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All Journal Indonesian Journal of Pharmaceutical Science and Technology Science and Technology Indonesia Padjadjaran Journal of Dentistry Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) AKSIOLOGIYA : Jurnal Pengabdian Kepada Masyarakat Farmasains : Jurnal Ilmiah Ilmu Kefarmasian Jurnal Pengabdian Kepada Masyarakat MEMBANGUN NEGERI JOURNAL OF COMMUNITY ENGAGEMENT AND EMPOWERMENT
Kurniawan, Muhammad Fariez
Universitas Muhammadiyah Yogyakarta
Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Dentistry Environmental Science Health Professions Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Medicine & Pharmacology Nursing Physics Public Health Social Sciences Veterinary Other

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Analysis of Hydrolyzed Collagen in Facial Serum for Halal Authentication Using FTIR Spectroscopy and Multivariate Calibration Salamah, Nina; Firdaus, Rizqi Amalia; Guntarti, Any; Ahda, Mustofa; Uddin, Abm Helal; Kurniawan, Muhammad Fariez
Science and Technology Indonesia Vol. 10 No. 4 (2025): October
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2025.10.4.1270-1279

Abstract

Cosmetic trends in Indonesia are on the rise, particularly for facial serum products. However, most of these products lack halal certification, posing an important concern for Muslim consumers due to the potential use of haram substances, such as pork gelatin. This study seeks to identify the source of gelatin in serum products using the Fourier Transform Infrared (FTIR) spectroscopy and chemometric analysis. The samples used in this study include a reference facial serum formulated with pure bovine and porcine gelatin concentrations in the ratios of 1:0, 8:2, 5:5, 2:8, and 0:1, three hydrolyzed collagen serums that are not yet labeled halal and available on Shopee, and one serum that is labeled halal. The method used was FTIR combined with PLS chemometrics and PCA. The isolation of facial serum gelatin was performed using acetone at -20oC, followed by analysis via FTIR at wave numbers ranging from 4000 to 400 cm-1. FTIR results indicated the presence of functional groups in gelatin constituents, including C O, N H, C N, and C H aliphatic. The wavelengths employed for PLS and PCA analysis ranged from 1631 to 1430 cm-1. Calibration results showed R2=0.9936 and RMSEC=3.0445%. Internal validation yielded RMSECV=0.1674% and R2=0.9994 whilst external validation yielded RMSEP=0.9981% and R2=0.9910. Lastly, PCA analysis revealed that one halal-labeled serum sample contained bovine gelatin, whereas the three examined commercial serums were free from both pork and bovine gelatin.
Co-Authors Adienia, Vitrisia Aji Winanta Andewa, Shylvia Muchsin Andrian, Zulkifli Anggraini, Dita Annisa, Amartya Nadhia Any Guntarti Baeha, Wahdatul Khairat Dewi Melani Hariyadi Dwi Setyawan Fajarani, Rizky Anita Firdaus, Moch Arriyan Firdaus, Rizqi Amalia Iskak, Rifki Umami Isnan, Ahmad Arif Kemal Asyidiq, Muhammad Maziyah, Nurul Mustofa Ahda Nina Salamah Nining Sugihartini Nisa, Alditta Khoirun Pinasti Utami, Pinasti Pratama, Zikri Fatua Rizki Amalia, Adhitya Anisa Rizqi, Additya Sabeta, Meilidya Vani Septiawan, Darul Supriyono, Devany Vinandhita Tedjo Yuwono Tita Ratya Utari Uddin, ABM Helal Vella Lailli Damarwati Wardany, Herdita Nugraheny Kusuma WIDYASTUTI Zahira, Safna Tiara