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All Journal Jurnal Teknologi Laboratorium THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Jurnal Analis Kesehatan Medical Laboratory Analysis and Sciences Journal Borneo Journal of Medical Laboratory Technology Journal for Quality in Public Health Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Genbinesia Journal of Biology Jurnal Pendidikan: Teori, Penelitian, dan Pengembangan
Eka Puspitasari
STIKes Hutama Abdi Husada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Other

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The Growth of Staphylococcus aureus in the blood agar plate media of sheep blood and human blood groups A, B, AB, and O Turista, Dora Dayu Rahma; Puspitasari, Eka
Jurnal Teknologi Laboratorium Vol 8 No 1 (2019): 2019 (1)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (479.651 KB) | DOI: 10.29238/teknolabjournal.v8i1.155

Abstract

BAP media is a medium used to distinguish pathogenic bacteria based on their hemolytic power on red blood cells. Staphyllococcus aureus is a bacterium that is able to emolate red blood cells with 3 types of hemolysis, namely α, β, γ, and δ. Usually BAP media is made by adding 5-10% sheep blood. Making BAP media using sheep blood has become a problem for several laboratories today, including health education laboratories. This is because the health education laboratory does not yet have a sheep farm, so it has not been able to procure sheep blood. The use of human blood as a substitute for sheep blood in making BAP media may be a solution, but it is not yet known whether there are differences in the growth and hemolysis of S. aureus bacteria on BAP media in sheep's blood and human blood. This research is an experimental study with a completely randomized design (CRD) of 3 replications which aims to determine whether there are differences in growth and hemolysis of bacteria S. aureus in BAP media of sheep blood and human blood groups A, B, AB, and O. The results showed that S. aureus bacteria could grow and show hemolysis in BAP media in sheep blood and human blood in groups A, B, AB, and O. The results of subsequent studies analyzed ANOVA using the software spss for windows with a significant level of 0.05. From the results of research and data analysis it can be concluded that S. aureus bacteria can grow and show hemolysis in BAP media of sheep blood and human blood groups A, B, AB and O, but there are significant differences in the number of S. aureus bacteria colonies grown in BAP media of sheep's blood and human blood groups A, B, AB and O.
Human immune response to SARS-CoV-2 infection Wina Nurtias, Lia Yosaneri; Rahma Turista, Dora Dayu; Puspitasari, Eka
Jurnal Teknologi Laboratorium Vol 9 No 1 (2020): 2020 (1): Special Edition "COVID-19"
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (961.717 KB) | DOI: 10.29238/teknolabjournal.v9i1.223

Abstract

COVID-19 is an acute respiratory infection caused by a new type of Coronavirus, SARS-CoV-2, which first appeared in Wuhan, China in December 2019. COVID-19 then became a pandemic in various countries in early 2020. In this article it contains review that discusses the immune response in humans due to SARS-CoV-2 infection, using the narrative literature review method, a total of 36 articles (6 from Elsevier, 24 from PMC, and 6 from Springer). It is known that the pathogenesis of COVID-19 and the manufacture of drugs and vaccines are still under investigation, but in infected patients, innate immune responses in the form of alveolar macrophages, dendritic cells, airway epithelial cells, congenital lymphocytes, and neutrophils work together in the fight against infection. Next comes the adaptive immune response in the form of antibodies (immunoglobulins) which help in fighting infections due to SARS-CoV-2. These immune responses include increasing levels of cytokines, coagulation parameters, C-reactive protein, neutrophils, and decreasing total lymphocytes. It is also known that COVID-19 patients with severe disease often experience higher total antibody, IgM responses, and IgG responses than COVID-19 patients without congenital disease. IgG antibodies are present in the serum, so the serum in COVID-19 patients who have recovered can be used for therapy in COVID-19 patients who have not healed, as long as the drug and vaccine are under investigation.
UJI ANTIFUNGI KETEPENG CINA (Cassia alata L.) TERHADAP Trichophyton rubrum DAN Candida albicans Qurrotu A'yunin Lathifah; Eka Puspitasari; Dora Dayu Rahma Turista
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 4, No 1 (2021): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v4i1.7362

Abstract

Ketepeng Cina merupakan salah satu tumbuhan liar di Indonesia yang berpotensi sebagai antijamur. Pengujian antijamur dilakukan dengan cara difusi cakram menggunakan ekstrak dengan konsentrasi 20%, 40%, 60%, dan 80%. Hasil pengujian menunjukkan zona hambat pada semua konsentrasi ekstrak dan zona terluas dihasilkan pada konsentrasi 90%.
The incidence of onychomycosis in farmers in Krajan Hamlet, Beji Village, Boyolangu District, Tulungagung Regency Eka Puspitasari
Medical Laboratory Analysis and Sciences Journal Vol 4 No 2 (2022): November 2022
Publisher : Department of D3 Medical Technology Laboratory STIKes Hutama Abdi Husada Tulungagung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35584/melysa.v4i2.67

Abstract

Onychomycosis is a nail disorder caused by fungal infections, both by dermatophytes, non-dermatophytes, and yeast with symptoms of damage to the nails, including the nails become thicker, cracked, uneven, not shiny, and there is a change in nail color to white, yellow, brown, to black. Onychomycosis can occur in farmers due to working directly in wet, damp and dirty places. This study aims to determine the incidence of onychomycosis in farmers in krajan Hamlet, Beji Village, Boyolangu District, Tulungagung Regency. This study is a descriptive study with Purposive Sampling sampling techniques and samples as many as 15 farmers who meet the criteria, examination of fungi using culture and microscopic methods on fungal colonies. The results showed that all respondents farmers infected with onychomycosis (100%) with 9 species of fungi found Trichophyton rubrum (21.43%), Rhizopus sp (21.43%), Penicillium sp (14.30%), Trichophyton mentagrophytes (10.71%), Trichophyton tonsurans (10.71%), Aspergillus niger (7.14%), Mucor sp (7.14%), Aspergillus flavus (3.57%), and Microsporum canis (3.57%). The presence of the fungus is due to the habit of farmers who do not wear personal protective equipment, do not regularly wash their hands and feet using soap, and pay less attention to nail hygiene. The conclusion of this study the incidence of onychomycosis in Farmers in Hamlet inKrajan Village Beji District Boyolangu Tulungagung Regency is very high as many as 15 people (100%). It is expected that farmers pay attention to nail hygiene, wash their hands and feet with soap, and wear personal protective equipment while working.
ANALYSIS OF THE FACTORS AFFECTING THE QUALITY OF THE CHEMICAL AND MICROBIOLOGICAL DRINKING WATER AT THE DEPOT DRINKING WATER REFILL TULUNGAGUNG DISTRICT Eka Puspitasari
Journal for Quality in Public Health Vol. 1 No. 1 (2017): Journal for Quality in Public Health
Publisher : Master of Public Health Program Institut Ilmu Kesehatan STRADA Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30994/jqph.v1i1.10

Abstract

Refill drinking water Depot is an industry that does the processing of the raw water into drinking water and sell directly to the consumer. Mn metal and bacterial Coliform is an indicator of refill drinking water polluters. This research aims to find the factors influencing the quality of Microbiology and chemistry at the depot drinking water drinking water refill Tulungagung district. Research using survey method with Cross sectional approach is analytical. The sampling technique used was Simple Random Sampling with a sample of 89 depot drinking water refill. Technique of data analysis using Regression and Logistic regression test linear. Independent variable examined was the source of raw water, the depot operator hygiene of drinking water, hygiene, sanitation, which covers three aspects namely sanitary location, building and facility sanitation. The results showed from 89 depot examined known as much as 79.8% sanitation facilities are not qualified, the value of the MPN 59.55% of eligible and levels of Mn 100% qualified. From Regresilogistik test results between independent variable with the value of the MPN indicate significance of 0.008 < (α = 0.05) on sanitation facilities are variable. This means there are influences between facilities sanitation microbiological quality of drinking water. On Linear regression test results between independent variable with the value obtained significance levels of Mn 0.47 > ((α = 0.05) which means no influence between independent variables with the chemical quality of drinking water. The lack of sanitation facilities increases the potential for bacterial contamination through the operator. Therefore the availability of on-site sanitation depot drinking water is a major factor that affects the quality of drinking water microbiology refills.
Daya Antibakteri Ketepeng Cina (Cassia alata L.) Terhadap Staphylococcus aureus, Pseudomonas aerugenosa, dan Klebsiella pneumonia Lathifah, Qurrotu A’yunin; Turista, Dora Dayu Rahma; Puspitasari, Eka
Jurnal Analis Kesehatan Vol. 10 No. 1 (2021): JURNAL ANALIS KESEHATAN
Publisher : Department of Health Analyst, Politeknik Kesehatan, Kementerian Kesehatan Tanjungkarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26630/jak.v10i1.2718

Abstract

Penyakit kulit akibat infeksi banyak dijumpai di Indonesia dan menjadi masalah besar di bidang kesehatan. Masyarakat telah menggunakan daun ketepeng cina (Cassia alata L.) sebagai obat penyakit kulit. Tujuan penelitian ini untuk mengetahui aktivitas dan efektivitas antibakteri ekstrak daun ketepeng cina terhadap bakteri Staphylococcus aureus, Pseudomonas aerogenosa, dan Klebsiella pneumonia. Metode uji yang digunakan yaitu difusi disk (Kirby Bauer). Sampel yang digunakan berupa ekstrak etanol daun ketepeng cina konsentrasi 10%, 30%, 50%, 70%, dan 90%. Hasil penelitian ini menunjukkan adanya zona hambat di area sekitar disk yang berisi ekstrak. Diameter zona hambat bakteri gram negatif dalam penelitian ini relatif lebih besar dibanding gram positif. Hal ini dikarenakan ekstrak yang digunakan dalam penelitian agak encer. Kesimpulan yang diperoleh dalam penelitian ini ekstrak daun ketepeng cina memiliki aktivitas antibakteri terhadap bakteri Staphylococcus aureus, Pseudomonas aeruginosa dan Klebsiella pneumonia. Konsentrasi ekstrak yang paling efektif menghambat pertumbuhan bakteri Staphylococcus aureus adalah 70%, dan konsentrasi 90% merupakan konsentrasi yang paling efektif dalam menghambat pertumbuhan bakteri Pseudomonas aeruginosa, serta Klebsiella pneumonia.
Antibacterial actifity of kecombrang (Etlingera elatior) leaf ethanol extract to against Klebsiella pneumoniae Puspitasari, Eka; Diah Pratiwi, Chalies; Rival Novian, Dede; Dayu Rahma Turista, Dora
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Vol. 7 No. 01 (2022): Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Publisher : Biology Education Department, Universitas Insan Budi Utomo, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33503/ebio.v7i01.1867

Abstract

Infectious diseases caused by bacteria in generals can be cured using antibacterial. The use of antibacterials tends to cause local hypersensitivity either on the skin or mucous membranes so that their use is starting to be reduced. Therefore, the development of natural antibacterials made from plants to reduce resistance is very necessary. kecombrang (Etlingera elatior) is one of the plants that has been shown to have activity in inhibiting bacterial growth. This research is an experimental study that aims to determine the effectiveness of the ethanol extract of the leaves of kecombrang in inhibiting the growth of Klebsiella pneumoniae bacteria in vitro. The method used is the well method with a completely randomized design. The sample came from the Klebsiella pneumoniae bacteria culture. Kecombrang leaf ethanol extract was made in concentrations of 20%, 40%, 60%, 80% and 100%. The extract was tested onKlebsiella pneumoniae bacteria and observed for the formation of inhibition zones. Inhibition the results of the zone measuremens were then analyzed descriptively. The results showed kecombrang leaf ethanol extract 20%, 40%, 60%, 80%, and 100% produce inhibition zone diameter 1.9 mm; 2.6 mm; 7.7 mm; 12.1 mm; and 14.7 mm, 0.4 mm for the negative control and positive control 22.0 mm. Concentration 100% has better antibacterial ability due to higher compound content. The test results show the class of secondary metaboliteskecombrang leaf ethanol extract contains flavonoids, tannin, and saponin. The conclusion of this study that the ethanol extract of kecombrang leaves
Medicinal importance of Cassia alata L. (Fabaceae): A comprehensive review Turista, Dora Dayu Rahma; Lathifah, Qurrotu A’yunin; Puspitasari, Eka; Murtadlo, Ahmad Affan Ali; Aini, Nur Sofiatul
Genbinesia Journal of Biology Vol. 3 No. 2 (2024): March 2024
Publisher : Generasi Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55655/genbinesia.v3i2.68

Abstract

Cassia alata included in the Fabaceae family which spread in tropical and humid areas. All part of the C. alata contains phytochemical so that C. alata is potential medicinal plants. Leaves extract was reported to produce flavonoids, alkaloid, tannin, and cynogenic glycoside. Stems extract contains alkaloid, flavonoid, saponins, oxalate, pheno, and tannin. Roots extract contains alkaloid, saponins, flavonoids, tannins, and phenols. Flower contains saponin, tannin, anthraquinones, flavonoid, glicoside, steroid, and volatile oil. C. alata has been reported to be antiinflamatory, antioxidant, antibacterial, antifungal, antiviral, antiparasitic, antitumor, anticancer, antidiabetic, hepatoprotective, and have a laxative effect. The aim of this study is to give a sneak peek view on C. alata’s taxonomy, distribution, phytochemical, pharmacological activities, and the toxicological effects.
Analysis of cholesterol and triglyceride levels in obese patients Pratiwi, Chalies Diah; Puspitasari, Eka; Hermawati, Andyanita Hanif
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Vol. 8 No. 01 (2023): Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Publisher : Biology Education Department, Universitas Insan Budi Utomo, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33503/ebio.v8i01.1061

Abstract

Obesity is excessive fat accumulation in the body. Continuous obesity and excessive food intake can cause metabolic system disorders in the form of hypercholesterolemia. Analysis of cholesterol and triglyceride levels in obese patients needs to be carried out to assist them in making healthier lifestyle changes. This study aims to analyze cholesterol and triglyceride levels in obese patients. The research method used is experimental with a laboratory approach. The samples to be analyzed were 20 obese people obtained by purposive sampling technique in the Tulungagung area. The research instruments used were height and weight check sheets and cholesterol and triglyceride test results sheets. Research data analysis technique by examining cholesterol and triglyceride levels using a MicroLab C-300 spectrophotometer and will be analyzed descriptively quantitatively. The results showed that 15 patients had normal cholesterol levels (75%) with criteria <200 mg/dl and 5 patients had high cholesterol levels (25%) with criteria >200 mg/dl, while there were 17 patients with normal triglyceride levels (85 %) with criteria <150 mg/dl and 3 patients had high triglyceride levels (15%) with criteria >150 mg/dl. The conclusion of this study is that people who are obese do not necessarily have high cholesterol and triglyceride levels. However, when a person weighs > 80 kg, it is recommended to start paying attention to a healthier lifestyle.
Pemeriksaan Kadar Hemoglobin pada Penderita Pediculosis di Pondok Pesantren Raudlotul Musthofa Rejotangan Tulungagung Kusharyati, Imelda Putri; A’yunin , Qurrotu; Lathifah, Lathifah; Puspitasari, Eka; Fu’ana, Yan
Borneo Journal of Medical Laboratory Technology Vol. 8 No. 2 (2026): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v8i2.10726

Abstract

Pediculosis kapitis merupakan infestasi ektoparasit yang banyak ditemukan di lingkungan padat seperti pondok pesantren. Infestasi berat dapat menimbulkan gatal, gangguan tidur, dan kehilangan darah kronis yang berpotensi menyebabkan anemia. Penelitian ini bertujuan untuk mengetahui gambaran kadar hemoglobin pada santriwati penderita pediculosis di Pondok Pesantren Raudlotul Musthofa Rejotangan Tulungagung. Penelitian menggunakan desain deskriptif dengan pendekatan cross-sectional. Sampel penelitian berjumlah 40 santriwati penderita pediculosis. Pemeriksaan kadar hemoglobin dilakukan menggunakan metode Point of Care Testing (POCT) dengan alat Easy Touch dari darah kapiler. Hasil pemeriksaan menunjukkan 25 responden (62,5%) memiliki kadar hemoglobin normal, 7 responden (17,5%) rendah (anemia), dan 8 responden (20%) tinggi. Infeksi pediculosis dapat memengaruhi kadar hemoglobin pada santriwati. Meskipun sebagian besar responden memiliki kadar hemoglobin normal, masih terdapat 17,5% yang mengalami anemia. Pemeriksaan hemoglobin rutin, edukasi gizi, suplementasi zat besi, serta upaya pencegahan pediculosis penting dilakukan di pesantren untuk menjaga kesehatan santriwati.
Co-Authors Achmad Amirudin, Achmad Aini, Nur Sofiatul Aisiah, Sitti Amaluddin Amaluddin, Amaluddin Andyanita Hanif Hermawati A’yunin , Qurrotu Chalies Diah Pratiwi Dayu Rahma Turista, Dora Fu’ana, Yan Imelda Putri Kusharyati Lathifah, Lathifah Murtadlo, Ahmad Affan Ali Qurrotu A’yunin Lathifah Rival Novian, Dede Turista, Dora Dayu Rahma Wina Nurtias, Lia Yosaneri