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In vitro evaluation of the effect of Pluchea indica extracts in promoting glucose consumption activity on a liver cell line Suriyah, Wastuti H; Ichwan, Solachuddin J A; Kasmuri, Abdul R; Taher, Muhammad
Makara Journal of Health Research Vol. 23, No. 1
Publisher : UI Scholars Hub

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Background: Type 2 diabetes mellitus is a chronic metabolic disorder that is associated with mortality and morbidity. Recently, several plant-based agents have been used in the management of diabetes. Pluchea indica has been traditionally consumed as a medicinal plant in Southeast Asia, and its leaves have demonstrated induction of hypoglycemic effect in normal rats. This in vitro study aimed to evaluate the potency of P. indica extracts in stimulating glucose consumption in human liver CCL-13 cell line model. Methods: P. indica leaves were dried and extracted using a series of organic solvents and water. The effect of the extracts on cell viability was determined by MTT assay. The glucose consumption was analyzed using glucose oxidase method. Results: Our results revealed that the methanol extract of P. indica could significantly increase glucose consumption of cells in a concentration-dependent manner, which suggests the usefulness of the extract as an antidiabetic candidate via stimulation of glucose uptake into the liver cells. Conclusion: Our study suggests that P. indica is a potential natural candidate for diabetes mellitus management.

Cytotoxic and Anti-Inflammatory Activities of Garcinia xanthochymus Extracts on Cell Lines Hamidon, Hanisuhana; Taher, Muhammad; Jaffri, Juliana M; Tg Zakaria, Tg M; Sulaiman, Wan MAW; Susanti, Deny; Ichwan, Solachuddin JA; Zakaria, Zainul A
Makara Journal of Health Research Vol. 20, No. 1
Publisher : UI Scholars Hub

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Background: Garcinia xanthochymus extract has been reported to have several pharmacological properties. This study was conducted to evaluate cytotoxic and anti-inflammatory activities of G. xanthochymus extracts on cell lines. Methods: The roots and stem barks of plant were extracted using maceration method with n-hexane, dichloromethane and methanol, successively. Cytotoxic activity of the extracts was tested against MCF-7 breast adenocarcinoma using MTT assay. Anti-inflammatory study was evaluated using RAW 264.7 mouse macrophage cells. The nitric oxide production in LPS-stimulated cells was measured using Griess reagent. Results: The results of cytotoxic and anti-inflammatory study showed that dichloromethane and n-hexane extracts of root and stem bark exhibited cytotoxic activity in dose-dependent manner. Meanwhile, for anti-inflammatory study, all root extracts together with stem bark dichloromethane and n-hexane extracts reduce NO production in LPS-stimulated cells in dose dependent manner. Conclusions: This finding indicated that G. xanthochymus extracts might become interesting candidate for treatment of cancer and inflammation.

The Cytotoxic Activity of Marine Sponge-Derived Fungus Aspergillus nomius NC06 Against HT29 Colon Cancer Cells: Cytotoxic Activity of A. nomius NC06 Against HT29 Cancer Cells Artasasta, Muh. Ade; Djamaan, Akmal; Yanwirasti, Yanwirasti; Taher, Muhammad; Djamaludin, Heder; Siswanto, Siswanto; Handayani, Dian
Journal of Tropical Life Science Vol. 14 No. 1 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.01.05

The study of natural products from marine-derived fungi has been interesting tense to researchers as drug discovery sources. Marine fungus from West Sumatera, Indonesia repeatedly showed their potential for cytotoxic and antimicrobial activities. This study aims to determine the cytotoxic activity against HT29 colon cancer cells of each fraction of ethyl acetate extracts from Aspergillus nomius NC06 derived from marine sponge Neopetrosia chaliniformis. A. nomius was cultivated with rice as a growth medium and extracted with ethyl acetate solvent and evaporated in vacuo to obtain ethyl acetate extract. Furthermore, the compounds of ethyl acetate extract were separated with the VLC (Vacuum Liquide Chromatography) method. Five fractions were obtained, which further investigated their cytotoxic activity against HT29 colon cancer cells by using an MTT assay. The result showed that fractions I and III were categorized as potential fractions due to their IC50 value of 13.12 ± 0.39 μg/mL and 2.59 ± 0.19 μg/mL, respectively. It was also supported by ANOVA to measure the effect of each concentration (0.1; 1; 10; 100 μg/mL) of each fraction on the viability percentage of HT29 cells with p < 0.005.

Formulation and Characterization of Solid Self Nano Emulsifying Drug Delivery System (S-SNEDDS) Loading Curcuma xanthorrhiza Extract Syukri, Yandi; Seran, Yuvina; Hannie Fitriani; Ramadhani, Arba Pramundita; Taher, Muhammad
JSFK (Jurnal Sains Farmasi & Klinis) Vol 11 No 3 (2024): J Sains Farm Klin 11(3), December 2024
Publisher : Fakultas Farmasi Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jsfk.11.3.169-178.2024

Curcuma xanthorrhiza has an active component xanthorrhizol that is poorly soluble in water, resulting in limited absorption and bioavailability. Liquid Self Nano Emulsifying Drug Delivery System (SNEDDS) dosage formulations can improve its solubility and absorption due to its small particle size. Solid SNEDDS technology significantly overcomes some of problems in liquid SNEDDS preparations to improve the stability of liquid formulations. This study aims to formulate and characterize Curcuma xanthorrhiza S-SNEDDS preparation. SNEDDS was characterized by particle size and thermodynamic tests. Method of making S-SNEDDS uses two ways, absorption of porous compounds and spray drying. S-SNEDDS were characterized by testing infrared spectra, X-ray diffraction, scanning electron microscopy, microbial contamination and particle flow properties. Optimal S-SNEDDS formulation with 2 formulas namely F7 and F11 mannitol carrier. FTIR testing showed no interacting compounds. SEM and X-ray diffraction testing of F7 showed that the preparation was semicrystalline. While F11 described the preparation as much more crystalline due to the high content of mannitol. The microbial contamination test detected no microorganism compounds. In addition, the particle flow properties obtained a result of 02.53 seconds with a height of 24.95 mm. It is concluded that the S-SNEDDS formula F7 of Curcuma xanthorrhiza has semicrystalline preparation criteria.

The Cytotoxic Activity of Marine Sponge-Derived Fungus Aspergillus nomius NC06 Against HT29 Colon Cancer Cells: Cytotoxic Activity of A. nomius NC06 Against HT29 Cancer Cells Artasasta, Muh. Ade; Djamaan, Akmal; Yanwirasti, Yanwirasti; Taher, Muhammad; Djamaludin, Heder; Siswanto, Siswanto; Handayani, Dian
Journal of Tropical Life Science Vol. 14 No. 1 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.01.05

The study of natural products from marine-derived fungi has been interesting tense to researchers as drug discovery sources. Marine fungus from West Sumatera, Indonesia repeatedly showed their potential for cytotoxic and antimicrobial activities. This study aims to determine the cytotoxic activity against HT29 colon cancer cells of each fraction of ethyl acetate extracts from Aspergillus nomius NC06 derived from marine sponge Neopetrosia chaliniformis. A. nomius was cultivated with rice as a growth medium and extracted with ethyl acetate solvent and evaporated in vacuo to obtain ethyl acetate extract. Furthermore, the compounds of ethyl acetate extract were separated with the VLC (Vacuum Liquide Chromatography) method. Five fractions were obtained, which further investigated their cytotoxic activity against HT29 colon cancer cells by using an MTT assay. The result showed that fractions I and III were categorized as potential fractions due to their IC50 value of 13.12 ± 0.39 μg/mL and 2.59 ± 0.19 μg/mL, respectively. It was also supported by ANOVA to measure the effect of each concentration (0.1; 1; 10; 100 μg/mL) of each fraction on the viability percentage of HT29 cells with p < 0.005.

DEVELOPMENT OF LABRASOL®-BASED SNEDDS FOR ENHANCED DELIVERY OF COMBINATION PROPOLIS, SEA CUCUMBER, AND CENTELLA ASIATICAEXTRACTS Fitriani, Hannie; Syukri, Yandi; Taher, Muhammad
Jurnal Farmasi Sains dan Praktis Vol 11 No 3 (September-December 2025)
Publisher : Universitas Muhammadiyah Magelang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31603/pharmacy.v11i3.14470

The therapeutic efficacy of natural extracts such as propolis, sea cucumber or gamat (Stichopus hermanii), and pegagan (Centella asiatica) is supported by their anti-inflammatory, antioxidant, and regenerative properties. However, their pharmaceutical application is limited due to poor solubility and low bioavailability. This study aimed to develop and characterize a Self-Nanoemulsifying Drug Delivery System (SNEDDS) using Labrasol to enhance the delivery and stability of a combination of these three extracts, that exhibit synergistic effects as tissue regeneration, antimicrobial, anti-inflammatory, and antioxidant properties. SNEDDS formulations were prepared by a high energy method with Labrasol, Tween 80, and propylene glycol. The formulations were evaluated for droplet size, polydispersity index (PDI), zeta potential, and thermodynamic stability test. The optimized SNEDDS exhibited nano-sized droplets (151.13 nm ± 17.26) with low PDI (0.39 ± 0.01) and zeta potential (-50.30 ± 1.04) within the stable range, indicating a uniform, physically stable nanoemulsion, and passed the thermodynamic stability test. In conclusion, the optimized SNEDDS formulation demonstrated nano-sized droplets with low PDI and stable zeta potential, indicating good physical stability.

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