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Sinergisme aktivitas antibakteri ekstrak biji bengkuang (Pachyrhizus erosus L.) terhadap Pseudomonas aeruginosa berdasarkan bioautografi Rahminiwati, Min; Supardi, Arviani Rahmawati; Mahyuni, Siti
Current Biomedicine Vol. 2 No. 1 (2024): January
Publisher : School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/currbiomed.2.1.1-12

Background: A 70% ethanol extract of jicama seeds has been reported to have antibacterial activity against Pseudomonas aeruginosa. Phytochemical tests of the extract have identified alkaloids, flavonoids, saponins, and tannins. However, the compounds responsible for its antibacterial effects against P. aeruginosa have not yet been studied. Objective: This study was conducted to determine the class of chemical compounds responsible for the antibacterial activity against P. aeruginosa. Methods: The antibacterial activity against P. aeruginosa was studied using the minimum bactericidal concentration (MBC) of the jicama seed extract at concentrations of 6, 10, 14, 18, 22, 26, 30, and 34% in liquid and solid Nutrient Agar media, the wide inhibitory zone (WIZ) using the Kirby Bauer method, and the retention factor (Rf) of the extract using thin-layer chromatography (TLC)-bioautography. Results: The study results showed that the jicama seed extract had an MBC value of 26%, and the best WIZ value in the extract was 5.68 mm at concentration of 34%. The Rf value in the extract at concentration of 34% which showed antibacterial activity against P. aeruginosa was 0.812, 0.750, 0.675, 0.625, and 0.787 of each compound group identified as flavonoids, tannins, quinones, alkaloids, and triterpenoids. The WIZ value of each compound was lower than the WIZ value of the extract. Conclusion: Based on the Rf data, the antibacterial effect of jicama seed extract against P. aeruginosa is likely the result of a synergistic interaction between the chemical compounds contained therein.

Effectiveness of Citronella Extract (Cymbopogon nardus) Mouthwash From Microwave-Assisted Extraction Method Against Streptococcus mutans Komala, Oom; Mahyuni, Siti; Pradiningsih, Anna; Dewi, Elfrieda Kusuma
FITOFARMAKA: JURNAL ILMIAH FARMASI Vol 14, No 1 (2024): FITOFARMAKA: Jurnal Ilmiah Farmasi
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/jf.v14i1.9621

Cymbopogon nardus, or citronella has a distinctive aroma is known as a source of essential oils. Extract of citronella can be use as mouthwash to reduce bad breath and protect teeth from bacteria that cause dental caries. C. nardus contains antibacterial compounds such as flavonoid, essential oils, polyphenols, and saponin which are also act as antioxidant compounds. This research was carried out to formulate mouthwash using citronella extract and determine the antibacterial activity of the citronella mouthwash against S. mutans bacteria using disc diffusion method. The microwave extraction method (MAE) was used to obtain extract using 70 % and 96 % ethanol as a solvents. The mouthwash were made in 3 formulas i.e. formula 1, formula 2, and formula 3 each contain 25 % w/v, 30 % w/v, and 35 % w/v) respectively. The quality of mouthwash includes organoleptic, pH, viscosity and hedonic was evaluated and antibacterial activity was determined by measuring the value of minimum inhibitory concentration (MIC) and diameter of inhibition zone. The MIC value of citronella extract was achieved at concentration 20 %. All mouthwash formula met the requirements for a good mouthwash. Formula 1 was the most preferred formula by the panelists based on the hedonic test. Formula 3 had the strongest antibacterial activity with diameter inhibitory of 17.6 ± 0.52 mm. It was concluded that citronella mothwash has the potential to be developed into an antibacterial mouthwash.

The Sunscreen activities of ethanol, ethyl acetate, n-hexane, and water fractions from papaya (Carica papaya l.) leaf extract Wardatun, Sri; Mahyuni, Siti; Setiawan, Putra
Indonesian Journal of Pharmaceutics Vol 5, Issue 2, May - August 2023
Publisher : Universitas Padjadjaran (Unpad)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/idjp.v5i2.46557

The application of sunscreen products containing natural compounds is one ofalternative popular ways to protect the skin from the harmful effects of sunexposure. The aim of this study was to evaluate the sunscreen activity of papayaleaf extract. The ethanol extract of papaya leaf was fractionated to obtain ethanol,n-hexane, ethyl acetate, and water extracts. The value of SPF 15, percenttransmission of erythema (1% Te ), and the percent transmission of pigmentation40 (% Tp) of each extract were calculated in vitro by using spectrophotometricmethod. among the extract examined, the ethyl acetate extract was found to be themost effective to reach Sun Protection Factor (SPF) 15 (ultra-protection category)and 1% Te (sunblock category) at a minimum concentration of 90.08 ppm and63.49 ppm. Meanwhile n-hexane extract was the most effective to reach 40% tp(sunblock category) at a minimum concentration of 30.68 ppm. The minimumconcentration of papaya leaf extracts required to reach SPF 15, 1% Te and 40% Tpwere far below the concentration of sunscreen compounds allowed in commercialcosmetic products. It can be concluded that papaya leaf extract have significantsunscreen property for use as cosmetic ingredient.Keywords: Carica papaya, SPF, Transmission of erythema, Transmission ofpigmentation

EFEKTIVITAS SALEP EKSTRAK ETANOL 70% DAUN BANDOTAN (Ageratum conyzoides L.) TERHADAP Staphylococcus aureus dan Propionibacterium acnes Mahyuni, Siti; Almasyhuri, Almasyhuri; Sausan, Alfy Salma
INDONESIA NATURAL RESEARCH PHARMACEUTICAL JOURNAL Vol 9, No 1 (2024)
Publisher : Fakultas Farmasi Universitas 17 Agustus 1945 Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52447/inrpj.v9i1.7409

Infeksi kulit umum disebabkan oleh bakteri Staphylococcus aureus dan Propionibacterium acnes. Daun Badotan secara tradsional digunakan untuk pengobatan luka luar. Daun bandotan memiliki aktivitas sebagai antibakteri karena di dalam daun bandotan terdapat senyawa kumarin, kariofilen dan ageratokromen. Penelitian ini bertujuan untuk membuat formula salep kulit ekstrak daun bandotan yang memenuhi syarat mutu fisik berdasarkan Standar Nasional Indonesia (SNI) dan menentukan aktifitasnya terhadap S. aureus dan P. acnes. Dibuat 3 formula salep masing-masing dengan penambahan ekstrak daun bandotan sebesar 10% (F1), 15% (F2) dan 20% (F3). Dilakukan uji mutu fisik meliputi organoleptik, homogenitas, pH, daya sebar, daya lekat, viskositas dan cycling test dan aktifitas antibakteri dengan metode sumuran. Hasil uji menunjukkan semua formula salep ekstrak daun bandotan memenuhi syarat mutu fisik berdasar SNI 16-4399-1996. Sedian F1, F2, dan F2 masing-masing memiliki daya hambat terhadap S. aureus dengan rata-rata DDH 18,24 mm, 22,70 mm, dan 25,86 mm, sedangkan rata-rata DDH terhadap P. acnes adalah 19,10 mm, 23,17 mm, dan 25,47 mm. Disimpulkan bahwa salep ekstrak etanol 70 % daun bandotan berpotensi dikembangkan sebagai obat infeksi kulit yang disebabkan S. aureus dan P. acnes.

Efektivitas Ekstrak-Campuran Simplisia Dibandingkan Dengan Campuran Ekstrak Berdasarkan Aktivitas Antioksidan dan Kapasitas Antioksidan Rusli, Zaldy; Mahyuni, Siti; Maisaroh, Siti
Jurnal Farmasi Ma Chung: Sains, Teknologi, dan Klinis Komunitas Vol. 2 No. 1 (2024): Jurnal Farmasi Ma Chung: Sains, Teknologi, dan Klinis Komunitas
Publisher : Program Studi Farmasi, Universitas Ma Chung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33479/jfmc.v2i1.28

Tanaman Indonesia banyak yang berpotensi sebagai antioksidan. Untuk mendapatkan suatu bahan yang kaya akan antioksidan, maka tumbuhan tersebut dibuat menjadi ekstrak, untuk selanjutnya diproses lebih lanjut. Tujuan dari penelitian ini untuk membandingkan efektivitas dari 2 macam ekstrak, yaitu ekstrak dari campuran 2 simplisia dan kombinasi dari ekstrak masing-masing simplisia. Parameter yang diukur adalah aktivitas antioksidan. Pengujian aktivitas antioksidan menggunakan metode pengukuran penangkapan radikal bebas 1,1-difenil-2-pikrilhidrazil (DPPH). Ekstraksi dilakukan dengan cara maserasi. Berdasarkan aktivitas antioksidan didapatkan aktivitas antioksidan dari ekstrak campuran (IC50 67,059 ppm) lebih baik dibandingkan kombinasi ekstrak (92,419). Hasil ini menunjukkan bahwa efektivitas produksi akan meningkat, karena hanya satu tahap, yaitu mencampurkan beberapa simplisia untuk dijadikan ekstrak akan lebih efektif jika dibandingkan ekstraksi beberapa simplisia (beberapa tahapan) untuk kemudian dicampurkan.

THE POTENCY OF PSIDIUM GUAJAVA LEAVES AS A NATURAL DISINFECTANT AND ANTISEPTIC INGREDIENT Mahyuni, Siti; Komala, Oom; Wiendarlina, Ike Yulia; Fatah, Muhammad Fajar Saeful
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 1 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.9216

Preventing the spread of nosocomial infection are generally carried out by disinfection process of the tools to be used with chemical disinfectant such as phenol, alcohol, chlorine, iodine or sublimate. Excessive use of chemicals can cause health problems. Guava leaves (Psidium guajava L.) have long been empirically used to treat diarrhea and acne. Scientific data shows that guava leaves have positive activity against several types of pathogenic bacteria due to its tannin content. Guava leaves have the potential to be developed as a disinfectant and antiseptic material. This study aims to determine the disinfection activity of guava leaves on surgical instruments using the total plate count method, skin irritation test using patch test method and quantify the tannin content in young and old guava leaves using spectrophotometry method. The results showed that the tannin content in old and young guava leaves was 68.73 ± 0.067 mg/g (6.873%) and young 61.87 ± 0.046 mg/g (6.187%). At concentrations of 10, 20, 30 and 40% guava leaf extract were able to reduce the total plate count in surgical instrument samples by 6.85; 37; 80.82; and 83.56%. The primary irritation index of 0.05 only occurs at a concentration of 40%, classified as non-irritant. It can be concluded that guava leaves have the potential to be further developed as a natural disinfectant or antiseptic.

Aktivitas Ekstrak Microwave Assisted Extraction dan Refluks Daun Alamanda (Allamanda cathartica L.) terhadap Trychophyton rubrum Penyebab Dermatofitosis Mahyuni, Siti; Komala, Oom; Fadilah, Nurul
FASKES : Jurnal Farmasi, Kesehatan, dan Sains Vol. 3 No. 1 (2025): Bulan Juli 2025, FASKES : Jurnal Farmasi, Kesehatan, dan Sains
Publisher : Program Studi Farmasi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32665/faskes.v3i1.3419

Latar belakang: Daun alamanda (Allamanda cathartica L.) secara empiris dikenalbersifat antijamur, pencahar, pereda batuk, penawar racun, pereda demam.Tanaman alamanda memiliki potensi besar sebagai antijamur Trichophyton rubrumyang sering menginfeksi kulit dan kuku manusia.Tujuan penelitian: menganalisisaktivitas antijamur ekstrak Microwave Assisted Extraction (MAE) dan refluks daunalamanda terhadap jamur T. Rubrum Metode: Aktivitas antijamur ditentukandengan menguji nilai Konsentrasi Hambat Minimum (KHM) menggunakan metodedilusi padat pada konsentrasi 2, 5, 7, dan 10 % dan nilai Diameter Daya Hambat(DDH) dengan metode difusi cakram pada konsentrasi 10, 12,5, 15 dan 17,5 %.Hasil: Ekstrak hasil MAE dan Refluks memiliki aktivitas antijamur T. rubrumdengan nilai Konsentrasi Hambat Minimum (KHM) yang sama yaitu padakonsentrasi 10 %. Nilai DDH ekstrak MAE dan ekstrak refluks pada konsentrasi10 %, 12,5 %, 15 %, dan 17,5 % yaitu 13,66 ±0,76; 14,25 ±0,97; 16,24±0,29 dan19,34±0,99 mm pada ekstrak MAE dan 10,34±0,29; 14,16±0,11; 14,66±0,96 dan16,00 ±0,12 mm pada ekstrak refluks. Simpulan dan saran: Aktifitas anti T.rubrum ekstrak MAE lebih tinggi diandingkan ekstrak refluks. Perlu dilakukanfraksinasi dan identifikasi senyawa aktif antijamur pada tanaman alamanda.

Training on Making Herbal Drink to Increase Immunity in Pagelaran Village, Ciomas, Bogor Tri Retno Handayani, Marybet; Yulianita; Wiendarlina, Ike Yulia; Utami, Novi Fajar; Mahyuni, Siti; Sulistiyono, Fitria Dewi; Komala, Oom; Hermawati, Ema; Rikkit
Jurnal Pengabdian Masyarakat Inovatif Vol. 1 No. 2 (2023): JPMI (Jurnal Pengabdian Masyarakat Inovatif)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/jpmi.v1i2.79

Health is a very valuable factor in life. One way to improve health or immunity is by consuming herbal drinks. Herbal drinks are believed to have beneficial properties for healing disease. Livelihoods in Pagelaran Village are dominated by laborers, farmers, traders and domestic workers, with educational levels dominated by junior high and high school levels. The problems found in Pagelaran Village are public health problems, so it is necessary to improve welfare, one of which is by consuming healty drinks made from herbal plants. Red ginger is widely used to treat various diseases such as colds, digestive disorders, antipyretic, anti-inflammatory and also analgesic. Compounds of lemongrass that have a good prospects for increasing immune system activity are usually flavonoids, curcumin, limonoids, vitamin C, vitamin E (tocopherol) and catechins. Lemons also contain quercetin which can increase the body's immunity. This PKM activitiy was carried out in Agroeduwisata Lembah Watu, Pagelaran Village, Bogor Regency, took the form of training in making herbal drinks to increase body’s immunity for women of PKK and KWT. The implementation of this PKM activity can increase the knowledge of utilizing, processing and packaging herbal plants into herbal drink products. This PKM activity, it is hoped that people will be able to make and consume health drinks which can be used to increase the body’s immunity so that the quality of life can improves too.

Training on Making Herbal Drink to Increase Immunity in Pagelaran Village, Ciomas, Bogor Tri Retno Handayani, Marybet; Yulianita; Wiendarlina, Ike Yulia; Utami, Novi Fajar; Mahyuni, Siti; Sulistiyono, Fitria Dewi; Komala, Oom; Hermawati, Ema; Rikkit
Jurnal Pengabdian Masyarakat Inovatif Vol. 1 No. 2 (2023): JPMI (Jurnal Pengabdian Masyarakat Inovatif)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/jpmi.v1i2.79

Health is a very valuable factor in life. One way to improve health or immunity is by consuming herbal drinks. Herbal drinks are believed to have beneficial properties for healing disease. Livelihoods in Pagelaran Village are dominated by laborers, farmers, traders and domestic workers, with educational levels dominated by junior high and high school levels. The problems found in Pagelaran Village are public health problems, so it is necessary to improve welfare, one of which is by consuming healty drinks made from herbal plants. Red ginger is widely used to treat various diseases such as colds, digestive disorders, antipyretic, anti-inflammatory and also analgesic. Compounds of lemongrass that have a good prospects for increasing immune system activity are usually flavonoids, curcumin, limonoids, vitamin C, vitamin E (tocopherol) and catechins. Lemons also contain quercetin which can increase the body's immunity. This PKM activitiy was carried out in Agroeduwisata Lembah Watu, Pagelaran Village, Bogor Regency, took the form of training in making herbal drinks to increase body’s immunity for women of PKK and KWT. The implementation of this PKM activity can increase the knowledge of utilizing, processing and packaging herbal plants into herbal drink products. This PKM activity, it is hoped that people will be able to make and consume health drinks which can be used to increase the body’s immunity so that the quality of life can improves too.

MICROBIAL BIOCONVERSION TO PRODUCE NUTRACEUTICAL AND PHARMACEUTICAL BIOACTIVE COMPOUNDS Mahyuni, Siti
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 1 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2022.1793

he bioconversions of bioactive health supporting compounds using certain microorganisms have long been known, however it has not been widely applied in industrial scale to produce nutraceutical and products of high economic value. This review article will discuss the group of steroids, carotenoids and statins which were produced by microbial bioconversion using the various substrates and conditions. The information presented in this article were collected from scientific books and articles in national and international journals which can be validated and traced back to their sources through many sites such as google.com, google scholar, NCBI and Science Direct. From the information compiled in this article, it can be concluded that the bioconversion of bioactive compounds by microorganisms has shown positive results and potential to be further optimized and applied in an industrial scale to produce natural bioactive compounds as basic ingredients for nutraceutical and pharmaceutical products with high economic value.

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