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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Agrivet AL-HAYAT: Journal of Biology and Applied Biology Jurnal Riset Biologi dan Aplikasinya Berkala Ilmiah Biologi Buitenzorg: Journal of Tropical Science
Damayanti, Frisca
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Economics, Econometrics & Finance Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Veterinary

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Journal : Berkala Ilmiah Biologi

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Cytotoxic Activity of Castanopsis argentea (Blume) A.DC. Extract and Fraction on MCF-7 Breast Cancer Cell Nuringtyas, Tri Rini; Nastiti, Anisa Diana; Surta, Muhammad Imam; Lailaty, Intani Quarta; Damayanti, Frisca
Berkala Ilmiah Biologi Vol 16 No 1 (2025)
Publisher : Fakultas Biologi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/bib.v16i1.9851

Abstract

Marine algae is a biological source that is rich in bioactive activity. Marine algae is an important source of macronutrients, especially proteins and lipids, as well as micronutrients such as vitamins and minerals along with dietary fiber and other minoritarian constituents such as polyphenols. This study aimed to determine the antioxidant of hydrolysate protein of Sargassum sp. as. The work method was started by collecting Sargassum sp. in the intertidal zone of the South Coast of Gunungkidul, Yogyakarta and then brought to the laboratory for extraction. Protein hydrolysate was obtained by protein extraction using phosphate buffer and triRNA kit. Several buffers was used to optimize the extraction, namely PBS buffer, Tris HCl buffer and plant extraction buffer The protein obtained were evaluated in the SDS-PAGE electrophoresis. The isolated protein subjected for the trypsin digestion to obtain the hydrolysate protein then followed by antioxidant assays using DPPH test. Data analysis was carried out using ANOVA statistics for the antioxidant test. Several ratios of trypsin and protein are used to find the optimal ratio to produce protein hydrolysate. From the result it was observed that the best buffer solvent to use was Tris HCl buffer for the isolation of Sargassum sp. This can affect the protein profile of the Sargassum sp protein hydrolysate. by producing higher protein precipitates than other buffer types.
Antibacterial Activity of Leaf and Twig Extracts of Castanopsis argentea (Blume) A.DC. Against Escherichia coli and Staphylococcus aureus Amalia, Alifa; Lailaty, Intani Quarta; Surya, Muhammad Imam; Nuringtyas, Tri Rini; Damayanti, Frisca
Berkala Ilmiah Biologi Vol 16 No 1 (2025)
Publisher : Fakultas Biologi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/bib.v16i1.19731

Abstract

Castanopsis argentea is a potential souce of natural antibiotics that has not been studied much. The purpose of this study was to analyze the antibacterial activity and study the content of secondary metabolites from leaves and twigs extract of Castanopsis argentea against Escherichia coli and Staphylococcus aureus bacteria. This study used the paper disk method to see antibacterial activity. Antibacterial activiy was indicated by the formation of a clear zone around the dic paper. Samples were made into three treatments, namely the concentration of 20 mg/ml; 40 mg/ml; and 80 mg/ml. the solvent used was selected based on the level of polarity namely ethyl acetate, 70% ethanol, and aquades. The results of the antibacterial activity test showed that the extract with the most potential to inhibit bacterial growth was ethyl acetate twig extract at a concentration of 80 mg/ml with an average diameter of 6mm for Escherichia coli bacteria and 8.67 mm for Staphylococcus aureus bacteria. The compounds identified in the ethyl acetate twig extract are terpenoids, phenolics, and flavonoids. The results of profiling using UV-Vis spectrophotometry showed that the maximum absorption of terpenoids, flavonoids, and phenolics compounds was found in the wavelength range of 300-600 nm. The secondary metabolite compounds contained in the leaf and twig extracts of Castanopsi argentea make this species a potential natural antibacterial.
Co-Authors , Triadiati Albertus Husein Wawo Amalia, Alifa Danang Wahyu Purnomo Dhavalyra, Salwa DIAN LATIFAH, DIAN Endewip, Lidya N. Fitri Fatma Wardani Hamim Hamim Harto, Harto Helmanto, Hendra Husaini, Iin P.A Hutabarat, Prima Wahyu K. Hutabarat, Prima Wahyu Kusuma Indra Gunawan Indresputra, Faozan Iyan Robiansyah Kholidah, Nur Kusumaningtyas Pitaloka, Mutiara Lailaty, Intani Quarta MARTIANSYAH, Irfan Mayta Novaliza Isda Mochammad Imron Awalludin Muhammad Imam Surya Muhammad Rifqi Hariri Mujahidin Mujahidin Mulyani, Melza Nastiti, Anisa Diana Ning Tyas, Kartika Ninik Setyowati Peni Lestari Pratiwi, Widya Ayu Primananda, Enggal Putri Maulida, Azkia RACHMADIYANTO, ARIEF NOOR Rahmaningtiyas, Lutfi Rinandio, Dipta Sumeru Rismona, Nurwani Safarinanugraha, Dina Setyanti, Dwi SRI RAHAYU Suhatman, Agus Surta, Muhammad Imam Tri Rini Nuringtyas Yudaputra, Angga zulkarnaen, rizmoon Zulkarnaen, Rizmoon Nurul