopatogen, isolasi, Spodoptera litura
PROFIL GC-MS DAN BIOAKTIVITAS EKSTRAK MINYAK DAUN CENGKEH (Syzygium aromaticum) TERHADAP ULAT GRAYAK Spodoptera frugiperda (GC-MS PROFILING AND BIOACTIVITY OF EXTRACTS CLOVE LEAF OIL (Syzygium aromaticum) AGAINST FALL ARMYWORMS Spodoptera frugiperda) Sitohang, Mikael; Jayuska, Afghani; Hernowo, Kukuh; Alimuddin, Andi Hairil; Aritonang, Anthoni Batahan
Indonesian Journal of Pure and Applied Chemistry Vol 7, No 2 (2024)
Publisher : Tanjungpura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/indonesian.v7i2.77091

Abstract

Clove leaf essential oil can be used as a natural insecticide to control pests. Essential oil obtained by steam distillation produces essential oil with a yield of 1.03%. The essential oil obtained was then analyzed using a GC-MS tool where the aim was to separate the mixture of compounds and identify the compounds contained in clove leaf essential oil. The results of GC-MS analysis of clove leaf essential oil shoved 6 compounds whose similarity was above 84%, where the main compounds were eugenol (74.28%), β-Caryophellene (20.49%), α-Caryophellene (2.32%), Caryophellene oxide (2.26%). The essential oils were then tested for mortality and antifeedant activity against 3rd larva S. frugiperda. Concentrations of 0.1%, 0.3%, 0.5%, 0.7% and 0.9% v/v the essential oil caused 43.44%, 56.66%, 70%, 76.77%, and 86.66% mortality on to tested larva. The probit analysis data the mortality resulted LC50 value of 0.58%. Antifeedant concentrations of 0.37% and 0.58% v/v or equivalent to LC25 and LC50 can reduce 42.41-71.96% of the preferred method and 66.46-86.59% of the non-optioned method.
Aktivitas Nitrat Reduktase Beberapa Morfologi Daun Lada yang Ditanam pada Lahan Gambut Suswanto, Iman; Ramadhan, Tris Haris; Hernowo, Kukuh
Perkebunan dan Lahan Tropika Vol 15, No 1 (2025)
Publisher : Fakultas Pertanian Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/plt.v15i1.93614

Abstract

Leaf organs have an important function as a place for photosynthesis and protein biosynthesis. Photosynthesis ability can be influenced by leaf morphology such as leaf color, leaf area and thickness, number of stomata and leaf shape. Pepper leaves have a variety of shapes that can be used as characteristics of a cultivar. The study aims to obtain information on nitrate reductase activity (NRA) from the leaf morphology of several pepper cultivars planted on peat land. The study was conducted using a non-factorial completely randomized design. The treatments consisted of Bengkayang pepper cultivar leaves divided into three leaf groups (wide, medium and narrow), Indian pepper cultivar leaves and 2 wild peppers in the form of Piper retrofractum and P. colonum. Samples were taken from community plantation, the third youngest leaf of 6 pepper stalks was selected. The results showed that in the Bengkayang pepper population there was a diversity of NRA values. Pepper with the highest ANR was shown in broad-leafed and Indian Bengkayang. The NRA values of broad-leaf, medium-leaf and small-leaf pepper were 5.92; 5.90; 5.81; and 5.78 µmol/hour/gr leaf weight, respectively. In the field, leaf area is an indicator of fast or slow growth rate. The leaf size of wild pepper Piper colonum and P. retrofractum ranges from medium to narrow and has better NRA compared to narrow-leaved Bengkayang
Pertumbuhan dan perkembangan ulat grayak jagung (Spodoptera frugiperda Smith) dengan pakan buatan berbahan dasar kedelai (Glycine max L.): Growth and development of fall armyworm (Spodoptera frugiperda Smith) with soybean-based artificial diet (Glycine max L.) Istiqomah Desi, Isti; Kustiati, Kustiati; Hernowo, Kukuh
Jurnal Entomologi Indonesia Vol 22 No 2 (2025): In Progress
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.22.2.69

Abstract

The fall armyworm (Spodoptera frugiperda Smith) is an invasive pest of corn crops in Indonesia. The development of S. frugiperda rearing techniques based on artificial feed is important to support various research projects on this species. This study aims to study the growth and development of S. frugiperda using soybean-based artificial feed (Glycine max L.). The study utilized first-instar larvae of S. frugiperda adults obtained from the Rasau Jaya Village Corn Farm. A total of 800 larvae were used to obtain accurate and representative data on the insect’s development. The variables observed included several important aspects, such as the survival rate at each stage (from larva to adult), growth morphometry at the sixth instar larva and pupa stages to understand changes in body size and shape, the duration of each stage to determine the length of each developmental phase, and the sex ratio to determine the proportion of males and females in the population. The survival rate data of S. frugiperda imagoes obtained were then analyzed using Microsoft Excel 2013 and presented descriptively to provide a clear and detailed overview of the research results. The research results showed that the survival rate of S. frugiperda at each growth stage averaged above 75%. The lifespan of the larval stage was 12.80 ± 1.00 days, the pupal stage was 7.80 ± 0.77 days, and the female imago (9.55 ± 2.42 days), while the male (7.39 ± 1.77 days). The sex ratio of males to females is 1:1.02. These results indicate the excellent potential of soybean-based artificial feed as the primary diet for maintaining S. frugiperda in the laboratory.
PENGARUH PUPUK NPK TERHADAP HASIL UBI JALAR AKSESI ARF-14 PADA BEBERAPA UMUR PANEN DI TANAH PMK Maulana, Shiva; Hernowo, Kukuh; Arifin, Nur
Jurnal Sains Pertanian Equator Vol 15, No 1: In Press
Publisher : Fakultas Pertanian Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/jspe.v15i1.95369

Abstract

Ubi jalar (Ipomoea batatas L.) merupakan jenis umbi-umbian yang memiliki banyak keunggulan dibandingkan dengan jenis umbi lainnya, yaitu kandungan karbohidrat dan energi yang tinggi. Ubi jalar dapat tumbuh pada beberapa jenis tanah satu diantaranya tanah PMK. Penelitian ini bertujuan untuk mengkaji pengaruh pemberian pupuk NPK dan umur panen terhadap kualitas hasil ubi jalar Aksesi ARF-14 pada tanah PMK. Penelitian ini menggunakan metode Rancangan Acak Lengkap (RAL) yang disusun dengan Rancangan Petak Terbagi (RPT). Masing-masing perlakuan terdiri dari 3 ulangan, sehingga terdapat 27 unit percobaan. Petak utama adalah umur panen tanaman ubi jalar Aksesi ARF-14 (b), yaitu: b1 = 90 HST, b2 = 105 HST, b3 = 120 HST. Anak petak dalam penelitian ini adalah dosis pupuk NPK (p), yaitu: p1 = tanpa pupuk NPK, p2 = 150 kg/ha, p3 = 300 kg/ha. Variabel yang diamati dalam penelitian ini yaitu jumlah umbi per tanaman, berat umbi per tanaman, ukuran umbi, indikator serangan hama penggerek, kadar gula brix, dan kadar serat. Berdasarkan hasil penelitian dapat disimpulkan bahwa hasil ubi jalar Aksesi ARF-14 yang terbaik yaitu kombinasi perlakuan umur panen 120 HST dan dosis pupuk NPK 300 kg/ha.
PENGARUH BOKASHI AMPAS TEBU DAN KALIUM TERHADAP PERTUMBUHAN DAN HASIL LOBAK PUTIH PADA TANAH ALUVIAL Dewi, Jeananda Kusuma; Palupi, Tantri; Hernowo, Kukuh
Jurnal Sains Pertanian Equator Vol 15, No 1: In Press
Publisher : Fakultas Pertanian Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/jspe.v15i1.99185

Abstract

Lobak (Raphanus sativus L.)merupakan jenis tanaman sayuran semusim yang berbentuk umbi dan dapat tumbuh di berbagai dataran yang beriklim tropis. Lobak memiliki banyak kandungan vitamin, mineral dan kandungan gizi lainnya seperti fosfor, kalsium, vitamin A, B1 dan C, sehingga lobak dibudidayakan sebagai tanaman sayur dan obat. Penelitian ini bertujuan untuk mengetahui pengaruh bokashi ampas tebu dan kalium terhadap pertumbuhan dan hasil lobak putih pada tanah aluvial serta mendapatkan dosis bokashi ampas tebu dan kalium yang terbaik untuk pertumbuhan dan hasil lobak putih pada tanah aluvial. Penelitian dilaksanakan di halaman Asrama Bengkayang, Jalan Sepakat 2, Kecamatan Pontianak Tenggara, Kalimantan Barat. Penelitian berlangsung selama 3 bulan. Penelitian ini menggunakan polybag dengan metode Rancangan Acak Lengkap Faktorial yang terdiri dari 2 faktor perlakuan. Faktor pertama yaitu bokashi ampas tebu (B) yang terdiri dari 3 taraf yaitu 10 ton/ha, 20 ton/ha, 30 ton/ha. Faktor kedua yaitu pupuk Kalium (K) yang terdiri dari 3 taraf yaitu 150 kg/ha, 300 kg/ha, 450 kg/ha, sehingga didapat 9 kombinasi perlakuan dan setiap perlakuan diulang sebanyak 3 kali. Masing-masing kombinasi perlakuan terdapat 3 tanaman sampel, sehingga terdapat 81 sampel tanaman. Variabel yang diamati meliputi: jumlah daun, berat kering tanaman, berat segar umbi, panjang umbi dan diameter umbi. Hasil penelitian menunjukkan bahwa bahwa dosis bokashi ampas tebu dan kalium yang diuji pada penelitian ini belum memberikan pengaruh yang signifikan terhadap pertumbuhan dan hasil lobak putih pada tanah aluvial, serta berat segar yang dihasilkan pada penelitian ini masih terbilang rendah jika dibandingkan dengan deskripsi tanaman lobak, sehingga perlu dilakukan peningkatan hasil melalui perbaikan faktor-faktor penting.
Co-Authors Andi Hairil Alimuddin Aritonang, Anthoni Batahan Dewi, Jeananda Kusuma Edy Syahputra Iman Suswanto Istiqomah Desi, Isti Jayuska, Afghani Kustiati Kustiati Maulana, Shiva Nur Arifin, Nur Riko Riko Sitohang, Mikael Tantri Palupi TRIS HARIS RAMADHAN