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Pembuatan Film Balutan Primer Untuk Luka Bakar yang Mengandung Kolagen Kulit Ikan Gabus (Channa striata) R, Rahmi Nofita; Ben, Elfi Sahlan; Yanwirasti, Yanwirasti; Aldi, Yufri
Jurnal Sains dan Teknologi Farmasi Vol 19 No Supl1 (2017): Vol 19 Supplement 1, December 2017
Publisher : Fakultas Farmasi Universitas Andalas

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Balutan luka primer dari film yang mengandung kolagen kulit ikan gabus (Channa striata) dapat menciptakan lingkungan luka dengan kelembapan seimbang (moisture balance) dikenal sebagai metode modern dressing, sehingga akan mempercepat fibrinolisis, mempercepat angiogenesis, menurunkan risiko infeksi, mempercepat pembentukan growth factor, mempercepat pembentukan sel aktif. Kolagen mempunyai peranan penting dalam proses penyembuhan luka karena kolagen mempunyai kemampuan dalam hemostasis, interaksi dengan trombosit, interaksi dengan fibronektin dan meningkatkan eksudasi cairan, meningkatkan komponen selular, meningkatkan faktor pertumbuhan dan mendorong proses fibroplasia. Telah dilakukan pembuatan film untuk balutan primer luka bakar yang mengandung kolagen yang diisolasi dari kulit ikan gabus (Channa striana) 1%, dengan bahan tambahan PVA 4%, propilenglikol 5%, Gliserin 10%, dan pengawet nipagin dan nipasol. Hasil evaluasi gel dan film kolagen diperoleh pH gel 5,44, viskosita gel 1,0378 Poise, tebal film 0,7378 mm, berat jenis film 0,6427 g/cm3 dan water uptake film 47, 41 % (w/w). Dari penelitian yang telah dilakukan dapat disimpulkan hasil evaluasi gel dan film memenuh syarat, tapi perlu penyempurnaan untuk cara pengeringan film, karena film yang mengandung kolagen tidak dapat dikeringkan pada suhu diatas 40oC karena akan merubah kolagen jadi gelatin.

PERBEDAAN KADAR INTERLEUKIN-1β SALIVA ANTARA PENDERITA MYOFASCIAL PAIN DENGAN DISC DISPLACEMENT SENDI TEMPOROMANDIBULA Rafni, Elita; Yanwirasti, Yanwirasti; Darwin, Eriyati; Rikmasari, Rasmi
Dentika: Dental Journal Vol. 19 No. 1 (2016): Dentika Dental Journal
Publisher : TALENTA

Gangguan sendi temporomandibula dapat terjadi karena peradangan neurogenik yang banyak melibatkan sitokin. Pada penderita gangguan sendi temporomandibula dengan keluhan nyeri ditemukan adanya substansi algogenik prostaglandin dan lukotrien sebagai mediator nyeri dan inflamasi. Prostaglandin berasal dari asam arakidonat yang berubah menjadi PGE2 karena aktivitas Cox2 yang diinduksi oleh sitokin IL-1β. Saliva merupakan cairan yang mengandung hormon, antibodi, konstituent mikroba, dan sitokin seperti yang terdapat dalam darah. Penelitian ini dilakukan untuk melihat perbedaan kadar IL-1β pada penderita myofascial pain dan disc displacement sendi temporomandibula. Penelitian ini menggunakan metode cross sectional comparative. Variabel dependen dan independen diperiksa secara bersamaan. Penelitian ini dilakukan di Poliklinik Prostodonsia Rumah Sakit Arifin Ahmad Pekanbaru dan Laboratorium Biomedik Fakultas Kedokteran Universitas Andalas Padang. Pemeriksaan sampel saliva dilakukan pada 37 penderita myofascial pain dan 37 penderita disc displacement. Kadar IL-1β diperiksa dengan Elisa dan data dianalisis dengan t test. Berdasarkan uji statistik Kolmogorov Smirnov ditemukan bahwa data terdistribusi normal baik pada kelompok gender (p =0,772) maupun kelompok umur (p =1,000). Rerata nilai kadar IL-1β penderita myofascial pain adalah 0,22 ± 0,18 nm/L dan penderita disc displacement adalah 0,57 ± 0,45 nm/L. Hasil analisis t-test menunjukkan terdapat perbedaan kadar IL-1β saliva yang signifikan antara penderita myofascial pain dan disc displacement p =0,01 (p<0,05). Sebagai kesimpulan bahwa kadar IL-1β lebih tinggi pada disc displacement dibandingkan myofascial pain.

ANALISIS KONSENTRASI LOW DENSITY LIPOPROTEIN TEROKSIDASI SERUM PADA TAHAPAN ATEROSKLEROSIS Ismawati, Ismawati; Oenzil, Fadil; Yanwirasti, Yanwirasti; Yerizel, Eti
Jurnal Kedokteran Brawijaya Vol 29, No. 4 (2017)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jkb.2017.029.04.11

Aterosklerosis merupakan proses yang terjadi secara bertahap meliputi tahap inisiasi, progresi dan komplikasi. Beberapa penelitian memperlihatkan kaitan antara stres oksidatif dengan aterosklerosis, tetapi belum ada penelitian yang menganalisis perbedaan stres oksidatif pada berbagai tahap aterosklerosis. Konsentrasi oxidized low density lipoprotein (oxLDL) menggambarkan stres oksidatif yang terjadi sehingga dikenal sebagai marker stres oksidatif. Penelitian ini bertujuan untuk menganalisis konsentrasi oxLDL serum pada berbagai tahap aterosklerosis pada hewan coba. Pada penelitian ini dilakukan pengukuran kadar oxLDL serum pada 24 ekor tikus yang terdiri dari kelompok normal, inisiasi, progresi dan komplikasi. Induksi aterosklerosis dilakukan dengan pemberian vitamin D3 (700.000 IU/kg) dan pakan tinggi lipid (kolesterol 2%, lemak kambing 5%, asam kolat 0,2%) masing-masing selama 2 hari, 4 hari dan 2 minggu. Konsentrasi oxLDL serum diukur dengan teknik Enzyme linked immunosorbent assays (ELISA). Data disajikan sebagai rata-rata±SD. Uji statistik yang digunakan adalah uji Anova. Konsentrasi oxLDL serum lebih tinggi pada inisiasi dibandingkan normal (2,17±0,26ng/mL versus 1,93±0,25ng/mL, p>0,05) dan lebih tinggi lagi pada progresi dan komplikasi (2,75±0,25ng/mL dan 3,3±0,61ng/mL, p<0,05) masing-masing terhadap normal dan inisiasi). Penelitian ini menyimpulkan bahwa terdapat perbedaan konsentrasi oxLDL pada berbagai tahap aterosklerosis dan konsentrasi paling tinggi pada tahap komplikasi. Hal ini membuka kemungkinan untuk mengembangkan oxLDL sebagai marker tahapan aterosklerosis.

The Study on Apoptosis of The Liver Cells White Rats Due to the Different Exposure Times and Dosages of Aflatoxin B1 Yanwirasti, Yanwirasti
Majalah Kesehatan Pharmamedika Vol 3, No 1 (2011): JANUARI - JUNI 2011
Publisher : Lembaga Penelitian Universitas YARSI

Apoptosis is known to maintenance of tissue homeostasis and elimination of cancer cells. Whithout programed cell death, cell proliferation would lack on important component of control of oncogenic process. Apoptosis is caused by various inducers such as chemival compound and toxin. Aflatoxin B1 is a potent toxin. In the liver, it undergoes biotransformation which produces reactive oxigen species, causes cellular stress that initiates apoptosis. However, the correlation between exposure of AFB and the evidence of apoptosis in the liver and development of Hepato Cell Carcinoma has not been elucidated.For this purpose, we used an animal experiment with 96 white rats (Rattus Norvegicus). Adult healthy white rats were divided into four groups of 24 rats each, based on the dosages of AFB1 given. Each group was divided further into three subgroups of eight rats based on the length of exposure time to AFB1 .Four dosages of AFB1 , were introduced orally everyday into different groups, consisted of 0, 10, 15 and 20 Ã‚Âµg dissolved in 0,2 ml propylene glycol. Three subgroups received the dosage for 12 weeks, 16 weeks, and 20 weeks. At the end of the experiment, the rats were sacrificed. Liver cells with apoptosis were scrutinized using peroxidase insitu apoptosis detection kit and liver cell damages were examined using histological slices stained by haematoxillin cosin.In our analysis, we found that apoptosis of the liver increased until the formation of dysplacia of the liver cells. After that apoptosis decreased. It means that the highest dosages and the longest time exposure AFB1 inhibited apoptosis.We concluded that apoptosis of the liver cells due to AFB1 caused not only by the damaged of mitochondria (caused by reactive oxygen species) but also by the mutation of p53 which we could see dysplacia of the liver cell in histogical slices.

The Anti-proliferation Effect of an Isolated Butanol Fraction of Tampa Badak (Voacanga foetida (Bl.) K. Schum) Leaves on Leukemia, Lung, and Cervical Cancer Susanty, Adriani; Dachriyanus, Dachriyanus; Yanwirasti, Yanwirasti; Sri Wahyuni, Fatma; Sekar, Andisyah Putri; Alimin, Nur; Magdazaleni, Magdazaleni; Sofia, Sri Esky; Dewi, Citra Kartika
Pharmaceutical Sciences and Research Vol. 7, No. 3
Publisher : UI Scholars Hub

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Voacanga foetida (Bl.) K.Schum leaves are known to have cytotoxic activity against blood cancer cells. This study was aimed to determine the potential of an isolated butanol fraction of Voacanga foetida (Bl.) K.Schum leaves (Tb3 compound) in inhibiting the proliferation of several cancer cells, including leukemia (K562), lung cancer (A549), and cervical cancer (He-La) using the dye exclusion method. We found that the percentage of anti-proliferation of the Tb3 compound was increased in a dose-dependent manner, where it showed 83% (K562), 72.3% (He-La), and 70.7% (A549) inhibition at higher concentration. These values were relatively higher compared to doxorubicin as a positive control, 77.1%, 63.8%, and 62.7%, respectively. Moreover, the IC50 values of the Tb3 compound were still in the range of active cytotoxic compounds based on NCI classification, which is less than 4 µg/mL; 0.5 μg/mL, 2.4 μg/mL, and 3.6 μg/mL for K562, A549, and He-La, respectively. Therefore, this study indicated the potential anticancer effect of an isolated butanol fraction of the Tb3 compound to inhibit cancer cell proliferation, which provides an alternative for cancer therapy.

The Cytotoxic Activity of Marine Sponge-Derived Fungus Aspergillus nomius NC06 Against HT29 Colon Cancer Cells: Cytotoxic Activity of A. nomius NC06 Against HT29 Cancer Cells Artasasta, Muh. Ade; Djamaan, Akmal; Yanwirasti, Yanwirasti; Taher, Muhammad; Djamaludin, Heder; Siswanto, Siswanto; Handayani, Dian
Journal of Tropical Life Science Vol. 14 No. 1 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.01.05

The study of natural products from marine-derived fungi has been interesting tense to researchers as drug discovery sources. Marine fungus from West Sumatera, Indonesia repeatedly showed their potential for cytotoxic and antimicrobial activities. This study aims to determine the cytotoxic activity against HT29 colon cancer cells of each fraction of ethyl acetate extracts from Aspergillus nomius NC06 derived from marine sponge Neopetrosia chaliniformis. A. nomius was cultivated with rice as a growth medium and extracted with ethyl acetate solvent and evaporated in vacuo to obtain ethyl acetate extract. Furthermore, the compounds of ethyl acetate extract were separated with the VLC (Vacuum Liquide Chromatography) method. Five fractions were obtained, which further investigated their cytotoxic activity against HT29 colon cancer cells by using an MTT assay. The result showed that fractions I and III were categorized as potential fractions due to their IC50 value of 13.12 ± 0.39 μg/mL and 2.59 ± 0.19 μg/mL, respectively. It was also supported by ANOVA to measure the effect of each concentration (0.1; 1; 10; 100 μg/mL) of each fraction on the viability percentage of HT29 cells with p < 0.005.

Analisis Gambaran Histopatologis pada Jaringan Kulit Rattus norvegicus yang Mengalami Kekerasan Tumpul Tanpa Jejas Indrayana, Mohammad Tegar; Susanti, Rika; Syahputra, Roni Eka; Hillbertina, Noza; Afandi, Dedi; Yanwirasti, Yanwirasti; Irawati, Nuzulia; Amir, Arni
Jurnal Ilmu Kedokteran Vol 18, No 2 (2024): Jurnal Ilmu Kedokteran
Publisher : Fakultas Kedokteran Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26891/JIK.v18i2.2024.156-161

Blunt trauma without visible skin damage is a common type of violence encountered in physical assault cases. Statistically, at RS Bhayangkara Pekanbaru, 341 cases of blunt trauma without skin damage were reported out of a total of 6,255 assault cases from 2015 to 2020. These cases present a challenge for doctors in finding physical evidence of trauma to document in medicolegal reports (Visum et Repertum). This study aims to analyze the histopathological differences, including erythrocyte extravasation, neutrophil and monocyte cell infiltration, and tissue edema, between groups subjected to blunt trauma without skin damage and a control group. Using a post-test-only group design, this study involved 30 Rattus norvegicus samples divided into three groups: control, intervention group 1, and intervention group 2. The intervention involved a 324-gram load dropped from a height of 45 cm onto the surface of the rats' skin. Histopathological examination revealed no significant differences in erythrocyte extravasation, neutrophil infiltration, or monocyte infiltration, while a significant difference was observed in tissue edema levels.

Malondialdehyde Expression in HT29 Cells with Blastocystis sp. Exposure Rusjdi, Selfi Renita; Irawati, Nuzulia; Yanwirasti, Yanwirasti; Hilbertina, Noza
Journal of Agromedicine and Medical Sciences Vol. 10 No. 3 (2024)
Publisher : Faculty of Medicine, Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v10i3.47044

Blastocystis sp. is one of the intestinal protozoa that is frequently found in stool specimens of colorectal cancer patients. This protozoan infection will trigger the host cell defense response to produce Reactive Oxygen Species (ROS) which causes oxidative stress. Oxidative stress can cause and aggravate colorectal cancer. Lipid peroxidation is one of the best indicators of ROS levels. Malondialdhyde is its end product so it is used as a marker of oxidative stress. This study aims to determine the exposure of Blastocystis sp. on malondialdehyde expression in colorectal cancer cell line HT29. This research is an experimental study in vitro. HT29 cells were treated with various concentrations of Blastocystis sp. antigen (0.005 Î¼g/ml, 0.01 Î¼g/ml, 0.05 Î¼g/ml, 0.1 Î¼g/ml and 0.5 Î¼g/ml) for 72 hours. Malondialdehyde expression was examined using spectrophotometric method. Data analysis was performed using One Way Anova test. The results of the study are the mean expression of malondialdehyde in nmol/ml. The mean malondialdehyde levels of the control group, treatment with concentrations of 0.005 Î¼g/ml, 0.01 Î¼g/ml, 0.05 Î¼g/ml, 0.1 Î¼g/ml and 0.5 Î¼g/ml were 3.134 nmol/ml, 2.682 nmol/ml, 2.556 nmol/ml, 2.744 nmol/ml, 2.84 nmol/ml and 3.286 nmol/ml respectively. The conclusion is that there is an effect of exposure to Blastocystis sp. on malondialdehyde expression of HT29 cells. Keywords: Blastocystis sp., HT29, malondialdehyde

Pengaruh Likopen terhadap Kadar Soluble FMS-Like Tyrosine Kinase 1 pada Preeklamsi Basyir, Vaulinne; Fauziah, Prima Nanda; Pramatirta, Akhmad Yogi; Yanwirasti, Yanwirasti; Mose, Johanes C.; Oenzil, Fadil
Andalas Journal of Health Vol. 9 No. 4 (2020): Online December 2020
Publisher : Faculty of Medicine, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jka.v9i4.1513

Preeklamsi merupakan penyebab utama morbiditas dan mortalitas baik maternal maupun perinatal. Tujuan: Menganalisis pengaruh pemberian likopen terhadap kadar sFlt-1 secara in vitro pada sel trofoblas model preeklamsi. Metode: Penelitian ini dilakukan secara eksperimental di Laboratorium Genetika Molekuler dan Kultur Sel Fakultas Kedokteran Universitas Padjadjaran selama periode 2017-2018. Sampel serum diperoleh dari RS. Dr. Hasan Sadikin Bandung, sesuai kriteria inklusi dan eksklusi. Kriteria inklusi adalah pasien yang telah didiagnosis preeklamsi berdasarkan pemeriksaan tekanan darah dan protein urin, dan kriteria eksklusi adalag pasien yang tidak datang kontrol atau tidak bisa dihubungi. Penelitian ini terdiri dari dua tahap, pertama ditentukan nilai LD50 likopen terhadap sel trofoblas kemudian dijadikan dasar nilai konsentrasi yang akan digunakan, selanjutnya dilakukan pemeriksaan sFlt-1 menggunakan metode Elisa pada kultur sel trofoblas kondisi normal dan preeklamsi dengan analisa data menggunakan analisis varians (Anova) dan uji Duncan untuk mengetahui perbedaan antar variabel. Hasil: Nilai LD50 adalah 250 ug/ml dan serial konsentrasi yang digunakan mulai dari 1,953 sampai dengan 125 ug/ml. Didapatkan kadar sFlt-1 menurun secara signifikan (p0,05) dari 39,5445 menjadi 31,1342 pg/ml pada sel trofoblas yang diinduksi dengan serum preeklamsi setelah pemberian konsentrasi likopen sebesar 15,625-31,25 ug/ml yang nilainya mendekati keadaan pada sel trofoblas yang diinduksi serum kehamilan normal. Pemberian likopen dapat menurunkan kadar sFlt-1 pada sel trofoblas preeklamsi. Simpulan: Likopen dapat merubah kadar sFlt-1 dan perubahannya dipengaruhi oleh konsentrasi dan jenis serum.Kata kunci: likopen, preeklampsi, sFlt-1Â

The Cytotoxic Activity of Marine Sponge-Derived Fungus Aspergillus nomius NC06 Against HT29 Colon Cancer Cells: Cytotoxic Activity of A. nomius NC06 Against HT29 Cancer Cells Artasasta, Muh. Ade; Djamaan, Akmal; Yanwirasti, Yanwirasti; Taher, Muhammad; Djamaludin, Heder; Siswanto, Siswanto; Handayani, Dian
Journal of Tropical Life Science Vol. 14 No. 1 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.01.05

The study of natural products from marine-derived fungi has been interesting tense to researchers as drug discovery sources. Marine fungus from West Sumatera, Indonesia repeatedly showed their potential for cytotoxic and antimicrobial activities. This study aims to determine the cytotoxic activity against HT29 colon cancer cells of each fraction of ethyl acetate extracts from Aspergillus nomius NC06 derived from marine sponge Neopetrosia chaliniformis. A. nomius was cultivated with rice as a growth medium and extracted with ethyl acetate solvent and evaporated in vacuo to obtain ethyl acetate extract. Furthermore, the compounds of ethyl acetate extract were separated with the VLC (Vacuum Liquide Chromatography) method. Five fractions were obtained, which further investigated their cytotoxic activity against HT29 colon cancer cells by using an MTT assay. The result showed that fractions I and III were categorized as potential fractions due to their IC50 value of 13.12 ± 0.39 μg/mL and 2.59 ± 0.19 μg/mL, respectively. It was also supported by ANOVA to measure the effect of each concentration (0.1; 1; 10; 100 μg/mL) of each fraction on the viability percentage of HT29 cells with p < 0.005.

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