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Utilization of Rhizoctonia Mycorrhizae for Orchid Late Blight Control in Sustainable Agricultural Soelistijono, R.; Daryanti, Daryanti; Rakhmawati, Dian; Rianto, Prisma Aditya; Utomo, Herry
Biosaintifika: Journal of Biology & Biology Education Vol. 16 No. 1 (2024): April 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v15i1.3498

Abstract

Vanda tricolor is currently a protected species because is the occurrence of orchid late blight caused by the Fusarium sp. pathogenic fungus. The objective of this study was to investigate the effectiveness of Rhizoctonia mycorrhizae in inducing resistance against Fusarium sp., the causative agent of late blight, in V. tricolor. The research employed CRBD with three treatments and six replications. Rhizoctonia mycorrhizae were isolated and identified following the procedure outlined by Bayman. Pre-inoculation of Rhizoctonia mycorrhizae was conducted on PDA media, and mycorrhizae were subsequently inoculated onto the roots of V. tricolor seedlings derived from tissue culture. The presence of peloton structures on the roots was determined using the Nakano methods. The Saravanan method was employed to measure peroxidase activity. The results demonstrated that V. tricolor induced by Rhizoctonia mycorrhizae exhibited resistance against Fusarium sp. and displayed the formation of peroxidase enzymes, peloton structures, and lignification. In contrast, the non-induced orchids suffered significant damage to the epidermal tissue of the leaves, did not exhibit an increase in peroxidase enzymes, and failed to form peloton or lignified structures. The novelty of this research is the use of Rhizoctonia mycorrhizae to control orchid late blight diseases at Mount Merapi.
Biofortification in Palm Oil Based on Saponin Nanotechnology: Innovation in Nutritional Stability, Antioxidants and for Functional Quality Sapto Priyadi; H. Haryuni; R. Soelistijono; Siti Mardhika Sari
Agricultural Science Vol. 8 No. 1 (2024): September
Publisher : Faculty of Agriculture, Merdeka University Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55173/agriscience.v8i1.152

Abstract

Palm oil is the world's main vegetable oil commodity and significantly contributes to the food industry. However, challenges related to the low content of certain nutrients and susceptibility to lipid oxidation are major issues that affect product quality. Fortification technology is an innovative solution to improve palm oil's nutritional value and oxidative stability. This article aims to evaluate the development of modern technology in palm oil fortification, including the addition of vitamin A, vitamin E, bioactive compounds, and the use of natural and synthetic antioxidants. Various methods, such as microencapsulation, nanotechnology, and biofortification techniques, are discussed in the context of their effectiveness in maintaining nutritional stability, preventing lipid oxidation, and extending product shelf life. This review highlights the challenges of implementing fortification technology, including technical and regulatory aspects in the food industry. The results of the review indicate that fortification technology improves nutritional quality and the oxidative stability of palm oil, thus supporting the development of healthier and more value-added food products. The implementation of vitamin A and E fortification with nanotechnology is expected to be able to answer global nutritional challenges and increase the competitiveness of the palm oil industry in the international market. 
Mikoriza Rhizoctonia : Sebagai Agen Ketahanan Pada Anggrek Dendrobium Soelistijono, R.; Supriyadi, Teguh; Tiara Maharani, Annissa; Imani Fatihah, Syalaysa
JURNAL ILMIAH AGRINECA Vol. 25 No. 1 (2025): JURNAL ILMIAH AGRINECA
Publisher : Fakultas Pertanian, Universitas Tunas Pembangunan Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36728/afp.v25i1.4612

Abstract

Drought and low rainfall are natural phenomena that occurred in 2022-2023. These factors (abiotic factors) caused diseases in orchids, which affect the metabolism of growth and the flowering process, making the plants unable to survive in their habitat because they cannot reproduce, thus classifying them under Appendix 2 (CITES). However, some orchid species that are endemic are able to withstand drought and low rainfall. It is suspected that the orchids' ability to endure these factors is due to the role of endophytic fungi (Rhizoctonia binucleate / BNR), which can associate with orchids and produce secondary metabolites that help supply nutrients to the orchids during drought stress. This study aims to evaluate the biological resistance of different strains of Rhizoctonia binucleate / BNR isolates in Dendrobium orchids to understand their impact on water stress. The research method used a completely randomized design with a single stage consisting of two factors and five replications. Factor 1: orchid seedling types consisting of S1 = Phalaenopsis violaceae (epiphytic orchid) and S2 = Dendrobium aggregatum (epiphytic orchid). Factor 2: types of Rhizoctonia mycorrhiza application consisting of K0 = control, K1 = Rhizoctonia mycorrhiza application.
The Analysis of Architectural YOLOv5 Convolutional Neural Networks for Detecting Apple Leaf Diseases Erkamim, Moh.; Subarkah, Muhammad Zidni; Soelistijono, R.
Journal of Applied Agricultural Science and Technology Vol. 9 No. 1 (2025): Journal of Applied Agricultural Science and Technology
Publisher : Green Engineering Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55043/jaast.v9i1.251

Abstract

Apple cultivation is crucial to agricultural economies, particularly in regions with sub-tropical climates, such as Indonesia, where apple farming is expanding rapidly. However, managing diseases and pests is essential for maintaining optimal crop yields, as they can significantly reduce production. Among the common diseases affecting apple trees are Scab, Black Rot, and Cedar Apple Rust, which primarily impact leaves and threaten the total health of the plant. Therefore, this research aimed to develop an effective model for detecting apple leaf diseases using the architectural YOLOv5 Convolutional Neural Networks (CNNs). The analysis was conducted between November 2022 and February 2023 at the Smart City Information System (SIKC) laboratory, including 120 apple leaf samples collected from Tawangmangu. Additionally, secondary data containing 30 images for each disease category, consisting of Healthy, Scab, Black Rot, and Cedar Apple Rust, were used as a benchmark. The performance of YOLOv5 was evaluated based on several metrics, including Precision, Recall, mAP@0.5, and mAP@0.5:0.95. The results showed that Cedar Apple Rust was the most prevalent disease identified among the samples. YOLOv5 performed exceptionally well in detecting disease symptoms, achieving a Precision score of 0.810, Recall of 0.981, mAP@0.5 of 0.950, and mAP@0.5:0.95 of 0.765 on the test dataset. These results showed that the proposed model was highly accurate and reliable for the early detection of apple leaf diseases, offering significant potential for improving disease management strategies and increasing the efficiency of apple production.
Respon Bawang Merah Varietas Bima Terhadap Aplikasi Mikoriza dan Pupuk Organik Cair Limbah Rumah Tangga: Respon Bawang Merah Varietas Bima Terhadap Aplikasi Mikoriza dan Pupuk Organik Cair Limbah Rumah Tangga Daryanti; Soelistijono, R.; Mardhika Sari, Siti; Soemarah Kurnia Dewi, Tyas; Restu Putra Abadi, Pramudika
JURNAL ILMIAH AGRINECA Vol 25 No 2 (2025): JURNAL ILMIAH AGRINECA
Publisher : Fakultas Pertanian, Universitas Tunas Pembangunan Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36728/afp.v25i2.5387

Abstract

Shallots are a horticultural commodity whose demand is always increasing. Organic cultivation by utilizing household waste as organic fertilizer and mycorrhizae is one way to increase the production of Bima variety shallots. The study was conducted from February to May 2025 in Koripan Village, Matesih District, Karanganyar Regency. This study aimed to determine the effect of mycorrhizae and liquid organic fertilizer (POC) from household waste on the growth and yield of Bima variety shallots. The study was conducted using a Completely Randomized Block Design (RAKL) using two factors, namely the mycorrhizae dose (0, 5, and 10 grams) and the POC watering interval (0, 3, 6, and 9 days). The results showed that the administration of 10 grams of mycorrhizae gave the best results in terms of bulb weight (32.75 grams), number of bulbs (7.20), and consumption bulb weight (31.12 grams). Meanwhile, the three-day POC watering interval (P2) resulted in the highest tuber weight (36.83 grams), tuber number (8.00 grams), and tuber consumption weight (35.44 grams) of all treatments. The combination of mycorrhizal treatment and POC watering interval significantly increased plant productivity in an organic and environmentally friendly manner.
PRODUKSI PESTISIDA NABATI KULIT BAWANG PUTIH DAN DAUN PEPAYA DI KELOMPOK TANI PEMUDA TANGGUH Haryuni, Haryuni; Suprapti, Endang; Soelistijono, R.; Aziez, Achmad Fatchul; Priyadi, Sapto; Prasetio, Suji Tegar
GANESHA: Jurnal Pengabdian Masyarakat Vol 5 No 2 (2025): Juli 2025
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Tunas Pembangunan Surakarta (UTP)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36728/ganesha.v5i2.4796

Abstract

Salah satu program pemerintah dalam menjaga stabilitas pangan nasional adalah melalui penguatan ketahanan pangan yang ramah lingkungan dan berkelanjutan, dengan mengedepankan pemanfaatan bahan-bahan alami serta mengurangi ketergantungan terhadap pestisida kimia sintetis. Kelompok Tani Pemuda Tangguh menghadapi kendala meningkatnya serangan Organisme Pengganggu Tanaman (OPT), yang berdampak pada penurunan produktivitas dan kualitas hasil panen. Kegiatan Program Kemitraan Masyarakat (PKM) ini bertujuan untuk memberdayakan petani agar mampu memanfaatkan limbah rumah tangga dan tanaman pekarangan, khususnya kulit bawang putih, daun pepaya, dan lidah buaya sebagai bahan dasar pembuatan pestisida nabati (pesnab) dalam rangka pengendalian hama dan penyakit tanaman secara alami. Metode kegiatan meliputi: 1) sosialisasi mengenai pentingnya pengendalian hama berbasis hayati, 2) pelatihan pembuatan dan formulasi pestisida nabati dari bahan lokal, 3) evaluasi efektivitas dan monitoring penggunaan pesnab di lahan pertanian, serta 4) pendampingan teknis kepada petani dalam aplikasi lapangan. Kegiatan ini berhasil meningkatkan pengetahuan dan keterampilan petani dalam memproduksi dan mengaplikasikan pestisida nabati secara mandiri. Indikator keberhasilan kegiatan ditunjukkan melalui pencapaian target monitoring dan evaluasi, serta respon positif petani terhadap efektivitas pesnab dalam menekan serangan OPT. Program ini mendukung upaya pertanian berkelanjutan berbasis kearifan lokal dan pemanfaatan sumber daya hayati yang melimpah di lingkungan sekitar.
PEMBERDAYAAN PEMUDA DESA MELALUI BIOEKONOMI PERTANIAN: STRATEGI PEMBANGUNAN KEMANDIRIAN DAN KETAHANAN PANGAN LOKAL Priyadi, Sapto Priyadi; Soelistijono, R.; Haryuni , Haryuni; Wibowo, Maulana Tulus; Sholkhan, Ahmad
GANESHA: Jurnal Pengabdian Masyarakat Vol 5 No 2 (2025): Juli 2025
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Tunas Pembangunan Surakarta (UTP)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36728/ganesha.v5i2.5200

Abstract

Permasalahan rendahnya pengetahuan pemuda desa dalam penerapan sistem pertanian berkelanjutan serta pengelolaan limbah pangan sektor on-farm menjadi tantangan utama dalam pembangunan kemandirian ekonomi dan ketahanan pangan lokal. Sebagian besar limbah pangan hanya dibuang atau dibakar, menyebabkan kerusakan lingkungan dan hilangnya potensi sumber daya hayati yang bernilai. Keterbatasan akses terhadap pelatihan teknis, minimnya dukungan modal, serta belum optimalnya alokasi dana desa untuk program produktif menambah kompleksitas masalah ini. Program pengabdian masyarakat ini bertujuan untuk memberdayakan pemuda desa melalui pendekatan bioekonomi pertanian dengan memanfaatkan limbah pangan sebagai bahan dasar media tanam hidroponik. Metode yang diterapkan meliputi edukasi partisipatif, pelatihan teknis pembuatan media tanam substrat dari limbah pangan, serta pendampingan budidaya hidroponik. Hasil analisis laboratorium menunjukkan bahwa pupuk organik cair (POC) dari limbah pangan memiliki kandungan N total 1,69%, P?O? 1,14%, K?O 1,25%, dan C-organik 15,31%, yang layak digunakan sebagai pupuk (sumber nutrisi) pada budidaya hidroponik rumah tangga untuk sayuran daun. Pemuda yang terlibat menunjukkan peningkatan pengetahuan, keterampilan teknis pembuatan POC, dan minat berwirausaha berbasis pertanian berkelanjutan. Kesimpulan, integrasi bioekonomi dan teknologi sederhana dalam pengelolaan limbah pangan terbukti efektif dalam membangun kapasitas pemuda dan memperkuat ketahanan pangan lokal berbasis sumber daya desa.