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All Journal Jurnal Ilmu dan Teknologi Kelautan Tropis Wikrama Parahita : Jurnal Pengabdian Masyarakat Jurnal Penelitian Pendidikan IPA (JPPIPA) Jambura Edu Biosfer Journal Jurnal Abdi Insani GANDRUNG: Jurnal Pengabdian Kepada Masyarakat Sciscitatio : Journal for Biological Science Jurnal Sains dan Edukasi Sains Indigenous Biologi Indonesian Collaboration Journal of Community Services (ICJCS) SWARNA SWARNA: Jurnal Pengabdian Kepada Masyarakat Berita Biologi
Rupidara, Anggreini D.N
Program Studi Pendidikan Biologi, FKIP, Universitas Kristen Artha Wacana, Kupang
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Journal : Indigenous Biologi

 1 
UJI KUALITAS AIR PADA SUMBER MATA AIR WAIPADDA DESA ANAJIAKA KECAMATAN UMBU RATU NGGAY BARAT KABUPATEN SUMBA TENGAH Noprianto Umbu Tiba Marak; Anggreini D.N Rupidara; Novi I Bullu
Indigenous Biologi : Jurnal Pendidikan dan Sains Biologi Vol 2 No 2 (2019): Indegenous Biologi
Publisher : Prodi Pendidikan Biologi FKIP Universitas Kristen Artha Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33323/indigenous.v2i2.38

Abstract

Abstrak Air merupakan bahan yang sangat vital yang tidak terpisahkan dari kehidupan mahkluk hidup di muka bumi ini.Segi kualitas, beberapa persyaratan yang harus dipenuhi sebagai air bersih yaitu parameter fisik ,kimia dan biologi.Salah satu mata air yang penggunaannya sangat vital bagi masyarakat Sumba Tengah adalah mata air Waipadda. Tujuan dari penelitian iniuntuk mengujikualitas mata air Waipada di desa Anajiaka kecamatan Umbu Ratu Nggay Barat kabupaten Sumba Tengah dengan berdasarkan parameter fisik, kimia dan biologi. Penelitian ini menggunakan metode eksperimen dan dianalisis secara deskriptif kuantitatif dengan membandingkan data hasil pengukuran dari masing-masing parameter air dengan nilai baku mutu PP No. 82 Tahun 2001.Berdasarkan hasil penelitian pada Mata Air Waipadda diperoleh uji kualitas air padaSuhu (23ºC),TDS (215mg/L),TSS (0,0014 mg/L),pH (7,40 mg/L), DO (5,12mg/L), BOD (1,65mg/L),besi (0,02 mg/L),nitrit (0,008 mg/L),nitrat (1,2 mg/L),sianida (0,009 mg/L), Klorin (0,01 mg/L), mangan (0,043 mg/L), sulfat (1 mg/L), dan uji total coliform(<1,8 jlm/100 ml) memenuhi standar batas baku mutu yang ditetapkan oleh pemerintah yaitu dikategorikan sebagai kelas I. Namun kadar (Tembaga 0,28 mg/L (0,02 mg/L) dan Phospat 0,27 mg/L (0,2 mg/L) melebihi standar baku mutu yang ditetapkan. Hal ini dipengaruhi oleh beberapa faktor seperti adanya irigasi (pertanian), pembusukan daun, dan aktivitas alam seperti erosi, pelapukan bebatuan/pengikisan batuan dan intensitas cahaya yang diterima oleh badan air rendah (kenaikan suhu).
UJI DAYA HAMBAT PEWARNA ALAMI KULIT BATANG JAMBLANG (Syzygium cumini) TERHADAP BAKTERI Escherichia coli, Staphylococcus aureus DAN JAMUR Aspergilus niger Febriana Bani Utang; Anggreini D.N Rupidara; Alan Ch Sabuna
Indigenous Biologi : Jurnal Pendidikan dan Sains Biologi Vol 3 No 3 (2020): Indigenous Biologi
Publisher : Prodi Pendidikan Biologi FKIP Universitas Kristen Artha Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33323/indigenous.v3i3.91

Abstract

ABSTRACTJamblang (Syzygium cumini) belongs to the family of the guava tribe which has benefits as a food ingredient as well as herbal medicines. Jamblang bark produces tanners (tannins) to color the nets. The purpose of this study was to determine the inhibition of natural dyes of jamblang (Syzygium cumini) bark against Escherichia coli, Staphylococcus aureus, and Aspergillus niger fungi. The method used in this study wis an experimental method, which consists of 3 treatments of natual dyes of the bark cuminis where 40%, 60%, and 80% consentrations and 3 replicates. Data of the inhibitory activity where tested using paper disk method and was analyzed statistically using ANOVA test with SPSS 16.0. statistic aplication. The natural dyes from each concentration showed a inhibitoryactivity against E. coli, S. aureus, and A. niger. The effective concentration of inhibitory activity in E. Coli was 60%, natural dyes category zonas 4.0 mm or weak inhibitory activity The effective concentration of inhibitory activity in S. aureus was 40%, natural dyes category zonas was 7.0 mm or moderateinhibitory activity. The effective concentration of inhibitory activity in A. Niger was 80% natural dyes category zonas was 4.0 mm or weak inhibitory activity.Kaywords :Antibacterial test,Jamblang (Syzygiumcumini) bark, natural coloring Escherichia coli, Staphylococcus aureus, Aspergillus niger.
SELEKSI ENZIM PROTEASE JAMUR ENDOFIT DAUN MANGROVE Avicennia marina DI PANTAI NOELBAKI Ludji Lobo, Ocsryn Lylianti; Rupidara, Anggreini D.N; Ledo, Mellissa E.S
INDIGENOUS BIOLOGI : JURNAL PENDIDIKAN DAN SAINS BIOLOGI Vol 5 No 3 (2022): Indigenous Biologi
Publisher : Prodi Pendidikan Biologi FKIP Universitas Kristen Artha Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33323/indigenous.v5i3.267

Abstract

ABSTRAK Jamur endofit dapat diartikan sebagai mikroba yang hidup berkoloni dalam jaringan internal makhluk hidup seperti pada daun tanpa menyebabkan efek yang merugikan secara langsung pada organisme tersebut. Jamur endofit mampu menghasilkan enzim ekstraseluler, salah satunya enzim protease. Penelitian ini bertujuan untuk mengidentifikasi jamur endofit penghasil enzim protease yang hidup pada daun mangrove Avicennia marina di Pantai Neolbaki. Metode yang digunakan adalah eksperimental dengan dua perlakuan yaitu daun tua dan daun muda mangrove A. marina. Data karakterisasi morfologi secara makroskopis dan mikroskopis dianalisis secara kualitatif sedangkan data uji aktifitas protease dari jamur endofit dengan metode plug agar berupa hasil perhitungan zona bening secara kuantitatif dan dideskripsikan dalam bentuk gambar dan tabel. Berdasarkan data penelitian ditemukan bahwa dari daun tua dan daun muda mangrove A. marina yang ada di pantai Noelbaki menghasilkan 12 total isolat hasil isolasi. Dari total 12 isolat diperoleh 5 isolat jamur endofit penghasil enzim protease ekstraseluler yang terbagi atas 3 isolat dari daun tua dan 2 isolat dari daun muda. Pembentukan zona aktifitas proteolitik tertinggi pada daun tua A. marina dalam uji enzim protease ekstraseluler terdapat pada kode isolat DT Am 05 OLL dengan diameter zona proteolitik sebesar 2,1 mm. sedangkan untuk daun muda A. marina aktivitas tertinggi terdapat pada kode isolat DM Am 02 OLL dengan diameter zona bening sebesar 1,9 mm. Kata Kunci: Avicennia marina, Enzim protease ekstraseluler, Jamur endofit. ABSTRACT Endophytic fungi can be defined as microbes that live in colonies in the internal tissues of living things such as leaves without causing direct adverse effects on these organisms. Endophytic fungi are able to produce enzymes, one of which is the protease enzymes. This study aims to identify Endophytic fungi that produce protease enzymes that live on mangrove leaves of Avicennia marina in Noelbaki Beach. The research used an experimental method with two treatments, namely old and young leaves of the A. marina mangrove. The macroscopic and microscopic morphological characterization data were analyzed qualitatively, while the protease activity test data of endophytic fungi using the plug agar and the clear zone were analyzed quantitatively, then described in the form of figures and tables. The founding of the research of the old and young leaves of A. marina from the Noelbaki beach had been isolated a total of 12 isolates of endophytic fungi. Of the 12 isolates, 5 isolates were shown to produce extracellular protease enzymes, consisting of 3 old leaves isolates and 2 young leaves isolates. In the extracellular protease enzyme test, the proteolytic activity zone was confirm as a clear zone was found to be the highest in the old leaves of A. marina found in the isolate code DT-Am-05-OLL with a proteolytic zone diameter of 2.1 mm. As for young leaves of A. marina, the highest activity was found in the DM-Am-02-OLL isolate code with a clear zone diameter of 1.9 mm. Key Words : Avicennia marina, endophytic fungi, extracellular protease enzymes
Co-Authors Agus Maramba Meha Apriliana Ballo Daud, Yanti Djawang, Jofret Umbu Soru Peku Febriana Bani Utang Fransina Nomleni Fransina Th. Nomleni Gregorius Olla Hendrik, Arnold Ch. James Ngginak Jonathans, Peggy Magdalena Jusuf Blegur Ledo, Mellissa Erlyn Stephanie Ludji Lobo, Ocsryn Lylianti Lumba, Anderias J.F. Manu, Theodora Sarlotha Nirmala Maromon, Eltina Agustina Maryana M. Atambaru Merpiseldin Nitsae Nge, Sonya T Nge, SonyaSonya Titin M. Ngede, Alfandi Nomleni, Fransina T Noprianto Umbu Tiba Marak Novi I Bullu Sabuna, Alan Ch. Thobias Hasan