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Penetapan Kadar Kurkuminoid dalam Ekstrak Campuran Curcuma domestika Val dan Curcuma xanthorrizha Roxb. Sebagai Bahan Baku Jamu Saintifik Secara KLT - Densitometri risthanti, reine risa; sumiyani, ririn; wulansari, devyani diah; anawati, tita juli
Pharmaceutical Journal of Indonesia Vol 5, No 1 (2019)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Terapi alternatif menggunakan bahan alam semakin diminati oleh masyarakat belakangan ini. Salah satu terapi alternative yang digemari adalah jamu. Penggunaan jamu sebagai terapi alternative perlu dibuktikan secara ilmiah melalui proses saintifikasi jamu. Jamu saintifik merupakan terobosan yang diharapkan dapat digunakan dalam pelayanan kesehatan sebagai terapi alternatif. Kontrol kualitas pada jamu perlu dilakukan untuk menjamin efektivitas dan keamanannya. Pada penelitian ini akan dilakukan kontrol kualitas pada bahan baku formula jamu antihiperurisemia yaitu ekstrak campuran kunyit (Curcuma domestica Val.) dan temulawak (Curcuma xanthorhiza Roxb.). Tujuan penelitian ini untuk mentapkan kadar kurkuminoid dalam ekstrak campuran kunyit dan temulawak sebagai bahan baku jamu saintifik antihiperurisemia secara KLTdensitometri. Ekstrak campuran kunyit dan temulawak diperoleh melalui proses ekstraksi dengan metode Ultrasonic-assisted extraction (UAE) dengan pelarut etanol p.a. Fase diam yang digunakan adalah silika gel 60 F254 nm dan fase gerak yang digunakan adalah kloroform-metanol (9:1). Berdasarkan hasil validasi metode, kurkumin dapat terpisah dengan baik dari kurkuminoid dengan nilai Rs = 2 dan koefisien korelasi kurva baku pada rentang kadar 10-800 ppm adalah r = 0,9803. Metode ini juga menghasilkan presisi dan akurasi yang baik yaitu RSD = 4,55% untuk presisi dan 89,00% - 97,35% untuk akurasi, sesuai dengan yang dipersyaratkan oleh AOAC. Sensitivitas yang dihasilkan adalah 4,31 ppm untuk LOD dan 14,37 ppm untuk LOQ. Hasil penetapan kadar kurkuminoid diperoleh kadar kurkuminoid rata-rata pada sampel 1a, 1b, dan 1c berturut-turut adalah 9,83%, 9,92% dan 10,72%.

Penetapan Kadar Kurkuminoid dalam Ekstrak Campuran Curcuma domestika Val dan Curcuma xanthorrizha Roxb. Sebagai Bahan Baku Jamu Saintifik Secara KLT - Densitometri risthanti, reine risa; sumiyani, ririn; wulansari, devyani diah; anawati, tita juli
Pharmaceutical Journal of Indonesia Vol 5, No 1 (2019)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Terapi alternatif menggunakan bahan alam semakin diminati oleh masyarakat belakangan ini. Salah satu terapi alternative yang digemari adalah jamu. Penggunaan jamu sebagai terapi alternative perlu dibuktikan secara ilmiah melalui proses saintifikasi jamu. Jamu saintifik merupakan terobosan yang diharapkan dapat digunakan dalam pelayanan kesehatan sebagai terapi alternatif. Kontrol kualitas pada jamu perlu dilakukan untuk menjamin efektivitas dan keamanannya. Pada penelitian ini akan dilakukan kontrol kualitas pada bahan baku formula jamu antihiperurisemia yaitu ekstrak campuran kunyit (Curcuma domestica Val.) dan temulawak (Curcuma xanthorhiza Roxb.). Tujuan penelitian ini untuk mentapkan kadar kurkuminoid dalam ekstrak campuran kunyit dan temulawak sebagai bahan baku jamu saintifik antihiperurisemia secara KLTdensitometri. Ekstrak campuran kunyit dan temulawak diperoleh melalui proses ekstraksi dengan metode Ultrasonic-assisted extraction (UAE) dengan pelarut etanol p.a. Fase diam yang digunakan adalah silika gel 60 F254 nm dan fase gerak yang digunakan adalah kloroform-metanol (9:1). Berdasarkan hasil validasi metode, kurkumin dapat terpisah dengan baik dari kurkuminoid dengan nilai Rs = 2 dan koefisien korelasi kurva baku pada rentang kadar 10-800 ppm adalah r = 0,9803. Metode ini juga menghasilkan presisi dan akurasi yang baik yaitu RSD = 4,55% untuk presisi dan 89,00% - 97,35% untuk akurasi, sesuai dengan yang dipersyaratkan oleh AOAC. Sensitivitas yang dihasilkan adalah 4,31 ppm untuk LOD dan 14,37 ppm untuk LOQ. Hasil penetapan kadar kurkuminoid diperoleh kadar kurkuminoid rata-rata pada sampel 1a, 1b, dan 1c berturut-turut adalah 9,83%, 9,92% dan 10,72%.

Ameliorative Effect of Carica papaya Seed Extract on Diabetic Rat Model with Muscle Atrophy Devyani Diah Wulansari; Devyana Dyah Wulandari; Reine Risa Risthanti; Aguslina Kirtishanti
MPI (Media Pharmaceutica Indonesiana) Vol. 2 No. 4 (2019): DECEMBER
Publisher : Fakultas Farmasi, Universitas Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24123/mpi.v2i4.2184

This study aims to prove that papaya seed extract has the potential to improve muscle cell atrophy in diabetic conditions. Type 2 diabetes mellitus (DM) animal model were made with conditions similar to type 2 DM in humans i.e. with long-term (56 days) high sugar (fructose) consumption. Then, the next 14 days were treated with papaya seed extract at the dose of 100, 200, and 300 mg/kg BW orally. Body weight and blood glucose levels were monitored throughout the study period. At the end of the study, histopathological examination of gastrocnemius skeletal muscle tissue was carried out using hematoxylin-eosin staining and measuring myocyte cell area. Results showed that there is a correlation between blood glucose levels with the area of muscle cells which explains that the decrease in blood glucose levels is in line with the increase in the area of muscle fiber cells. In the diabetic group and the treatment group the dose of 100 mg/kg BW has a smaller area, whereas in the treatment group the dose of 200 and 300 mg/kg BW has an area close to the area of the muscle cell in the normal group. This is supported by the results of measurements of the area of muscle fiber cells observed through cross sections and measured at the end of this study. The treatment group at the dose of 200 and 300 mg/kg BW did not have significant difference (P>0.05) compared to the normal group. It can be concluded that papaya seed extract therapy at the dose of 200 and 300 mg/kg BW on diabetic rats can reduce the fasting blood glucose levels so that it can ameliorate cell atrophy in the diabetic conditions.

Empowering Future Pharmacists in Chemistry Analysis of Nutraceuticals Setiawan, Vendra; Risthanti, Reine Risa; Makayasa, Citra Hayu; Alkindi, Fawandi Fuad; Cahyani, Ayu Eprilita Fitri Ika; Makhnunah, Ninis
Amalee: Indonesian Journal of Community Research and Engagement Vol 5 No 2 (2024): Amalee: Indonesian Journal of Community Research and Engagement
Publisher : LP2M INSURI Ponorogo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37680/amalee.v5i2.5258

The community service aims to empower future pharmacists in East Java, Indonesia, by enhancing their understanding of nutraceuticals through chemistry analysis. Students gain essential skills for their academic pursuits through practical training in chromatography analysis and antioxidant activity measurements. The service equips students with practical skills in analyzing nutraceuticals, preparing them for further pharmacy studies. A training program was carried out for 88 SMA and SMK Farmasi students using Participatory Action Research (PAR). The main subjects covered were analysis using thin-layer chromatography, evaluation of functional groups in polyphenols, and assessment of antioxidant effectiveness. Pharmacy lecturers facilitated the program, guiding participants in practical competencies. The outcomes were successful, with a better understanding of nutraceuticals and valuable analytical skills gained. The significance of practical training and educational interventions in preparing students for advanced pharmacy studies is emphasized by this service.

NT-proBNP LEVEL CHANGES AFTER COMBINATION THERAPY WITH BISOPROLOL AND ACE-INHIBITOR IN PATIENT WITH HEART FAILURE Risthanti, Reine Risa; Aminuddin, Muhammad; Suharjono, Suharjono
Folia Medica Indonesiana Vol. 52 No. 4 (2016): OCTOBER - DECEMBER 2016
Publisher : Universitas Airlangga

Heart failure (HF) is the final common stage of many diseases of the heart. NT-proBNP levels are increased in HF and correlate well with ventricular wall stress and severity of HF. Combination therapy with Bisoprolol and ACE-inhibitor decreases NT-proBNP level in patient with HF. The use of Bisoprolol as a combination with ACE-inhibitor is still dominate in outpatient setting at Dr. Soetomo teaching hospital. The objective of this study is to analyze NT-proBNP level changes as an indicator in cardiac function after combination therapy with Bisoprolol and ACE-inhibitor in patient with HF.Methods: This study was prospective, observational and conducted in outpatient setting. Consecutive patients who meet the inclusion criteria of the study were included. Blood samples were taken at pre and 2 months post combination therapy with Bisoprolol and ACE-inhibitor, then NT-proBNP level was measured with IMMULITE®. There were 14 patients enrolled in this study (7 males, 7 females). The result showed that NT-proBNP 2 months post combination therapy with Bisoprolol and ACE-inhibitor is significantly decreased than baseline with mean baseline of NT-proBNP level is 4191.43 ± 4367.277 pg/ml to 2786.79 ± 2485.199 pg/ml (p=0.025). From a total 14 patients, 9 patients had NT-proBNP decreases >20% (20.1% – 56.4%) and 3 patients had NT-proBNP decreases <20% (1.8%, 6.6%, and 12.4%). There were 2 patients with NT-proBNP increases >40% (43.4% and 40.4%). In conclusion, there was a significant decreases in NT-proBNP level after 2 months combination therapy with Bisoprolol and ACE-inhibitor in patient with HF.

Empowering Future Pharmacists in Chemistry Analysis of Nutraceuticals Setiawan, Vendra; Risthanti, Reine Risa; Makayasa, Citra Hayu; Alkindi, Fawandi Fuad; Cahyani, Ayu Eprilita Fitri Ika; Makhnunah, Ninis
Amalee: Indonesian Journal of Community Research and Engagement Vol. 5 No. 2 (2024): Amalee: Indonesian Journal of Community Research and Engagement
Publisher : LP2M INSURI Ponorogo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37680/amalee.v5i2.5258

The community service aims to empower future pharmacists in East Java, Indonesia, by enhancing their understanding of nutraceuticals through chemistry analysis. Students gain essential skills for their academic pursuits through practical training in chromatography analysis and antioxidant activity measurements. The service equips students with practical skills in analyzing nutraceuticals, preparing them for further pharmacy studies. A training program was carried out for 88 SMA and SMK Farmasi students using Participatory Action Research (PAR). The main subjects covered were analysis using thin-layer chromatography, evaluation of functional groups in polyphenols, and assessment of antioxidant effectiveness. Pharmacy lecturers facilitated the program, guiding participants in practical competencies. The outcomes were successful, with a better understanding of nutraceuticals and valuable analytical skills gained. The significance of practical training and educational interventions in preparing students for advanced pharmacy studies is emphasized by this service.

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