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Probiotic Potential of Lactic Acid Bacteria on Feed Efficiency, Weight and Carcass Percentage in Ducks Chandra, Evania Haris; Lokapirnasari, Widya Paramita; Hidanah, Sri; Al-Arif, Muhammad Anam; Yuniarti, Wiwik Misaco; Luqman, Epy Muhammad
Jurnal Medik Veteriner Vol. 5 No. 1 (2022): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol5.iss1.2022.69-73

Abstract

The aim of the study was to determine the effect of the use of probiotic lactic acid bacteria (Lactobacillus acidophilus, Lactobacillus casei, Lactococcus lactis, and Bifidobacterium sp) on feed efficiency, carcass weight and carcass percentage in broiler ducks. Probiotics lactic acid bacteria produce bacteriocins and organic acids that inhibit the growth of pathogenic microbes in the digestive tract of poultry, so that the intestinal villi of broiler ducks are healthy. Besides that, probiotics are able to reduce cholesterol levels in meat when consumed by humans and can improve health. This study used 48 peking ducks. The probiotics of Lactic Acid Bacteria used were a concentration of 1.2 x 108 CFU/ml. In this study there were four different treatments, namely P0 (without probiotics), P1 (given 1 ml probiotics/liter drinking water), P2 (given 2 ml probiotics/liter drinking water) and P3 (given 3 ml probiotics/liter of drinking water). The results of the feed efficiency study showed that there was a significant difference (p<0.05) in the P0, P1, P2 and P3 treatments. The highest feed efficiency was at P3 (33.81%) while the lowest was P0 (30.84%). The results of the study on carcass weight indicated that the probiotics tended to be higher than those who were not given probiotics but statistically not significantly different (p>0.05). The highest carcass weight was P3 (1749.92 g) and the lowest was P0 (1709.25 g). The results of the study on the percentage of carcass weight showed a significant difference (p<0.05) in both P0, P1, P2 and P3 treatments. The highest percentage of carcass weight was at P3 (64.60%) and the lowest was P0 (60.96%). It can be concluded that the administration of probiotics lactic acid bacteria (Lactobacillus acidophilus, Lactobacillus casei, Lactococcus lactis, and Bifidobacterium sp) at a dose of 1 ml/liter of drinking water, 2 ml/liter of drinking water and 3 ml/liter of drinking water can affect feed efficiency and the percentage of duck carcasses. but has no effect on the carcass weight of broiler ducks.
Efficacy of Lactic Acid Bacteria Probiotics on Feed Efficiency and Carcass Weight in Kampung Unggul Balitbangtan (KUB) Chicken Berlina, Cyrcilia Relita; Hidanah, Sri; Al Arif, Muhammad Anam; Luqman, Epy Muhammad; Yuniarti, Wiwik Misaco; Mufasirin, Mufasirin
Jurnal Medik Veteriner Vol. 6 No. 3 (2023): Special Issue: International Conference on Veterinary Medicine and Health Scien
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss3.2023.116-121

Abstract

This study aimed to determine the effect of lactic acid bacteria probiotics on the performance of Kampung Unggul Balitbangtan (KUB) chicken. A total of 48 KUB chickens aged 2 weeks were randomized into 4 treatments and each treatment consisted of 12 chickens. This study was conducted for 8 weeks. This study used 4 different treatments including (P0) not using probiotics, (P1) using probiotics of 1 mL/L drinking water, (P2) using probiotics of 2 mL/L drinking water, and (P3) using probiotics of 3 mL/L drinking water. The data were analyzed using ANOVA continued with Duncan's Multiple distance test. As a result, reported that the data had a significant difference in the increase in feed efficiency and carcass weight. In conclusion, the administration of probiotics of 3 mL/L of drinking water can increase feed efficiency and carcass weight in KUB chickens.
EFEK PEMBERIAN EKSTRAK RUMPUT KEBAR (Biophytum petersianum Klotzsch) TERHADAP JUMLAH SEL LEYDIG MENCIT (Mus musculus) JANTAN YANG DIPAPAR 2,3,7,8-TETRACHLORODIBENZO-P-DIOXIN Nursadida, Milla; Plumeriastuti, Hani; Dhamayanti, Yeni; Widjiati, Widjiati; Luqman, Epy Muhammad; Arimbi, Arimbi
Journal of Basic Medical Veterinary Vol. 8 No. 1 (2019): Journal of Basic Medical Veterinary, June 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i1.19998

Abstract

This study was aim to examine the effect of kebar grass extract (Biophytum petersianum Klotzsch) to against number leydig cells of mice (Mus musculus) by exposed 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD). Twenty five male mice (Mus musculus) 4 months with avarage body weight 20 g were used. These animals were divided into five groups (K(-), K(+), P1, P2 and P3). K(-) was treated with placebo, K(+) was treated by exposed TCCD single dose injection intaperitoneal 7µg/KgBw, P1 was treated by exposed TCCD single dose injection intaperitoneal 7µg/KgBw and kebar grass extract 0,045 mg/g Bw/day P2 was treated by exposed TCCD single dose injection intaperitoneal 7µg/KgBw and kebar grass extract 0,080 mg/g Bw/day, P3 was treated by exposed TCCD single dose injection intaperitoneal 7µg/KgBw and kebar grass extract 1,350 mg/g Bw/day. This research has been conducted for 53 days. The data were compared using ANOVA and Duncan test by SPSS 22.4 for windows. The result showed that Kebar Grass Extract in all of groups can prevent the damage of leydig cells in testis that exposed by TCCD significantly (p<0,05) and kebar grass extract 0,135 mg/kgBw/day can increase amount of leydig cells maximaly.
PENGARUH PEMBERIAN EKSTRAK KULIT PISANG KEPOK (Musa acuminata) TERHADAP GAMBARAN HISTOPATOLOGI BRONKUS DAN VENA PULMONALIS MENCIT (Mus musculus) JANTAN YANG DIPAPAR ASAP ROKOK Jeanita, Winny; Meles, Dewa Ketut; WIdjiati, Widjiati; Hamid, Iwan Sahrial; Luqman, Epy Muhammad; Arimbi, Arimbi
Journal of Basic Medical Veterinary Vol. 9 No. 1 (2020): Journal of Basic Medical Veterinary, June 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v9i1.21089

Abstract

This research aimed to attest the effect of Kepok banana (Musa acuminata) peel extract on the histopathology of bronchus and pulmonary veins in male mice (Mus musculus) exposed by cigarette smoke. The 24 experimental male mice were divided into six groups with each treatment having four replicate. All the groups, except K- as a control group, was treated with cigarette smoke for 14 days. After that, K- and K+ was treated with CMC-Na 1%, KO was treated with ascorbic acid 13 mg/kgBW, P1 was treated with Kepok banana peel extract 14 mg/kgBW, P2 was treated with Kepok banana peel extract 28 mg/kgBW, and P3 was treated with Kepok banana peel extract 56 mg/kgBW for 14 days. The data of the thickness of bronchial epithelium and the diameter of pulmonary veins were analyzed using ANOVA (Analysis of Varience) with significance p<0,05 and continued with Post- hoc Tukey. The result of the thickness of bronchial epithelium showed that K+ had the highest thickening (431,83 + 9,71 µm) and significant differences (P<0,05) with all the groups. The result of P3 showed the lowest thickening of bronchial epithelium (170,84 + 2,70 µm). The result of pulmonary veins diameter showed that K+ had the narrowest diameter (525,64 + 16,61 µm) and significant differences (p<0,05) with all the groups. The result of P3 showed the lowest diameter narrowing of pulmonary veins (718,10 + 9,64 µm). The conclussion of this research are Kepok banana peel extract able to decreased the thickness of bronchial epithelium and the narrowing of pulmonary veins diameter in male mice with effective dose 28 mg/ kgBW.
PENGARUH PEMAPARAN KARBOFURAN PADA INDUK MENCIT (Mus musculus) TERHADAP GAMBARAN HISTOPATOLOGI GINJAL ANAK MENCIT MASA LAKTASI Purnomo, Edi; Luqman, Epy Muhammad; Ratnani, Hermin; Plumeriastuti, Hani; Mafruchati, Maslichah; Dhamayanti, Yeni
Journal of Basic Medical Veterinary Vol. 9 No. 1 (2020): Journal of Basic Medical Veterinary, June 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v9i1.21095

Abstract

Carbofuran is a broad spectrum carbamat insecticide which is used in various farm practices. An exposure of carbofuran has been reported able to induce Reactive Oxygen Species (ROS) or free radical in kidney. Free radical caused cells damage. The present study was designed to investigate the effect of carbofuran in kidneys histopathologic of mice pup whose dam were exposed by carbofuran during the lactation period. High levels of ROS can bind to various biomolecules such as proteins, lipids and nucleic acids so that carbofuran has a negative effect on tissue functions and structures. Free radical caused changes in the pathological conditions of the tissue including the kidneys. The dams were devided into four groups of five animals each. P0 animal group was received aquadest 0,1 ml per body weight (BW), P1 animal group was treated carbofuran at the dose of 1/16 LD50 (0,3125 mg per BW), P2 animal group was treated carbofuran at the dose of 1/8 LD50 (0, 625 mg per BW), and P3 animal group was treated carbofuran at the dose of 1/4 LD50 (1,25 mg per BW). The carbofuran was exposed via oral postnatal day 1st until 9th postnatal day after delivery. At postnatal day 10th, offspring were sacrified. The kidney of mice pup was collected and processed for microscopic examination with hematoxilin-eosin staining. Microscopic observations on the histology of the mice pup kidney showed an increase in the degree of kidney damage as an increase in the dose of carbofuran given to the dams. The results showed tubular changes in the form of tubular degeneration, inflammatory cell infiltration and tubular necrosis in the kidney of mice pups.
Pemberdayaan Kelompok Tani Melalui Pemanfaatan Ampas Tahu Sebagai Bahan Dasar Pakan Kelinci Di Desa Wonosalam Kab Jombang Wijoyo, Suparto; Luqman, Epy Muhammad; Aisyah R.r, Herini Siti; Zariyah, Aminatus; Rianda, Juan Safero; Malae, Rafyan
Semeru: Jurnal Pengabdian Kepada Masyarakat Volume 1 Nomor 2 Tahun 2024
Publisher : LPPM Universitas Bhayangkara Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55499/semeru.v1i02.1166

Abstract

Kelompok sasaran dalam program ini adalah kelompok buruh tani yang secara sambilan memelihara kelinci dengan pakan dari rumput sekitar kebun majikannya, Kelompok mitra mengalami hambatan dalam penyediaan pakan kelinci ketika musim kemarau karena rumput mulai lanka sehingga kelinci dijual dengan harga murah. Ampas tahu sebagai bahan dasar pakan kelinci diharapkan dapat diolah menjadi pakan kelinci sehingga budidaya kelinci dapat dikelola secara intensif. Tujuan dari program ini adalah memberdayakan kelompok tani untuk dapat budidaya kelinci secara intensif dapat menjadi usaha yang menjanjikan untuk dapat memenhi kebuhan hidupnya Metode untuk menyelesaiakan masalah untuk mencapai tujuan dan memperoleh target program ini maka digunakan metode pelatihan dan praktek serta pendampingan.
Detection of Anti-Müllerian Hormone (AMH) mRNA in Serum and Ovarian Tissue of Local Indonesian Cattle Using EvaGreen-based RT–qPCR Dikman, Dicky Mohammad; Puspitasari, Heni; Waluyo, Seagames; Kurniawan, Muhammad ‘Ahdi; Supriyadi, Supriyadi; Srianto, Pudji; Luqman, Epy Muhammad; Suprayogi, Tri Wahyu; Madyawati, Sri Pantja; Safitri, Erma; Lestari, Tita Damayanti
Jurnal Medik Veteriner Vol. 8 No. 2 (2025): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss2.2025.382-397

Abstract

Anti-Müllerian hormone (AMH) has been identified as a potential biomarker for assessing ovarian reserve and reproductive capacity in cattle, with high heritability and relatively stable expression. However, studies detecting AMH mRNA in bovine serum remain scarce due to the matrix’s inherently low RNA yield, susceptibility to degradation, and potential presence of PCR inhibitors. This study provides an exploratory validation of AMH mRNA detection in serum — a challenging matrix compared with ovarian tissue — using EvaGreen-based reverse transcriptase–quantitative PCR (RT–qPCR). Specific primers for the AMH and β-actin genes were designed in silico and validated through melting curve analysis and linearity testing. The results showed high amplification efficiency (AMH: 100.2%, R² = 0.994; β-actin: 109.1%, R² = 0.996), with specific amplification of both targets. AMH detection in serum samples was successful in some samples, while the β-actin gene was consistently amplified as a reference gene. Despite the low RNA quality from serum and the presence of organic contaminants, the method demonstrated its feasibility for detecting AMH transcripts in a minimally invasive manner. Physiologically, AMH levels positively correlate with antral follicle count, superovulation success, and embryo quality, and are sensitive to heat stress and other environmental factors. These findings provide a foundational basis for developing molecular diagnostic approaches based on AMH gene expression in cattle reproductive management programs and support the future development of efficient, accurate, and context-specific biomolecular-assisted selection technologies for tropical livestock systems.
Pengaruh injeksi nikotin terhadap motilitas, viabilitas, dan integritas membran spermatozoa mencit (Mus Musculus) Pramudita, Kautsar Pandu; Lokapirnasari, Widya Paramita; Susilowati, Suheni; Ratnani, Hermin; Safitri, Erma; Luqman, Epy Muhammad
Ovozoa: Journal of Animal Reproduction Vol. 9 No. 3 (2020): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v9i3.2020.77-81

Abstract

The process of spermatogenesis is sensitive to nicotine, a main component of cigarette smoke. This study aimed to observe the effect of nicotine injection on motility, viability, and membrane integrity of the spermatozoa of mice (Mus musculus). The mice were 10 weeks old, weighing about 20 grams, and in healthy and fertile conditions. Mice were adapted for 1 week by being given feed in the form of pellets and drinking water every day. Twenty of the mice were divided into four groups. Group K as a control was injected with 0.9% NaCl at 2.5 mg/Kg bw. Group P1 was injected with nicotine at 2.5 mg/Kg bw. P2 was injected with nicotine at 5 mg/Kg bw. Group P3 was injected with nicotine at 10 mg/Kg bw. The injection was carried out subcutaneously as much as 0.5 ml once a day for 35 days. Data analysis used the Analysis of Variances (ANOVA), followed by Duncan's Multiple Range Test. The results showed that the viability, motility, and membrane integrity of the spermatozoa decreased significantly (p <0.05) according to the increase in the dose of nicotine injected. It could be concluded that subcutaneous nicotine injection linearly decreased the motility, viability, and membrane integrity of the spermatozoa of mice.
Erythrocyte, Hemoglobin, and Hematocrit Profiles in Indonesian Simental and Limousin in Highlands and Lowlands in Banyuwangi Trisnawati, Sharfina Habibah; Safitri, Erma; Agustono, Bodhi; Luqman, Epy Muhammad; Saputro, Amung Logam; Prastiya, Ragil Angga
Journal of Basic Medical Veterinary Vol. 14 No. 2 (2025): Journal of Basic Medical Veterinary, December 2025
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v14i2.78209

Abstract

This study aimed to evaluate the differences in blood profile of erythrocytes, hemoglobin, and PCV in Indonesian simental and limousin beef cattle in highlands and lowlands. Banyuwangi is one of the beef cattle contributors in East Java with its highland and lowland topography. This research was conducted from July to August 2023. The research was conducted in 3 locations namely highlands (Kalibaru), lowlands (Rogojampi) and sample examination in Airlangga University Banyuwangi. Animals used in this study were Indonesian simental and limousin beef cattle with 1-2 years of age as many as 15 heads in the highlands and 15 heads in the lowlands. The research method used was purposive sampling. Blood samples used in this study used Indonesian simental and limousin beef cattle blood taken using venoject and inserted in EDTA tubes and examined using a Hematology Analyzer. The variables observed in this study were the number of erythrocytes, hemoglobin, and PCV. The data obtained were processed with Independent T-test data analysis. The results showed no significant difference in erythrocytes and PCV, but there was a significant difference in hemoglobin levels. The conclusion of this study is that Indonesian simental and limousin beef cattle in the lowlands experience mild anemia.
ANALYSIS OF ANTIBIOTIC COST IN BROILER MANAGEMENT PRACTICE IN SUBANG REGENCY, WEST JAVA, INDONESIA Sugiyono; Sabdoningrum, Emy Koestanti; Hidanah, Sri; Lokapirnasari, Widya Paramita; Luqman, Epy Muhammad; Hestianah, Eka Pramyrtha
Jurnal Biosains Pascasarjana Vol. 27 No. 2 (2025): JURNAL BIOSAINS PASCASARJANA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbp.v27i2.2025.144-149

Abstract

Broiler farms are generally susceptible to disease attacks caused by viruses, bacteria, parasites, fungi, the environment, and deficiencies in nutritional elements. The use of antibiotics in the livestock industry generally aims to treat livestock so as to reduce the risk of death and restore livestock to a healthy condition. There are three patterns for rearing broiler chickens in Indonesia, especially in West Java, namely modern rearing using a closed house system, semi closed house and conventional rearing using open house system. This study aims to prove the analysis of the cost of antibiotics and their market potential needs to be known so that rational and balanced use of antibiotics can be illustrated with the benefits to the results of raising broiler livestock. The design used in this research was a purposive sampling. The variables in this research are the closed house, semi closed house and open house broiler farming systems. The independent variables in this research are the cost of antibiotics each bird per period. Descriptive analysis is used to obtain stewardship antibiotics based on the existing medication program. The antibiotic cost each bird per period from closed house amount is Rp. 70,49 + 38,65, semi closed house is Rp. 100,20 + 42,28, and open house is Rp. 130,32 + 64,68. The means of antibiotic cost each birds per period amount is Rp. 100,34 + 54,17 use a factor for forecasting market potency of antibiotic. The market potency per year in Subang regency amount Rp. 5.799.005.710 + 3.130.677.091, West Java Province amount Rp. 74.165.707.957 + 40.039.429.938, Indonesia amount Rp. 312.461.530.889 + 168.686.875.905. Apart from that, antibiotic stewardship on a broiler farm scale is also needed to get an idea regarding the appropriate use of antibiotics in broiler farms, especially in the Subang district area of West Java Province.