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All Journal Indonesia Medicus Veterinus Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research)
Anak Agung Gde Oka Dharmayuda
Bagian Orthopaedi Dan Traumatologi RSUP Sanglah Denpasar
Agriculture, Biological Sciences & Forestry Medicine & Pharmacology Veterinary

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Identifikasi Senyawa Kimia Ekstrak Buah Naga Putih dan Pengaruhnya Terhadap Glukosa Darah Tikus Diabetes WIBAWA, PUTU ALIT SURYA; ANTARA, MADE SUMA; DHARMAYUDA, OKA
Indonesia Medicus Veterinus Vol 2 (2) 2013
Publisher : Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (213.584 KB)

Abstract

This study aims to determine the class of chemical compounds ethanol extract of white dragon fruit (H. undatus) and the effect of decreasing blood glucose levels of male white rats (Rattus norvegicus) alloxan-induced. Blood samples were taken from 25 male white rats (R.norvegicus) aged 3 months with an average weight 150-300 grams. The design used was a completely randomized design (CRD) with five treatments of the mice given no treatment (negative control), rats treated with alloxan (positive control), rats treated with alloxan + ethanol extract of white flesh dragon fruit (H. undatus) 2% I-dose (50 mg / kg bw), rats treated with alloxan + ethanol extract of white dragon fruit (H. undatus) 2% II dose (100 mg / kg bw), rats treated with alloxan + glibenclamide 0.02% (a dose of 1 ml / kg bw). Each treatment examined blood glucose levels on days 0, 3, 7, 14, and 21. Before the adapted rats treated 2 weeks and fasted for 16-18 hours. Each treatment consisted of 5 replicates. The variables measured were blood glucose levels in each treatment. The data obtained was analyzed by using analysis of variance (ANOVA) and if there is a difference between treatments then proceed with testing on Duncan test. The research shows that ethanol extract of white dragon fruit (H. undatus) 2% dose (50 mg / kg bw), doses (100 mg / kg bw) and glibenclamide 0.02% 1 ml / kg body weight can significantly reduce levels blood glucose (P <0.05) day-7 until day 21 in alloxan-induced rats. This shows the ethanol extract of meat white dragon fruit (H.undatus) can be used as a lowering of blood glucose levels.
Identification and Prevalence of Aspergillus sp Isolated from Bali Dog’s Skin Sudipa, Putu Henrywaesa; Puja, I Ketut; Dharmayuda, Anak Agung Gde Oka; Gunawan, I Wayan Nico Fajar; Sudimartini, Luh Made; Jayanti, Putu Devi; Sukernayasa, I Wayan; Mufa, Romy Muhammad Dary
Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) VOLUME 7, No 1, JANUARY 2023
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jrvi.v7i1.21594

Abstract

Aspergillus sp are fungi that commonly caused a systemic respiratory infection in Human and Dog. In Bali, most of the dog are free-roaming dogs, and has poor skin health condition. Some of them are suspected with fungal infection. Aspergillus sp has high risk to infect the dog, because they are available everywhere in environment. Therefore, this research is conducted for adding primary data of Aspergillus sp isolated from dog’s skin and their prevalence. The samples are 15 free-roaming Bali dogs with suspected fungal infection. Identification was carried out by using microscopic and macroscopic observation methods, skin swab was taken from the dog’s skin using the Mackenzie method, and the sterile toothbrush swab gently from the dog’s skin to the Sabouraud Dextrose Agar (SDA) media. The fungi were cultured on SDA media for macroscopic identification for approximately 3-4 days, mostly to observe how the fungal colony grew and its color. Microscopic identification are using adhesive tape, the tape was gently pressed into the fungal colony, the tape was stuck into the surface of object-glass with 3-4 drops of Methylene Blue staining, and the object glass was examined by using a light microscope, the findings of Aspergillus sp fungi are recorded and the data are descriptively presented. The result shows that most Aspergillus species that were found is Aspergillus fumigatus and Aspergillus niger according to their colonies growth and microscopic appearance, and the prevalence from 15 samples were 53.33%. Keywords: Aspergillus sp, fungi, Bali Dogs, Identification, Prevalence
Co-Authors Anak Agung Sagung Kendran I Ketut Puja I MADE SUMA ANTARA Ketut Tono Pasek Gelgel Luh Dewi Anggreni Luh Made Sudimartini Made Suma Anthara Mufa, Romy Muhammad Dary Ni Wayan Linda Pertiwi PUTU ALIT SURYA WIBAWA Putu Devi Jayanti Putu Henrywaesa Sudipa Sukernayasa, I Wayan