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All Journal BIOEDUKASI Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan JURNAL FARMASIMED (JFM) Indonesia Natural Research Pharmaceutical Journal (INRPJ) Pharmacy Action Journal KAMI MENGABDI Indonesian Journal of Pharmaceutical Research
Panjaitan, Riong seulina
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Library & Information Science Medicine & Pharmacology Public Health Social Sciences Transportation Other

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Journal : BIOEDUKASI

 1 
Ultrasound Assisted Extraction (UAE) of Macroalgae Padina australis From Tidung Island, Indonesia and Testing Its Antibacterial Activity Yosepin, Elpina; Panjaitan, Riong Seulina; Purwati, Purwati
BIOEDUKASI Vol 22 No. 3 (2024)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v22i3.50081

Abstract

Padina australis is one of brown macroalgae that grows in Indonesian ocean. This study aimed to determine the optimum of time extraction of Padina australis ethanolic extract extracted by Ultrasound Assisted Extraction (UAE) method, its phytochemical compounds and the antibacterial activity of Padina australis ethanolic extract against on four different pathogenic bacteria (Salmonella typhii, Shigella sonnei, Escherichia coli dan Bacillus cereus). Padina australis were extracted by Ultrasound Assisted Extraction (UAE) method using ethanol solvent (70%) with three different times (10, 20 and 30 minutes). The ethanolic extract of Padina australis were evaluated its antibacterial activity and its minimal inhibitory concentration (MIC) by using disc diffusion method. The results showed that the optimum UAE time of Padina australis was 20 minutes which yielded 0.4%. Moreover, the ethanolic extract of Padina australis contained alkaloids, saponins, flavonoids, tannins, steroids, and terpenoids. Furthermore, this study indicates that Padina australis ethanolic extract demonstrated its antibacterial activity against all bacteria tested. MICs of Padina australis ethanolic extract determined for S. typhi and B. cereus were at 6.25 ppm and for S. sonnei was at 12,5 ppm. Whereas, this extract showed the MIC at 25 ppm on E. coli.
Extraction, Phytochemical Profiling, and Antibacterial Activity of n-Hexane Crude Extracts of Macroalgae Padina australis Lenoera, Agnes; Panjaitan, Riong Seulina; Purwati, Purwati
BIOEDUKASI Vol 22 No. 3 (2024)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v22i3.51123

Abstract

Padina australis, brown macroalgae, has potential bioactive compounds that possess remarkable bioactivities including antimicrobial. The present study extracts macroalgae P. australis using Ultrasound assisted extraction (UAE) with n-hexane solvent, followed by screening phytochemical compounds. Moreover, the antimicrobial activity and MIC value of the n-hexane crude extracts of Padina australis was tested against Shigella sonnei, Escherichia coli, Salmonella typhi, and Bacillus cereus by using the disc diffusion method. This result demonstrates the optimum time of UAE process is at 10 minutes that yields 0.117 %. The phytochemical screening of n-hexane crude extracts of Padina australis macroalgae revealed the presence of secondary metabolites such as alkaloids, flavonoids, saponins, tannins, steroids and phenols. Antibacterial activity of this extract against Shigella sonnei, Escherichia coli, Salmonella typhi, and Bacillus cereus at 100 ppm concentration with inhibitory diameter range of 10.97 to 13.86 mm. Then, the n-hexane crude extracts of P. australis is potent against Bacillus cereus with MIC value of 6.25 ppm.
Co-Authors Afikah, Hana Tri Anastasya, Millenia Putri Andrian, Rahmat Ardian, Kristian Derius Aslawiyah, Siti Rabiaatul Asriyanti, Laila Safa Djohansah, Violetta Elawati, Nur Febriani, Ike Ghassani, Fatiha Azka Hafiz, Muhammad Ariqoh Langit, Vinsensia Asahi Sulan Lenoera, Agnes Meze, Maria Fortunata Nurwijayanti parma, syafriadi kurnia Pradipta, Oki Purwati Purwati Putranto, Haidar Dwi Putri, Hervita Reza, Fakhrur Sabrina, Rorina Sagala, Zuraida Salsabilla, Zuli Astuti Septiyani, Anisah Simanjuntak, Yulia Verawati Simatupang, Angel Maria Sinambela, Rio Pandi Siti Aisyah Suciati, Lily Sumantri Sumantri Suryo, Nabila Dwi Wijaya, Bagas Eva Yosepin, Elpina Yulius Evan Christian Zuraida Sagala