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All Journal Jurnal Kesehatan Masyarakat Journal of Biomedicine and Translational Research
Hidajat, M Choirul
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Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology Public Health

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DETEKSI YERSINIA PESTIS DAN PATOGEN ZOONOTIK LAINNYA PADA TIKUS DI DAERAH FOKUS PES DESA KAYUKEBEK KABUPATEN PASURUAN Mulyono, Arief; Hidajat, Muhammad Choirul; Ristiyanto, Ristiyanto; Setyaningtyas, Dian Eka; Wigati, RA; Putro, Dimas Bagus Wicaksono; Joharina, Arum Sih; Pratiwi, Ayu Pradipta; Muhidin, Muhidin; Yuliadi, Bernardus
Jurnal Kesehatan Masyarakat Vol 12, No 2 (2024): MARET
Publisher : Fakultas Kesehatan Masyarakat Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkm.v12i2.40602

Abstract

Rats are known as carriers and transmissions of zoonotic diseases. About 60 types of zoonotic diseases that rats can transmit to humans. Some zoonotic diseases are Plague, Leptospirosis, Orthohantavirus infection, and Hepatitis E virus. This research aims to detect the bacteria Yersinia pestis, Leptospira, Hepatitis E virus, and Orthohantavirus in rats in Kayu Kebek Village, Pasuruan Regency. The research design used in this study is descriptive with a cross-sectional approach. The rats were captured for three days, two nights in the house, the neighborhood around the house, and the forest. The captured mice were identified as specimens of blood, spleen, kidneys, and liver. Detection of Yersinia pestis, Leptospira, and Hepatitis E is performed using PCR, while the detection of Orthohantavirus is performed using the ELISA method. A total of 45 mice were trapped, consisting of 2 genera and three species. The zoonotic pathogens detected were Leptospira and hepatitis E. Leptospira viruses were detected in Rattus tanezumi, Rattus tiomanicus, and Niviventer fluvescent. Hepatitis E virus is detected only in R. tanezumi. Transmission of leptospirosis and hepatitis E has the potential to occur in Kayu Kebek Village. Rat control is needed to prevent the transmission of leptospirosis and hepatitis E virus infection, and other zoonotic diseases transmitted by rats.
Detection of Pathogenic Leptospira in Sputum of Leptospirosis Patient with Pulmonary Hemorrhage. Handayani, Farida Dwi; Novipuspitasari, Lisa; Ahmed, Ahmed; Safari, Dodi; Hidajat, Muhammad Choirul; Soebandrio, Amin; Gasem, Muhammad Hussein
Journal of Biomedicine and Translational Research Vol 11, No 2 (2025): August 2025
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v11i2.28259

Abstract

Background: The pathogenic Leptospira species is the causative agent of leptospirosis, an endemic zoonotic disease in Indonesia. Misdiagnosis of the disease frequently occurs, as confirmatory diagnosis confined to highly specialized laboratories. As well, the pulmonary involvement of leptospirosis with hemoptysis is scarcely reported.Case Presentation: A 49 years-old male patient was admitted to the district hospital with acute febrile illness and a history of traveling to a malaria-endemic area in Borneo, Indonesia. Based on a chest X-ray result, the patient was clinically suspected to have pulmonary tuberculosis. However, the clinical manifestations of leptospirosis i.e. conjunctival suffusion, calf pain, and oliguria were present, and later hemoptysis was also reported. A clinical diagnosis of leptospirosis with pulmonary involvement was proposed. Immunochromatographic test (ICT)-rapid test for vivax/falciparum malaria and Ziehl-Neelsen (ZN) staining of sputum for tuberculosis results were both negative. Microscopic Agglutination Test (MAT), the IgM anti-Leptospira rapid test (lateral flow assay), and PCR amplification of both conventional and real-time (qPCR) were performed using various samples (serum, urine, and sputum). The MAT of acute single serum sample and rapid test were negative. Intriguingly, the PCR showed positive results in sputum and urine samples but not in the serum sample, highlighting the usefulness of leptospiral molecular detection to confirm further diagnosis.Conclusion: Molecular detection of pathogenic Leptospira in sputum samples can be considered for confirmatory diagnosis of leptospirosis patients with pulmonary hemorrhage. Likewise, the urine sample can be used as an option in the examination of severe leptospirosis.
Co-Authors Ahmed, Ahmed Amin Soebandrio Arief Mulyono Arum Sih Joharina Dian Eka Setyaningtyas Dimas Bagus Wicaksono Putro, Dimas Bagus Wicaksono Farida Dwi Handayani Gasem, Muhammad Hussein Ida Ayu Putu Sri Widnyani Novipuspitasari, Lisa Pratiwi, Ayu Pradipta RA Wigati Ristiyanto Ristiyanto Safari, Dodi Yuliadi, Bernardus