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All Journal ACTA VETERINARIA INDONESIANA Jurnal Sains Peternakan Jurnal Biologi Tropis Martabe : Jurnal Pengabdian Kepada Masyarakat Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Sciences) Jurnal Ilmiah Fillia Cendekia JURNAL KAJIAN VETERINER Bioscientist : Jurnal Ilmiah Biologi BIOGENIC: Jurnal Ilmiah Biologi
Rahmaniar, Reina Puspita
Universitas Wijaya Kusuma Surabaya
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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PENCEGAHAN MASTITIS SUBKLINIS PADA PETERNAKAN SAPI PERAH DI BENDUL MERISI SURABAYA Reina Puspita Rahmaniar; Dyah Widhowati; Hana Cipka Pramuda Wardhani
Martabe : Jurnal Pengabdian Kepada Masyarakat Vol 6, No 7 (2023): Martabe : Jurnal Pengabdian Kepada Masyarakat
Publisher : Universitas Muhammadiyah Tapanuli Selatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31604/jpm.v6i7.2621-2627

Abstract

Tujuan dari kegiatan pengabdian Masyarakat ini untuk meningkatkan kualitas susu melalui pencegahan mastitis subklinis pada wilayah peternakan sapi perah di Bendul Merisi Surabaya. Urgensi dilakukan pengabdian ini yaitu atas dasar tidak adanya KUD yang mengontrol Kesehatan sapi perah di wilayah tersebut, penyakit mastitis subklinis mempengaruhi kualitas susu namun tidak dapat dilihat secara langsung oleh peternak, lokasi peternakan dekat dengan penduduk, sehingga perlu edukasi agar tidak mudah terjadi kontaminasi silang dari agen infeksi yang menyebabkan penyakit. Pelaksanaan pengabdian ini terdiri beberapa tahapan. Pertama dilakukan kontrol penyakit mastitis subklinis melalui pengujian CMT, kemudian dilakukan isolasi bakteri pada sampel susu. Tahap selanjutnya dilakukan penyuluhan mengenai pencegahan penyakit mastitis pada sapi perah serta penggunaan antiseptik dan desinfektan. Berdasarkan hasil uji CMT didapatkan bahwa empat sampel negatif dan sampel yang positif hanya sampai positif dua. Hasil pengujan isolasi bakteri menunjukkan adanya pertumbuhan koloni bakteri Escherichia coli. Sampel pada uji CMT positif tidak selalu menunjukkan adanya bakteri, begitu juga sampel yang dilakukan uji CMT dengan hasil negatif, terdapat pertumbuhan bakteri. Pengujian tersebut penting untuk dilakukan untuk Tindakan pencegahan dan pengendalian penyakit. Peternak di bendul merisi Surabaya harus menerapkan prinsif aseptis dan menggunakan desinfektan pada kandang
Profil Resistensi Antibiotik pada Isolat Aeromonas hydrophila dari Sampel Klinis Ikan Nila dan Ikan Gurame Reina Puspita Rahmaniar; Dyah Widhowati; Aditya Yoppy Ro Candra
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 4 (2025): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i4.18978

Abstract

This study aimed to determine the antibiotic resistance profile of Aeromonas hydrophila isolated from clinical samples of Nile tilapia (Oreochromis niloticus) and gourami (Osphronemus goramy) showing disease symptoms. The research method involved sampling fish organs exhibiting anatomical pathological changes, followed by bacterial isolation using MacConkey Agar. Bacterial identification was conducted through Gram staining and biochemical tests, including TSIA, SIM, MR, VP, Simmons Citrate Agar, urease, and catalase tests. Isolates identified as A. hydrophila were subsequently tested for antibiotic susceptibility using the Kirby–Bauer disk diffusion method on Mueller Hinton Agar. Eight antibiotics were evaluated, namely ciprofloxacin, tetracycline, erythromycin, cefotaxime, ampicillin, gentamicin, streptomycin, and nalidixic acid. The results showed that four A. hydrophila isolates were obtained from a total of 20 fish samples examined. Antibiotic susceptibility testing revealed that two out of four isolates (50%) were resistant to ampicillin and one isolate (25%) was resistant to streptomycin, while all isolates remained sensitive to ciprofloxacin, tetracycline, erythromycin, cefotaxime, gentamicin, and nalidixic acid. In conclusion, the presence of antibiotic resistance in A. hydrophila, particularly to certain β-lactam and aminoglycoside antibiotics, indicates the need for continuous monitoring and prudent use of antibiotics in aquaculture practices to prevent the further development and spread of resistant pathogenic bacteria.
Case Study of Pseudomonas sp. in Giant Gourami (Osphronemus goramy) via Anatomical Pathology, Histopathology, and Microbiological Examinations, and Tetracycline Sensitivity Testing Reina Puspita Rahmaniar; Indra Rahmawati; Olan Rahayu Puji Astuti Nussa; Muhammad Irwan Firmansyah
Jurnal Biologi Tropis Vol. 26 No. 3 (2026): Juli - September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v26i3.12956

Abstract

Pseudomonas sp. is an opportunistic bacterial pathogen causing systemic infections in cultured fish, resulting in economic losses and increasing concerns regarding antimicrobial resistance. However, comprehensive pathological and microbiological confirmation remains limited. This study aimed to evaluate Pseudomonas sp. infection in giant gourami (Osphronemus goramy) through pathological examination, bacterial identification, and antibiotic susceptibility testing. A descriptive case study was conducted on one naturally infected fish (code F-11). Clinical observation, necropsy, histopathological examination of the liver and gills using hematoxylin-eosin staining, bacterial isolation on MacConkey agar, Gram staining, biochemical characterization, and Kirby-Bauer disk diffusion testing with 30 μg tetracycline were performed. Data were analyzed descriptively by integrating pathological and microbiological findings. Gross examination revealed ecchymotic hemorrhages in the gills, labyrinth organ, and liver, hepatic nodules, and jejunal petechiae. Histopathology demonstrated congestion, inflammatory cell infiltration, hemorrhage, focal hepatic necrosis, epithelial desquamation, and lamellar necrosis in the gills. The isolate produced transparent non-lactose-fermenting colonies, consisted of Gram-negative rods, and exhibited biochemical characteristics consistent with Pseudomonas sp. The isolate remained susceptible to tetracycline, producing a 28-mm inhibition zone. These findings confirm Pseudomonas sp. infection and highlight the importance of integrated pathological and microbiological diagnosis to support appropriate antimicrobial therapy and antimicrobial resistance surveillance in aquaculture.
Co-Authors Aditya Yoppy Ro Candra Arif Mardjianto Candra, Adhitya Yoppy Ro Dina Wahyu Nur Aini Diningrum, Dinda Prisilya Elsa Rosita Era Hari Mudji Hana Cipka Pramuda Wardhani Indra Rahmawati Kendek, Irfan Alias Muhammad Irwan Firmansyah Ningsih, Awalul Fitria Noka, Isara Abda Nurul Hidayah Nurul Hidayah NURUL HIDAYAH Olan Rahayu Puji Astuti Nussa Ratna Widyawati Rianto, Vinsensius Sheila Marty Yanestria Trirahayu, Rizkika Amalia Wibisono, Freshinta Jellia Widhowati, Dyah Wirjaatmadja, Roeswandono