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All Journal Jurnal Sains Teknologi dan Lingkungan (JSTL) Jurnal Biologi Tropis Jurnal Pengabdian Magister Pendidikan IPA JITPI : Jurnal Ilmu Dan Teknologi Peternakan Indonesia (Indonesian Journal of Animal Science and Technology) Alamtana
Rozi, Tapaul
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Immunology & microbiology Public Health

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Genetic Diversity of Bali Cattle Base on Two Microsatellite Loci - INRA032 and BM2113 Maskur, Maskur; Rozi, Tapaul; Rahmajan, Rahmajan; Kasip, Lalu; Muhsinin, M.
Jurnal Biologi Tropis Vol. 24 No. 1b (2024): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i1b.8076

Abstract

Microsatellites are short tandem repeat (STR) sequences that consist of simple repeats and exhibit a high number of alleles at each genomic locus. The aim of this study was to examine genetic variation in Bali cattle and the population dynamics using microsatellite markers. Two microsatellite loci, INRA032 and BM2113 were amplified using PCR Total DNA samples from the genome of 60 Bali cattle, then The PCR products were analyzed through agarose gel electrophoresis, followed by staining with Ethidium Bromide (EtBr). The number and size of alleles that appeared on the gel, while the diversity and population dynamics were analyzed using Popgene version 1.31 and Gene Calc. In this study, The effective number of alleles for the two microsatellite loci in the Bali cattle population analyzed was 6.53, with an average of 3.27. The average PIC of the two microsatellite loci was 0.70, while the observed heterozygosity (Ho) exceeded the expected heterozygosity (He), with values of 0.975 and 0.695, respectively. This suggests an excess of heterozygosity in the population and indicates that the populations are not in Hardy-Weinberg equilibrium. The inbreeding coefficient was high, with negative Fis values of -0.38 for INRA032 and -0.43 for BM2113.
Association of Inducible Nitric Oxide Synthase (iNOS) Gene Diversity with Immunity Characteristics in Kampung Chicken Muhsinin, Muhammad; Jan, Rahma; Maskur, Maskur; Rozi, Tapaul; Kasip, Lalu Muhammad; Al Farizi, Muhammad Salman
Jurnal Biologi Tropis Vol. 25 No. 1 (2025): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i1.8296

Abstract

The immune system of Kampung chickens is vital for maintaining health and productivity, especially in combating bacterial infections. Among the genetic components involved, the inducible nitric oxide synthase (iNOS) gene is crucial for producing nitric oxide (NO), a molecule with strong antimicrobial properties. This study investigated the association between iNOS (AluI│g.15056T>C) gene polymorphism and immune traits in Kampung chickens to enhance disease resistance. Blood samples from 100 Kampung chickens were genotyped using PCR-RFLP, and immune parameters such as leukocyte count, macrophage activity, and bacterial resistance were evaluated. Statistical analysis revealed significant differences among TT, TC, and CC genotypes. The CC genotype exhibited superior performance, including the highest macrophage activity (91.74±1.92%), activated macrophage capacity (2279.49), and bacterial death rate (60.81±3.54%). These findings suggest that the CC genotype enhances NO production, strengthening the immune response to bacterial pathogens like Staphylococcus aureus and Salmonella Pullorum. Additionally, the polymorphism contributes to improved genetic diversity and immune efficiency in Kampung chickens. The study highlights the potential of incorporating the iNOS CC genotype in selective breeding programs to produce chickens with enhanced resilience. Future research should focus on interacting environmental factors with iNOS expression to optimize its application in chicken production.
Identification of Diversity and Genetic Distance of Indonesian Local Chicken Strains Based on Myostatin Gene Muhsinin, Muhammad; Maskur, Maskur; Jan, Rahma; Rozi, Tapaul; Kasip, Lalu Muhammad; Al Farizi, Muhammad Salman
Jurnal Biologi Tropis Vol. 25 No. 1 (2025): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i1.8297

Abstract

Indonesian local chickens are essential genetic resources with significant potential for advancing the national poultry industry due to their high genetic diversity, reflecting adaptation to tropical environments and traditional farming systems. This study analyzed the genetic diversity of Kampung, Sentul, KUB, SenSi, and Broiler chickens based on the MSTN g.4842T>G locus. Blood samples from 150 chickens underwent PCR-RFLP analysis using the BsrI restriction enzyme targeting exon 2 of the MSTN gene. Parameters such as observed heterozygosity (Ho), expected heterozygosity (He), polymorphism information content (PIC), and Hardy-Weinberg equilibrium were assessed. Genetic distances were calculated using PHYLIP, and a dendrogram was constructed with UPGMA. The study identified two alleles (G and T) and three genotypes (GG, GT, TT), with the T allele being dominant, particularly in Broiler chickens (78.3%). Ho was lower than He in all populations, indicating a heterozygosity deficit. PIC values ranged from 0.282 (Broiler) to 0.367 (SenSi), classifying the MSTN locus as moderately polymorphic. Kampung and KUB chickens showed the closest genetic relationship, while Broiler chickens were the most distinct. These findings underscore the potential of local chickens in breeding programs for adaptability and productivity. Further research with additional markers is recommended to enhance sustainable poultry farming.
Identification of Single Nucleotide Polymorphism (SNP) in Exon 5 of the Prolactin Gene in Native Chickens Rozi, Tapaul; Maskur, Maskur; Jan, Rahma; Kasip, Lalu Muhammad; Muhsinin, Muhammad
Jurnal Biologi Tropis Vol. 25 No. 1 (2025): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i1.8342

Abstract

The prolactin gene (PRL) in chickens is one of the genes that regulates broodiness and egg production in laying hens. Single Nucleotide Polymorphism (SNP) is a form of single nucleotide variation in the DNA base sequence at specific locations in the genome that can influence differences in quantitative traits in livestock. This study aimed to identify SNPs in the prolactin gene of native chickens. The research methods included DNA extraction, PCR amplification, and DNA sequence analysis for genotype determination of individuals. The results revealed four mutation points at positions g.8052 bp T>C, g.7886 T>A, g.7823 A>G, and g.8069 T>C. All mutation points in exon 5 were substitution mutations. Statistical analysis showed that all loci were polymorphic and the population was in Hardy–Weinberg equilibrium.
Co-Authors Ahmad Jupri Al Farizi, Muhammad Salman Atsiqah, Fadhlin Akbar Budi Indarsih, Budi Damayanti , Eva Fadli Fadli Hilman Ahyadi Husain, Pahmi Isrowat Isrowati Jannatun, Baiq Haulia Jaya, I Nyoman Sukartha Jupri , Ahmad Kasip, L. Kasip, Lalu Kuncoro, Zain Bagus Lalu Muhammad Kasip Lilik Hidayati, Lilik Maelia, Wina Maskur Maskur Maulida, Khoviva Muhsinin, M. Muhsinin, Muhammad Ni Ketut Dewi Haryani, Ni Ketut Dewi NURUL HIDAYAH Oka, Risky Agustina P., Eka Sunarwidi Rahma Jan Rahmajan, Rahmajan Septian, I Gede Nano sydqy, Shohibul Tamzil, Moh Hasil Umrah Umrah