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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Kedokteran Brawijaya
Nugraha, Rivo Yudhinata Brian
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Agriculture, Biological Sciences & Forestry Environmental Science Medicine & Pharmacology

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The Use of Natural Phenomenon in Obtaining Pregnant Rats and Mice as Experimental Animals with The Same Gestational Ages Sardjono, Teguh Wahju; Gondo, Harry Kurniawan; Nugraha, Rivo Yudhinata Brian; Putri, Anisa Tiara; Effendy, Meyland Citra Oktri Sienty
Journal of Tropical Life Science Vol 9, No 3 (2019)
Publisher : Journal of Tropical Life Science

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Abstract

Hormonal oestrus synchronization is often used to obtain the same gestational ages animal models, but it may not physiologic nor practical. This research was conducted to find out a simple method in obtaining physiologic pregnant experimental animals. We used 120 female rats and mice each, divided into 16 groups with 15 members each. They were classified according to the state of parities, pair or group mating, and synchronization of oestrous. The estrous synchronization is done by utilizing the natural phenomenon (Lee-Boot, Pheromone, and Whitten effect). All nulliparous and multiparous models then were simultaneously mated for one night in pairs (1:1) or in groups (1:5) subsequently. Pregnancy rates were noted based on the number of models delivering pups on day 19-23 post-mating. The highest pregnancy rate was noted in group of multiparous, synchronized and monogamous rats (86.7%), followed by nulliparous, synchronized and monogamous rats (73.3%) and multiparous, synchronized and monogamous mice (60%) (p<0.05). Synchronization of the estrous and monogamous mating of rats or mice had significant roles in pregnancy rates (p<0.05). To get a high number of pregnant rats or mice models, we suggest to use the multiparous, estrous synchronized and monogamous simultaneous mating within one night.
Growth Rate Differences of Chrysomya sp. Larvae on Rattus novergicus Wistar Strain Corpse Exposed and Unexposed to Ephedrine Toxic Dose Poeranto, Sri; Prastowo, Wening; Resmi, Diandra Laksmita; Nugraha, Rivo Yudhinata Brian
Journal of Tropical Life Science Vol 7, No 3 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.03.06

Abstract

Post Mortem Interval (PMI) is used as a parameter to define the onset, cause, manner, and time of death to help maintenance of law and identify crime victims by the age of the larvae found. Larval growth is influenced by the temperature, humidity, and contaminant (drug or toxic). This experiment used two media of dead rats (200 grams), one given ephedrine of LD50 = 266 mg/kg and the other without ephedrine. Both were put in 2 cages each containing 50 Chrysomya sp. Ten larvae were randomly taken every day for 14 days to be measured their length, weight, and duration of growth. The 3rd larvae stage in the media with ephedrine had weight gain on 5th – 6th day morning, while larvae in media without ephedrine had static weight gain on 5th day morning - afternoon and weight decrement on 6 – 7th day morning. Larva’s peak ratio of length/weight in the media with ephedrine was higher than that of larvae in the media without ephedrine. Larvae on media with ephedrine grew faster in 1st, 2nd, 3rd larvae stage, and pupal stage compared to larvae without ephedrine. Therefore, this study indicates that ephedrine can accelerate the growth rate of Chrysomya sp. larvae.
Co-Authors Ariel, Dio Giovanni Cahyono, Alfian Wika Effendy, Meyland Citra Oktri Sienty Erwan, Nabila Erina Harry Kurniawan Gondo Loeki Enggar Fitri Mardhiyyah, Kana Putri, Ajeng Maharani Putri, Anisa Tiara Putri, Fitria Febriliani Resmi, Diandra Laksmita Sri Poeranto, Sri Sri Winarsih Teguh Wahju Sardjono Wening Prastowo