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The Growth of Staphylococcus aureus in the blood agar plate media of sheep blood and human blood groups A, B, AB, and O Turista, Dora Dayu Rahma; Puspitasari, Eka
Jurnal Teknologi Laboratorium Vol 8 No 1 (2019): 2019 (1)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (479.651 KB) | DOI: 10.29238/teknolabjournal.v8i1.155

Abstract

BAP media is a medium used to distinguish pathogenic bacteria based on their hemolytic power on red blood cells. Staphyllococcus aureus is a bacterium that is able to emolate red blood cells with 3 types of hemolysis, namely α, β, γ, and δ. Usually BAP media is made by adding 5-10% sheep blood. Making BAP media using sheep blood has become a problem for several laboratories today, including health education laboratories. This is because the health education laboratory does not yet have a sheep farm, so it has not been able to procure sheep blood. The use of human blood as a substitute for sheep blood in making BAP media may be a solution, but it is not yet known whether there are differences in the growth and hemolysis of S. aureus bacteria on BAP media in sheep's blood and human blood. This research is an experimental study with a completely randomized design (CRD) of 3 replications which aims to determine whether there are differences in growth and hemolysis of bacteria S. aureus in BAP media of sheep blood and human blood groups A, B, AB, and O. The results showed that S. aureus bacteria could grow and show hemolysis in BAP media in sheep blood and human blood in groups A, B, AB, and O. The results of subsequent studies analyzed ANOVA using the software spss for windows with a significant level of 0.05. From the results of research and data analysis it can be concluded that S. aureus bacteria can grow and show hemolysis in BAP media of sheep blood and human blood groups A, B, AB and O, but there are significant differences in the number of S. aureus bacteria colonies grown in BAP media of sheep's blood and human blood groups A, B, AB and O.
Human immune response to SARS-CoV-2 infection Wina Nurtias, Lia Yosaneri; Rahma Turista, Dora Dayu; Puspitasari, Eka
Jurnal Teknologi Laboratorium Vol 9 No 1 (2020): 2020 (1): Special Edition "COVID-19"
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (961.717 KB) | DOI: 10.29238/teknolabjournal.v9i1.223

Abstract

COVID-19 is an acute respiratory infection caused by a new type of Coronavirus, SARS-CoV-2, which first appeared in Wuhan, China in December 2019. COVID-19 then became a pandemic in various countries in early 2020. In this article it contains review that discusses the immune response in humans due to SARS-CoV-2 infection, using the narrative literature review method, a total of 36 articles (6 from Elsevier, 24 from PMC, and 6 from Springer). It is known that the pathogenesis of COVID-19 and the manufacture of drugs and vaccines are still under investigation, but in infected patients, innate immune responses in the form of alveolar macrophages, dendritic cells, airway epithelial cells, congenital lymphocytes, and neutrophils work together in the fight against infection. Next comes the adaptive immune response in the form of antibodies (immunoglobulins) which help in fighting infections due to SARS-CoV-2. These immune responses include increasing levels of cytokines, coagulation parameters, C-reactive protein, neutrophils, and decreasing total lymphocytes. It is also known that COVID-19 patients with severe disease often experience higher total antibody, IgM responses, and IgG responses than COVID-19 patients without congenital disease. IgG antibodies are present in the serum, so the serum in COVID-19 patients who have recovered can be used for therapy in COVID-19 patients who have not healed, as long as the drug and vaccine are under investigation.
PENINGKATAN AKTIVITAS DAN HASIL BELAJAR IPS MENGGUNAKAN COURSE REVIEW HORAY DI MIN PONTIANAK TENGGARA Puspitasari, Eka; ., Sugiyono; ., Syamsiati
Jurnal Pendidikan dan Pembelajaran Khatulistiwa Vol 6, No 2 (2017): Februari 2017
Publisher : Jurnal Pendidikan dan Pembelajaran Khatulistiwa

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Abstrak: Penelitian ini bertujuan untuk mendeskripsikan peningkatan aktivitas dan hasil belajar siswa dalam pembelajaran ilmu pengetahuan sosial menggunakan  tipe course review horay di kelas VB MIN Bangka Belitung Pontianak Tenggara. Metode penelitian menggunakan metode deskriptif. Bentuk penelitian adalah penelitian tindakan  kelas yang bersifat kolaboratif. Penelitian dilaksanakan dalam empat siklus yang terdiri dari tahap perencanaan, pelaksanaan, observasi dan refleksi. Teknik pengumpulan data adalah teknik observasi langsung, dan analisis dokumen. Aktivitas belajar siswa menggunakan tipe course review horay siklus I yaitu 71,06%, siklus II 76,56%, siklus III  81,32% dan siklus IV 87,55%, sedangkan hasil belajar menggunakan tipe course review horay baseline yaitu 67,05, siklus I 71,79, siklus II 78,46, siklus III 85,13 dan siklus IV 90,26. Dengan demikian, tipe course review horay dapat meningkatkan aktivitas dan hasil belajar siswa. Kata Kunci: Aktivitas, Hasil, Course Review Horay Absract: The purpose of this research was to describe the improvement of students’ activity and learning outcomes on social sciences learning by using the course reviews horay model to the VB students of MIN Bangka Belitung in South East Pontianak. The method of research was descriptive methods in form of collaborative classroom action research. The Research was carried out in four cycles consist of planning; implementation; observation; and reflection. The data collection technique was direct observation and document analysis. As the result, the students’ activity by using Course Review Horay at the first, second, third, and fourth cycle was 71.06%; 76.56%; 81.32%; and 87.55% respectively. While the learning outcomes by using Course Review Horay baseline was 67.05, with the result at the first, second, third, and fourth cycle was 71.79, 78.46, 85.13, and 90.26 respectively. Thus, the course review horay model can enhance the students’ activity and learning outcomes.   Key Words: Activity, Outcomes, Course Review Horay
Daya Antibakteri Ketepeng Cina (Cassia alata L.) Terhadap Staphylococcus aureus, Pseudomonas aerugenosa, dan Klebsiella pneumonia Lathifah, Qurrotu A’yunin; Turista, Dora Dayu Rahma; Puspitasari, Eka
Jurnal Analis Kesehatan Vol. 10 No. 1 (2021): JURNAL ANALIS KESEHATAN
Publisher : Department of Health Analyst, Politeknik Kesehatan, Kementerian Kesehatan Tanjungkarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26630/jak.v10i1.2718

Abstract

Penyakit kulit akibat infeksi banyak dijumpai di Indonesia dan menjadi masalah besar di bidang kesehatan. Masyarakat telah menggunakan daun ketepeng cina (Cassia alata L.) sebagai obat penyakit kulit. Tujuan penelitian ini untuk mengetahui aktivitas dan efektivitas antibakteri ekstrak daun ketepeng cina terhadap bakteri Staphylococcus aureus, Pseudomonas aerogenosa, dan Klebsiella pneumonia. Metode uji yang digunakan yaitu difusi disk (Kirby Bauer). Sampel yang digunakan berupa ekstrak etanol daun ketepeng cina konsentrasi 10%, 30%, 50%, 70%, dan 90%. Hasil penelitian ini menunjukkan adanya zona hambat di area sekitar disk yang berisi ekstrak. Diameter zona hambat bakteri gram negatif dalam penelitian ini relatif lebih besar dibanding gram positif. Hal ini dikarenakan ekstrak yang digunakan dalam penelitian agak encer. Kesimpulan yang diperoleh dalam penelitian ini ekstrak daun ketepeng cina memiliki aktivitas antibakteri terhadap bakteri Staphylococcus aureus, Pseudomonas aeruginosa dan Klebsiella pneumonia. Konsentrasi ekstrak yang paling efektif menghambat pertumbuhan bakteri Staphylococcus aureus adalah 70%, dan konsentrasi 90% merupakan konsentrasi yang paling efektif dalam menghambat pertumbuhan bakteri Pseudomonas aeruginosa, serta Klebsiella pneumonia.
Penggunaan Mozaik dalam Meningkatkan Motorik Halus Anak di TK Pertiwi 1 Kota Bengkulu Sari, Liza Permata; Puspitasari, Eka; Suryadi, Didik
TRIADIK Vol. 22 No. 1: April 2023
Publisher : UNIB Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33369/triadik.v22i1.33539

Abstract

Abstrak Perkembangan motorik halus menjadi salah satu aspek penting yang perlu dikembangkan pada anak usia dini karena perkembangan motorik halus sangat dibutuhkan oleh anak agar anak dapat tumbuh dan berkembang secara optimal. Ada 4 alasan pentingnya pengembangan motorik halus pada anak yaitu sosial, akademis, pekerjaan dan psikologis. Penelitian ini bertujuan untuk meningkatkan kemampuan motoric halus anak usia dini melalui mozaik. Jenis penelitian yang digunakan adalah Penelitian Tindakan Kelas (PTK) atau Classroom Action Research (CAR) yang dilakukan di TK Pertiwi 1 Kota Bengkulu. Populasi penelitian ini adalah 15 anak dengan sampel penelitian yaitu anak kelas B1. Instrumen pengumpulan data pada yang digunakan oleh peneliti adalah lembar observasi. Analisis data yang digunakan adalah analisis data kuantitatif. Hasil penelitian menunjukkan bahwa setelah kegiatan mozaik diberikan terjadi peningkatan pada kemampuan motorik halus anak Hal ini dapat dilihat dari hasil observasi setiap pertemuan yang menunjukkan adanya peningkatan pada sertiap siklus dalam pengembangan motorik halus melalui mozaik. Pada siklus I pertemuan 1 didapatkan hasil 20% Pertemuan 2 siklus I memperoleh hasil 33% dan pada pertemuan 3 mendapatkan hasil 40%. Pada siklus II pertemuan 1 diperoleh hasil 60%. Siklus II pertemuan 1 didapatkan hasil 73% dan pertemuan 3 mendapatkan hasil 87%. Berdasarkan data diaras dapat disimpulkan bahwa melalui mozaik dapat meningkatkan motorik halus anak.
Volunteering as a Companion for Foreign Student Exchange in Muhammadiyah University of Parepare Arsyad, Ainun; Puspitasari, Eka; Ammade, Salasiah
BABASAL English Education Journal Vol. 4 No. 2 (2023)
Publisher : English Education Study Program

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32529/beej.v4i2.2695

Abstract

In light of globalization in the twenty-first century, graduates need to interact with other cultures to be successful in the international labor market. The author argues that universities need to consider their social relevance in contemporary complex societies. This is a Companion for Foreign Student Exchange in Muhammadiyah University of Parepare.Collaborative learning in cultural exchange: Exploring its current trend and feature. This research method is qualitative, namely taking references from books or from articles that these works for many foreign students face challenges in adjusting to life in their new country. To assist foreign students in overcoming these challenges, volunteering as a companion by applying the STAR (Situation, Challenge, Action, Reflection) method. Volunteering as a co-host on foreign exchange students from Philippines University using the STAR method provides an effective framework for providing support, overcoming challenges, and encouraging reflection. By understanding the situation, identifying challenges, taking appropriate action, and encouraging reflection, volunteer companions provide a holistic and purposeful approach to helping foreign students succeed during their exchange.
Scabies education and detection at Raudlatul Musthofa junior high school Puspitasari, Eka; Lathifah, Qurrotu A’yuunin; Hariyanto, H.; Fu'ana, Yan
Journal of Community Service and Empowerment Vol. 4 No. 3 (2023): December
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/jcse.v4i3.28243

Abstract

Scabies is a skin disease endemic in tropical and subtropical climates that is highly contagious and generally affects groups of people with low socioeconomic conditions, poor personal hygiene and sanitation. This activity aimed to make efforts to prevent, provide education, and assist public health development so that it is expected to improve public health in boarding schools students.  This study aims to make efforts to prevent, provide education, and assist public health development so that it is expected to improve public health in boarding schools. The sample to be analyzed was 19 students from Raudlatul Junior High School. Examination of parasites that cause scabies is done with a skin swab. The results of observations that have been made of Raudlatul Musthofa Junior High School students show that the majority of students have carried out personal hygine well, namely all 19 students (100%) bathing at least twice a day using soap, keeping hands and feet clean, and changing clothes when dirty. A total of 14 students (74%) do not exchange personal items such as towels, do not take turns in clothes, and do not dry clothes in the room. A total of 15 students (79%) cleaned the bed, 18 students (95%) did not lend toiletries such as soap, and 16 students (84%) did not experience symptoms of frequent itching at night, rashes (spots), wounds, or dry and crusty skin. The results of skin swab examinations on 19 students at Raudlatul Musthofa seen under a microscope obtained as many as 13 students (68%) samples contained bacteria and as many as 6 students (32%) samples contained fungi.
USING VIDEO ANIMATION IN CONTEXTUAL TEACHING AND LEARNING TO ENHANCE STUDENT ENGAGEMENT IN SCIENCE LEARNING Puspitasari, Eka; Krismanto, Wawan; Tuken, Ritha; Pasinggi, Yonathan S.
QUANTUM: Jurnal Inovasi Pendidikan Sains Vol 15, No 1 (2024): April 2024
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/quantum.v15i1.17134

Abstract

Science learning that only prioritizes conceptual learning and ignores relevance to the environmental context and society in which students live needs to be immediately abandoned and changed to contextual learning oriented towards student activity and engagement. The research aims to implement the contextual teaching and learning (CTL) model assisted by video animation to improve student learning processes and engagement in science learning about the Water Cycle. For this reason, a qualitative approach to this type of classroom action research is a research method that is considered relevant. Class actions were carried out in cycles (planning, implementation, observation and reflection) in class 5 of SDN 3 Watang Sidenreng, Sidrap Regency, South Sulawesi. Three instruments were used as data collection tools: teacher and student observation sheets and student learning engagement observation. Descriptive analysis was used for the analysis of data. The result is significant changes after implementing three class actions, especially in the student-centred orientation of learning science. With worksheets supported by animated video media, everything strengthens the contextualization of the Water Cycle material to be closer to students' daily phenomena. Various activities and learning experiences are realized when implementing the CTL model's seven learning steps (syntax). At the end of the cycle, student engagement could increase significantly. Thus, implementing CTL assisted by video animation could improve the learning process and engagement.
Strategi Unggul Penguatan Kapasitas Logistik Perdagangan: Rencana Lima Tahun 2024-2029 Vikaliana, Resista; Iskandar, Yelita Anggiane; Puspitasari, Eka; Febryan, Rio
Transparansi : Jurnal Ilmiah Ilmu Administrasi Vol. 7 No. 2: Desember 2024
Publisher : Institut Ilmu Sosial dan Manajemen STIAMI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31334/transparansi.v7i2.4328

Abstract

Increasingly fierce global economic competition requires countries to develop effective trade strategies. In 2023, Indonesia improved its competitiveness ranking from 44th to 34th place according to the IMD Global Competitiveness Index. However, this position is still below Malaysia (27th) and Thailand (30th). This research uses a mixed quantitative and qualitative approach to analyze competitiveness indicators such as technology, health, labor market, and financial system. SWOT analysis was used to identify strengths, weaknesses, opportunities and threats in Indonesia's trade strategy. Critical success factors (CSF) analysis was used to structure information needs in the management hierarchy. By considering the general and specific conditions of Indonesia's trade logistics, a trade policy and strategy can be formulated. The trade strategy obtained from SWOT and CSF analysis is: 1. Strengthening downstream trade policies, 2. Strengthening multi-sectorregulatory stakeholder cooperation, in integrated logistics and trade marketing policies, 3. Accelerating digitalization and developing a competitive logistics service ecosystem, 4. Reorganizing trade logistics systems and facilities and infrastructure.Therefore, accurate and strategic trade planning is essential to improve economic efficiency and effectiveness, as well as competitiveness in the global market.
Antibacterial actifity of kecombrang (Etlingera elatior) leaf ethanol extract to against Klebsiella pneumoniae Puspitasari, Eka; Diah Pratiwi, Chalies; Rival Novian, Dede; Dayu Rahma Turista, Dora
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Vol. 7 No. 01 (2022): Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Publisher : Biology Education Department, Universitas Insan Budi Utomo, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33503/ebio.v7i01.1867

Abstract

Infectious diseases caused by bacteria in generals can be cured using antibacterial. The use of antibacterials tends to cause local hypersensitivity either on the skin or mucous membranes so that their use is starting to be reduced. Therefore, the development of natural antibacterials made from plants to reduce resistance is very necessary. kecombrang (Etlingera elatior) is one of the plants that has been shown to have activity in inhibiting bacterial growth. This research is an experimental study that aims to determine the effectiveness of the ethanol extract of the leaves of kecombrang in inhibiting the growth of Klebsiella pneumoniae bacteria in vitro. The method used is the well method with a completely randomized design. The sample came from the Klebsiella pneumoniae bacteria culture. Kecombrang leaf ethanol extract was made in concentrations of 20%, 40%, 60%, 80% and 100%. The extract was tested onKlebsiella pneumoniae bacteria and observed for the formation of inhibition zones. Inhibition the results of the zone measuremens were then analyzed descriptively. The results showed kecombrang leaf ethanol extract 20%, 40%, 60%, 80%, and 100% produce inhibition zone diameter 1.9 mm; 2.6 mm; 7.7 mm; 12.1 mm; and 14.7 mm, 0.4 mm for the negative control and positive control 22.0 mm. Concentration 100% has better antibacterial ability due to higher compound content. The test results show the class of secondary metaboliteskecombrang leaf ethanol extract contains flavonoids, tannin, and saponin. The conclusion of this study that the ethanol extract of kecombrang leaves