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All Journal Journal of Tropical Soils Jurnal Agrotek Tropika Akta Agrosia Jurnal Agrotropika Indonesian Journal of Community Services Cel Jurnal Pengabdian Fakultas Pertanian Universitas Lampung Jurnal Pengabdian Masyarakat dan Penelitian Terapan
Ramadiana, Sri
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Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Environmental Science Social Sciences Other

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Journal : Jurnal Agrotropika

 1 
RESPON PERTUMBUHAN TUNAS DARI PROTOCORM-LIKE BODIES MENJADI PLANLET ANGGREK DENDROBIUM HIBRIDA IN VITRO TERHADAP DUA JENIS MEDIA DAN PEMBERIAN TRIPTON Zasari, Maera; Ramadiana, Sri; Yusnita, Yusnita; Hapsoro, Dwi
JURNAL AGROTROPIKA Vol 15, No 1 (2010): Agrotropika Vol.15 No.1 2010
Publisher : JURNAL AGROTROPIKA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (258.501 KB)

Abstract

Dendrobium is great interest because of its high value as a floricultural commodity and a kind of elite herbage plant in Indonesia. An efficient in vitro propagation system via protocorm-like body (PLBs) induction has been developed using one-year-old axillary node explants. The potential of different media and supplements triptone for reliable Dendrobium orchid plantlet regeneration from in vitro PLBs explants were studied. In all two media under investigation, shoot regeneration was achieved when the PLBs were transferred onto solidified MS half strenght and Growmore medium with or without 2 g L-1 triptone supplemented with 15% (v/v) coconut water, 20 g L-1 sucrose, and 2 g L-1 charcoal. The shoots development investigation after three month of culture. 1/2 MS and Growmore media significantly influenced the plantlet regeneration of Dendrobium orchid. Among the media, Growmore showed better performance on shoots and leaves per plantlet, but triptone showed no significant effect on number shoots and leaves. The longest shoots were found in Growmore medium without triptone. Key words: Dendrobium, in vitro, PLBs, planlet, triptone.
Pengaruh Pemberian Dosis Pupuk NPK dan Jenis Pupuk Hayati terhadap Pertumbuhan dan Produksi Tanaman Bawang Merah (Allium ascalonicum L.) Hendarto, Kus; Widagdo, Setyo; Ramadiana, Sri; Meliana, Fitria Sita
JURNAL AGROTROPIKA Vol 20, No 2 (2021): Oktober 2021
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/ja.v20i2.5086

Abstract

Shallots (Allium ascalonicum L.) is one of the leading vegetable commodities used as food seasoning and traditional medicine. This study aims to see the effect of providing various doses of NPK fertilizer and types of biological fertilizers on the growth and production of shallot plants. This study used a randomized block design (RAK) arranged in a factorial (4 x 2). The first factor is the NPK fertilizer dosage which consists of four levels, namely: N0= 0% (without NPK fertilizer), N1= 50% (25 g/m2), N2= 100% (50 g/m2), and N3= 150% (75 g/m2). The second factor is the type of biological fertilizer which consists of two levels, namely: H1= Grikulan plus fertilizer and H2= EM4. The results showed that the NPK fertilizer dosage treatment up to a dose of 75g/m2 gave the best results on plant growth and development, including the number of leaves, plant height, fresh tuber weight per plant, wind dry tuber weight per plant, and wind dry tuber weight per m2. The growth and development of shallot plants with the application of Grikulan biological fertilizers were better than those of EM4 biological fertilizers. Treatment of NPK fertilizer dosage of 150% or equivalent to 75 g / m2 supplemented with the application of Grikulan plus fertilizer results in higher production, namely the weight of dry tubers per m2 reaching 1674.33 g or equivalent to 11.72 tonnes/ha. Keywords : EM4, Grikulan plus, shallots,NPK, yield
SCALP AND SHOOT FORMATION IN RESPONSE TO THIDIAZURON CONCENTRATIONS IN VITRO CULTURE OF PLANTAIN ‘TANDUK’ Hapsoro, Dwi; Prayogi, Adi Noor; Ramadiana, Sri; Yusnita, Yusnita
JURNAL AGROTROPIKA Vol. 24 No. 1 (2025): Jurnal Agrotropika Vol 24 No 1, Mei 2025
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/ja.v24i1.9927

Abstract

This experiment aimed to study the formation of scalps and  banana shoots 'Tanduk' in vitro in response to thidiazuron (TDZ). Shoot explants measuring ± (1 x 1 x 1) cm3 were cultured in precondition media (MS+ 5 mg/l benzyladenine) for 4 weeks, then transferred to treatment media, namely MS + TDZ. This experiment was carried out using a completely randomized design with 3 replications and 7 treatments of TDZ concentrations (0.5; 1.0; 1.5; 2; 2.5; 3; 3.5; 4.0 mg/l).  Each experimental unit consisted of 5 bottles, each containing one explant. Observations of scalp number were carried out at 8 weeks of age. The scalps were subcultured on MS+5mg/l BA media to induce shoots. After 8 weeks in the shoot induction medium, the number of shoots was recorded.  The results showed that at 8 weeks on the culture all of the explants formed scalps, with the highest number of scalps (6 scalps/explant) was at 1 mg/l TDZ.  The scalp number decreased to 4.4 to 3.5/explant as the TDZ concentration increased from 1.5 to 4 mg/l. After 8 weeks on shoot induction media containing 5 mg/l BA, the highest number of shoots (3.8 shoots/explant) was obtained on the scalp from the 0.5 mg/l TDZ treatment. The shoot number decreased to (3.2-1.9/explant) with increasing TDZ concentrations from 1.0 to 4.0 mg/l.Keywords: Tanduk, plantain, thidiazuron, in vitro, scalp.
EXTRACT OF MORINGA LEAF AND SEAWEED AS BIOSTIMULANT FOR INCREASING SUCCESS AND GROWTH OF COCOA (Theobroma cacao L.) SHOOT GRAFTING Ramdani, Muhammad Rizki; Evizal, Rusdi; Wibowo, Lestari; Ramadiana, Sri
JURNAL AGROTROPIKA Vol. 24 No. 2 (2025): Jurnal Agrotropika Vol 24 No 2, Oktober 2025
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/ja.v24i2.11102

Abstract

Cocoa (Theobroma cacao L.) is a plantation crop that contributes to Indonesia’s economy through export value and serves as the main source of income for farmers in major production centers. Grafting technology is widely used to increase productivity; however, its success rate remains low. The use of biostimulants derived from moringa leaf extract and seaweed extract offers an alternative solution to improve the success and growth of cocoa grafts. This study aimed to determine the effect of type and concentration of biostimulants on the success and growth of cocoa grafts. The research was conducted from December 2024 to February 2025 in Labuhan Dalam, Tanjung Senang District, Bandar Lampung City. A non-factorial randomized complete block design (RCBD) was used, consisting of 7 treatments and 3 replications. The treatments included a control (0 ml/l), moringa leaf extract at 150 ml/l, 300 ml/l, and 450 ml/l, as well as seaweed extract at 150 ml/l, 300 ml/l, and 450 ml/l. Data were analyzed using ANOVA and tested with the Least Significant Difference (LSD) at a 5% significance level. The results showed that the application of biostimulants had a significant effect on the time of shoot emergence, number of shoots, shoot length, shoot diameter, and leaf greenness. However, the treatments did not significantly affect the number of leaves, number of flushes, fresh weight of shoots, or dry weight of shoots. All biostimulant applications resulted in a 100% grafting success rate. The best concentration of both moringa leaf and seaweed biostimulant extracts was 300 ml/l. Key words: Biostimulants, moringa leaves, seaweed, shoot grafting
Co-Authors Agus Karyanto Darwin H. Pangaribuan DWI HAPSORO Edy, Akari Ermawati Ermawati Ermawati Fortuna, Kirana Ceri Hayane Adeline Warganegara, Hayane Adeline Herry Susanto Hidayat Pujisiswanto Kus Hendarto, Kus Lestari Wibowo Maera Zasari Meliana, Fitria Sita Pratiwi, Bela Ayu Prayogi, Adi Noor Raden Ajeng Diana Widyastuti Ramdani, Muhammad Rizki Rusdi Evizal Sanjaya, Purba Setyo Widagdo Suprihatin Suprihatin Widyastuti , RA. Diana Widyastuti, R A Diana Widyastututi, RA Diana YUSNITA YUSNITA