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All Journal Jurnal Farmasi Udayana Media Farmasi Jurnal Mandala Pengabdian Masyarakat Journal of Experimental and Clinical Pharmacy (JECP) Jurnal Pengabdian Kefarmasian Student Scientific Journal Media Farmasi
Pakadang, Sesilia Rante
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Humanities Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary

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Journal : Media Farmasi

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Aktivitas Vermisida Ekstrak Daun Miana Terhadap Ascaris Lumbricoides dengan Metode Real Time Pakadang, Sesilia Rante; Ratnah, St.; Salasa, Alfrida Monica; Karim, Djuniasti
Media Farmasi Vol 19 No 2 (2023): Media Farmasi Edisi Oktober 2023
Publisher : Jurusan Farmasi Poltekkes kemenkes Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32382/mf.v19i2.168

Abstract

Soiled Transmitted Helminth (STH) is an infectious disease caused by earthworms. The type of worm identified as the cause of STH is dominated by Ascaris lumbricoides and 3 other types. Many studies have been carried out to monitor the results of worm death. This study aimed to determine the profile of vermicides including onset of action, duration, and effectiveness of miana leaf extract compared to the drug pyrantel pamoat. The research method is to gradually start preparing miana leaf extract using a maceration process with continuous stirring, preparing the extract, and treating worms. The worms are placed in a sterile cup, then the test material is added and observed every hour. Worm death profiles such as paralysis, death, and life were observed for each worm subject. The results showed the death profile of worms after administration of miana leaf extract concentrations of 10, 20, and 40 mg/ml and pyrantel pamoat 2.5 mg/ml gave onset of action data for 4, 3, 2, and 1 hour respectively. The working duration of the test material is 5, 5, 3, and 1 hour respectively.  The effective dose is generated by a concentration of 40 mg/ml which can kill worms starting 2 hours after administration of the test material, with a duration of 1 hour  Soiled Transmitted Helminth (STH) adalah salah satu penyakit infeksi akibat cacing tanah. Jenis cacing yang teridentifikasi sebagai penyebab STH didominasi oleh Ascaris lumbricoides dan 3 jenis lainnya, penelitian telah banyak dilakukan dengan memantau hasil akhir kematian cacing. Tujuan penelitian ini mengetahui profil vermisida meliputi onset of action, durasi dan efektivitas ekstrak daun miana yang dibandingkan dengan obat pyrantel pamoat. Metode penelitian secara bertahap mulai penyiapan ekstrak daun miana dengan proses maserasi dengan pengadukan kontinu, penyiapan ekstrak dan perlakuan terhadap cacing. Cacing dimasukkan dalam cawan steril, kemudian ditambahkan bahan uji dan diamati setiap jam. Profil kematian cacing seperti paralisis, mati dan hidup diamati setiap subyek cacing. Hasil penelitian menunjukkan profil kematian cacing setelah pemberian ekstrak daun miana konsentrasi 10, 20 dan 40 mg/ml dan pyrantel pamoat 2,5 mg/ml memberikan data onset of action berturut 4, 3, 2 dan 1 jam. Durasi kerja bahan uji berturut 5, 5, 3 dan 1 jam. Dosis efektif dihasilkan oleh konsentrasi 40 mg/ml yang dapat mematikan cacing mulai 2 jam setelah pemberian bahan uji, durasi 1 jam.
Isolasi, Identifikasi, Dan Aktivitas Antibakteri Fungi Endofit Daun Pegagan (Centella asiatica L.) Terhadap Staphylococcus epidermidis Dan Propionibacterium acnes: Isolation, Identification and Antibacterial Activity Of Fungi Endophyte Of Pegagan Leaves (Centella asiatica L.) against Staphylococcus epidermidis and Propionibacterium acnes Pakadang, Sesilia Rante; Ayuasrini, Ayuasrini; Ratnah, St.; Salasa, Alfrida Monica
Media Farmasi Vol 21 No 1 (2025): Media Farmasi Edisi April 2025
Publisher : Jurusan Farmasi Poltekkes kemenkes Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32382/mf.v20i2.600

Abstract

Pegagan (Centella asiatica L.) is widely known as a traditional medicine with various properties, including as an antibacterial. This study aims to isolate endophytic fungi from Pegagan leaves that have the potential to produce antibacterial secondary metabolites against Staphylococcus epidermidis and Propionibacterium acnes. Isolation was carried out by leaf sterilization and inoculation on SDA media, followed by fermentation and extraction using ethyl acetate. Antibacterial activity tests were carried out using the agar diffusion method. Five isolates were obtained: PTH (Cylindrocladium), HJ (Aspergillus flavus), CRM (Aspergillus terreus), ABAB (Colletotrichum sp.), and CKT (Aspergillus niger). All isolates were bactericidal against S. epidermidis. Three isolates (HJ, CRM, and CKT) were bactericidal against P. acnes, while PTH and ABAB isolates were bacteriostatic. Tanaman Pegagan (Centella asiatica L.) dikenal luas sebagai obat tradisional dengan berbagai khasiat, termasuk sebagai antibakteri. Penelitian ini bertujuan mengisolasi fungi endofit dari daun Pegagan yang berpotensi menghasilkan metabolit sekunder antibakteri terhadap Staphylococcus epidermidis dan Propionibacterium acnes. Isolasi dilakukan melalui sterilisasi daun dan inokulasi pada media SDA, dilanjutkan fermentasi dan ekstraksi menggunakan etil asetat. Uji aktivitas antibakteri dilakukan dengan metode difusi agar. Diperoleh lima isolat: PTH (Cylindrocladium), HJ (Aspergillus flavus), CRM (Aspergillus terreus), ABAB (Colletotrichum sp.), dan CKT (Aspergillus niger). Seluruh isolat bersifat bakteriosida terhadap S. epidermidis. Tiga isolat (HJ, CRM, dan CKT) bersifat bakteriosida terhadap P. acnes, sedangkan isolat PTH dan ABAB bersifat bakteriostatik.
Isolasi, Identifikasi, dan Uji Aktivitas Antibakteri Fungi Endofit Daun Bidara Arab (Ziziphus spina-christi L.) Terhadap Propionibacterium acnes Dan Staphylococcus epidermidis Salasa, Alfrida Monica; Layuksugi, Defline Apriliana; Pakadang, Sesilia Rante; Ratnah, St.; Asmawati
Media Farmasi Vol 21 No 2 (2025): Media Farmasi Edisi Oktober 2025
Publisher : Jurusan Farmasi Poltekkes kemenkes Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32382/mf.v21i2.605

Abstract

Isolation, Identification, and Antibacterial Activity of Endophytic Fungi from Ziziphus spina-christi Leaves Against Propionibacterium acnes and Staphylococcus epidermidis Ziziphus spina-christi L. (Arabian bidara) has long been used in traditional medicine for preventing acne and enhancing skin health. Acne pathogenesis is closely associated with the proliferation of Propionibacterium acnes and Staphylococcus epidermidis. This study aimed to isolate endophytic fungi from bidara leaves and assess their ability to produce antibacterial secondary metabolites. Endophytes were isolated through surface sterilization and successive purification on Sabouraud Dextrose Agar, followed by fermentation on Potato Dextrose Agar and ethyl acetate extraction. Phytochemical profiling and antibacterial activity were evaluated using standard qualitative assays and the agar diffusion method on Mueller Hinton Agar, respectively.Six pure isolates were obtained: brown (Aspergillus niger), grey (Colletotrichum sp.), cream (Aspergillus terreus), green (Aspergillus flavus), turquoise (Aspergillus fumigatus), and white (Cylindrocladium sp.). Phytochemical screening revealed the presence of alkaloids, flavonoids, polyphenols, and tannins in varying combinations across isolates. Antibacterial testing showed that all isolates inhibited P. acnes and S. epidermidis, except the green isolate, which exhibited no bactericidal activity. These findings indicate that bidara leaf endophytes represent a promising source of antibacterial compounds with potential applications in acne management. Tanaman Bidara Arab (Ziziphus spina-christi L.) adalah tanaman tradisional yang diyakini secara empiris mencegah pertumbuhan jerawat dan menghaluskan kulit. Propionibacterium acnes dan Staphylococcus epidermidis merupakan jenis bakteri yang dapat menimbulkan jerawat. Penelitian ini bertujuan untuk memperoleh isolat fungi endofit Daun Bidara Arab yang berpotensi menghasilkan metabolit sekunder sebagai antibakteri Propinobacterum acnes dan Staphylococcus epidermidis. Fungi endofit diisolasi dengan cara sterilisasi Daun Bidara Arab, selanjutnya diinokulasi berulang pada media Sabouraud Dextrose Agar hingga diperoleh isolat murni. Dilakukan fermentasi pada media Potato Dextrose Agar diekstraksi dengan etil asetat. Skrining fitokimia ekstrak etil asetat dilakukan untuk mendapatkan metabolit sekunder digunakan untuk pengujian antibakteri dengan metode difusi agar pada media Mueller Hinton Agar. Hasil penelitian didapatkan 6 isolat murni, yaitu isolat coklat diduga Aspergillus niger, isolat abu-abu diduga Colletotrichum sp, isolat crem diduga Aspergillus terreus, isolat hijau diduga Aspergillus flavus, isolat toska diduga Aspergillus fumigatus, serta isolat putih diduga Cylindrocladium. Hasil skrining fitokimia menunjukkan isolat coklat mengandung alkaloid, polifenol, tannin, isolat crem mengandung alkaloid, flavonoid, polifenol dan tannin, isolat abu-abu mengandung polifenol dan tannin, isolat putih mengandung flavonoid, polifenol, tannin. Pengujian antibakteri didapatkan semua isolat pada Daun Bidara Arab berpotensi menghambat serta membunuh Propionibacterium acnes dan Staphylococcus epidermidis sedangkan isolat hijau tidak berpotensi dalam membunuh Propionibacterium acnes dan Staphylococcus epidermidis
Co-Authors Adhayanti, Ida Arisanty, Arisanty Asmawati Asmawati Asmawati Ayuasrini, Ayuasrini Cantika, Andi Besse Intan Daswi, Dwi Rachmawaty Dewi Ratnasari Dewi, Sisilia Tresia Rosmala Hiyara, Miftahul Karim, Djuniasti Karim, Djuniasti Karim Layuksugi, Defline Apriliana Lisda, Lisda Novita Andriani, Novita Novita Novita Ratnah, St. Ratnah, St. Ratnah Rosmala Dewi, Sisilia Teresia Salasa, Alfrida Monica Suhardi, Jessica Altin Sukmawaty, Muli