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All Journal Medical Laboratory Analysis and Sciences Journal Borneo Journal of Medical Laboratory Technology Journal of Community Service and Empowerment Prisma Sains: Jurnal Pengkajian Ilmu dan Pembelajaran Matematika dan IPA IKIP Mataram JUSTER: Jurnal Sains dan Terapan Journal for Quality in Public Health Edubiotik : Jurnal Pendidikan, Biologi dan Terapan

Chalies Diah Pratiwi

Unknown Affiliation

Author-ID : 1511006

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Other

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Identifikasi Sedimen Urine pada Penduduk yang Mengkonsumsi Air Sumur di Desa Besole Kecamatan Besuki Kabupaten Tulungagung Chalies Diah Pratiwi; Eka Puspitasari
Borneo Journal of Medical Laboratory Technology Vol 1 No 2 (2019): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Drinking water that is consumed should be clean and healthy. To avoid illness and disruption of bodily functions such as kidney function, liver, brain, teeth, and even mental disorders. Some people in Besole village still use dug well water as a source of drinking water. The geographical state of the town of Besole, Tulungagung district, is a mountainous area of marble containing lime which allows for mineral content, main calcium in the drinking water of residents. This study aims to determine the identification of urine sediments in residents who consume well water in Besole Village, Besuki District, Tulungagung Regency in 2018. This type of research is descriptive non-analytic, by determining the percentage of urine sediments that consume well water through an examination at the Pathology Laboratory STIKes Hutama Abdi Husada Tulungagung. The number of samples is 30 people, with simple random sampling technique. Based on the research conducted found the results of abnormal urine sediment percentage, ie, 6.7% leukocytes, 3.3% erythrocytes, 16.7% epithelium, 30% calcium oxalate and 3.3% bacteria. Water containing lime should be boiled, precipitated and filtered before consumption.

Deskripsi Jumlah Leukosit dan Laju Endap Darah pada Pasien Tuberkulosis di Rumah Sakit Paru Dungus Madiun Chalies Diah Pratiwi; Eka Puspitasari; Vivi Nurohmah
Borneo Journal of Medical Laboratory Technology Vol 2 No 1 (2019): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Tuberculosis is an infectious disease of global concern caused of bacterium Mycobacterium tuberculosis. It can attack lung, liver, kidneys, bones or skin. If a person infected by tuberculosis, the body will begin the mechanism of inflammation as a form of haematological manifestation. The purpose of this study was to describe the number of leukocytes and Blood Sedimentation Rate (BSR) in tuberculosis patients. The research design used is non-analytic descriptive. The population in this study were all tuberculosis patients at the Dungus Madiun Lung Hospital in 2018Using the Quota Sampling technique, the sample in this study was 30 respondents. This research was conducted on May 2018. The results of the study on tuberculosis patients showed that the number of leukocytes in TB patients was 9120 cells/µL blood, 11 (37%) patients had leukocytosis and 19 (63%) sufferers have a nominal leukocyte count. The lowest leukocyte count was 4100 cells/µL of blood and the highest was 24700 cells/µL of blood. The average value of BSR in TB patients was 71 mm/hour I, 29 (96.6%) patients experienced an increase in BSR values and 1 (3.4%) patients had normal BSR values. The lowest value of BSR is 11 mm/hour I and the highest value of BSR is 106 mm/hour I. Prevention of tuberculosis infection can be done by conducting laboratory diagnosis to find out how far the disease goes and choose the right OAT for patients. With intensive treatment, it will reduce the number of leukocytes and reduce the risk for MDR TB

Blood calcium levels in factory workers who consume supplements drinks Chalies Diah Pratiwi; Hariyanto Hariyanto; Amelia Rahma Perdana Putri
Medical Laboratory Analysis and Sciences Journal Vol 1 No 1 (2019): May 2019
Publisher : Department of D3 Medical Technology Laboratory STIKes Hutama Abdi Husada Tulungagung

Energy supplement drinks are drinks that contain caffeine, taurine, sweeteners, and other substances that have the effect of increasing energy, concentration, alertness, maintaining physical strength, and reducing drowsiness. Blood calcium is an important mineral that the body needs. The purpose of this study was to determine blood calcium levels in marble factory workers in Besole Village, Tulungagung Regency. The research design used was Analytical Descriptive. The population in this study were all marble factory workers who routinely consumed energy supplement drinks in the village of Besole in 2018 using a total sampling technique of 30 respondents. This research was conducted at the Laboratory of Dr. Iskak Tulungagung in May 2018 using the O-CPC method. The results obtained from the examination of blood calcium in marble factory workers showed 10 respondents (33%) had blood calcium levels within normal limits, 17 respondents (57%) had deep blood calcium levels. low limit and 3 respondents (10%) had very low blood calcium levels. Consumption of energy supplement drinks that exceed the threshold can damage the kidneys and affect blood calcium levels because the caffeine content in energy supplements can increase the release of calcium in the bones so that more calcium is released through the urine.

The total plate count of beras kencur herbal medicine in sub-district market Tulungagung city Eka Puspitasari; Chalies Diah Pratiwi; Tri Wahyuni
Medical Laboratory Analysis and Sciences Journal Vol 1 No 2 (2019): November 2019
Publisher : Department of D3 Medical Technology Laboratory STIKes Hutama Abdi Husada Tulungagung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35584/melysa.v1i2.30

Herbal medicine is a traditional drink made from natural ingredients for human health and is made with methods and equipment that are easier in its simplicity which often does not pay attention to hygiene factors and can be a medium for disease transmission. Based on the Regulation of the Head of the Republic of Indonesia Drug and Food Control Agency No. 12 of 2014 concerning requirements for drug fluids in the form of microbial contamination / ALT ≤104 colonies/ml. The purpose of this study was to study the estimated numbers of traditional herbs sold in the Tulungagung District market. The research design used is descriptive. The sampling technique uses a total sampling technique of 6 samples from 4 different markets. Sample meeting with the ALT method in May 2018 in the microbiology laboratory of STIKes Hutama Abdi Husada Tulungagung. The results of the study found that three out of six samples of traditional herbal medicine of kencur rice exceeded the microbial contamination threshold with an average yield of 1 x 107 CFU / ml. The study concludes is that 50% of the sample is not suitable for consumption and 50% of the sample is suitable for consumption. This requires Regulation of BPOM RI Number 12 the year 2014 Regarding Quality Requirements for Traditional Medicines, namely ≤104 colonies/ml. Herbal traders need to pay attention to raw materials, processing, equipment, air used, and containers used.

PERBEDAAN KADAR HEMOGLOBIN PADA PASIEN SEBELUM DAN SESUDAH OPERASI CAESAR Nosa Ika Cahyariza; Hariyanto Hariyanto; Chalies Diah Pratiwi
Medical Laboratory Analysis and Sciences Journal Vol 2 No 2 (2020): November 2020
Publisher : Department of D3 Medical Technology Laboratory STIKes Hutama Abdi Husada Tulungagung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35584/melysa.v2i2.54

Caesarean section is a process to remove a fetus or baby through an incision in the abdominal wall and upper uterus because a normal birth process is not possible. The process by Caesarean section requires careful preparation because the result can be bleeding. One of the tests before and after Caesarean section is checking the hemoglobin level. The purpose of this study was to determine the differences in hemoglobin levels before and after Caesarean section. This research is a quantitative analysis. Sampling was conducted in April 2018 at RSIA Fauziah Tulungagung and hemoglobin levels were checked using the Cyanmehemoglobin method at the STIKes Hutama Abdi Husada Tulungagung Pathology Laboratory. The population of this study were all pregnant women who underwent a Caesarean section at RSIA Fauziah Tulungagung using a total sampling technique of 30 samples. The instrument used was a photometer, then the data was processed using SPSS 16 software and analyzed using the Paired T-Test technique. The results of research conducted on 30 samples of Caesarean section patients obtained an average hemoglobin level before Caesarean section 13.13 g / dl and an average hemoglobin level after Caesarean section 12.06 g / dl after the Paired T-Test was obtained. 0.002 <0.05. It can be concluded that there is a significant difference between the hemoglobin levels before and after Caesarean section. Bleeding during Caesarean section causes hemoglobin levels in pregnant women to decrease.

Pengaruh Serum yang Disimpan Selama Lima Hari Suhu 2-8°C dengan Serum yang Diperiksa Langsung pada Pemeriksaan Kolesterol Total: The Effect of Serum Stored for Five Days Temperature of 2-8°C with Serum That is Checked Directly on Total Cholesterol Testing Chalies Diah Pratiwi; Hariyanto Hariyanto; Andyanita Hanif Hermawati; Intan Niawati Fajrin
Borneo Journal of Medical Laboratory Technology Vol. 4 No. 2 (2022): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v4i2.3790

Examination of blood cholesterol at this time became the trend of laboratory examination that much in demand by most people because of many diseases caused by hypercholesterolemia due to wrong diet or high cholesterol levels. Damaged equipment, reagent exhaustion, a large number of samples, laboratory distance with the sampling site too far, and power outages resulted in delayed laboratory examination that required sample storage. This study aimed to determine the effect of serum storage for five days with a serum examined directly to examine total cholesterol. The research design used was an experiment. The population in this research is all female students; Level 3 Study Program D3 Health Analyst STIKes Hutama Abdi Husada Tulungagung, using the Purposive sampling technique, counted 15 respondents. This research was conducted in May 2018. The data obtained were processed using SPSS 16 software and analyzed by Paired t-Test. This study showed the average value of total cholesterol levels in serum examined directly at 163 mg/dl while stored for five days temperature of 2-8 ° C at 158 mg/dl. After the paired test obtained value of P-value = 0.000, so P-value = 0.000 <α = 0.05, it can be concluded there is an influence between total cholesterol levels are checked directly and stored for five days temperature 2-8°C. Five days of serum storage affects the results of total cholesterol tests that lead to a decrease in total cholesterol, but the reduction is insignificant.

AN ANALYSIS OF PREDISPOSING, ENABLING, AND REINFORCING FACTORS IN PREVENTING THE TRANSMISSION OF PULMONARY TUBERCULOSIS DISEASE ON PATIENTS’ RELATIVES IN TULUNGAGUNG REGENCY Chalies Diah Pratiwi; Sandu Siyoto; Indasah Indasah
Journal for Quality in Public Health Vol. 1 No. 1 (2017): Journal for Quality in Public Health
Publisher : Master of Public Health Program Institut Ilmu Kesehatan STRADA Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30994/jqph.v1i1.6

Tuberculosis (TB) is one of the major causes of death. In the report of Tuberculosis Global 2014 which was realeased by World Health Organization (WHO) stated that the incidence happened in Indonesia was 1 million new cases every year. The percentage of the cases in Indonesia is 10 percent from entire cases in the world, so as to be the most second cases country along with Tiongkok. The objective of this study is to know the effect of predisposing, enabling, and reinforcing factors towards the efforts in preventing the transmission of pulmonary TB diseases on patients’ relatives in Tulungagung regency.This study was non experimental quantitative research by using research design with Cross Sectional observation method. The samples this study were the relatives who have the most emotional closeness with the patient of pulmonary TB as much as 285 respondents. The data obtained through questionnaire then it was analyzed by using ordinal regression test. Based on the ordinal regression test which was conducted where ρ < 0,05, hence that there was the effect of gender on the prevention of pulmonary TB transmission in Tulungagung regency, the effect of educational level on the prevention of pulmonary TB transmission in Tulungagung regency, the effect of knowledge on the prevention of Pulmonary TB transmission in Tulungagung regency, the effect of dwelling density on the prevention of pulmonary TB transmission in Tulungagung regency and the effect of coaching officer on the prevention of Pulmonary TB transmission in Tulungagung regency. Improvement of guidance and quality of service in Pulmonary TB prevention program by involving the family is expected so the patient will do prevention effort to pulmonary tuberculosis disease to support local goverment effort in decreasing incidence of pulmonary TB generally and particularly maintain personal health to achieve health society which play an active role in national development.

The Analysis of SGOT, SGPT, and ALP in Chili Farmers Exposed to Pesticides in Kendalbulur Village, Tulungagung Pratiwi, Chalies Diah; Hermawati, Andyanita Hanif; Cahyariza, Nosa Ika
Prisma Sains : Jurnal Pengkajian Ilmu dan Pembelajaran Matematika dan IPA IKIP Mataram Vol 13, No 1: January 2025
Publisher : IKIP Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/j-ps.v13i1.10014

Chili farmers in Kendalbulur Village are the main subjects that have a great influence due to exposure to pesticide residues (toxicity). Pesticide toxicity affects enzymes produced by the liver that cause changes in its working system. This study aims to look at the characteristics and levels of the enzymes SGOT, SGPT, and ALP in chili farmers exposed to pesticides that perform spraying activities. The SGOT-SGPT liver function test is a health screening that combines the examination of SGOT and SGPT enzyme levels in the blood. The purpose of this liver function test is to detect hepatitis or cirrhosis of the liver in chili farmers. The alkaline phosphatase (ALP) enzyme test is conducted to help diagnose and evaluate cirrhosis and inflammatory diseases of the liver. In addition to evaluating liver function, the alkaline phosphatase (ALP) enzyme test can also help detect bone disorders or protein deficiencies in chili farmers. Respondents were 20 chili farmers and pesticide users with an age range of 34–71 years. Test method using the non-analytical descriptive method and sampling technique using total sampling. The examination carried out is the rate of SGOT, SGPT, and ALP using spectrophotometry. The results of the SGOT examination showed that all respondents had SGOT levels in the normal range of <40 U/L, where the lowest result was 16 U/L and the highest was 35 U/L. While SGPT showed that all respondents were in the normal range of 40 U/L, with the lowest result of 2 U/L and the highest of 23 U/L, the ALP levels of many respondents were above normal values. The lowest value is 131 U/L, and the highest is 222 U/L. The results showed that the respondents showed disruption of the liver because ALP levels give high results. Where should ALP levels decrease with age.

Perbedaan Penggunaan Antikoagulan K3EDTA Konvensional dan Vacutainer Terhadap Kadar Indeks Eritrosit Menggunakan Hematology Analyzer Fu'ana, Yan; Ulya, Nala Fidarotul; Pratiwi, Chalies Diah; Ningrum, Dyah Setyowati
Borneo Journal of Medical Laboratory Technology Vol. 7 No. 2 (2025): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v7i2.9414

Antikoagulan adalah zat yang digunakan untuk mencegah terjadinya pembekuan pada sampel darah. Pada pemeriksaan hematologi yang sering digunakan adalah antikoagulan K3EDTA. Keunggulan K3EDTA adalah tidak mempengaruhi morfologi dari komponen darah. Ketersediaan EDTA ada 2 bentuk yaitu konvensional (dengan melarutkan EDTA dari bentuk serbuknya) dan vacutainer (EDTA yang sudah siap pakai di tabung vacutainer ungu). Penggunaan 2 bentuk EDTA tersebut memiliki kelebihan dan kekurangn masing – masing. Penelitian ini bertujuan untuk mengetahui perbedaan penggunaan antikoagulan K3EDTA konvensional dan vacutainer terhadap kadar indeks eritrosit menggunakan hematology analyzer. Analisis data menggunakan uji T Independen. Setelah dilakukan pengujian, diperoleh p>0.05 yang artinya tidak terdapat perbedaan. Berdasarkan hasil tersebut dapat disimpulkan bahwa tidak terdapat perbedaan penggunaan antikoagulan K3EDTA konvensional dan vacutainer terhadap kadar indeks eritrosit menggunakan hematology analyzer.

Antibacterial actifity of kecombrang (Etlingera elatior) leaf ethanol extract to against Klebsiella pneumoniae Puspitasari, Eka; Diah Pratiwi, Chalies; Rival Novian, Dede; Dayu Rahma Turista, Dora
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Vol. 7 No. 01 (2022): Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Publisher : Biology Education Department, Universitas Insan Budi Utomo, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33503/ebio.v7i01.1867

Infectious diseases caused by bacteria in generals can be cured using antibacterial. The use of antibacterials tends to cause local hypersensitivity either on the skin or mucous membranes so that their use is starting to be reduced. Therefore, the development of natural antibacterials made from plants to reduce resistance is very necessary. kecombrang (Etlingera elatior) is one of the plants that has been shown to have activity in inhibiting bacterial growth. This research is an experimental study that aims to determine the effectiveness of the ethanol extract of the leaves of kecombrang in inhibiting the growth of Klebsiella pneumoniae bacteria in vitro. The method used is the well method with a completely randomized design. The sample came from the Klebsiella pneumoniae bacteria culture. Kecombrang leaf ethanol extract was made in concentrations of 20%, 40%, 60%, 80% and 100%. The extract was tested onKlebsiella pneumoniae bacteria and observed for the formation of inhibition zones. Inhibition the results of the zone measuremens were then analyzed descriptively. The results showed kecombrang leaf ethanol extract 20%, 40%, 60%, 80%, and 100% produce inhibition zone diameter 1.9 mm; 2.6 mm; 7.7 mm; 12.1 mm; and 14.7 mm, 0.4 mm for the negative control and positive control 22.0 mm. Concentration 100% has better antibacterial ability due to higher compound content. The test results show the class of secondary metaboliteskecombrang leaf ethanol extract contains flavonoids, tannin, and saponin. The conclusion of this study that the ethanol extract of kecombrang leaves

Co-Authors Amelia Rahma Perdana Putri Andyanita Hanif Hermawati Cahyariza, Nosa Ika Dayu Rahma Turista, Dora Eka Puspitasari Eka Puspitasari Fu'ana, Yan Fu’ana , Yan Hariyanto Hariyanto Hariyanto Hariyanto Indasah Intan Niawati Fajrin Nala Fidarotul Ulya Ningrum, Dyah Setyowati Nosa Ika Cahyariza Nosa Ika Cahyariza Nosa Ika Cahyariza Novian, Dede Rival Rival Novian, Dede Sandu Siyoto Tri Wahyuni Turista, Dora Dayu Rahma Vivi Nurohmah Yan Fu’ana

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