Claim Missing Document
Check
Articles

Found 2 Documents
Search
Journal : Jurnal Silvikultur Tropika (JST)

Isolation and Selection of Potential Isolates of Dark Septate Endophyte Fungi from Pinus merkusii Roots Akhir, Jumadil; Budi, Sri Wilarso; Herliyana, Elis Nina; Surono
Journal of Tropical Silviculture Vol. 14 No. 03 (2023): Jurnal Silvikutur Tropika
Publisher : Departemen Silvikultur, Fakultas Kehutanan dan Lingkungan, Institut Pertanian Bogor (IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/j-siltrop.14.03.236-241

Abstract

Root isolation activities to obtain dark septate endophytic fungi are currently mostly carried out on agricultural and plantation crops, while less so on forestry crops. Roots are a suitable habitat for the growth of various microorganisms, including dark septate endophytic fungi. The research aims to isolate the roots of Pinus merkusii plants from four locations, test pathogenicity, and test the biomass of sweet caisim mustard seeds. The research used a descriptive method by observing the number of dark septate endophytic fungal isolates obtained and the pathogenicity of these isolates. Meanwhile, sweet ciasim mustard seed biomass from the pathogenicity test results of non-pathogenic isolates was analyzed using a completely randomized design. The results of root isolation from Pinus merkusii stands obtained as many as 25 isolates by isolating 1,160 root segments. Isolation of Pinus merkusii roots from the IPB Dramaga Campus obtained 16 isolates, Dabun Gelang Village, Gayo Lues Regency obtained 2 isolates, Gunung Walat University Forest obtained 2 isolates, and Gunung Halimun Salak National Park obtained 5 isolates. Pathogenicity tests showed that 12 isolates were pathogenic and 13 isolates were non-pathogenic. Biomass analysis of sweet ciasim mustard seeds on non-pathogenic isolates obtained the highest biomass in the control treatment (not inoculated) and the lowest in isolates Apg 23.5 and Hs 14.6b. There is still a lack of isolates obtained from isolation activities in this research, so it is necessary to find an appropriate sterilization method to obtain the maximum number of isolates and have the potential to become non-pathogenic isolates. Keywords: Pinus merkusii, dark septate endophyte, isolation
The Potential of Dark Septate Endophyte Fungi as a Biological Control of Teak Leaf Blight Pathogen in Vitro Muhammad Alam Firmansyah; Wulan Fitri Sagita; Firmansyah, Muhammad Alam; Surono; Sagita, Wulan Fitri
Journal of Tropical Silviculture Vol. 15 No. 03 (2024): Jurnal Silvikutur Tropika
Publisher : Departemen Silvikultur, Fakultas Kehutanan dan Lingkungan, Institut Pertanian Bogor (IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/j-siltrop.15.03.293-299

Abstract

Leaf blight that attacks teak (Tectona grandis L.f.) can reduce the plant's productivity. Leaf blight control can be done through dark septate endophyte (DSE). This study aims to test the ability of DSE GS2 and DSE TM fungi to inhibit the growth of pathogen colonies that cause leaf blight in vitro. Antagonistic tests of DSE against pathogen that cause leaf blight were carried out through a modified double culture test. The results of the antagonistic test showed that DSE GS2 and DSE TM could inhibit the growth of pathogens. The best inhibition results were demonstrated in the second double culture test after DSE was grown for seven days because DSE could grow stably and produce metabolite compounds. DSE TM had the best inhibition compared to DSE GS2. The mechanism of DSE inhibition against pathogens occurs through physical contact and the production of metabolite compounds characterized by the inhibition zone around the DSE. Keywords: Biocontrol, colony, Preventive, Rhizoctonia sp.