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All Journal Journal Of Vocational Health Studies Jurnal Medik Veteriner Veterinary Biomedical and Clinical Journal Darmabakti Cendekia: Journal of Community Service and Engagements journal of Basic Medical Veterinary Jurnal Pengabdian Masyarakat Charitas Central Community Development Journal Jurnal Kesehatan Masyarakat
Sri Pantja Madyawati, Sri Pantja
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Parasites Prevalence of Dairy Cattle in Argopuro Area, Krucil District, Probolinggo Regency, Indonesia Pradana, Giffari Danindra; Mufasirin; Madyawati, Sri Pantja; Tri Suwanti, Lucia; Kusnoto; Sunarso, Agus; Aryaloka, Suhita
Veterinary Biomedical and Clinical Journal Vol. 5 No. 2 (2023)
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.VetBioClinJ.2023.005.02.3

Abstract

This study aimed to identify endoparasitic species and their prevalence in dairy cows in the Probolinggo, Indonesia. This survey was conducted in the Cooperation of Argopuro, in the hill side of Krucil district, Probolinggo regency, during rainy season from March until July 2020. Faecal samples were collected (n=100), and three fecal examinations were performed for parasite identification: native, sedimentation, and flotation techniques. Results showed that the prevalence of endoparasitosis was 56%; 29% was due to helminthiases and the other 37% was of Balantidium coli. Fasciola sp., Oesophagostomum sp., Gaigeria pachyscelis, Toxocara vitulorum, Mecistocirrus digitatus, Chabertia sp. were among the helminths detected. The Lucient Brump test indicated that among samples infected with helminths (n=29), 89.7% were mild, 6.9% were moderate and 3.4% were severely infected. Further, the study estimated that the level of burden with Balantidium coli was identified to be mild in 62.2%, moderate in 32.4%, and severe in 5.4% of the positive samples, respectively (n=37). This study indicates that during the commencement of the rainy season, the campaign of effective endoparasitic control could be advisable in the study area
Persentase Motilitas dan Viabilitas Spermatozoa Kambing Sapera dalam Pengencer Tris Kuning Telur dan Susu Skim Kuning Telur Before Freezing Masyitoh, Herlina; Suprayogi, Tri Wahyu; Praja, Ratih Novita; Srianto, Pudji; Madyawati, Sri Pantja; Saputro, Amung Logam
Jurnal Medik Veteriner Vol. 1 No. 3 (2018): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (417.557 KB) | DOI: 10.20473/jmv.vol1.iss3.2018.105-112

Abstract

Penelitian ini bertujuan untuk mengetahui perbedaan persentase motilitas dan viabilitas semen kambing Sapera terhadap evaluasi semen segar dan before freezing dengan penambahan pengencer tris kuning telur dan susu skim kuning telur. Metode penelitian ini adalah pengambilan semen, evaluasi semen segar (pemeriksaan makroskopis dan mikroskopis), pengenceran semen, gliserolisasi, ekuilibrasi dan evaluasi before freezing. Penelitian ini terdiri dari P0, P1 dan P2, masing-masing perlakuan terdiri dari sembilan ulangan. P0 adalah semen segar kambing Sapera, P1 adalah semen kambing Sapera+pengencer tris kuning telur dan P2 adalah semen kambing Sapera+pengencer susu skim kuning telur. Data yang diperoleh dengan Uji T2 sampel dependent, membandingkan P0 dan P1 serta P0 dan P2. Data menunjukkan perbedaan sangat nyata (p<0,01) pada perlakuan P0 dan P1 persentase motilitas 76,11%; 65,56% dan viabilitas 82,33%;72,22%. P0 dan P2 persentase motilitas 76,11%;61,11% dan viabilitas 82,33%;67,78%. Hasil tersebut menunjukkan bahwa penelitian persentase motilitas dan viabilitas spermatozoa kambing Sapera mengalami penurunan dari semen segar pada tris kuning telur dan susu skim kuning telur before freezing.
Efektifitas Daya Pisah Electric Separating Sperm (ESS) terhadap Spermatozoa Kromosom X dan Y pada Kambing Sapera Lailiyah, Fahlevi; Srianto, Pudji; Saputro, Amung Logam; Madyawati, Sri Pantja; Agustono, Bodhi; Prastiya, Ragil Angga
Jurnal Medik Veteriner Vol. 1 No. 3 (2018): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (399.223 KB) | DOI: 10.20473/jmv.vol1.iss3.2018.93-98

Abstract

Penelitian ini bertujuan untuk mengetahui efektifitas pemisahan alat Electric Separating Sperm (ESS) terhadap spermatozoa kromosom X dan Y pada sisi anoda dan katoda dengan variabel meliputi presentase viabilitas, motilitas, abnormalitas dan morfometri spermatozoa. Metode pengambilan semen menggunakan vagina buatan, kemudian dilakukan pemeriksaan makroskopis dan mikroskopis, apabila menunjukkan hasil yang baik dilanjutkan dengan pengenceran semen dengan Tris Kuning Telur dan dilakukan pemisahan. Terdapat tiga perlakuan pada penelitian ini yaitu P1 (3 menit), P2 (7 menit), P3 (10 menit) dengan enam kali pengulangan pada sisi anoda dan katoda. Data pada tiap variabel (viabilitas, motilitas dan abnormalitas) diperiksa menggunakan analisis statistik ANOVA dan untuk melihat adanya pemisahan spermatozoa kromosom X dan Y dilakukan pengukuran terhadap kepala menggunakan morfometri. Hasil pemisahan tertinggi yaitu pada P3 dengan perbandingan pemisahan X : Y sejumlah 65,00:35,00 pada sisi katoda, kemudian pada sisi anoda persentase perbandingan X : Y yaitu 59,00:41,00. Hasil kualitas spermatozoa setelah pemisahahn menunjukkan presentase viabilitas terbaik terdapat pada P1 yaitu 75,00a±2,00 pada sisi anoda dan 76,50a±2,17 pada sisi katoda. Motilitas spermatozoa P1 menunjukkan motilitas progresif spermatozoa dengan rataan 60,83a±2,04 pada anoda dan 61,67a±2,58 pada sisi katoda. Presentase abnormalitas tidak menunjukkan perbedaan yang signifikan antar perlakuan.
Produksi dan Kualitas Telur Itik Alabio di Daerah Sentra Peternakan Desa Sungai Pandan, Kabupaten Hulu Sungai Utara, Kalimantan Selatan Fajarwati, Rini; Sarmanu, Sarmanu; Nidom, Chairul Anwar; Madyawati, Sri Pantja; Mustofa, Imam; Lamid, Mirni; Hidanah, Sri; Lokapirnasari, Widya Paramita; Purnomo, Tarzan; Sukmanadi, Mohammad
Jurnal Medik Veteriner Vol. 3 No. 2 (2020): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol3.iss2.2020.246-250

Abstract

Penelitian ini merupakan penelitian deskriptif dengan menggunakan 20 itik Alabio betina siap bertelur dengan rentang usia 6 bulan dan untuk pemeriksaan kualitas telur untuk masing-masing kelompok 10 sampel. Pengamatan tingkat produksi telur dilakukan selama tiga bulan dengan 10 pengulangan, kualitas telur (ketebalan kulit telur, persentase kulit telur, persentase kulit telur, persentase albumin, persentase kuning telur, huagh unit, warna kuning telur, kadar protein dan lemak kandungan). Data produksi telur dan kualitas telur diuji menggunakan uji Anova. Hasil pengamatan rata-rata produksi harian telur 15.45 ± 0.12, 14.72 ± 0.10, 14.91 ± 0.09; produksi berat harian (kg/hari) 925.27 ± 7.50, 875.40 ± 6.32, 884 ± 5.03; hasil dari ketebalan kulit telur kualitas 0.33 ± 0.01, 0.33 ± 0.12, 0.33 ± 0.12; persentase kulit telur 11.32 ± 0.93, 11.28 ± 0.10, 11.36 ± 0.05; persentase albumin 55.70 ± 1.08, 56.66 ± 1.22, 56.12 ± 1.00; persen kuning telur 32.07 ± 1.24, 31.55 ± 1.48, 31.64 ± 1.20; Haugh unit 88.70 ± 6.21, 91.41 ± 6.70, 94.51 ± 5.06. Warna kuning masing-masing memiliki skor 15. Dapat disimpulkan bahwa produksi dan kualitas telur itik Alabio baik.
Perbandingan Pengencer Tris Kuning Telur dan Susu Skim Kuning Telur Terhadap Persentase Motilitas, Viabilitas dan Integritas Membran Plasma Spermatozoa Kambing Sapera pada Penyimpanan Suhu 5ºC Iskandari, Novia Nur; Madyawati, Sri Pantja; Wibawati, Prima Ayu; Suprayogi, Tri Wahyu; Prastiya, Ragil Angga; Agustono, Bodhi
Jurnal Medik Veteriner Vol. 3 No. 2 (2020): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol3.iss2.2020.196-202

Abstract

Penelitian ini bertujuan untuk mengetahui perbedaan pengencer tris kuning telur dan susu skim kuning telur terhadap persentase motilitas, viabilitas dan integritas membran plasma spermatozoa kambing Sapera. Metode penelitian dengan mengumpulkan semen, pemeriksaan kelayakan semen segar, pengenceran semen, penyimpanan suhu 5ËšC dan pemeriksaan setiap 24 jam. Data diolah menggunakan anova faktorial dengan membandingkan antara dua bahan pengencer terhadap waktu pemeriksaan. Data menunjukkan tris kuning telur signifikan (p<0.05) terhadap susu skim kuning telur. Lama penyimpanan menunjukkan hasil signifikan pada hari ke-1 aspek motilitas 84.44% : 80%, viabilitas 89.67% : 86% dan integritas membran plasma 83.67% : 79.33%. Tris kuning telur dan susu skim kuning telur menunjukkan adanya interaksi (p<0.05 ) terhadap waktu pemeriksaan motilitas namun tidak terdapat interaksi (p>0.05) terhadap waktu pemeriksaan viabilitas dan integritas membran plasma. Disimpulkan pengencer tris kuning telur dan susu skim kuning telur berpengaruh pada persentase motilitas, viabilitas dan integritas membran plasma spermatozoa.
The Steroid Hormone Profile in Etawah Crossbreed Goat While Ovulation Induced using The Selectsynch Method Aftabuddin Rz, Muhammad; Srianto, Pudji; Nidom, Chairul Anwar; Madyawati, Sri Pantja; Sardjito, Trilas; Ismudiono, Ismudiono
Jurnal Medik Veteriner Vol. 5 No. 1 (2022): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol5.iss1.2022.62-68

Abstract

This study aimed to provide alternative information and solutions in an effort to increase reproductive productivity in etawah crossbreed goats (PE). The sample used in this study was 10 female PE with an average age of 2.5 - 3 years and primiparous at least . Ovulation induction was performed using 0.1 mg intra-muscular Gonadotropin-Releasing Hormone (GnRH), after seven days injection of Prostaglandin F2α (PGF2α) was given as much as 2.5 mg submucosa of the vulva, followed by a second injection of GnRH as much as 0,2 mg intramuscular in samples that have really experienced heat and selected selectively according to the signs of natural heat shown by the sample and followed by insemination as much as 2 doses or 0.50 ml of frozen semen. Blood sampling was performed at H0, H7th, H14th and H21th after insemination. All blood samples were collected and progesterone and estrogen hormone profiles were examined using the ELISA method. From the results of the ELISA test, the mean progesterone hormone profile H0 = 4.798 ng / ml, H7th = 4.887 ng / ml, H14th = 4.824 ng / ml, H21th = 5.148 ng / ml. The profile of the hormone estrogen at H0 = 19,461 pg / ml, H7th = 17,457 pg / ml, H14th = 18,248 pg / ml, H21th = 17,515 pg / ml. This study showed an increase in the levels of the progesterone hormone at H0 to H7th, then slightly decreased in H14th and a significant increase in H21th. For the estrogen hormone, there is a decrease from H0 to H7th then there is a slight increase in H14th and decreases again in H21th.
Detection Gene of Luteinizing Hormone Receptor (rLH) in Madrasin Cattle using Polymerase Chain Reaction Prasetyo, Ivo Febrina; Utomo, Budi; Madyawati, Sri Pantja
Jurnal Medik Veteriner Vol. 4 No. 1 (2021): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss1.2021.125-130

Abstract

The aim of this study was to determine the profile of the Luteinizing Hormone Receptor (rLH) gene in Madrasin cross breed cattle. The sample of this study used blood samples from 3 breeds of cattle. There were Madrasin from Bangkalan City, Madura from Bangkalan City and Limousin from BET Cipelang Bogor. The sample criteria were used female cattle around 2,5 years old and ever partus (once). The genomic DNA was extracted for polymerase chain reaction (PCR), followed by sequencing with software BioEdit Vers. 8.0 to determine the sequence of nucleotide bases. The results of this study were that three samples detected fragments in the form of DNA with nucleotide base of 373 bp. The conclusion, gene rLH in Madrasin Cattle can detected using PCR.
Efficacy of Grass and Concentrate Ratio on Milk Urea Nitrogen, Progesterone, and Pregnancy Status in Friesian Holstein Hardaningtyas, Enggar; Mustofa, Imam; Madyawati, Sri Pantja; Rimayanti, Rimayanti; Safitri, Erma; Hermadi, Herry Agoes
Jurnal Medik Veteriner Vol. 5 No. 2 (2022): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol5.iss2.2022.207-212

Abstract

This study was conducted to know the influence of grass to concentrate ratio to milk urea nitrogen (MUN) level, progesterone level and pregnancy status in Friesian Holstein. A total of 18 dairy cows were randomly selected from the population. The cows were grouped based on feed and MUN and pregnancy. Milk sample collection was carried out once on the seventh day after estrus (D7) and tested with the Barthelot method for measuring urea nitrogen levels. Blood samples were taken three times, on the day of estrus (D0), seven days after estrus (D7), and twenty-two days after estrus (D22), and were tested using Immuno-Sorbent Assay (ELISA). Pregnancy examination was carried out at D90 after artificial insemination through rectal palpation. Based on the analysis, grass to concentrate ratio, progesterone level and MUN level showed no significant difference (p>0.05) but it showed significantly different on milk yield (p<0,05). Based on pregnancy status, progesterone level at D22 showed significantly different whereas at D0 and D7 showed insignificantly different. MUN level and grass to concentrate level also showed significantly different. It can be concluded that the ratio of grass and concentrate did not increase MUN level, progesterone level and pregnancy rate.
Protective Effect of Ethanol Extract of Kesum Leaves (Polygonum minus) on the Number of Leydig Cells in Mice Exposed to Cadmium Chloride Paramita, Chici Ayu; Plumeriastuti, Hani; Madyawati, Sri Pantja; Arimbi, Arimbi; Mulyati, Sri; Kurnijasanti, Rochmah
Jurnal Medik Veteriner Vol. 6 No. 1 (2023): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss1.2023.43-50

Abstract

This study aimed to determine the protective effect of ethanol extract of kesum leaves (Polygonum minus) on the number of Leydig cells in mice exposed to cadmium chloride (CdCl2). A total of 20 male mice were divided into five groups, each group consisting of four replications and then given different treatments orally for 21 days. The treatment groups included (K-) 0,5% CMC Na suspension + distilled water, (K+) 0,5% CMC Na suspension + 12 mg/kgBW/day cadmium chloride, (P1, P2, and P3) ethanol extract of kesum leaves, respectively. -respectively 200, 400, and 800 mg/kgBW/day + 12 mg/kgBW/day cadmium chloride. Observations were made by counting the number of Leydig cells. Data were analyzed by ANOVA and Duncan's test. The results showed significant differences (p<0,05) between groups P1, P2, P3 and K+. On the other hand, the P2 group showed similar results to K-. In conclusion, the ethanol extract of kesum leaves can protect and maintain the number of Leydig cells in the testes of mice from exposure to cadmium chloride, and the optimal dose was 400 mg/kgBW/day.
PEMBERIAN EKSTRAK MENIRAN (Phyllanthus niruri Linn) TERHADAP GAMBARAN HISTOPATOLOGI INFUNDIBULUM AYAM PETELUR YANG DIINFEKSI Escherichia coli Irawan, Adinda Riezki; Sabdoningrum, Emy Koestanti; Hidanah, Sri; Chusniati, Sri; Madyawati, Sri Pantja; Tehupuring, Benjamin Christoffel
Journal of Basic Medical Veterinary Vol. 8 No. 1 (2019): Journal of Basic Medical Veterinary, June 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i1.19992

Abstract

The purpose of this research aimed to prove the effect of meniran extract (Phyllanthus niruri Linn) on layer hen infected with Escherichia coli to reduce inflammation and congestion on infundibulum histopathological view.  This research used 20 samples of layer hen. The treatment in this research was P0- (not infected with Esherichia coli and did not treated with meniran(Phyllanthus niruri Linn)), P0+ (infected with Escherichia coli and did not treated with meniran(Phyllanthus niruri Linn)), P1 (infected with Escherichia coli and treated with 10% meniran extract (Phyllanthus niruri Linn)), P2 (infected with Escherichia coli and treated with 20% meniran extract (Phyllanthus niruri Linn)), P3 (infected with Escherichia coli and treated with 30% meniran extract (Phyllanthus niruri Linn)). The data of this study analyzed by Kruskal-wallis and followed with Mann-withney test. The result of this research can be concluded that 10% concentration of meniran extract (Phyllanthus niruri Linn) was effective to eliminate Escherichia coli, 20% concentration of meniran extract (Phyllanthus niruri Linn) able to promote antibacterial activity, and 30% concentration of meniran extract shown antibacterial rate on high potential so it can be used to reduce inflammation and congestion as the result of Escherichia coli infection.
Co-Authors Aftabuddin Rz, Muhammad Agung Budianto Achmad, Agung Budianto Agustono, Bodhi Ali Saifudin Arimbi Aryaloka, Suhita Benjamin Christoffel Tehupuring, Benjamin Christoffel Budi Utomo Chrisnahutama, Adrian Chusniati, Sri Dikman, Dicky Mohammad Erma Safitri Fajarwati, Rini Gandul Atik Yuliani Hani Plumeriastuti Hardaningtyas, Enggar Hariyono Hariyono Heni Puspitasari Herry Agoes Hermadi Hidanah, Sri IMAM MUSTOFA Irawan, Adinda Riezki Iskandari, Novia Nur Ismudiono Ismudiono, Ismudiono Kadek Rachmawati Kurniawan, Muhammad ‘Ahdi Kusnoto Lailiyah, Fahlevi Leo Yosdimyati Romli Luqman, Epy Muhammad Masyitoh, Herlina Mirni Lamid Mufasirin Ni Made Sukartini Nidom, Chairul Anwar Paramita, Chici Ayu Pradana, Giffari Danindra Praja, Ratih Novita Prasetyo, Ivo Febrina Prastiya, Ragil Angga Pudji Srianto Purwono, Rudi Renaldi, Nogi Rimayanti Rimayanti Rochmah Kurnijasanti Sabdoningrum, Emy Koestanti Saputro, Amung Logam Sarmanu, Sarmanu Seagames Waluyo, Seagames Shifa Fauziyah Sri Mulyati Sukmanadi, Mohammad Sunarso, Agus Supriyadi Supriyadi Tan, Chin Xuan TARZAN PURNOMO Teguh Hari Sucipto, Teguh Hari Tita Damayanti Lestari Tri Suwanti, Lucia Tri Wahyu Suprayogi Trilas Sardjito Wibawati, Prima Ayu Widiyatno, Thomas Valentinus Widjiati Widjiati, Widjiati Widya Paramita Lokapirnasari Yeni Dhamayanti