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All Journal Animal Production : Indonesian Journal of Animal Production Jurnal Medik Veteriner
Dikman, Dicky Mohammad
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Veterinary

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Calves Productivity with Applying Integrated Village Management System (IVMS) and Rice-Straw Based Feed Ratnawati, Dian; Dikman, Dicky Mohammad; Affandhy, Lukman
ANIMAL PRODUCTION Vol 21, No 1 (2019)
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (388.983 KB) | DOI: 10.20884/1.jap.2019.21.1.627

Abstract

The objective of the study was to assess the productivity of PO calves with Integrated Village Management System (IVMS). The calf management generally follows cow pattern.  The IVMS management applied to calves include weaning 5-6 months and optimizing local feed. Straw-based cow feed with supplementation using greenfeed and concentrate. Recording productivity of calf include: birth, weaning, body weight, calf death calf mobilization. Weighing weight was done at birth, weaning, and every month in the first year of study and every 2 months in the next research year. Implementation of IVMS management with rice straw-based feed produces good PO calf productivity when supported by addition of other feed sources, i. e. feed with high protein composition (concentrate, legume) to balancing the nutrient composition of feed.
Detection of Anti-Müllerian Hormone (AMH) mRNA in Serum and Ovarian Tissue of Local Indonesian Cattle Using EvaGreen-based RT–qPCR Dikman, Dicky Mohammad; Puspitasari, Heni; Waluyo, Seagames; Kurniawan, Muhammad ‘Ahdi; Supriyadi, Supriyadi; Srianto, Pudji; Luqman, Epy Muhammad; Suprayogi, Tri Wahyu; Madyawati, Sri Pantja; Safitri, Erma; Lestari, Tita Damayanti
Jurnal Medik Veteriner Vol. 8 No. 2 (2025): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss2.2025.382-397

Abstract

Anti-Müllerian hormone (AMH) has been identified as a potential biomarker for assessing ovarian reserve and reproductive capacity in cattle, with high heritability and relatively stable expression. However, studies detecting AMH mRNA in bovine serum remain scarce due to the matrix’s inherently low RNA yield, susceptibility to degradation, and potential presence of PCR inhibitors. This study provides an exploratory validation of AMH mRNA detection in serum — a challenging matrix compared with ovarian tissue — using EvaGreen-based reverse transcriptase–quantitative PCR (RT–qPCR). Specific primers for the AMH and β-actin genes were designed in silico and validated through melting curve analysis and linearity testing. The results showed high amplification efficiency (AMH: 100.2%, R² = 0.994; β-actin: 109.1%, R² = 0.996), with specific amplification of both targets. AMH detection in serum samples was successful in some samples, while the β-actin gene was consistently amplified as a reference gene. Despite the low RNA quality from serum and the presence of organic contaminants, the method demonstrated its feasibility for detecting AMH transcripts in a minimally invasive manner. Physiologically, AMH levels positively correlate with antral follicle count, superovulation success, and embryo quality, and are sensitive to heat stress and other environmental factors. These findings provide a foundational basis for developing molecular diagnostic approaches based on AMH gene expression in cattle reproductive management programs and support the future development of efficient, accurate, and context-specific biomolecular-assisted selection technologies for tropical livestock systems.
Co-Authors Dian Ratnawati Erma Safitri Heni Puspitasari Kurniawan, Muhammad ‘Ahdi Lukman Affandhy Luqman, Epy Muhammad Pudji Srianto Seagames Waluyo, Seagames Sri Pantja Madyawati, Sri Pantja Supriyadi Supriyadi Tita Damayanti Lestari Tri Wahyu Suprayogi