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All Journal HAYATI Journal of Biosciences BIOMA : Jurnal Biologi Makassar Jurnal Kesehatan Medika Saintika JURNAL KESEHATAN PERINTIS JOURNAL OF KOMODO SCIENCE EDUCATION Community Development Journal: Jurnal Pengabdian Masyarakat pendidikan, science, teknologi, dan ekonomi
Anggun Sophia
UNIVERSITAS PERINTIS INDONESIA
Agriculture, Biological Sciences & Forestry Humanities Earth & Planetary Sciences Economics, Econometrics & Finance Education Health Professions Medicine & Pharmacology Nursing Public Health Other

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Journal : JURNAL KESEHATAN PERINTIS

 1 
Ekstrak Daun Jeruk Purut (Citrus hystrix D.C) Mampu Menghambat Pertumbuhan Candida albicans Anggun Sophia; Suraini Suraini; Mahmud Wahyu Pangestu
JURNAL KESEHATAN PERINTIS Vol 8 No 2 (2021): Jurnal Kesehatan Perintis
Publisher : LPPM UNIVERSITAS PERINTIS INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33653/jkp.v8i2.643

Abstract

Ekstrak daun jeruk purut mengandung beberapa komponen aktif seperti saponin, polifenol, flavonoid dan tanin yang dapat menghambat pertumbuhan jamut. Tujuan penelitian ini untuk menguji ekstrak daun jeruk purut sebagai senyawa penghambat pertumbuhan Candida albicans dan memperoleh konsentrasi ekstrak daun jeruk purut paling baik dalam menghambat pertumbuhan Candida albicans. Manfaat penelitian ini untuk menguji ekstrak daun jeruk purut dan konsentrasi yang paling baik dalam menghambat pertumbuhan Candida albicans. Penelitian ini menggunakan penelitian ekperimental laboratorium dengan rancangan acak lengkap dengan berbagai konsentrasi ekstrak 10%, 20%, 40% 80% sebagai perlakuan dan dibandingkan dengan kontrol positif ketokonazol serta kontrol negatif Carboxymethyl cellulose (CMC) dengan 6 pengulangan. Hasil pengukuran diameter zona hambat Candida albicans di uji menggunakan uji statistic One way Anova (Analysis of variance) selanjutnya dilakukan uji duncan’s pada taraf 5%. Analisis terhadap hasil penelitian mengidentifikasi ekstrak daun jeruk purut mampu memberikan penghambatan terhadap Candida albicans. Penghambatan pertumbuhan terbaik ditunjukkan pada perlakuan ekstrak 80% dengan rerata diameter koloni 3.69±0.14 cm dan ketokonazol sebagai kontrol positif 4.40 cm. Dengan demikian, ekstrak daun jeruk purut berpotensi untuk menghambat pertumbuhan Candida albicans.
Deteksi Gen Jamur Candida albicans pada Saliva Penderita Diabetes Melitus Dengan Metode Polymerase Chain Reaction Sophia, Anggun; Suraini, Suraini; Arhesta, Silvi
JURNAL KESEHATAN PERINTIS Vol 11 No 2 (2024): Jurnal Kesehatan Perintis
Publisher : LPPM UNIVERSITAS PERINTIS INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33653/jkp.v11i2.1101

Abstract

Diabetes mellitus is a predisposing factor that increases the risk of oral candidiasis, where the oral cavity of patients becomes an ideal environment for fungal growth due to excess glucose in saliva secretion. This glucose accumulation, along with disturbances in the salivary glands, can trigger the growth of Candida albicans, making it a significant health issue. To accurately detect the presence of Candida albicans, the Polymerase Chain Reaction (PCR) method is used, which allows for the identification of fungal genes with high sensitivity. The aim of this study is to detect the Candida albicans gene in the saliva of women with diabetes mellitus using the Polymerase Chain Reaction method. This research is an analytical study with a cross-sectional design. The samples used in this study are saliva from 10 diabetes mellitus patients experiencing oral pain symptoms. The examination procedures include saliva sample collection, fungal culture testing using Sabouraud Dextrose Agar (SDA), conventional identification, and fungal gene detection using the PCR method. This process includes fungal sample preparation, DNA extraction, DNA amplification, and agarose gel electrophoresis. The results show that 1 respondent tested positive for Candida albicans in the saliva sample, with a percentage of 10%, while 90% were negative. Positive test results were determined through macroscopic analysis of fungal cultures, microscopic examination with Gram staining, and further testing using the germ tube method. The positive sample was then analyzed for fungal gene detection using the PCR method with Internal Transcribed Spacer (ITS), where Candida albicans was detected in the ITS region with a product length of 600 bp.
Deteksi Gen Jamur Candida albicans pada Saliva Penderita Diabetes Melitus Dengan Metode Polymerase Chain Reaction Sophia, Anggun; Suraini, Suraini; Arhesta, Silvi
JURNAL KESEHATAN PERINTIS Vol. 11 No. 2 (2024): Jurnal Kesehatan Perintis
Publisher : LPPM UNIVERSITAS PERINTIS INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33653/jkp.v11i2.1101

Abstract

Diabetes mellitus is a predisposing factor that increases the risk of oral candidiasis, where the oral cavity of patients becomes an ideal environment for fungal growth due to excess glucose in saliva secretion. This glucose accumulation, along with disturbances in the salivary glands, can trigger the growth of Candida albicans, making it a significant health issue. To accurately detect the presence of Candida albicans, the Polymerase Chain Reaction (PCR) method is used, which allows for the identification of fungal genes with high sensitivity. The aim of this study is to detect the Candida albicans gene in the saliva of women with diabetes mellitus using the Polymerase Chain Reaction method. This research is an analytical study with a cross-sectional design. The samples used in this study are saliva from 10 diabetes mellitus patients experiencing oral pain symptoms. The examination procedures include saliva sample collection, fungal culture testing using Sabouraud Dextrose Agar (SDA), conventional identification, and fungal gene detection using the PCR method. This process includes fungal sample preparation, DNA extraction, DNA amplification, and agarose gel electrophoresis. The results show that 1 respondent tested positive for Candida albicans in the saliva sample, with a percentage of 10%, while 90% were negative. Positive test results were determined through macroscopic analysis of fungal cultures, microscopic examination with Gram staining, and further testing using the germ tube method. The positive sample was then analyzed for fungal gene detection using the PCR method with Internal Transcribed Spacer (ITS), where Candida albicans was detected in the ITS region with a product length of 600 bp.
Jamur Endofit Aspergillus spp. dari Ceriops tagal sebagai Kandidat Penghasil Antibiotika Abrar, Mohammad; Agustien, Anthoni; Maliza, Rita; Sophia, Anggun
JURNAL KESEHATAN PERINTIS Vol. 12 No. 2 (2025): Jurnal Kesehatan Perintis
Publisher : LPPM UNIVERSITAS PERINTIS INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33653/m6xjng91

Abstract

Tumbuhan mangrove Ceriops tagal diketahui sebagai salah satu sumber jamur endofitik yang mampu menghasilkan senyawa metabolit bioaktif dengan berbagai aktivitas biologis, termasuk potensi sebagai antibiotik untuk mengatasi masalah resistensi antimikroba yang terus meningkat secara global. Penelitian ini bertujuan untuk mengisolasi jamur endofitik Aspergillus spp. dari daun, akar, dan ranting C. tagal, serta mengevaluasi potensinya sebagai penghasil senyawa antibiotik terhadap Staphylococcus aureus, Escherichia coli, dan Candida albicans. Isolat dikultivasi pada suhu ruang selama 7 hari dalam kondisi statis, kemudian metabolit sekundernya diuji menggunakan metode difusi cakram (Kirby-Bauer). Sebanyak delapan isolat Aspergillus spp. berhasil diperoleh, yaitu AS7, AS8, AS12, AS26, AS47, AS51, AS53, dan AS85, yang menunjukkan variasi morfologi koloni serta potensi antibakteri. Hasil uji menunjukkan sebagian besar isolat mampu menghambat pertumbuhan E. coli dan S. aureus, tetapi tidak menunjukkan aktivitas terhadap C. albicans. Aktivitas terhadap E. coli umumnya lemah, kecuali AS8 (10,45 mm) yang tergolong kuat. Aktivitas terhadap S. aureus lebih tinggi, dengan tiga isolat AS7 (11,89 mm), AS12 (11,45 mm), dan AS51 (10,92 mm) masuk dalam kategori kuat. Dari delapan isolat yang diperoleh, AS7, AS8, AS12, dan AS51 menunjukkan aktivitas kuat terhadap Staphylococcus aureus dan Escherichia coli, namun tidak menunjukkan aktivitas terhadap Candida albicans. Temuan ini menegaskan potensi jamur endofitik mangrove sebagai sumber kandidat antibiotik baru.
Co-Authors -, Suraini Abrar, Mohammad Anthoni Agustien Arhesta, Silvi Chairul Fadhlan, Adli Hendra Kusuma Karimi, Kasman Mahmud Wahyu Pangestu Mairawita, Mairawita Maliza, Rita Mildawati Mildawati Niken Niken Pertiwi, Vera Rita Permatasari Ritonga, Muhammad Azli RR. Ella Evrita Hestiandari Solfiyeni Solfiyeni Sri Indrayati Suraini, Suraini Susanto, Vetra Vera Pujani