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REGULASI CATECHINS GREEN TEA GMB-4 DALAM MENGHAMBAT OKSIDASI NADPH DAN PENIGKATAN EKSPRESI NITRIC OXIDE (NO) PADA KULTUR HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS (HUVEC) YANG MENGALAMI STRES OKSIDATIF Peristiowati, Yuly; -, Indasah
Eduhealth Vol 3, No 1 (2013): Jurnal Eduhealth
Publisher : Eduhealth

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Abstract

ABSTRAKPenanganan kondisi disfungsi endotel sebelum terjadi kerusakan lebih lanjut sangatlah penting, karena berakibat terjadinya resistensi insulin dan hiperglikemia. Diharapkan dengan pemberian Catechins Green tea GMB-4 dapat memperbaiki kerusakan endotel melalui penghambatan oksidasi NADPH dan peningkatan produksi NO sehingga dapat mencegah terjadinya komplikasi lanjut pada pasien dengan diabetes Millitus. Desain penelitian ini true eksperimen dengan pendekatan post test only control group design. Subyek penelitian ini adalah kultur sel endotel yang berasal dari Human Umbilical Vein Endothelial Cell (HUVEC) yang dipapar glukosa 30 mM dan diberi perlakuan dengan Catechins Green tea GMB-4 dengan 3 dosis yaitu 0,03 mg/ml;0,3 mg/ml dan 3 ng/ml. Setelah pemberian selama 12 jam selanjutnya dilakukan analisa kadar NADPH dan Nitric Oxide (NO) dengan metode Colorimeter menggunakan NADPH Assay kit ab65349 dan Nitric Oxide Assay kit KGE001.Hasil penelitian menunjukan ada penurunan kadar NADPH pada kultur HUVEC setelah perlakuan Catechins Green tea GMB-4 pada dosis 0,3 mg/ml, dan terjadi peningkatan kadar Nitric oxide pada perlakuan dosis 0,03 mg/ml dan dosis 0,3 mg/ml. Hasil uji statistic one way Anova didapatkan nilai signifikan 0,000 untuk kadar NO dan NADPH. Sehingga dapat di simpulkan pemberian Catechins Green tea GMB-4 pada dosis 0,3 mg/ml dapat menurunkan oksidasi NADPH dan meningkatkan produksi NO secara signifikan. Pemberian Catechins Green tea GMB-4 dapat mengaktivasi eNOS dan berperan dalam penghambatan oksidasi NADPH melalui pospotidylinositol-3 kinase, cAMP dependent protein kinase dan jalur Akt. Catechins juga mengkatalisis produksi NO dari L-arginin dan menghambat ROS dengan jalan mengikat ion metal pada reaksi redok, menghambat transkripsi faktor, menghambat enzim prooksidan dan meningkatkan enzim antioksidan.Kata kunci: antioksidan, Catechins Green tea GMB-4, NO, NADPH ABSTRACTHandling conditions of endothelial dysfunction before further damage is very important, because it results in insulin resistance and hyperglycemia. Expected by administration of Green Tea Catechins GMB-4 can improve endothelial damage through inhibition of NADPH oxidation and NO production increased so as to prevent the occurrence of further complications in patients with diabetes millitus. True experimental design of this study to approach post-test only control group design. The subjects of this study were cultured endothelial cells derived from Human Umbilical Vein Endothelial Cell (HUVEC) were exposed to 30 mM glucose and treated with Green Tea Catechins GMB-4 with 3 doses are 0.03 mg / ml, 0.3 mg / ml and 3 ng / ml. Analized NADPH levels and Nitric Oxide (NO) with Colorimeter method using NADPH Assay kit ab65349 and Nitric Oxide Assay kit KGE001. The results showed decrease in NADPH levels in cultured HUVEC after treatment of Green Tea Catechins GMB-4 at a dose of 0.3 mg / ml, and an increase in levels of nitric oxide in the treatment dose of 0.03 mg / ml and 0.3 mg / ml . Results of one way ANOVA statistical test obtained significant value of 0.000 for the levels of NO and NADPH.. So it can be concluded giving Green tea Catechins GMB-4 at a dose of 0.3 mg / ml can reduce the oxidation of NADPH and increased NO production significantly. Provision of Green tea Catechins GMB-4 can activate eNOS and play a role in the inhibition of NADPH oxidation through pospotidylinositol-3 kinase, cAMP dependent protein kinase and Akt pathways. Catechins also catalyze the production of NO from L-arginine and inhibited ROS by binding the metal ions on the reaction redok, inhibits transcription factor, inhibits prooxidant enzymes and increases antioxidant enzymes.Keywords: antioksidant, Catechins Green tea GMB-4, NO, NADPH
The Effects of Concentration and Fermentation Time on Quality of Local Microorganism Solutions (LMS) of Stale Rice, Cassava “Tape”, Banana Bumps and Cow’s Rumen or Rotten Fruits indasah, indasah; Peristiowati, Yuli; Nurwijayanti, Nurwijayanti
Health Notions Vol 1 No 3 (2017): July-September 2017
Publisher : Humanistic Network for Science and Technology (Address: Cemara street 25, Ds/Kec Sukorejo, Ponorogo, East Java, Indonesia 63453)

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Abstract

The objective of this research was to analyze the quality of bioactivator from stale rice, cassava tape, banana bumps, cow”s rumen / rotten fruit, based on concentration and fermentation time, with factorial design. The first factor was the concentration of stale rice, tape, banana bumps, cow”s rumen or rotten fruit, which was 150 grams and 250. The second factor was fermentation time consisting of: F1 (Fermentation for 10 days), F2 (Fermentation for 20 days). Parameters observed were: total population of bacteria, pH and temperature. Data were analyzed using Anova. The results showed that the highest result of total bacterial population was found on 150 gram banana bumps with fermentation of 20 days ie 41 x 106 and the lowest on 150 gram cassava tape with 10 day fermentation that was 1x102. The highest pH was found on rice with 250 grams of fermented rice with 20 days of 3.00 and the lowest on 250 grams of banana bumps on day 0 which was 5.96. The highest temperature on stale rice was 150 gram with 10 days fermentation, 150 banana bumps and 250 gram with 10 day fermentation of 29.5 0C and the lowest temperature on 150 gram cassava tape, rumen / rotten fruit of 150 gram on day 0 was 27 0C. Anova analysis showed no difference in the number of germs from the eight groups.
The Early Stimulation, Detection and Intervention of Child Growth and Development in Balowerti Public Health Center Kumalasari, Eri Puji; Indasah, Indasah; Suhita, Byba Melda
Health Notions Vol 2 No 7 (2018): July 2018
Publisher : Humanistic Network for Science and Technology (Address: Cemara street 25, Ds/Kec Sukorejo, Ponorogo, East Java, Indonesia 63453)

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abstract
The Effectiveness of Family Centered Maternity Care (FCMC) Education to Increase The Independence of Pospartum Mother Mayasari, Senditya Indah; Suhita, Byba Melda; Indasah, Indasah
Health Notions Vol 2 No 5 (2018): May 2018
Publisher : Humanistic Network for Science and Technology (Address: Cemara street 25, Ds/Kec Sukorejo, Ponorogo, East Java, Indonesia 63453)

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Abstract

Family Cenetered Maternity Care (FCMC) Education is family-centered care by providing care for women and their families that integrate pregnancy, delivery, childbirth, and infant care into the continuum of the family life cycle as usual through healthy living. The care given to the individual prioritizes the support, participation, and choice of the family. Treatments that need to be done in the puerperium are breast care for breastfeeding and postpartum perineal care to avoid infection. The study was conducted with the aim to know the Effectiveness of Family Centered Maternity Care (FCMC) Education in Increasing Independence of Postpartum Mother in Malang Regency. The research type is true experimental with Pretest-Posttest Design approach. Sampling is done by systematic random sample. Data collection was obtained with questionnaires to postpartum physiologic mothers as many as 12 postpartum mothers in the intervention and control group. Result showed after being educated almost all (92%) of respondents have been self-sufficient in breast care, independence in perineal care at 83% independent level and 8% light dependence. FCMC education can increase independence in care needs during the puerperium. Breast care is done to prepare the lactation process, especially during the puerperium for the babys nutritional needs. While perineum treatment is done to maintain hygiene and prevent infection in the vulva, perineum in the uterus and perineum wound healing. Keywords: Postpartum, FCMC, Self-reliance, Breast care, Perineal care
The Act of PMTCT Program to decrease The Numbers of HIV in Situbondo Reffita, Lea Ingne; Suhita, Byba Melda; Indasah, Indasah
Health Notions Vol 2 No 6 (2018): June 2018
Publisher : Humanistic Network for Science and Technology (Address: Cemara street 25, Ds/Kec Sukorejo, Ponorogo, East Java, Indonesia 63453)

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Abstract
PELATIHAN DAN PEMBINAAN HEALTH AND HYGIENE EDUCATION (HHE) BAGI PENGGUNA SANIMAS DI KOTA KEDIRI Indasah, Indasah; Fazrin, Intan; Rachmathiany, Ridha
Jurnal Abdi Masyarakat Vol 2, No 2 (2019): Mei 2019
Publisher : Universitas Kadiri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30737/jaim.v2i2.367

Abstract

Kegiatan Pengabdian untuk pelatihan, pembinaan pada kelompok swadaya masyarakat “KSM” SANIMAS (sanitasi Berbasis Masyarakat) di Kota Kediri . Kegiatan ini bertujuan untuk: meningkatkan kesadaran tentang permasalahan kesehatan dan hygiene dengan menyediakan informasi yang tepat dan mempromosikan praktek kesehatan dan hygiene yang baik. Kegiatan ini dilakukan dengan metode focus grup discussion (FGD) sehingga partisipan dapat mengemukakan permasalahan dan mendapatkan solusi yang tepat. Hasil kegiatan ini partisipan mendapat informasi tentang sanimas dan health hygiene education (HHE) yang dapat memberdayakan masyarakat untuk mencegah penyakit terkait sanitasi, memperbaiki perilaku kesehatan dan hygiene, mencegah penyakit diare dengan memutus rute kontaminasi,  memberikan pengetahuan yang tepat mengenai perawatan fasilitas sanitasi dan inovasi dari pengolahan limbah seperti halnya dalam pembuatan biogas, program hidroponik, budidaya ikan,  tempat mandi cuci kakus yang indah untuk sarana edukasi serta pemahaman  dan keterampilan organisasi dan perawatan  SANIMAS.  Dukungan kerjasama pemerintah kota, lintas sektor dan masyarakat sehingga mewujudkan kota Kediri bebas dari buang air besar sembarangan, lingkungan yang sehat, bersih, indah, dan bermanfaat.
The Effectiveness of Family Centered Maternity Care (FCMC) Education to Increase The Independence of Pospartum Mother Senditya Indah Mayasari; Byba Melda Suhita; Indasah Indasah
Health Notions Vol 2, No 5 (2018): May
Publisher : Humanistic Network for Science and Technology (HNST)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (247.322 KB) | DOI: 10.33846/hn.v2i5.205

Abstract

Family Cenetered Maternity Care (FCMC) Education is family-centered care by providing care for women and their families that integrate pregnancy, delivery, childbirth, and infant care into the continuum of the family life cycle as usual through healthy living. The care given to the individual prioritizes the support, participation, and choice of the family. Treatments that need to be done in the puerperium are breast care for breastfeeding and postpartum perineal care to avoid infection. The study was conducted with the aim to know the Effectiveness of Family Centered Maternity Care (FCMC) Education in Increasing Independence of Postpartum Mother in Malang Regency. The research type is true experimental with Pretest-Posttest Design approach. Sampling is done by systematic random sample. Data collection was obtained with questionnaires to postpartum physiologic mothers as many as 12 postpartum mothers in the intervention and control group. Result showed after being educated almost all (92%) of respondents have been self-sufficient in breast care, independence in perineal care at 83% independent level and 8% light dependence. FCMC education can increase independence in care needs during the puerperium. Breast care is done to prepare the lactation process, especially during the puerperium for the baby's nutritional needs. While perineum treatment is done to maintain hygiene and prevent infection in the vulva, perineum in the uterus and perineum wound healing. Keywords: Postpartum, FCMC, Self-reliance, Breast care, Perineal care
Early Stimulation, Detection and Intervention for Growth and Development of Children (ESDIGDC) for Preschooler in Balowerti Health Center, Kediri Eri Puji Kumalasari; Indasah Indasah; Byba Melda Suhita
Health Notions Vol 2, No 7 (2018): July
Publisher : Humanistic Network for Science and Technology (HNST)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/hn20706

Abstract

Early Stimulation, Detection and Intervention for Growth and Development of Children (ESDIGDC) is an effort to monitor and collect and find early existence of irregularities and prevent the incidence of permanent disturbance through examination of growth, development and mental emotional condition of children. The objective of the study was to explore the implementation of ESDIGDC program in preschool children In Balowerti Health Center, Kediri. The study design used was qualitative research with phenomenology approach. Sampling technique in this research was purposive sampling with the sample size of 6 informants. Data were collected using indepth interview technique with semi-structured question. The result of the research shows that the implementation of ESDIGDC program influenced the development of ESDIGDC program in every year and will cause positive impact for both health worker and preschooler. The process of ESDIGDC program implementation was influenced by several factors. Based on the research, further research on the implementation of ESDIGDC program is needed. Keywords: ESDIGDC; preschooler; growth; development
The Act of PMTCT Program to Decrease the Numbers of HIV in Situbondo Regency Lea Ingne Reffita; Byba Melda Suhita; Indasah Indasah
Health Notions Vol 2, No 6 (2018): June
Publisher : Humanistic Network for Science and Technology (HNST)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/hn20608

Abstract

PMTCT is an effort to prevent the occurrence of HIV infection in women and prevent HIV transmission from pregnant mother to baby. The number of women infected with Human Immunodeficiency Virus (HIV) from year to year has increased, along with the increasing number of men who have unprotected sexual intercourse, which will transmit HIV to their sexual partners. The purpose of this research is to explore the implementation of PMTCT program in Situbondo regency. The research design used was qualitative research with phenomenology approach. Data collection used indepth interview technique with semi-structured question. Sampling technique in this research used purposive sampling with the sample size of 8 informants from 17 informants. From the result of the research showed that the implementation process of PMTCT program influences the development of PMTCT program in every year and will cause positive impact for both health worker and pregnant woman. In the process of PMTCT program implementation was influenced by several factors, such as, there were factors that support from the implementation process of PMTCT program as well as heading off the factors. From these inhibiting factors, several strategies were used to support the PMTCT program. Based on the research, it is necessary to conduct further research on the implementation process of PMTCT program Keywords: PMTCT (prevention of mother to child HIV transmission); Human Immunodeficiency Virus (HIV)
The Effects of Concentration and Fermentation Time on Quality of Local Microorganism Solutions (LMS) of Stale Rice, Cassava “Tape”, Banana Bumps and Cow’s Rumen or Rotten Fruits Indasah Indasah; Yuly Peristiowati; Nurwijayanti Nurwijayanti
Health Notions Vol 1, No 3 (2017): July-September
Publisher : Humanistic Network for Science and Technology (HNST)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/hn.v1i3.36

Abstract

The objective of this research was to analyze the quality of bioactivator from stale rice, cassava tape, banana bumps, cow”s rumen / rotten fruit, based on concentration and fermentation time, with factorial design. The first factor was the concentration of stale rice, tape, banana bumps, cow”s rumen or rotten fruit, which was 150 grams and 250. The second factor was fermentation time consisting of: F1 (Fermentation for 10 days), F2 (Fermentation for 20 days). Parameters observed were: total population of bacteria, pH and temperature. Data were analyzed using Anova. The results showed that the highest result of total bacterial population was found on 150 gram banana bumps with fermentation of 20 days ie 41 x 106 and the lowest on 150 gram cassava tape with 10 day fermentation that was 1x102. The highest pH was found on rice with 250 grams of fermented rice with 20 days of 3.00 and the lowest on 250 grams of banana bumps on day 0 which was 5.96. The highest temperature on stale rice was 150 gram with 10 days fermentation, 150 banana bumps and 250 gram with 10 day fermentation of 29.5 0C and the lowest temperature on 150 gram cassava tape, rumen / rotten fruit of 150 gram on day 0 was 27 0C. Anova analysis showed no difference in the number of germs from the eight groups.Keywords: Concentration, Fermentation, Local Microorganism Solutions,  Stale Rice, Cassava “Tape”, Banana Bumps, Cow’s Rumen, Rotten Fruits
Co-Authors A.A. Ketut Agung Cahyawan W Adelina Pinto Adolfina Sating Afriyani, Nur Agung Setyanto Agus Setiyawan Agusta Dian Elliana Agusta Dian Ellina Agustin Widyowati Agustinus Bali Matkusa Al Annas, Siti Nur Nasruah Amarin Yudhana Amarin Yudhana, Amarin Andin Ajeng Rahmawati Anggraeni, Novita Anna Anis Ansyori Anita Restu Korbaffo Anna Nurhidayati Anom Putrika, Ida Ayu Arfah, Andi Muh Arief Rheegandono Asasih Villasari Asnuddin, Asnuddin Asyaul Wasiah Atik Setiawan Wahyuningsih Atmojo, Joko Tri Bambang Priyono Billy Anoraga, Aditya Budi Andayani Byba Melda Byba Melda Byba Melda Suhita Byba Melda Suhita Byba Suhita Cahyono, Ibnu Chalies Diah Pratiwi Danang Sasongko Darwis, Raodatul Jannah Dedi Saifulah Desty Damayanti Dewi K, Prima Dewi Zuniawati Dian Hadi Purnomo Eliyawati Eliyawati Ellina, Agusta Dian Elly Setyowati Ellysa Okky Gusma Ellysa Okky Gusma Enggar Wahyu Anggraeni Erwan Darmawan Erwin, Erwin Evita Widyawati Evrin Angga Fadilatul, Dewi Faisal Isra Maulana Farasari, Poppy Fardiansyah, Arief Fathor Rahman Fatmawati, Fatmawati Fazrin, Intan Feryansah, Ade Fika Istikah Fitri Handayani Fitriani Fitriani Fresvian Jenrivo Gempi Tri Sumini Gretta Hapsari Amalya Handayani, Rina Tri Hargiani, Fransisca Xaveria Hari Prasetyo umar Hari, Prasetyo Umar Hariyani Hariyani Hariyanto Hariyanto Hasbyalloh, Mochamad Salman Heny Pramuta Sari Heri Saputro Herry Krismono Herwati Herwati Hidayat, Useng Hilda Anggraeni Laluyan Ida Ayu Anom Putrika Ida Vira Wati Ida Watna Iing Yuli Indrawati Indra Cahyadinata, Indra Indrawati, Iing Yuli Intan Fazrin Intan Herlinawati Irawan, Atok Iriawan Putra, Pujo Broto Joaquim Pinto Joko Prasetyo Joko Prasetyo Joko Sasmito Katmini Katmini, Katmini Kentarti, Rima Khairul Anwar, Raden Bagus Khomsiyatin, Binti Ratna khotib, akhmad Koesnadi Koesnadi Koesnadi, Koesnadi Koesnadi, Koesnadi Koesnadi, Koesnadi Kumalasari, Eri Puji Kurnianti, Nur Rochmah Kusumaningsih, Emy Kusumawardani, Nila Lalu Dedy Supriatna Lea Ingne Reffita Lesmana, Iwan Setyo Linda Sukoco Listyorini Wulandari Maharjan, Kailie Maria Haryanti Butar-Butar Mariyati, Titik Mark, Elladdadi Maruapey, Erna Rosiyawati Mas Winarto Mayasari, Senditya Indah Meihindra Cahyo Suci Wardoyo Meiningsih, Meiningsih Melda Byba Suhita Melda Suhita, Byba Mieke Waluya Waty Misrani, Misrani Mitakda, Esterlina Mochamad Salman Hasbyalloh Mohamad As'ad Efendy Muhamad Rizal Amin Muhammad Ali Sodik Muslim, Asep Aziz Nancy Natalia Dharmawan Nia Sari Nia Sari Noor Laila, Siti Farida Novita Anna Novita Anna Novita Anna Anggraeni NOVITA EKA SARI Nugrahani, Fransiska Herningtiyas Ayu Nurdin, Sulkifli Nurdina Nurdina Nurhidayati, Anna Nurwijayanti Nurwijayanti Kusumaningrum Nurwijayanti Nurwijayanti3 Octaviana Marta G.M. Gusmão Ofrin Sammytampani Oktafianus Radja Tuka Pamungkas, Restu Peristiowati, Yuli Poppy Farasari Poppy Hidayat Pramono Pramono Prananingtias, Rosita Prananto, Bintang Arsy Wahyu Pratami, Mardiah Pratiwi, Chalies Diah Pratiwi, Wilda Rezki Prima Dewi Kusumawati Priyanti, Ida Pujo Broto Iriawan Putra Purnomo, Dian Hadi Putri Eka Sejati Putri Eka Sejati Rachmathiany, Ridha Rahmania Ambarika Rahmi Saptarianti Ratna Wardani Ratna Wardani Raudatul Hikmah Raudatul Hikmah Reffita, Lea Ingne Respati, Laras Laxita Winda Reza Diko Utama Rheegandono, Arief Ridha Rachmathiany Rindang Diannita Rini Setyowati Rini Sulistyoasih Ririt Ika Lestari Risa Nurhayati Risa Nurhayati Risyan Karnain Risyan Karnain Rita Sugihati Rizal Maulana, Rizal Rodli, Muhammad Rohani, Muhammad Rosarini, Anna Rumangun, Kristo Marco Ryan Hidayatullah Sa'diyah, siti aminatus sa'diyah Samad, Andi Muh. Arfah Saputra Sandu Siyoto Sandu Siyoto, Sandu Santa Betty Santino Dacosta Barros Sari, Nia Sentot Imam Suprapto Sentot Imam Suprapto Sentot Imam Suprapto Sentot Imam Suprapto Setiyaningrum, Erna Shella Dhika Rahmawati Silvia Dwi Agustin Siswanti, Siswanti Siti Farida Siti Farida Noor Laila Siti Farida Noor Layla Siti Solikha Sudewa, I Gusti Ngurah Wisnu Sugiyono Sugiyono Suhita, Byba Sukartana, Sukartana Sumiati Sumiati Sumiati Suprapto, Sentot Imam Suprapto, Sentot Imam Suprapto, Sentot Imam Suripto Suripto Suwono, Yohanes Adi Agus Syamsul Arifin Takariningsih Teguh Achmalona Tety Yuliantine Tri Retnoningsih Vian Eka Putra Wardani , Ratna Warna, Mawar Puspa Waty, Mieke Waluya Widiyanto, Aris Wijaya, Muhammad Irzal winarko, winarko Yammar, Yammar Yenny Puspitasari Yenny Puspitasari Yenny Puspitasari Yohana Teodosia Setu Yuli Peristiowati, Yuli Yuliadi Setiawan Yuliantine, Tety Yuliawati, M. Ali Yuliawati, Riza Yuly Peristiowati Yuly Peristiowati Yuly Peristiowati Yunita Yunita Zahro, Siti Lailatus Zahrotur Rubbiyani