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All Journal Buletin Peternakan Jurnal Peternakan Indonesia Livestock and Animal Research Jurnal Aplikasi Teknologi Pangan Tropical Animal Science Journal Jurnal Aplikasi Teknologi Pangan Journal of Tropical Animal Science and Technology Jurnal Agripet
Wihandoyo, Wihandoyo
Fakultas Peternakan, Universitas Gadjah Mada, Yogyakarta
Agriculture, Biological Sciences & Forestry Chemical Engineering, Chemistry & Bioengineering Chemistry Energy Immunology & microbiology Veterinary Other

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Journal : Jurnal Agripet

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Aktivitas Antibakteri Bakteri Asam Laktat (BAL) yang Diisolasi dari Saluran Pencernaan Itik Lokal Asal Aceh terhadap Salmonella pullorum dan Escherichia coli Yayuk Kurnia Risna; Sri Harimurti; Wihandoyo Wihandoyo; Widodo Widodo; Ari Surya Sukarno
Jurnal Agripet Vol 22, No 2 (2022): Volume 22, No. 2, Oktober 2022
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v22i2.24297

Abstract

ABSTRACT. Penelitian ini bertujuan untuk melihat aktivitas antibakteri dari bakteri asam laktat (BAL) yang sudah diisolasi dari saluran pencernaan itik lokal asal Aceh. Berdasarkan hasil identifikasi gen 16S rRNA menggunakan metode PCR dan Sekuensing diperoleh lima BAL yaitu Lactobacillus plantarum strain C1; Pediococcus acidilactici strain V2; Lactobacillus fermentum Strain D3; Lactobacillus fermentum Strain I1; Lactobacillus fermentum Strain S4. Penelitian ini mengukur kemampuan aktivitas lima BAL terhadap Salmonella pullorum dan Escherichia coli yang dilakukan dengan difusi (teknik sumuran). Uji ketahanan terhadap kondisi asam (pH 2) dan ketahanan terhadap garam empedu (bile salt) dilakukan dengan metode Total Plate Count (TPC) menggunakan medium agar MRS metode pour plate. Hasil penelitian diperoleh bahwa Pediococcus acidilactici strain V2 memiliki aktivitas antimikroba terbaik terhadap bakteri Salmonella pullorum dan Escherichia coli. Pediococcus acidilactici strain V2 juga memberikan hasil terbaik pada ketahanan terhadap pH 2 dan garam empedu 1%.(Antibacterial activity of LAB isolated from the digestive tract of a native Aceh duck of Salmonella Pullorum and Escherichia coli)ABSTRAK. This study aimed to examine the antibacterial activity of lactic acid bacteria isolated from digestive tract of Local ducks from Aceh. Identification of the 16S rRNA gene using PCR and sequencing methods were obtained five LAB is Lactobacillus plantarum strain C1; Pediococcus acidilactici strain V2; Lactobacillus fermentum Strain D3; Lactobacillus fermentum Strain I1; and Lactobacillus fermentum Strain S4. This study measure the ability five BAL activity test against Salmonella pullorum and Escherichia coli by diffusion (well technique). Test of resistance to acid conditions (pH2) and to bile salt were using the Total Plate Count (TPC) method with MRS Agar medium by pour plate. The results showed that Pediococcus acidilactici strain V2 had the best antimicrobial activity against Salmonella pullorum and Escherichia coli. Pediococcus acidilactici strain V2 also gave the best results on resistance to pH 2 and 1% bile salt.
Pengaruh Bungkil Inti dan Lumpur Sawit yang Difermentasi dengan Aspergillus sp asal Akar Bambu terhadap Kandungan Lemak Ayam Broiler Bambang Hartoyo; Supadmo Supadmo; Wihandoyo Wihandoyo; Ali Wibowo
Jurnal Agripet Vol 15, No 2 (2015): Volume 15, No. 2, Oktober 2015
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v15i2.2850

Abstract

(The effect of palm kernel cake and palm oil sludge fermented with aspergillus sp derived from bamboo root on broilers fat content)ABSTRACT. The objective of this research was to determine the effect of palm kernel cake and palm oil sludge fermented with Aspergillus sp derived from bamboo root on broilers fat content including liver fat, abdominal fat and meat fat. Study was conducted for 8 weeks in Experimental Farm, Animal Science Faculty Jenderal Soedirman University, Purwokerto. Research utilized 196 male DOC strain Lohman, ration treatments, cage and other utilities. Seven allotted rations were R0 = control ration (without FPKC and FPOS), R1 = 7.5% FPKC, R2 = 15% FPKC, R3 = 22.5% FPKC, R4 = 7.5% FPOS, R5 = 15% FPOS, R6 = 22.5% FPOS. Each treatment unit used 7 (seven) DOCs with 4 (four) replicates. The obtained data were subject to analysis of variance followed by Orthogonal Contrasts. Result demonstrated that liver fat level was 1,79 3,86%, abdominal fat was 0,52 2,04%, and meat fat was 0,21 0,61%. Analysis of variance result showed that supplementing palm kernel cake and palm oil sludge fermented with Aspergillus sp derived from bamboo root highly significantly affected (P 0.01) abdominal fat level, significantly affected (P 0.05) liver fat level but did not significantly affected (P 0.05) broiler meat fat level.
Co-Authors Ali Wibowo Ari Kusuma Wati Ari Surya Sukarno B. L. Syaefullah Bagus Andika Fitroh Bambang Hartoyo Cahya Setya Utama Chusnul Hanim Dyah Maharani Galuh Adi Insani Kustantinah Kustantinah Lisnahan, Charles V. Muhlisin Muhlisin N. A. Fitriyanto N. Kurniawati Nanung Danar Dono R. A. Prasetyo R. Edwin Indarto Sri Harimurti Sri Harimurti Sri-Harimurti Sri-Harimurti Stefanie Silvia Supadmo Supadmo Supadmo Supadmo Vellina Putri Handayani Widodo Widodo Widodo Widodo Y. Erwanto Yayuk Kurnia Risna Zuprizal (Zuprizal)