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Journal : JURNAL ATOMIK

POTENSI AKTIVITAS ANTIOKSIDAN EKSTRAK METANOL BUNGA ASOKA (Ixora coccinea L.) Ramadhan, Aulia; Saleh, Chairul; Hairani, Rita; Ruga, Ritbey
JURNAL ATOMIK Vol 9 No 1 (2024)
Publisher : Jurusan Kimia FMIPA UNMUL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/ja.v9i1.1273

Abstract

Extraction of asoka (Ixora coccinea L.) flowers, phytochemical screening, and antioxidant activity of methanol extract of asoka flowers have been conducted. This study aimed to determine the content of secondary metabolites in the methanol extract of asoka flowers and its antioxidant activity using the DPPH (2,2-diphenyl-1-picrylhydrazyl) method. The phytochemical screening test revealed that the methanol extract of asoka flowers contained secondary metabolites such as flavonoids, alkaloids, triterpenoids and saponins. With 1 mg/mL, the methanol extract exhibited potential as an antioxidant with an inhibition percentage of 88.10% while vitamin C as a standard used in this study at 1 mg/mL showed an antioxidant activity of 99.23%.
PENGHAMBATAN AKTIVITAS α-GLUKOSIDASE DARI EKSTRAK METANOL BUNGA BUGENVIL (Bougainvillea glabra Choisy) Puspitasari, Intan Dessy; Saleh, Chairul; Hairani, Rita; Ruga, Ritbey
JURNAL ATOMIK Vol 9 No 1 (2024)
Publisher : Jurusan Kimia FMIPA UNMUL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/ja.v9i1.1276

Abstract

The test of Phytochemical screening and antidiabetic activity of methanol extract of bugenvil (Bougainvillea glabra Choisy) flower against α-glucosidase inhibition have been conducted. This study aims to determine the content of secondary metabolite compounds and the effectiveness of the methanol extract of bugenvil flower against α-glucosidase activity. Phytochemical test showed that the methanol extract contained secondary metabolite compounds namely alkaloids, flavonoids, steroids, phenolics and saponins. By 50 µg/mL, the methanol extract of bugenvil flower can inhibit α-glucosidase activity with a percent inhibition value of 79.42% while acarbose as a positive control in this study exhibited antidiabetic activity against α-glucosidase of 99.30% at a concentration of 0.00006 µg/mL.
SKRINING FITOKIMIA DAN UJI TOKSISITAS MENGGUNAKAN BRINE SHRIMP LETHALITY TEST (BSLT) DARI EKSTRAK DIKLOROMETANA RIMPANG TEMU KUNCI (Boesenbergia rotunda) Jonathan, Jonathan; Ruga, Ritbey; Hairani, Rita
JURNAL ATOMIK Vol 9 No 2 (2024)
Publisher : Jurusan Kimia FMIPA UNMUL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/ja.v9i2.1407

Abstract

The rhizome of Boesenbergia rotunda (temu kunci) is one of medicinal plants having potential for medicinal, supplementary food and drink due to its biological activities. The aims of this research are to identified the secondary metabolite compounds present in the dichlorometane extract of finger-root rhizomes through phytochemical screening using thin layer chromatography (TLC) method and to investigated its toxicity against Artemia salina L. shrimp larvae by using Brine Shrime Lethality Test (BSLT). The result of phytochemical analysis showed that the dichloromethane extract contains secondary metabolite compounds including flavonoids, phenolics and triterpenoids while the toxicity test of the extract showed its LC50 value of 303.68 µg/mL. It was indicated that the extract could be classified as toxic.
AKTIVITAS ANTIOKSIDAN TUMBUHAN GENUS MANGIFERA DENGAN METODE DPPH (1,1-diphenyl-2-picrylhydrazyl) : MINI REVIEW Jannah, Nurul; Hairani, Rita; Marliana, Eva
JURNAL ATOMIK Vol 9 No 2 (2024)
Publisher : Jurusan Kimia FMIPA UNMUL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/ja.v9i2.1394

Abstract

Mangifera is a genus of the Anacardiaceae family of mangoes spread across Sumatra, Kalimantan, Java, and Bali. Several species of the genus Mangifera have been extensively researched on phytochemistry and antioxidant activity using the DPPH (1,1-diphenyl-2-picrylhydrazyl) method. The study explains that several species of the genus Mangifera contain secondary metabolite compounds, such as flavonoids, phenolics, tannins, saponins, alkaloids, triterpenoids, and quinones. The results of several studies explain that the Mangifera genus has antioxidant activity with an inhibitory concentration (IC50) value generally <50 ppm, which is a strong category, so it has the potential to be an antioxidant. Keywords : Mangifera, phytochemical screening, antioxidant activity
ISOLATION OF LIPASE PRODUCING BACTERIA FROM HOT SPRINGS OF LONG KALI Alawiyah, Dessy Noor; Astuti, Winni; Hairani, Rita
JURNAL ATOMIK Vol 6 No 1 (2021)
Publisher : Jurusan Kimia FMIPA UNMUL

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Isolasi bakteri penghasil lipase dari sumber air panas Long Kali dilakukan dengan menyeleksi koloni bakteri pada media Nutrient Agar (NA) yang mengandung Rhodamin B dan minyak zaitun. Hasil isolasi menunjukkan 5 dari 14 isolat bakteri mampu menghasilkan lipase. lima isolat tersebut diuji aktivitas lipase dengan metode titrimetri, isolat dengan kode 14 memiliki aktivitas tertinggi dengan aktivitas 2,20 Unit/mL.
AKTIVITAS ANTI-Staphylococcal SECARA IN VITRO EKSTRAK DIKLOROMETANA DARI RIMPANG TEMU KUNCI (Boesenbergia rotunda) TERHADAP Staphylococcus aureus ATCC 25923 Matutu, Deni Ari; Hairani, Rita; Ruga, Ritbey
JURNAL ATOMIK Vol 10 No 1 (2025)
Publisher : Jurusan Kimia FMIPA UNMUL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/ja.v10i1.1404

Abstract

The rhizome of temu kunci (Boesenbergia rotunda) is one of the plants used by the local people in Indonesia as a traditional medicine. This study aimed to identify the secondary metabolite compounds contained in the dichloromethane extract with phytochemical screening and investigate its antibacterial activity against test bacteria Staphylococcus aureus ATTC 25923 was carried out by agar diffusion method with modification where tetracycline as a positive control and ethanol as a negative control. The phytochemical screening showed the dichloromethane extract contains secondary metabolites such as alkaloids, flavonoids, phenolics and triterpenoids. With 1 mg/mL of dichloromethane extract revealed its antibacterial activity against S. aureus with inhibitory zone value of 15.0 ± 0.0 mm while its MIC value of the extract was 0.031 ± 0.0 mg/mL toward of tested bacteria. In addition, tetracycline as a positive control at 0.5 μg/mL exhibited its antibacterial potential with an inhibitory zone value of 22.0 ± 0.0 mm and its MIC value of 0.016 ± 0.577 µg/mL.