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All Journal Agrin : Jurnal Penelitian Pertanian Makara Journal of Science Kesmas: Jurnal Kesehatan Masyarakat Nasional (National Public Health Journal)
Noviyanti, Rintis
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Agriculture, Biological Sciences & Forestry Health Professions Public Health

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Detection of Papua New Guinea Thalassemia Alpha Mutation in Gayo, Sumba, Ternate, and Timika Populations Nurfitriani, Risya; Abinawanto, Abinawanto; Noviyanti, Rintis; Trianti, Lely; Nainggolan, Ita M.
Makara Journal of Science Vol. 18, No. 2
Publisher : UI Scholars Hub

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Abstract

Papua New Guinea (PNG) mutation is a point mutation that occurs in noncoding region of alpha globin clusters. Polymorphism promotes an additional recognition site for transcription factor (GATA-1) which presumably downregulates alpha globin synthesis. The aim of this research is to detect PNG mutation in other populations in Indonesia, thus the results will be used for completing standard diagnoses in detecting alpha thalassemia mutation based on ethnic background. The method used in detecting PNG mutation was PCR-RFLP. Detection of 399 samples (MCH
STRATEGI KLONING DAN EKSPRESI GEN var2csa PADA SISTEM Escherichia coli SEBAGAI LANGKAH PENDAHULUAN UNTUK EKSPRESI GEN PADA SISTEM EUKARIOT TANAMAN Hadi, Sapto Nugroho; Noviyanti, Rintis
Agrin Vol 19, No 1 (2015): Agrin
Publisher : Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.agrin.2015.19.1.351

Abstract

Penelitian ini bertujuan mengetahui apakah strategi yang digunakan dapat berhasil mengkloning danmengekspresikan sekuen DNA target (daerah DBL3x gen var2csa) pada inang E.coli. Strategi kloning dilakukanmelalui tahapan: perbanyakan sekuen DNA target melalui teknik PCR menggunakan primer yang didesain sendiri,peligasian sekuen DNA target pada vektor kloning pCR2.1-TOPO, dan memperbanyaknya pada inang E.coliTOP10. Strategi ekspresi dilakukan melalui tahapan: perbanyakan sekuen DNA target melalui teknik PCRmenggunakan primer yang mengandung situs pengenalan enzim restriksi endonuklease tipe II: NcoI dan XhoI,pemotongan dengan enzim NcoI dan XhoI, peligasian pada vektor ekspresi pET-28a (+), dan pengekspresian padainang E. coli BL21 (DE3) dengan induser isopropyl-1-thio-ß-D-galactoside (IPTG) 1 mM. Strategi kloningberhasil mendapatkan sekuen daerah DBL3x gen var2csa berukuran 948 pb. Strategi ekspresi berhasilmendapatkan protein DBL3x berukuran 38 kDa.Kata kunci: kloning, ekspresi, DBL3x, var2csa, Escherichia coliABSTRACTThe objective of this study is to see are the strategies approach that used successfully clone and expressDBL3x region of var2csa gene in E.coli host. The cloning strategies are amplifying DNA target through PCRtechnique using self design oligonucleotide primer, inserting into cloning vector pCR2.1-TOPO, copying inE.coli TOP10 host. The expression strategies are amplifying DNA target through PCR using self design primerwith specific site for restriction enzyme NcoI and XhoI, digesting with NcoI and XhoI, inserting into pET-28a (+)expression vector, and expressing in E. coli BL21 (DE3) host by adding isopropyl-1-thio-ß-D-galactoside (IPTG)1 mM as a inducer. This study successfully cloned DBL3x region of var2csa gene with 948 pb in size andsuccessfully expressed protein DBL3x with 38 kDa in size in E.coli system.Keywords: cloning, expression, DBL3x, var2csa, Escherichia coli
SARS-CoV-2 Antibody Seroprevalence in Jakarta, Indonesia Ariawan, Iwan; Jusril, Hafizah; N Farid, Muhammad; Riono, Pandu; Wahyuningsih, Wiji; Widyastuti, Widyastuti; Handayani, Dwi Oktavia T L; Wahyuningsih, Endang Sri; Daulay, Rebekka; Henderiawati, Retno; Malik, Safarina G; Noviyanti, Rintis; Trianty, Leily; Fadila, Nadia; Myint, Khin Saw Aye; Yudhaputri, Frilasita A.; Venkateswaran, Neeraja; Venkateswaran, Kodumudi; Udhayakumar, Venkatachalam; Hawley, William A.; Morgan, Juliette; Pronyk, Paul M
Kesmas Vol. 17, No. 3
Publisher : UI Scholars Hub

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Abstract

The SARS-CoV-2 transmission dynamics in low- and middle-income countries remain poorly understood. This study aimed to estimate the SARS-CoV-2 antibodies seroprevalence in Jakarta, Indonesia, and to increase knowledge of SARS-CoV-2 transmission in urban settings. A population-based serosurvey among individuals aged one year or older was conducted in Jakarta. Employing a multistage sampling design, samples were stratified by district, slum, and non-slum residency, sex, and age group. Blood samples were tested for IgG against three different SARS-CoV-2 antigens. Seroprevalence was estimated after applying sample weights and adjusting for cluster characteristics. In March 2021, this study collected 4,919 respondents. The weighted estimate of seroprevalence was 44.5% (95% CI = 42.5-46.5). Seroprevalence was highest among adults aged 30-49 years, with higher seroprevalence in women and the overweight/obese group. Respondents residing in slum areas were 1.3-fold more likely to be seropositive than non-slum residents. It was estimated that4,717,000 of Jakarta's 10.6 million residents had prior SARS-CoV-2 infection. This suggests that approximately 10 infections were undiagnosed/underreported for every reported case. About one year after the first COVID-19 case was confirmed, close to half of Jakarta's residents have been infected by SARS-CoV-2.
Co-Authors Abinawanto Abinawanto Daulay, Rebekka Endang Sri Wahyuningsih, Endang Sri Fadila, Nadia Handayani, Dwi Oktavia T L Henderiawati, Retno Ita M. Nainggolan Iwan Ariawan Jusril, Hafizah Malik, Safarina G Morgan, Juliette Myint, Khin Saw Aye N Farid, Muhammad Nurfitriani, Risya Pandu Riono Pronyk, Paul M Sapto Nugroho Hadi Trianti, Lely Trianty, Leily Udhayakumar, Venkatachalam Venkateswaran, Kodumudi Venkateswaran, Neeraja Wahyuningsih, Wiji Widyastuti Widyastuti William A. Hawley Yudhaputri, Frilasita A.