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All Journal Jurnal Kesehatan Masyarakat Jurnal EnviScience (Environment Science) Bioscientist : Jurnal Ilmiah Biologi Berita Biologi
Arief Mulyono
Balai Besar Penelitian dan Pengembangan Vektor dan Reservoir Penyakit Salatiga
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Education Engineering Immunology & microbiology Public Health Social Sciences

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DETEKSI YERSINIA PESTIS DAN PATOGEN ZOONOTIK LAINNYA PADA TIKUS DI DAERAH FOKUS PES DESA KAYUKEBEK KABUPATEN PASURUAN Mulyono, Arief; Hidajat, Muhammad Choirul; Ristiyanto, Ristiyanto; Setyaningtyas, Dian Eka; Wigati, RA; Putro, Dimas Bagus Wicaksono; Joharina, Arum Sih; Pratiwi, Ayu Pradipta; Muhidin, Muhidin; Yuliadi, Bernardus
Jurnal Kesehatan Masyarakat Vol 12, No 2 (2024): MARET
Publisher : Fakultas Kesehatan Masyarakat Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkm.v12i2.40602

Abstract

Rats are known as carriers and transmissions of zoonotic diseases. About 60 types of zoonotic diseases that rats can transmit to humans. Some zoonotic diseases are Plague, Leptospirosis, Orthohantavirus infection, and Hepatitis E virus. This research aims to detect the bacteria Yersinia pestis, Leptospira, Hepatitis E virus, and Orthohantavirus in rats in Kayu Kebek Village, Pasuruan Regency. The research design used in this study is descriptive with a cross-sectional approach. The rats were captured for three days, two nights in the house, the neighborhood around the house, and the forest. The captured mice were identified as specimens of blood, spleen, kidneys, and liver. Detection of Yersinia pestis, Leptospira, and Hepatitis E is performed using PCR, while the detection of Orthohantavirus is performed using the ELISA method. A total of 45 mice were trapped, consisting of 2 genera and three species. The zoonotic pathogens detected were Leptospira and hepatitis E. Leptospira viruses were detected in Rattus tanezumi, Rattus tiomanicus, and Niviventer fluvescent. Hepatitis E virus is detected only in R. tanezumi. Transmission of leptospirosis and hepatitis E has the potential to occur in Kayu Kebek Village. Rat control is needed to prevent the transmission of leptospirosis and hepatitis E virus infection, and other zoonotic diseases transmitted by rats.
Identifikasi Spesies, Kepadatan, dan Pinjal pada Tikus di Area Parkir dan UC-Walk Universitas Ciputra Surabaya Adrianto, Hebert; Liemdjaja, Maynard Adam; Eric Dino Tandoyo; Silitonga, Hanna Tabita Hasianna; Yuwono, Natalia; Ristiyanto; Mulyono, Arief; Wigati
Jurnal EnviScience (Environment Science) Vol. 9 No. 1 (2025): The Environment In Global Health Governance:An Analysis of Environment-Related
Publisher : Universitas Islam Lamongan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30736/jev.v9i1.813

Abstract

Tikus dapat menjadi inang bagi patogen penyebab penyakit. Leptospirosis dapat ditularkan melalui air liur dan urin tikus, sementara gigitan pinjal pada tubuh tikus dapat menyebabkan wabah pes. Tujuan dari penelitian ini adalah untuk mengidentifikasi spesies tikus, indeks populasi tikus, dan pinjal pada tikus di area parkir dan UC-walk. Penelitian dilakukan di lokasi-lokasi tersebut karena memiliki potensi untuk menjadi habitat bagi tikus. Penelitian ini merupakan studi observasional deskriptif dengan desain potong lintang, yang dilaksanakan dari September 2024 hingga Oktober 2024. Perangkap dipasang antara pukul 18:00-19:00 WIB, dengan total 10 perangkap hidup yang ditempatkan di titik-titik yang sudah ditentukan di setiap lokasi. Analisis kepadatan tikus menggunakan rumus indeks populasi tikus, yakni memenuhi syarat jika nilai indeks populasi tikus <1. Bulu tikus disisir untuk mencari pinjal. Pinjal diamati di bawah mikroskop, dan identifikasi spesies dilakukan. Identifikasi tikus dilakukan di lembaga BRIN. Hasil penelitian didapatkan enam tikus dengan spesies Rattus tanezumi dan Rattus norvegicus dengan trap success sebesar 0,6 dan 0. Hasil dari trap success yang didapat jika dibandingkan dengan standar baku mutu maka masih memenuhi standar. Hasil dari penyisiran pinjal terdapat satu pinjal dengan jenis Xenopsylla cheopis. Penelitian ini menunjukan rendahnya angka keberadaan tikus pada Universitas Ciputra sehingga kebersihan dan sanitasi pada Universitas Ciputra terjaga.  
Insidensi Ortohantavirus pada Inang Reservoir di Kota Semarang, Jawa Tengah Septiyani, Rani Dwi; Dhanti, Kurnia Ritma; Kurniawan, Kurniawan; Mulyono, Arief; Handayani, Farida Dwi
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.11591

Abstract

Orthohantavirus is an RNA virus that causes Hemorrhagic Fever with Renal Syndrome (HFRS) and Hantavirus Pulmonary Syndrome (HPS). Humans can become infected with orthohantaviruses by inhaling virus particles from the urine, saliva, and feces of infective reservoir hosts. Small mammals from the orders Rodensia, Eulipotyphla, and Chiroptera are the main reservoirs of orthohantaviruses. This study aims to detect and calculate the incidence of orthohantavirus in reservoir hosts in Semarang City. The research was conducted descriptively analytically with a cross-sectional study design. The research population was all mice and shrews in the research location (Gayamsari and Tlogosari subdistricts), and the samples were caught mice and shrews (84). The research was carried out in May-September 2023. Orthohantavirus detection was carried out using the Nested PCR test (pre denaturation, denaturation, annealing and extension). A total of 84 individuals were captured, consisting of Rattus novergicus, Rattus tanezumi and Suncus murinus. Orthohantavirus in this study was successfully detected in Rattus norvegicus. The results of the study showed that the incidence of orthohantavirus in Gayamsari Village was (11.1%), Tlogosari Village (2.78%), and the overall total was (2.38%). It is necessary to control mice to prevent transmission of orthohantavirus.
TUNGAU VEKTOR DEMAM SEMAK PADA TIKUS DI CALON IBU KOTA NEGARA, KALIMANTAN TIMUR Marsongko, Wigati; Ristiyanto; Widayati, Anis N.; Mulyono, Arief; Garjito, Triwibowo A.; Handayani, Farida D.; Mujiyanto; Hidajat, Muhammad C.; Anggraeni, Yusnita M.; Wahono, Tri; Kesuma, Agung P.
Berita Biologi Vol 23 No 2 (2024): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/beritabiologi.2024.437

Abstract

Penelitian terkait fauna tungau trombikulid, yang merupakan vektor penyakit demam semak, telah dilakukan di calon Ibu Kota Negara, yaitu Kalimantan Timur. Tujuan penelitian ini adalah untuk mengetahui jenis tungau trombikulid dan jenis tikus sebagai inangnya di Kalimantan Timur. Penelitian bersifat deskriptif dan berlangsung di tahun 2019. Penelitian dilakukan dengan survei tikus di dalam dan luar rumah, juga tungau trombikulid pada tubuh tikus yang tertangkap. Hasil penelitian menunjukkan bahwa jenis tungau trombikulid pada tikus di calon Ibu Kota Negara terdiri atas 10 spesies yang termasuk dalam 6 genera. Berdasarkan hasil penelitian ini, disimpulkan bahwa daerah Kalimantan Timur berpotensi terjadi penularan demam semak oleh tungau trombikulid yang disebabkan oleh habitat spesifik tungau tersebut.
Co-Authors Arum Sih Joharina Dhanti, Kurnia Ritma Dian Eka Setyaningtyas Dimas Bagus Wicaksono Putro, Dimas Bagus Wicaksono Eric Dino Tandoyo Farida Dwi Handayani Handayani, Farida D. Hanna Tabita Hasianna Silitonga Hebert Adrianto Hidajat, M Choirul Hidajat, Muhammad C. Ida Ayu Putu Sri Widnyani Kesuma, Agung P. Kurniawan Kurniawan Liemdjaja, Maynard Adam Marsongko, Wigati Mujiyanto Natalia Yuwono Pratiwi, Ayu Pradipta RA Wigati Ristiyanto Ristiyanto Ristiyanto Septiyani, Rani Dwi Tri Wahono Triwibowo A. Garjito Widayati, Anis N. Wigati Yuliadi, Bernardus Yusnita M. Anggraeni, Yusnita M.