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Marsuni, Yusriadi
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Immunology & microbiology Languange, Linguistic, Communication & Media Social Sciences Other

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Journal : JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA

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Molecular identification of fungi and the types of toxins produced from contaminated corn grain in Satui, Tanah Bumbu, South Kalimantan, Indonesia Salamiah, Salamiah; Mariana, Mariana; Marsuni, Yusriadi; Pramudi Indar, Muhammad; Sepe, Muslimin; Aphrodyanti, Lyswiana; Abbas, Saipul
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 25 No. 2 (2025): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLAN
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.225337-349

Abstract

Fungal contamination in stored corn grain not only reduces grain quality but alsoposes risks to animal and human health due to mycotoxin production. This study highlights the importance of early detection and identification of fungal pathogens in corn as a key aspect of plant protection and postharvest management, as well as the need to determine the types and concentrations of toxins produced. Corn samples were collected from a storage warehouse in Satui Village, Kota Baru Regency, South Kalimantan. Fungal isolation was conducted at the Phytopathology Laboratory, Department of Plant Pests and Diseases, Faculty of Agriculture, Universitas Lambung Mangkurat. PCR analysis and gene sequencing were performed at the Genetics Sciences Laboratory, Jakarta, while toxin type and content analyses were carried out at the Animal Husbandry Laboratory, Universitas Gadjah Mada, Yogyakarta. From 11 microbial isolates obtained from corn grain, only one fungal species was identified, namely Aspergillus flavus. This species was found to produce 8.00 ppb of aflatoxin, which remains below the established safety thresholds of 15 ppb for B1 and 20 ppb for total aflatoxins.
Co-Authors Abdul Azis Ahmad MAULANA Akhmad Rizali Aprilliana, Noor Badaruddin Badaruddin Bersinar, Saras Dewi Fitriyanti, Dewi Emilya, Riska Farah, Nurin Nisa Fathur Rahman Fikasari, Devaliana catria Firdaus, Ruly Hariyadi, Nursyifa Nada Helda Orbani Rosa Ismed Setya Budi Khamidah, Noor Krisnawati, Wiwik Lyswiana Aphrodyanti Magfirah, Asmaul Mariana Mariana Muhammad Indar Pramudi Muhammad Naufal, Muhammad Muhammad Redho Noorkomalasari, Noorkomalasari Nurdi, Mi’rajzul Hudia Pramudi Indar, Muhammad Rahmatullah Rahmatullah Rifani, Muhammad Indar Rifani, Muhammad Indra Rina Lestari Saipul Abbas Salamiah . Samharinto Samharinto Sari, Yulia Padma Saupi, Muhammad Sepe, Muslimin Setiawan, Mario Yudi Tarigan, Divayona Begidir Br Tarigan Yanti, Dewi Dhea Yuliana, Maria Yuliani, Renita Anti