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Identification and Role of Microalgae Found in Catfish (Clarias gariepinus) Cultivation Pond Inayah, Zakiyyah Nur; Kurniawan, Rahmat Ardi; Aulia, Muhamad Fajar; Pratama, Adif; Islamy, R Adharyan
Nekton Vol 5 No 2 (2025): Nekton
Publisher : Politeknik Negeri Sambas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47767/nekton.v5i2.1096

Abstract

Microalgae play an essential role in aquaculture as primary producers, serving both as natural feed and as bioindicators for assessing pond water quality. The presence of specific microalgal taxa is crucial for evaluating aquatic conditions and identifying potential natural feed sources. This study aimed to identify microalgal species found in African catfish (Clarias gariepinus) culture ponds and to determine their ecological roles. A descriptive survey method was applied. Water samples were collected using a plankton net from three catfish culture ponds during August–September 2025. Identified microalgae were morphologically classified and analyzed for their potential as bioindicators based on literature review using Palmer’s Pollution Index. The results revealed 16 genera belonging to three divisions: Bacillariophyta (diatoms), Chlorophyta (green algae), and Cyanophyta (blue-green algae). Two genera of Bacillariophyta were identified—Aulacoseira and Synedra. Chlorophyta was the most dominant division, comprising 11 genera: Actinastrum, Coelastrum, Dictyosphaerium, Golenkinia, Micractinium, Oocystis, Pandorina, Pediastrum, Scenedesmus, Staurastrum, and Ulothrix. Cyanophyta included three genera—Anabaena, Aphanocapsa, and Spirulina. Among these, Scenedesmus and Pediastrum (Chlorophyta) are commonly used as natural feed, whereas Cyanophyta members are often utilized as water-quality bioindicators. The diversity and abundance of microalgae observed in the catfish ponds indicate organic enrichment, yet they also provide valuable potential as natural feed sources.
Evaluation of Nutritional Composition and Lead Detection in Suckermouth Catfish (Pterygoplichthys sp.) Egg Flour as a Candidate Feed Additive for Fish Feed Masriah, Andi; Dwi Cahya, Muhamad; Islamy, R Adharyan; Nur Nadiro, Vina; Maulidiyah, Vika; Supriyadi, Supriyadi; Ramadhan, Adam Ashil; Hardiyanto, Revanina Putri
Torani Journal of Fisheries and Marine Science Vol. 9 No. 1 (2025): VOLUME 9, NOMOR 1, DECEMBER 2025
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35911/torani.v9i1.46360

Abstract

This study aims to evaluate the nutritional value and heavy metal lead (Pb) content in suckermouth catfish (Pterygoplichthys sp.) egg meal as a candidate feed additive in fish feed. The fish eggs were obtained from fishermen's catches in public waters in East Java, then cleaned, dried, and processed into meal for analysis. The proximate parameters tested included protein, fat, crude fiber, and ash content using the AOAC standard method, while Pb content was analyzed using Atomic Absorption Spectrophotometry (AAS). The results showed that suckermouth catfish egg meal had the following nutritional composition: protein 39.25±0.02%, fat 9.19±0.01%, crude fiber 2.36±0.03%, and ash 4.95±0.02%. This nutritional profile indicates that suckermouth catfish eggs are a high source of animal protein and have a low fiber content, thus having the potential to improve feed quality through the addition of essential amino acids and energy. Heavy metal Pb content was not detected within the sensitivity limits of the instrument, thus this product meets the safety aspects of feed ingredients related to heavy metal contamination. Overall, the results of this study indicate that suckermouth catfish egg meal has competitive nutritional value compared to several conventional feed ingredients and is safe from Pb contamination. Thus, this ingredient has the potential to be developed as an alternative feed additive in fish feed formulations.
Assessing the Genotoxic Potentials of Methomyl-based Pesticide in Tilapia (Oreochromis niloticus) Using Micronucleus Assay Islamy, R Adharyan; Yanuhar, Uun; Hertika, Asus Maizar Suryanto
The Journal of Experimental Life Science Vol. 7 No. 2 (2017)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1172.773 KB) | DOI: 10.21776/ub.jels.2017.007.02.05

Abstract

Pesticides are recognized as serious pollutants in the aquatic environment with the potential to cause genotoxic on the aquatic organism, especially fish. The micronucleus (MN) assay has been used to evaluate genotoxicity of many compounds in polluted ecosystems such pesticides. The aim of this study to determine genotoxic effect of methomyl-based pesticide on tilapia (Oreochromis niloticus). Fish were exposed to six different concentrations base on range finding test (0 ppm, 3.2 ppm, 4.2 ppm, 6.5 ppm, 8.7 ppm and 10 ppm) of methomyl-based pesticide. The micronucleus were collected from peripheral blood erythrocyte of fish after 96 h exposure. Peripheral blood samples smears were stained with Giemsa, MN frequencies were counted and statistically analyzed using one-way ANOVA. The result of this study showed after 96 hours exposed to methomyl-based pesticide, at concentration 0 ppm causes 0% mortality, at concentration to 3.2 ppm causes 30% mortality, at concentration 4.2 ppm causes 60% mortality, at concentration 6.5 ppm causes 70% mortality, at concentration 8.7 ppm causes 80% mortality, at concentration 10 ppm causes 100% mortality of fish test. Lethal Concentration 50 (LC50 - 96 hours) of methomy-base pesticide towards tilapia (O. niloticus) is 4.015 ppm. Through micronuclei assay during 96 hour exposure of methomyl-based pesticide, the result shows that frequencies of micronuclei in erythrocyte of fish test at concentration at 0ppm is 12‰, 18‰ and 16‰; at concentration at 3.2ppm is 33‰, 26‰ and 29‰; at concentration at 4.2ppm is 41‰, 38‰ and 46‰; at concentration at 6.5ppm is 68‰, 81‰ and 82‰; at concentration 8.7ppm is 133‰, 130‰ and 137‰; at concentration 10ppm is 163‰, 166‰ and 156‰. It revealed that methomyl-based pesticide exposure induced after 96 h significantly (P<0.05) increased genotoxic potentials simultaneous with increased concentration.Keywords: Genotoxic, Methomyl, Micronucleus Assay, Pesticide, Tilapia.