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Diversity of Fusarium Endophytes Isolated from Wild Bananas in Pandenglang, Indonesia Nani Maryani; Siti Yulianti; Indriati Ramadhani; Rida Oktorida Khastini; Suroso Mukti Leksono
Journal of Tropical Biodiversity and Biotechnology Vol 8, No 1 (2023): April
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.76897

A group of Fusarium spp., in the Fusarium oxysporum species complex is known as pathogens on bananas, i.e., Fusarium wilt or Panama Disease. However, many Fusarium spp. are also known to be endophytes inside healthy banana plants and have been less explored and investigated. Fusarium endophytes have been demonstrated to be effective against the Fusarium pathogen that causes wilting in some crops such as tomatoes and watermelon. Thus, we explored endophytes Fusarium from local bananas in Pandenglang Banten for further use as biocontrol of Fusarium wilt. Four wild banana accessions were identified, from which 9 Fusarium isolates recovered from its pseudostems asymptomatic plants. All isolates were characterized based on their morphological characters and sequence of the Internal Transcribed Spacer (ITS) gene. These isolates belong to four complexes of Fusarium i.e. Fusarium equiseti species complex, Fusarium oxysporum species complex, Fusarium sambucinum species complex, and Fusarium solani species complex (currently described as Neocosmospora). Further study on molecular characterization of these isolates using specific genes and their potential antagonists of pathogens still needs to be discovered for other use as a biocontrol against Fusarium wilt.

Bananas and Their Wild Relatives in Pandeglang, Indonesia Susilawati Dewi; Fajarudin Ahmad; Rida Oktorida Khastini; Suroso Mukti Leksono; Nani Maryani
HAYATI Journal of Biosciences Vol. 30 No. 6 (2023): November 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.6.1071-1091

Pandeglang is one of the banana producer regencies of Banten, Indonesia, that produce 924 tons per year. However, information regarding the diversity of banana germplasm in this region is unavailable. This study explored districts Cadasari, Banjar, Jiput, Labuan, and Menes. Morphological characters were noted and used to identify the cultivars, subspecies, or varieties. In total, 22 accessions were collected from 22 collection sites. Based on morphological characters, we described 14 banana accessions of 10 cultivars, three accessions of wild Musa acuminata and one of Musa balbisiana. Considering its genome composition, five genome types we described among these banana cultivars, i.e., AA (divided into AA and AA wild), BB, AAA, AAB, and ABB. In this study, we did a principal component analysis and saw that AA wild and AAB were clearly separated, but the other genome types were clustered partially or included in one cluster. Our study expands the information on banana diversity in Indonesia. Further study on the potential of this banana germplasm to be used as a genetic resource to find resistance to banana diseases is needed.

Keanekaragaman Fungi Entomopatogen Lokal Asal Cagar Alam Gunung Tukung Gede Rida Khastini; Nani Maryani; Iing Dwi Lestari; Ika Rifqiawati; Nada Ummatul Millah
BIOEDUSCIENCE Vol 5 No 2 (2021): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/j.bes/526004

Background: Entomopathogenic fungi are one of the potential biodiversity assets to be used as biological control agents. However, information about the diversity of entomopathogenic fungi, especially in the Gunung Tukung Gede (GTG) nature reserve is very limited. The purpose of this study was to provide information on the biodiversity of entomopathogenic fungi in the GTG nature reserve. Methods: The entomopathogenic fungi were explored from 3 different stations: primary forest, secondary forest and disturbed forest. Entomopathogenic fungi were isolated identified both macroscopically and microscopically. Results: A total of 15 specimens of entomopathogenic fungi consisting of 5 families and 8 types of fungi successfully isolate. The entomopathogen fungi were Basidiobolus haptosporus, Beauveria bassiana., Metarhizium aniesophalie, Paecilomyces sp., Aschersonia sp., Aspergillus sp. 1, Aspergillus sp.2, and Septobasidium sp. These fungi infect insect hosts from the orders Lepidoptera, Hemiptera and Homoptera. The index of the diversity of entomopathogenic fungi at 3 consecutive stations is 1.5495; 1.3322; and 0.6365 (medium category). Conclusions: The GTG Nature Reserve has a unique diversity of entomopathogenic fungi. Therefore, further research is needed in order to determine the diversity and potential utilization of the existing entomopathogenic fungi.

Keanekaragaman Fungi Entomopatogen Lokal Asal Cagar Alam Gunung Tukung Gede Rida Khastini; Nani Maryani; Iing Dwi Lestari; Ika Rifqiawati; Nada Ummatul Millah
BIOEDUSCIENCE Vol 5 No 2 (2021): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/j.bes/526004

Background: Entomopathogenic fungi are one of the potential biodiversity assets to be used as biological control agents. However, information about the diversity of entomopathogenic fungi, especially in the Gunung Tukung Gede (GTG) nature reserve is very limited. The purpose of this study was to provide information on the biodiversity of entomopathogenic fungi in the GTG nature reserve. Methods: The entomopathogenic fungi were explored from 3 different stations: primary forest, secondary forest and disturbed forest. Entomopathogenic fungi were isolated identified both macroscopically and microscopically. Results: A total of 15 specimens of entomopathogenic fungi consisting of 5 families and 8 types of fungi successfully isolate. The entomopathogen fungi were Basidiobolus haptosporus, Beauveria bassiana., Metarhizium aniesophalie, Paecilomyces sp., Aschersonia sp., Aspergillus sp. 1, Aspergillus sp.2, and Septobasidium sp. These fungi infect insect hosts from the orders Lepidoptera, Hemiptera and Homoptera. The index of the diversity of entomopathogenic fungi at 3 consecutive stations is 1.5495; 1.3322; and 0.6365 (medium category). Conclusions: The GTG Nature Reserve has a unique diversity of entomopathogenic fungi. Therefore, further research is needed in order to determine the diversity and potential utilization of the existing entomopathogenic fungi.

Enhancing Student’s Communication Skills in Writing Biology Practical Reports Using Flashcards Khairunnissa, Afifah Zulfa; Fitriana, Desi Eka Nur; Rifqiawati, Ika; Maryani, Nani
Biodidaktika : Jurnal Biologi dan Pembelajarannya Vol 19, No 2 (2024)
Publisher : Universitas Sultan Ageng Tirtayasa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30870/biodidaktika.v19i2.24455

This study aimed to determine students' written communication skills in writing practicum reports using flashcards. The research was conducted on grade 11th high school students with a simple random sampling technique. The research method used was an experimental method with Posttest-Only Control Group Design. In the experimental group, students were instructed to use flashcards to write their practicum reports, while the control group used conventional methods, writing on paper as usual. Students were then asked about their experience with a questionnaire. Students' reports on flashcards were scored and analyzed. The results showed that using flashcards significantly improved students’ written communication skills in writing practicum reports. This improvement was reflected in comparing post-test results between the experimental and control groups. In addition, students also responded positively to flashcards to improve their practicum report writing skills. The findings of this study contribute to the practical and theoretical understanding of effective teaching methods for writing, especially in the context of practicum reports. This study gives an insight into students' communication skills in writing that can be improved by applying innovative learning strategies.

KOMUNITAS FUNGI RIZOSFER PISANG LOKAL DI PANDEGLANG Siti Nursaffanah Hasna; Nani Maryani; Rida Oktorida Khastini; Iing Dwi Lestari; Yeyen Maryani; Dwi Ratnasari
Jurnal Agrotek Tropika Vol 12, No 3 (2024): JURNAL AGROTEK TROPIKA VOL 12, AGUSTUS 2024
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jat.v12i3.8405

Tanaman sehat umumnya kaya akan komunitas fungi rizosfer. Beberapa penelitian melaporkan tingginya diversitas fungi pada bagian rizosfer tanaman hortikultura mampu menekan penyakit sehingga tanaman lebih tahan. Penyakit layu fusarium atau Panama Disease merupakan penyakit utama pada budidaya pisang. Pisang yang tahan pada tanah terkontaminasi umumnya kaya akan mikroba rizosfer. Penelitian ini bertujuan membandingkan komunitas fungi rizosfer pada tanaman yang terserang Fusarium dengan tanaman sehat pada pisang-pisang lokal di Kabupaten Pandeglang, Banten. Sampel diambil di enam kecamatan yaitu Cadasari, Banjar, Menes, Pandeglang, Saketi, dan Cisata. Sampel yang diambil berasal dari rizosfer pisang yang bergejala layu fusarium (symptomatic plants) dan yang lebih tahan, tidak menunjukkan gejala (asymptomatic plants). Identifikasi tanaman yang sakit menggunakan deteksi cepat dengan PCR. Isolasi fungi dilakukan dengan metode pengenceran berseri pada medium agar dekrosa kentang. Isolat fungi yang telah dimurnikan dan dibuat biakan kultur spora tunggal kemudian diidentifikasi secara morfologi menggunakan mikroskop dengan perbesaraan 1000x. Total 63 isolat fungi berhasil diisolasi dan diidentifikasi, terdiri dari tujuh genus: Aspergillus, Curvularia, Fusarium, Gongronella, Mucor, Penicillium, dan Purpureocillium. Komposisi fungi dari kedua jenis rizosfer tidak jauh berbeda, namun keragaman species fungi pada tanaman pisang tanpa gejala lebih tinggi dibanding pisang yang terserang fusarium. Aspergillus dan Fusarium adalah fungi yang paling banyak ditemukan. Penelitian ini memberikan informasi komposisi dan keragaman species fungi pada rizosfer pisang lokal yang terserang maupun yang lebih tahan penyakit layu fusarium. Studi molekuler yang dapat menghitung kelimpahan species dan dinamikanya selama proses terjadinya infeksi diperakaran akan membantu manajemen penyakit layu fusarium untuk menciptakan ekosistem tanah yang dapat menekan penyakit.

Optimalisasi Pemanfaatan Media Pembelajaran Berbasis TIK Melalui Program Kampus Mengajar bagi Sekolah Terpencil di Kabupaten Lebak Rida Oktorida Khastini; Nani Maryani; Dinar Sugiana; Akhmad Baihaqi
Jurnal Pengabdian UNDIKMA Vol. 3 No. 3 (2022): November
Publisher : LPPM Universitas Pendidikan Mandalika (UNDIKMA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/jpu.v3i3.6004

This community service activity aims to optimize the use of information and communication technology (ICT)-based learning media through campus teaching programs to improve the quality of education in remote areas. The method of implementing this service is with training which includes the stages of preparation, implementation, and evaluation of results through filling out questionnaires by teachers and students. This service partner is SMAN 1 Malingping as one of the remote school areas in Lebak district. The results of this dedication show that partners, namely teachers and students, feel the positive impact of using ICT application-based learning media in the classroom. Teachers' skills in implementing ICT-based learning media and students' motivation to learn increases. In addition, teachers have succeeded in overcoming various challenges in the learning process by optimizing the use of ICT-based learning media.

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