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All Journal Jurnal Ilmu Pertanian Indonesia Vegetalika Journal of Degraded and Mining Lands Management Jurnal Agroekoteknologi Proceeding Seminar LPPM UMP Tahun 2014 Planta Tropika BERITA BIOLOGI Jurnal Penelitian Pertanian Terapan Panrita Abdi - Jurnal Pengabdian pada Masyarakat Kultivasi Agrotekma: Jurnal Agroteknologi dan Ilmu Pertanian Agrin : Jurnal Penelitian Pertanian Jurnal Agrotek Tropika Dinamika Journal : Pengabdian Masyarakat Jurnal Abdi Insani Jurnal Puruhita
Sapto Nugroho Hadi
Agroecology Laboratory, Faculty Of Agriculture, Jenderal Soedirman University
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mathematics Social Sciences Other

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SKRINING MARKA RAPD TERKAIT KARAKTER MANDUL JANTAN PADA JERUK LOKAL DAN INTRODUKSI Dewi, Prita Sari; Hadi, Sapto Nugroho; Indraswari, Giriluhita
Proceeding Seminar LPPM UMP 2015: Buku I Bidang Ilmu Ekonomi dan Pertanian, Proceeding Seminar Nasional LPPM 2015, 26 September
Publisher : Proceeding Seminar LPPM UMP

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Jeruk tanpa biji merupakan salah satu karakter penting dalam program pemuliaan tanaman jeruk. Salah satu metode yang sering digunakan untuk menghasilkan jeruk tanpa biji adalah menggunakan tetua persilangan dengan karakter mandul jantan (male sterility) dan partenokarpi. Penelitian yang dilakukan oleh Dewi et al. (2013c) menunjukkan adanya segregasi karakter mandul jantan pada hibrida hasil persilangan “HY16” (Citrus hanayu × C. yuzu)  × “Foster Pink” grapefruit (C. paradisi). Ada tujuh marka RAPD terseleksi dari total 260 primer yang digunakan terkait karakter mandul jantan pada hibrida hasil persilangan tersebut. Marka tersebut meliputi CMN-A10, CMN-A19, CMN-B66, CMN-B19, CMN-B92, OPA05 dan OPB17. Peta pautan antara karakter mandul jantan dan ketujuh marka tersebut sudah dibuat dengan jarak terdekat 8,4 cM terhadap gen dominan (gen R1 atau R3) dan 15,5 cM terhadap gen resesif (r1 atau r3). Ketujuh marka tersebut digunakan untuk melakukan skrining terhadap 15 jeruk lokal dan introduksi dengan karakter fertil jantan serta karakter berbiji atau non biji. Suatu varietas jeruk dikatakan bersifat non biji bila memiliki enam biji atau kurang. Profil agarose menunjukkan bahwa tiga primer (OPA5, CMN-B19 dan CMN-B92) teramplifikasi pada semua jenis jeruk yang diuji. Hasil analisis PCR menunjukkan tiga marka yaitu CMN-B66, CMN-A19 dan CMN-A10 teramplifikasi pada semua jenis jeruk yang diuji kecuali pada jeruk nipis non biji (C. aurantifolia). Di sisi lain, ada satu marka yaitu OPB17 yang teramplifikasi hanya jeruk nipis non biji. Pengamatan morfologi terhadap bunga jeruk nipis non biji menunjukkan adanya kondisi fertil jantan. Meskipun demikian, jeruk nipis non biji ini memiliki gen dominan terkait fertil jantan dan gen resesif terkait mandul jantan.  Kata kunci : Jeruk, mandul jantan, marka RAPD, pautan sifat.
GENETIC DIVERSITY OF POTATO BASED ON RANDOM AMPLIFIED POLYMORPHIC DNA AND SIMPLE SEQUENCE REPEAT MARKER Hadi, Sapto Nugroho; Nurchasanah, Siti
PLANTA TROPIKA: Jurnal Agrosains (Journal of Agro Science) Vol 8, No 1 (2020)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.2020.114.54-62

Abstract

Various potato clones are cultivated by farmers in Banjarnegara and Wonosobo, Central Java, Indonesia such as MZ, NH1, NH2, Vega, Gareta, Granola, Bliss, Merah (Red Potato), Ungu (Purple Potato), Klon_17 (K17), Local Dieng, Margahayu, and X. This encourages the importance of genetic diversity and genetic similarities. This study aimed to analyze genetic diversity and genetic similarities between 13 accessions of potatoes based on seven Random Amplified Polymorphic DNA (RAPD) primers and nine pairs of Simple Sequence Repeat Marker (SSR) primers. The results showed that RAPD and SSR primers could be used to analyze genetic diversity and genetic similarities of 13 potatoes accessions from Banjarnegara and Wonosobo. The PLP value was 80.9% in the RAPD primer and 65.41% in the SSR primer. Four RAPD primers were informative based on PIC value: OPG 08, OPM 19, OPP 13, and OPX 04. Three SSR primer were informative: STM 2005, RGH- SSR 8, and StI 035. Genetic similarities presented by Phylogenetic tree analysis resulted in two main clusters. The first cluster consisted of Granola, MZ, Ungu (Purple potato), Merah (Red Potato), Local Dieng, Margahayu, Gareta, Vega, NH2, NH1, Klon_17 (K17), and Bliss. The second cluster consisted of X. Granola and MZ having a high genetic similarity with a genetic distance of 0.07 and 0.132. Meanwhile, K17 and X had a low genetic similarity with a genetic distance of 0.31 and 0.987.
PENGEMBANGAN METODE EKSTRAKSI DAN ANALISIS HORMON Indole-3-butyric acid DARI TANAMAN KENTANG (Solanum tuberosum L.) MENGGUNAKAN HIGH PERFORMANCE LIQUID CHROMATOGRAPHY Hadi, Sapto Nugroho; Musthafa, Muhammad Bachtiar; Faozi, Khavid; ., Saparso
Jurnal Agroekoteknologi Vol 7, No 1 (2015)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (23.242 KB) | DOI: 10.33512/j.agrtek.v7i1.608

Abstract

Studying plant growth hormone such as Indole-3-butyric acid (IBA), one of auxin family is important for potato seed development. Unfortunately, simple, quick, and accurate extraction and analysis method to support the study of hormone in potato plant still limited. This study aim to develop extraction and analysis method of IBA from potato fresh tissue. For analysis method development, this study used IBA standard (purity 99.9%) as sample. The Analysis used reversed-phase high performance liquid chromatography (RP-HPLC) with ultraviolet detector (wavelength 280 nm), C18 column, and room temperature for chromatography condition. To get the best analysis method, this study used methanol, water, and acetic acid as mobile phase with several different compositions. This study also used several different flow rate of mobile phase. The study tested the best analysis method by linearity standard curve procedure. The concentrations of the standard IBA solution for linearity were 50 ppm, 100 ppm, and 150 ppm. This study used the best analysis method to confirm extraction result of IBA hormone from potato fresh tissue by maceration procedure. Several compositions of methanol and temperatures used to find the best extraction procedure of the hormone. The result showed for the best analysis method by RP-HPLC, the composition of mobile phase was methanol: water = 80: 20 (v/v) with 0.8 ml/min in flow rate. The result showed, the best analysis method of IBA has linearity equation: y = 7175x + 17734 with correlation coefficient value (R2) = 0.992. For the best extraction method of IBA, the best composition of sample and methanol was 2.5 gram: 10 ml (b/v) under controlled temperature (10oC).  The conclusions, the studies were successfully developing extraction and analysis method of IBA hormone from potato fresh tissue.
APLIKASI TEKNOLOGI BUDIDAYA LADA PERDU DI BAWAH TEGAKAN TANAMAN PEPAYA DI DESA PATEMON PURBALINGGA JAWA TENGAH Hadi, Sapto Nugroho; Harjoso, Tri
Jurnal Puruhita Vol 1 No 1 (2019): February 2019
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/puruhita.v1i1.28309

Abstract

Kegiatan ini bertujuan untuk menerapkan teknologi budidaya lada perdu secara tepat sebagai tanaman sela di bawah tegakan tanaman pepaya untuk optimalisasi lahan dan peningkatan pendapatan petani di Desa Patemon, Kecamatan Bojongsari, Kabupaten Purbalingga, Provinsi Jawa Tengah. Mitra kegiatan adalah Kelompok Tani “Kubang Tani”. Metode yang digunakan adalah 1). Transfer teknologi dan pendampingan yang melibatkan penyuluh pertanian di Kecamatan Bojongsari mengenai teknologi budidaya lada perdu sebagai tanaman sela, 2). Pembuatan demonstrasi dan plot (demplot) seluas 100 ubin (1400 m2), dan 3). Pelatihan pembuatan pupuk organik dari kotoran hewan sapi. Hasil yang diperoleh dari kegiatan ini: 1). Wawasan petani terkait teknologi budidaya lada perdu sebagai tanaman sela meningkat, 2). Petani mendapatkan pengalaman praktik langsung teknik budidaya daya lada perdu yang tepat menggunakan 200 bibit unggul, pupuk berimbang, sistem pemeliharaan yang baik seperti penyiangan gulma, penyulaman, dan pengairan, 3). Demplot seluas 1400 m2 sebagai percontohan untuk penerapan teknologi budidaya lada perdu, dan 4). Petani mendapat pengalaman dan keterampilan memanfaatkan sumberdaya lokal seperti kotoran sapi untuk dijadikan pupuk organik.
Pengaruh bahan organik berbasis gulma paitan dan pupuk NPK terhadap sifat kimia tanah, pertumbuhan, dan hasil tomat pada Ultisols Intan Abas Nur Khairunnisa; Anung Slamet Dwi; Sapto Nugroho Hadi
Kultivasi Vol 18, No 3 (2019)
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (165.692 KB) | DOI: 10.24198/kultivasi.v18i3.21140

Abstract

Sari. Penelitian ini bertujuan untuk mengetahui pengaruh bahan organik gulma paitan dan pupuk NPK terhadap sifat kimia tanah (C organik, N, dan P total), serta pertumbuhan dan hasil tomat pada Ultisols.  Penelitian dilaksanakan di screen house Fakultas Pertanian, Universitas Jenderal Soedirman, Purwokerto, pada bulan Juni sampai September 2018. Penelitian ini menggunakan rancangan perlakuan faktorial 3x3. Faktor pertama adalah bahan organik tumbuhan paitan, terdiri atas 3 taraf, yaitu 0, 125, dan 250 g/tanaman. Faktor kedua adalah dosis NPK, terdiri atas 3 taraf, yaitu 0; 3,61; dan 5,42 g/tanaman. Seluruh kombinasi perlakuan dialokasikan ke dalam unit percobaan dengan menggunakan Rancangan Acak Kelompok yang diulang 3 kali. Variabel yang diamati meliputi C organik, N total, P total, serta pertumbuhan dan hasil tomat. Data dianalisis dengan uji F, dilanjutkan dengan Duncan’s Multiple Range Test. Hasil penelitian menunjukkan bahwa bahan organik tumbuhan paitan dengan dosis 250 g/tanaman cenderung memperbaiki sifat kimia tanah, serta berpengaruh terhadap pertumbuhan dan hasil tomat seperti jumlah daun, luas daun, bobot segar buah per tanaman sebesar 43,29% dari kontrol, dan jumlah buah sebesar 54,26% dari kontrol. Pupuk NPK dengan dosis 5,42 g/tanaman cenderung berpengaruh terhadap sifat kimia tanah, serta berpengaruh nyata semua variabel pertumbuhan dan hasil kecuali bobot segar akar. Kombinasi bahan organik tumbuhan paitan dengan dosis 250 g/tanaman dan pupuk NPK dengan dosis 5,42 g/tanaman cenderung berpengaruh terhadap sifat kimia tanah dan berpengaruh nyata terhadap variabel luas daun.Kata Kunci: Tithonia diversifolia, pupul NPK, Tomat, Ultisols Abstract. This research aims to know effect of organic matters of Tithonia diversifolia weeds and NPK fertilizer to soil chemical properties (organic carbon, total N, and total P), growth, and yield of tomato in Ultisols soil. The research was conducted at Screen House of Faculty of Agriculture, Jenderal Soedirman University, Purwokerto regency, from June to September 2018. This research used 3x3 factorial treatment design. The first factor was the dosage of organic matters of Tithonia diversifolia, consisted of 3 levels: 0, 125, dan 250 g/plant. The second factor was the dosage of NPK fertilizer, consisted of 3 levels: 0, 3.61, and 5.42 g/plant. All of treatment combinations were allocated to the experimental unit that used Randomized Block Design and was replicated 3 times. The variables observed were organic C, total N, total P, growth, and yield of tomato. Observational data were analyzed by F test, then Duncan's Multiple Range Test. The results showed that dosage 250 g/plant of organic matter of Tithonia diversifolia improved soil chemical properties, and affected significantly to growth, and yield variable likes number of leaves, wide of leaves, number of fruit, and fruit fresh weight. Dosage 5.42 g/plant of NPK fertilizer improved soil chemical properties, and affected significantly to all variables of growth, and yield, except root fresh weight. Combination of Tithonia diversifolia organic matter dosage 250 g/plant and 5,42 g/plant NPK fertilizer improved soil chemical properties and affected significantly to leaves area.Keywords: Tithonia diversifolia, NPK fertilizer, Tomato, Ultisols
Karakter Morfo-fisiologi dan Hasil Padi Gogo Toleran Kekeringan Yugi Rahayu Ahadiyat; Sapto Nugroho Hadi; Okti Herliana
Jurnal Ilmu Pertanian Indonesia Vol. 25 No. 3 (2020): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.25.3.462

Abstract

The objective of this study was to obtain varieties with high yield along with proper morpho-physiological characters under drought condition. Eleven varieties of upland rice i.e., Gilirang, Situ Patenggang, Kalimutu, Aek Sibundong, Batutegi, Towuti, Sunggal, Danau Gaung, Way Apo Buru, Danau Tempe, and Situ Bagendit were characterized based on morphology, physiology, and yield. Non factorial Complete Randomized Block Design was used with three times repetition. Parameters measured were morphological characters, physiological characters, and yield components. Morphological characters measured were plant height, number and area of leaf, shoot dry biomass, tiller number, and total root length. Physiological characters measured were plant growth rate, net assimilation rate, and relative growth rate. Yield components measured were number and length of panicle, number of seeds per panicle, 1000 seeds weight, weights of seed per panicle, weights of seed per plot, and weights of seed per hectare. The results showed that Danau Tempe variety had better characters against drought stress compared to the other varieties with higher yields supported by high plant growth rate, relative growth rate, chlorophyll content and leaf area with low accumulation of proline content and short root length. Keywords: drought, morphological character, upland rice variety, yield
PENERAPAN BUDIDAYA PADI DENGAN METODE SRI (SISTEM OF RICE INTENSIFICATION) DI DESA PATEMON KECAMATAN BOJONGSARI KABUPATEN PURBALINGGA Okti Herliana; Sapto Nugroho Hadi; Wilis Cahyani
Dinamika Journal : Pengabdian Masyarakat Vol 1, No 3 (2019)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.dj.2019.1.3.899

Abstract

Patemon Village is located in the District of Bojongsari, Purbalingga Regency, approximately 17 Km from the General Soedirman University Campus with a total of 528 Family Heads and a population of 4350 people. The livelihoods of the population are mostly farmers. Agricultural potential of Patemon Village, Bojongsari Subdistrict includes: rice, corn, soybeans and cassava. The area of paddy fields in this village reaches 128,000 ha but unfortunately its productivity is still relatively low. This is because farmers are still implementing conventional cultivation systems and lack of knowledge about integrated crop management. This activity aims to provide knowledge and examples of alternative rice cultivation methods, namely rice farming using the SRI (System of Rice Intensification) method with the application of straw compost and vegetable pesticides. This activity involved the target audience, namely the "Warakan Tani" farmer group in Patemon Village, Bojongsari District and agricultural instructors in Bojongsari sub-district. The methods used in this activity are: 1. Direct extension activities to farmer groups 2. Direct practice / rice cultivation demonstration plot using SRI (System of Rice Intensification) method or making demonstration plots. The evaluation design is in the form of providing pre-test before the extension activity begins and post-test after the extension activity begins, this is intended to determine the extent of farmers' understanding of the SRI method.
Phytoremediation of cadmium-contaminated soil using terrestrial kale (Ipomoea reptans Poir) and corncob biochar Ika Fitriana Dyah Ratnasari; Sapto Nugroho Hadi; Slamet Rohadi Suparto; Okti Herliana; Yugi R Ahadiyat
Journal of Degraded and Mining Lands Management Vol 7, No 4 (2020)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2020.074.2313

Abstract

This study aimed to examine the potential of terrestrial kale (Ipomoea reptans Poir) combined with corncob biochar for phytoremediation of cadmium-contaminated soil. The experiment design was a completely randomized block design with two factors. The first factor was the population density of Ipomoea reptans (0, 2, and 4 plants/polybag). The second factor was the dose of corncob biochar (0, 5, and 10 t corncob biochar/ha). The variables observed were plant height, leaf number, leaf area, chlorophyll content, wet shoot weight, dry shoot weight, wet root weight, dry root weight, effectiveness of plant in Cd absorption and removal efficiency of Cd. The results showed that Ipomoea reptans could adsorb 73.59% of Cd without application of corncob biochar. Ipomoea reptans planted with a population density of 4 plants/polybag reduced Cd content in the soil by 57.70% Application of 10 t corncob biochar/ha reduced Cd content in the soil by 43.42%. There was an interaction between Ipomoea reptans panted with a population density of 4 plants/polybag with the application of 10 t corncob biochar that reduced Cd content in the soil by 62.42%.
Application of wood vinegar coconut shell and NPK fertilizer to maintain sustainable agriculture of upland rice production Yugi R Ahadiyat; Sapto Nugroho Hadi; Okti Herliana
Journal of Degraded and Mining Lands Management Vol 5, No 3 (2018)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (82.584 KB) | DOI: 10.15243/jdmlm.2018.053.1245

Abstract

Objective of this study was to know the effect of NPK fertilizer and wood vinegar coconut shell on upland rice yield and, pest and disease intensity. Inpago Unsoed 1 was a main object in this study. Application of different dose of NPK as main plot viz. 50% and 100% recommended dose and concentration of wood vinegar coconut shell of ratio i.e. 1: 20, 1: 40, 1: 60, 1: 80 and 1: 100 were tested. Observation variables were number of panicle per hill, number of seed per hill, percentage of filled seed, seed weight per hill, weight of 1000 seeds and weight of seed per effective plot. Application of wood vinegar coconut shell with concentration of 1:20 improved grain yield of upland rice and reduced 50% NPK application, and suppress intensity of pest and disease.
STATUS KESEHATAN TANAH PERTANIAN CABAI ORGANIK DAN KONVENSIONAL: TINJAUAN KELIMPAHAN POPULASI BAKTERI, ENZIM TANAH DAN POTENSI RHIZOBAKTERI SEBAGAI PEMACU PERTUMBUHAN TANAMAN (RPPT) Agung Adi Nugroho; Tirta Kumala Dewi; Risa Okiana Safitri; Sapto Nugroho Hadi; Entis Sutisna; Nani Mulyani; Sarjiya Antonius
BERITA BIOLOGI Vol 21, No 3 (2021): Berita Biologi
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v21i3.4202

Abstract

Cabai merupakan komoditas pertanian dengan nilai ekonomi cukup tinggi. Budidaya cabai konvensional cenderung menggunakan bahan agrokimia berlebihan yang dapat menimbulkan dampak negatif. Tujuan penelitian ini adalah membandingkan kesehatan tanah pada budidaya tanaman cabai organik dan konvesional berdasarkan parameter populasi bakteri RPPT dan aktivitas biokimia tanah serta mengidentifikasi potensi RPPT sebagai kandidat pupuk hayati. Populasi bakteri RPPT dan aktivitas biokimia tanah pertanian cabai organik lebih tinggi dibandingkan konvensional. Populasi bakteri RPPT tanah cabai organik meliputi total bakteri, bakteri pelarut P, penghasil IAA, proteolitik dan penambat N2 lebih tinggi yaitu berkisar 1,06 x 106 - 25,15 x 106 cfu/g dibandingkan tanah cabai konvensional 0,34 x 106 – 7,46 x 106 cfu/g. Aktivitas enzim tanah cabai organik seperti enzim fosfomonoesterase, urease dan respirasi yaitu 130,73 μg pNP g-1 jam-1; 165,65 µg N-NH4 g-1 jam-1; 0,14 CO2mg tanah/jam, secara berurutan. Aktivitas RPPT dari tanah cabai organik memiliki potensi pelarut P dan penghasil IAA yang cukup tinggi. Kandungan N tanah pertanian organik dan konvesional  tidak banyak berbeda, tetapi kandungan C dan P lebih tinggi pada tanah pertanian cabai organik, sedangkan kandungan K cenderung lebih rendah. Secara keseluruhan kesehatan tanah berdasarkan populasi bakteri, biokimia tanah dan sifat kimia tanah cabai organik lebih baik dibandingkan konvensional.
Co-Authors ., Saparso Afandi, Dadang Agung Adi Nugroho AHADIYAT YUGI R. Ahmad Fauzi Anung Slamet Dwi Cahyani, Wilis Entis Sutisna Faozi, Khavid Fatichin, Fatichin Fauzi Ahmad Muda Giriluhita Indraswari, Giriluhita Herliana, Okti Ida Widiyawati Ida Widiyawati Ika Fitriana Dyah Ratnasari Indra Syahputra Intan Abas Nur Khairunnisa MARTIANSYAH, Irfan Mukmin, Restu Prasetya Musthafa, Muhammad Bachtiar Nani Mulyani Noviyanti, Rintis Nurchasanah, Siti Okti Herliana Okti Herliana, Okti Prita Sari Dewi PUTRANTO, Riza Arief Risa Okiana Safitri Rostaman Sari, Dini Astika Sarjiya Antonius Slamet Rohadi Suparto Syaeful Anwar Tamad, Tamad Tirta Kumala Dewi Totok Agung Dwi Haryanto TRI HARJOSO Wilis Cahyani Wilis Cahyani