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All Journal HAYATI Journal of Biosciences Biotika: Jurnal Ilmiah Biologi AL KAUNIYAH Jurnal Sain Peternakan Indonesia Dharmakarya : Jurnal Aplikasi Ipteks Untuk Masyarakat Jurnal Profesi Medika: Jurnal Kedokteran dan Kesehatan Surya Octagon Interdisciplinary Journal of Science and Technology Floribunda ASEAN Journal of Community Engagement Current Research on Biosciences and Biotechnology Makara Journal of Science
Dwiranti, Astari
Departemen Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Indonesia, Depok, 16424, Indonesia, (021) 7270163.
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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Journal : Current Research on Biosciences and Biotechnology

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Assessment of potential cellular toxicity of methylglyoxal on primary human epidermal keratinocytes Prakoso, Nurul Muhammad; Sundari, Ayu Mulia; Fadhilah; Abinawanto; Dwiranti, Astari; Bowolaksono, Anom
Current Research on Biosciences and Biotechnology Vol. 6 No. 2 (2025)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2025.6.2/JYICDMXO

Abstract

Skin is the outermost layer of the human body, which is important to prevent damage by infection, ultraviolet radiation, and toxic materials. The impermeable barrier of the skin has to be maintained via skin regeneration to preserve its essential function. However, skin regeneration declines due to decreased cell proliferation during skin aging. In this study, we investigated the role of methylglyoxal (MGO) as a precursor of advanced glycation end-products (AGE), which have been reported as an emerging threat of early aging in the skin. Previous studies reported a reduction in survival of the HaCaT cell line exposed to 500 µM MGO. However, studies on primary keratinocytes are still limited. We evaluated the viability and proliferation of primary human epidermal keratinocytes (HEKa) under the exposure of MGO. We also employed H2DCFDA assay to quantify the generation of intracellular ROS due to MGO. Our study revealed a biphasic response in which lower doses of MGO (25 and 50 µM) increased viability, while the cellular toxicity began at higher concentrations of 100, 200, and 300 µM. The proliferation assay also suggests an overall reduction of primary epidermal keratinocytes exposed to 100 and 200 µM MGO after 24 and 48 hours of culture. Increasing ROS concentrations were also observed in cells treated with 100 and 200 µM MGO. Our data suggested that a lower concentration of MGO was required to induce cellular toxicity in primary human epidermal keratinocytes compared to the HaCaT cell line reported by another study, indicating different sensitivity to MGO.
Caffeine as a modulator of redox balance and migration in MDA-MB-231 triple-negative breast cancer cells Amira, Qanita Hana; Fadilah; Bowolaksono, Anom; Dwiranti, Astari
Current Research on Biosciences and Biotechnology Vol. 6 No. 2 (2025)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2025.6.2/54S7U9DO

Abstract

Caffeine, a widely consumed stimulant, has demonstrated significant effects on cancer cell behavior, particularly in triple-negative breast cancer (TNBC) cells. This study investigates the impact of caffeine on MDA-MB-231 cells, focusing on cell morphology, viability, antioxidant gene expression, and cell migration. Caffeine at concentrations of 10 mM and above induced notable morphological changes, including cell rounding, detachment, and decreased cell density, indicative of cytotoxic effects and cellular stress responses such as apoptosis. Viability assays revealed a dose-dependent reduction in cell survival, with a substantial decline in total cell count at higher caffeine concentrations. The observed decrease in cell viability is associated with the downregulation of antioxidant genes SOD2 and GLO1, suggesting disrupted redox balance and impaired detoxification systems. A significant positive correlation was noted between SOD2 and GLO1 expression levels, indicating their interdependence in antioxidant defense mechanisms. Additionally, caffeine treatment impaired cell migration, as the wound healing assay shows, with higher concentrations significantly hindering wound closure. This effect on migration, relevant for metastasis, aligns with findings from previous studies on caffeine’s influence on cancer cell motility. The results suggest that 10 mM caffeine may serve as an optimal concentration for inducing cellular stress without immediate, widespread cell death, positioning it as a promising candidate for further therapeutic exploration.
Co-Authors Abinawanto Abinawanto Abinawanto Adra Amalia Nur Ahlina Amira, Qanita Hana Andi Salamah Anna Widyastuti ANOM BOWOLAKSONO Arief Boediono Atria, Mega Deti Purwanti Fadhilah Fadilah Funahashi, Hiroaki Iriani, Nur Annisa Isnaenisa Rachma Khayrani, Apriliana Cahya Kitty, Yelina Lischer, Kenny Mouleidi Dwi Putri Nadhifa Tazkia Ramadhani Niarsi Merry Hemelda Niarsi Merry Hemelda Niarsi Merry Hemelda Nidaan Khafian Pambudi, Sabar Prakoso, Nurul Muhammad Putrika, Afiatry Rajagukguk, Selly Setiati Ratna Yuniati SAHLAN, MUHAMAD Sarah Tsabituddinillah Sari, Elvita Shela Kartika Wijaya Siska Apriliani Sri Rahayu, Amy Yayuk Sundari, Ayu Mulia Utomo, Doddy Irawan Setyo Windri Handayani Windri Handayani Windri Handayani Wulandari, Tri Aprilliana