Garuda - Garba Rujukan Digital

Deteksi Gen Jamur Candida albicans pada Saliva Penderita Diabetes Melitus Dengan Metode Polymerase Chain Reaction Sophia, Anggun; Suraini, Suraini; Arhesta, Silvi
JURNAL KESEHATAN PERINTIS Vol 11 No 2 (2024): Jurnal Kesehatan Perintis
Publisher : LPPM UNIVERSITAS PERINTIS INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33653/jkp.v11i2.1101

Diabetes mellitus is a predisposing factor that increases the risk of oral candidiasis, where the oral cavity of patients becomes an ideal environment for fungal growth due to excess glucose in saliva secretion. This glucose accumulation, along with disturbances in the salivary glands, can trigger the growth of Candida albicans, making it a significant health issue. To accurately detect the presence of Candida albicans, the Polymerase Chain Reaction (PCR) method is used, which allows for the identification of fungal genes with high sensitivity. The aim of this study is to detect the Candida albicans gene in the saliva of women with diabetes mellitus using the Polymerase Chain Reaction method. This research is an analytical study with a cross-sectional design. The samples used in this study are saliva from 10 diabetes mellitus patients experiencing oral pain symptoms. The examination procedures include saliva sample collection, fungal culture testing using Sabouraud Dextrose Agar (SDA), conventional identification, and fungal gene detection using the PCR method. This process includes fungal sample preparation, DNA extraction, DNA amplification, and agarose gel electrophoresis. The results show that 1 respondent tested positive for Candida albicans in the saliva sample, with a percentage of 10%, while 90% were negative. Positive test results were determined through macroscopic analysis of fungal cultures, microscopic examination with Gram staining, and further testing using the germ tube method. The positive sample was then analyzed for fungal gene detection using the PCR method with Internal Transcribed Spacer (ITS), where Candida albicans was detected in the ITS region with a product length of 600 bp.

Species Diversity of Genus Aspergillus, Endophytic Fungal Isolated From Mangrove Ceriops tagal and their Antibiotic Potential Sophia, Anggun; Agustien, Anthoni; Chairul; Syamsuardi
HAYATI Journal of Biosciences Vol. 32 No. 5 (2025): September 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.5.1174-1184

The rapid increase in antimicrobial resistance has encouraged development of new natural and urgent strategies to fight drug-resistant pathogens, one of which is using endophytic fungi. Endophytic fungi found in Ceriops tagal mangrove plant, which can adapt to extreme salinity environments of up to 60 ppt. Endophytic fungi are isolated from leaves, branch, and roots. Each isolate was identified morphologically and cultured in potato dextrose broth media for 21 days. After incubation, liquid culture was extracted with ethyl acetate and evaporated to obtain a thick extract. The Kirby Bauer method was used to evaluate the antibiotic potential of each endophytic fungal extract against pathogenic microbes Staphylococcus aureus, Escherichia coli, and Candida albicans. Concentration used was 5%, antibiotics used as positive controls were chloramphenicol (30μg/disk), nystatin (100 IU/disk), as negative controls DMSO. Three endophytic fungi were isolated from roots (ECT7, ECT 8, ECT 10), two from leaves (ECT 26, ECT 28) and six from branches (ECT 31, ECT 47, ECT 48, ECT 51, ECT 55 and ECT 85). Fungal isolates ECT10 has been proven to effectively exhibit strong antibiotic activity against microorganisms E. coli, S. aureus, and C. albicans, with inhibition zones measuring 13.0 mm, 11.8 mm, and 11.6 mm, respectively.

Aktivitas Antijamur Kulit Singkong terhadap Candida albicans dari Saliva Penderita Diabetes yang Diidentifikasi Molekuler Sophia, Anggun; Suraini, Suraini; Niken, Niken
Jurnal Kesehatan Medika Saintika Vol 16, No 1 (2025): Juni 2025
Publisher : Stikes Syedza Saintika Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30633/jkms.v16i1.3218

Infeksi jamur oportunistik seperti Candida albicans meningkat pada penderita diabetes melitus akibat kondisi hiperglikemia yang mendukung pertumbuhan jamur di rongga mulut. Penelitian ini bertujuan untuk mengetahui aktivitas antijamur ekstrak kulit singkong (Manihot esculenta) terhadap Candida albicans yang diisolasi dari saliva penderita diabetes melitus dan diidentifikasi secara molekuler menggunakan metode PCR. Isolat diperoleh melalui kultur saliva, lalu diidentifikasi secara konvensional (makroskopis, mikroskopis, germ tube) dan molekuler dengan hasil amplifikasi gen ITS menunjukkan pita DNA ±600 bp. Ekstrak kulit singkong dibuat melalui metode maserasi menggunakan etanol 96% dan diuji aktivitasnya dengan metode difusi cakram pada konsentrasi 20%, 40%, 60%, 70%, dan 80%. Hasil menunjukkan bahwa konsentrasi 80% memiliki daya hambat paling tinggi terhadap pertumbuhan Candida albicans dengan rata-rata diameter zona hambat 2.46 ± 0.31 cm (kategori sangat kuat). Uji statistik ANOVA menunjukkan perbedaan signifikan antar perlakuan (p < 0.05). Kesimpulannya, ekstrak kulit singkong memiliki potensi sebagai agen antijamur alami terhadap Candida albicans yang diisolasi dari penderita diabetes, terutama pada konsentrasi tinggi.Kata kunci : Antijamur; kulit singkong; Candida albicans;diabetes melitus; molekuler.

DIVERSIFIKASI PRODUK BERBASIS TALAS UNTUK PENINGKATAN EKONOMI DESA TUAPEJAT MENTAWAI, SUMATRA BARAT Solfiyeni, Solfiyeni; Mairawita, Mairawita; Mildawati, Mildawati; Pertiwi, Vera; Fadhlan, Adli; Ritonga, Muhammad Azli; Kusuma, Hendra; Sophia, Anggun; Karimi, Kasman; Pujani, Vera
Community Development Journal : Jurnal Pengabdian Masyarakat Vol. 6 No. 2 (2025): Volume 6 No. 2 Tahun 2025
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/cdj.v6i2.41699

Desa Tuapejat adalah sebuah desa yang terletak di kepulauan Mentawai dengan hasil budidaya masyarakat salahsatunya adalah talas. Talas merupakan tanaman lokal yang melimpah di wilayah ini, namun pemanfaatannya masih terbatas pada konsumsi tradisional, dan bahkan talas kadang hanya dijadikan sebagai makanan ternak di wilayah tersebut. Untuk meningkatkan nilai perekonomian masyarakat maka telah dilakukan kegiatan pelatihan pengolahan umbi talas menjadi berbagai macam makanan di desa Tuapejat. Kegiatan diikuti oleh peserta mitra dengan latar belakang ibu rumah tangga dan anggota PKK desa Tuapejat. Kegiatan pengabdian kepada masyarakat ini bertujuan untuk meningkatkan pengetahuan dan keterampilan masyarakat dalam pengolahan umbi talas, meningkatkan diversifikasi produk olahan umbi talas untuk menciptakan nilai tambah dan untuk meningkatkan pendapatan masyarakat melalui pemasaran produk olahan talas. Program ini melibatkan sosialisasi dan praktik pembuatan berbagai olahan talas, seperti keripik talas dengan aneka rasa, nugget talas, cake talas, bola-bola talas isi cokelat, dodol talas cokelat, dan keripik talas balado. Peserta mitra diajarkan bagaimana pemilihan talas yang baik, cara mengupas, pengolahan dan pengemasan produk yang sudah jadi. Hasil dari kegiatan ini menunjukkan bahwa para peserta, terutama ibu-ibu, mulai memproduksi dan menjual olahan berbasis talas secara kelompok maupun mandiri. Tim selalu memberikan pendampingan terhadap peserta mitra untuk memantau dan memberikan penyuluhan agar peserta mitra dapat meningkatkan produksi dan penjualannya. Kegiatan ini berkontribusi pada peningkatan keterampilan peserta serta membuka peluang usaha baru yang dapat meningkatkan pendapatan keluarga.

Deteksi Gen Jamur Candida albicans pada Saliva Penderita Diabetes Melitus Dengan Metode Polymerase Chain Reaction Sophia, Anggun; Suraini, Suraini; Arhesta, Silvi
JURNAL KESEHATAN PERINTIS Vol. 11 No. 2 (2024): Jurnal Kesehatan Perintis
Publisher : LPPM UNIVERSITAS PERINTIS INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33653/jkp.v11i2.1101

Diabetes mellitus is a predisposing factor that increases the risk of oral candidiasis, where the oral cavity of patients becomes an ideal environment for fungal growth due to excess glucose in saliva secretion. This glucose accumulation, along with disturbances in the salivary glands, can trigger the growth of Candida albicans, making it a significant health issue. To accurately detect the presence of Candida albicans, the Polymerase Chain Reaction (PCR) method is used, which allows for the identification of fungal genes with high sensitivity. The aim of this study is to detect the Candida albicans gene in the saliva of women with diabetes mellitus using the Polymerase Chain Reaction method. This research is an analytical study with a cross-sectional design. The samples used in this study are saliva from 10 diabetes mellitus patients experiencing oral pain symptoms. The examination procedures include saliva sample collection, fungal culture testing using Sabouraud Dextrose Agar (SDA), conventional identification, and fungal gene detection using the PCR method. This process includes fungal sample preparation, DNA extraction, DNA amplification, and agarose gel electrophoresis. The results show that 1 respondent tested positive for Candida albicans in the saliva sample, with a percentage of 10%, while 90% were negative. Positive test results were determined through macroscopic analysis of fungal cultures, microscopic examination with Gram staining, and further testing using the germ tube method. The positive sample was then analyzed for fungal gene detection using the PCR method with Internal Transcribed Spacer (ITS), where Candida albicans was detected in the ITS region with a product length of 600 bp.

Title

Found 15 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 15 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 15 Documents
Search