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All Journal Biotika: Jurnal Ilmiah Biologi Indonesian Journal of Biotechnology Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat BERITA BIOLOGI JC-T (Journal Cis-Trans): Jurnal Kimia dan Terapannya Jurnal Kimia Terapan Indonesia Chemistry Indonesian Journal of Chemical Research Martabe : Jurnal Pengabdian Kepada Masyarakat International Journal of Community Service Learning Walisongo Journal of Chemistry Molekul: Jurnal Ilmiah Kimia Journal of Educational Chemistry (JEC) Prosiding Konferensi Nasional PKM-CSR Molekul: Jurnal Ilmiah Kimia
Tahya, Candra Yulius
Fakultas Ilmu Pendidikan, Program Studi Pendidikan Kimia, Universitas Pelita Harapan, Tangerang 15811, Banten, Indonesia.
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Control & Systems Engineering Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Nursing Public Health Social Sciences

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Journal : BERITA BIOLOGI

 1 
ISOLASI DAN KARAKTERISASI ISOLAT BAKTERI RESISTEN TEMBAGA DARI SUNGAI CISADANE Wahyu Irawati; Candra Yulius Tahya
BERITA BIOLOGI Vol 19, No 3B (2020)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v19i3B.3937

Abstract

The water quality of the Cisadane River, located in the industrial factory area, it is contaminated with copper, it is necessary to treat sewage. Biological wastewater treatment using bacteria is a more economical and effective method of removing copper from the environment than chemical methods. This study aims to isolate, identify, determine the level of resistance, and analyze the ability of bacteria to accumulate copper. Isolation was carried out by the spread method in Luria Bertani Agar medium containing CuSO4. Bacterial isolates were identified based on analysis of the 16S rDNA gene. The resistance level is determined based on the Minimum Inhibitory Concentration (MIC) value. The accumulation ability of copper was determined using an atomic absorption spectrophotometer. Six bacteria resistant to copper were isolated and identified as Pantoea agglomerans strain IrCis1, Klebsiella grimontii strain IrCis3, Klebsiella penumoniae strain IrCis4, Shigella flexneri strain IrCis5, Enterobacter cloacae strain IrCis6, and Enterobacter cloacae strain IrCis9 with MIC of 7 mM - 9 mM. Pantoea agglomerans strains IrCis1, Klebsiella grimontii strains IrCis3, Klebsiella pneumoniae strains IrCis4 can accumulate copper of 1,19 mg, 1,39 mg, and 0,92 mg, respectively, per gram of dry cell weight.  
Co-Authors Adila, Mahda Uli Benedict Iglecias Sihombing Debora Sitinjak Elen Tiwery Elfani Bermuli, Jessica Elisabeth Wulan Kainde Friska J. Purba Friska Purba Gaspersz, Nelson Girsang, Thereshia Margaretta Greisnaningsi Greisnaningsi Hardy, Junius Iglecias Sihombing, Benedict Inabuy, Marchella Kainama, Healthy Karnelasatri Karnelasatri Karnelasatri, . Kelly Sinaga Kelly Sinaga Lase, Joy Sisokhi Putra Mega Gracelia Monaten Melanie Cornelia Melanie Cornelia, Melanie Muhammad Ikhsan Taipabu Munthe, Sri Wahyu Ningsih Napitupulu, Happy Chelsy Betaria Pangela, Misael Ivan Purba, Elisabeth Purba, Friska Purba, Friska Juliana Ringo, Sisilya Siringo Sihombing, Chatrina Yohana Simamora, Adela Rosalia sinaga, kelly Sitinjak, Debora Suryani Tagor M Siregar Tagor M. Siregar Tahya, Kartika Telaumbanua, Hepy Terima Tryani K. Junita Lumbantoruan Wahyu Irawati WAHYU IRAWATI